LOCUS       NZ_CP046340             3898 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Burkholderia multivorans strain FDAARGOS_719 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP046340
VERSION     NZ_CP046340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056434
            Assembly: GCF_009730035.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia multivorans
  ORGANISM  Burkholderia multivorans
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Coverage                   :: 364.21x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 02:45:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,942
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,786
            CDSs (with protein)               :: 5,786
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 48 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 18 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Burkholderia multivorans"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_719"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_719"
                     /db_xref="taxon:87883"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MN"
                     /collected_by="Mayo Clinic"
     gene            join(3709..3898,1..341)
                     /locus_tag="FOC27_RS29835"
                     /old_locus_tag="FOC27_29830"
     CDS             join(3709..3898,1..341)
                     /locus_tag="FOC27_RS29835"
                     /old_locus_tag="FOC27_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            775..1806
                     /locus_tag="FOC27_RS29840"
                     /old_locus_tag="FOC27_29835"
     CDS             775..1806
                     /locus_tag="FOC27_RS29840"
                     /old_locus_tag="FOC27_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_155826332.1"
                     /translation="MSAALQYFEENLPRRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWMVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKI"
                     /name="replication initiation protein"
     gene            complement(2169..2564)
                     /locus_tag="FOC27_RS29845"
                     /old_locus_tag="FOC27_29840"
     CDS             complement(2169..2564)
                     /locus_tag="FOC27_RS29845"
                     /old_locus_tag="FOC27_29840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            2744..3697
                     /locus_tag="FOC27_RS29850"
                     /old_locus_tag="FOC27_29845"
     CDS             2744..3697
                     /locus_tag="FOC27_RS29850"
                     /old_locus_tag="FOC27_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001360358.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     Typing          776..1551
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.327"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          2745..3694
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaacggacat tgcggggctg atgggtgagc gcatgaccca ggattttcag cgtcttgaag
       61 agactgtggc aaagatgaga gccaacgcgg aggcgcttaa caaggacatt gcgacaattc
      121 atcacaatgt tgttaaagaa tacaacacgc tcaataaata cagtctgaaa tggtttatcg
      181 gtgttacggt cagttttctg gtcatgttct cgttgcttgg ctggtttacc aaaacgttga
      241 tcgacaggaa ctacaccgcg acggaggtcg tgtatcagaa aattgttgat attgaaaaga
      301 aactggacaa accagcaaca gggaagagcc ggaaaaactg acgcgcttcg cttgtccaac
      361 gcctcacggg acgcgaaaaa attccgcgtc ccgtgaggcg ttttctgatg agaaagtttg
      421 ctgttttgcc ccgttcgccc ttctgaacgc tcgaaacaga tcgcgcattg ctttcgtgaa
      481 tagttatgca ggctcatgaa aacgattctg acgcgttttt ctggctttgc ctggtgtttc
      541 cttgtctttt tgcgtttttt gcgccagaaa gcgattgagg gcgttttaag gggtacgtac
      601 aacaggagtt atggtaaatg gatcgggttt gcgggaagac tcgacaggtt ttgtcgttgg
      661 gggtagtgta agcgactgaa aaacaaacgc ccctgaaatt cgtgtctatc cgccaagact
      721 gtcacgaaaa tacaggggcg atgtcggtcg ataacctgga aggaatcgta acacatgagc
      781 gccgcgcttc aatacttcga agaaaattta ccccgccgtc cctatcacac ggatgatctc
      841 gcttttggtc ttcgcatctc cggcaaaggg cgtgcgctgc ttgcgcggta catccagcag
      901 aaccagcctc atgcgcagtt ctggatggtt tttgacgttg accgcgaggg ggcagcgatt
      961 gactggagcg accggaacgc ccccgcgccg aacatcaccg ttaaaaatcc tgtgaacgga
     1021 cacgctcacc tgctctatgc actcaatatc gccgtgagaa ccgcgcctga tgcgtcggtt
     1081 aaggcgctga aatatgccgc agcggttgaa cgttcgctgt gtgagaaact ttgtgcggat
     1141 gtgaactaca gcggcttgat ttgcaaaaat ccgttccacc tggaatggct ggtgatggag
     1201 tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
     1261 gcgcgtcgta gcatcgataa acattatggg atggggcgaa actgccacct gttcgaaatg
     1321 acgcgcaaat gggcttacag ggcgattcgt cagggctggc cagcattctc acagtggctt
     1381 gatgccgtga tccagcgtgt cgaaatgtat aacgcatcgc ttcccgttcc gctttcaccg
     1441 gctgaatgtc gggctattgg caagagcatt gcgaaataca cgcacaggaa cttcacgccg
     1501 gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc cgcacgaggt
     1561 cgcaagggcg ggaaggccaa ttcgtcaaaa aatcaggcgg acaaagggaa agttggcggg
     1621 aaaaacagcg gtgttgtccg gtggacggct aacgatgata aacgtcgtag ggcgcttgat
     1681 atgtacatct tgggagcgtc aacagaagat attgcagtgg cggttggtgt tagccgttgg
     1741 actgttcgac gctggatgga tagctctgta gaatggctac tgagtaaaca aataatcaaa
     1801 atttaattaa caaaaagaat acataccccc ccctgttttg ttagtttttt gttaattttg
     1861 ggtgcacgaa gcaagcctat atcagataac agcgcctgaa cgggggtttg gtgttttttt
     1921 gcgcggcgtc tccgacccgc tcccacaccc ccaaaccaaa tnccnnancc ctncnnaccc
     1981 agcgagtggt ggctccgctc gccgcagccc tagcgaccga gccgtcaggc tcgcgagtgg
     2041 gcgaggaagc ggaagataat cggaagtcac attgagcact tacgcaccta tgccagatcc
     2101 gcgcgtagcg cgtcgcgaaa tcgaaaacat tgaacaccgg caggcaaacg caccaccacg
     2161 cagcggaatt aatgatgatc tgggcttgtt tcaagatcga gcctgattgc cttaagtttc
     2221 tgtaatatat tgtcttttgg gtatctcagt tcgcctgttt ttgtgtccac tagtccggcc
     2281 tcgatcagta accccgccgc aagaaacagg cggtgattgc gttcccgtcg ttgtgttttc
     2341 ccctccatcc gtttttggtt ggcctgtttg cgagcgttct cctgtgcctg tagctcgttt
     2401 ttttctgcgg ttgccaggac gtggagtagt ttgcgctcct gttctgttag tccggcttcg
     2461 ttatcgagca ggtcaatgat ctgcctctgt cgtgcggtct gttctccgag cttcctgata
     2521 aagccgattc gtttcttcaa ccagtcgatg ttgctgatgc gcatgtttgg gatgctccct
     2581 ggtgtttttt gcggtgtgtt ggggttgtgc agtatatcgc ttgcggtggg tgggtgcagg
     2641 ggggtatctt ccgtgctaat tcgtaaaatt agcaggaagc gcacttatat gtttcttcgc
     2701 cgttggctca gaaacatgtg cgctcctgcg gagggctaac accgtggcaa cctatcacct
     2761 caatgtaaaa tttggcggca agggacaggc cggaaaccat gccgattaca tcgaacgcaa
     2821 aggcgagtac gagaaacgaa aagatctgga atactcagag cacgggaata tgccaggctg
     2881 ggcgcgagat aatccctccc atttctggca agcggctgat cagtttgaac gtgcgaatgg
     2941 ttcgacttat cgggagatcg aaatcgcctt gccgcgtgaa ctgacaccgg atcagcgcat
     3001 tgaactggtg caggattttg tgcgtcaggt agctggagaa cggcacgcct atagctttgc
     3061 cattcacaat ccaaaagcga gcattgacgg aggggaacag cctcatgcgc atatcatgat
     3121 gagccagcgt attaacgatg gtatggaacg ttcgccggaa cagtatttcc ggcgttataa
     3181 cgccaagaat cccgaacggg gaggcgcaaa aaaagattca ggcaatctga caccgactca
     3241 gcaaaaagag caattgcgga cgttacgggc gagctggtca gcgttacaga acgagcattt
     3301 agcgcgtcat ggtcacaggg tgcgcgttga ccatcgctca ctggaagaac gcggaattaa
     3361 acgcacgcca gaacggcatt tcggatttgc cgcctcatca cggcttgatc atgcgcagcg
     3421 ccgcgaaatc ctgcaaaacc gagcgcagcc gctcgaacat tttctagaga aagcgcattc
     3481 ggttcgccat caatttgatg cacaagttca gcaaaaagag ttagttaaaa cgcttttaca
     3541 ggacgtcgca cgggagcgag ggcacgcgct tacggcaaga ataaataaaa caggtgaagt
     3601 tgaatttgaa cgtgcgccaa cgcggacgcg gagtaacacg cgagggcacg gcatgggcat
     3661 tcgtgacaac tcacgcgggt acgactacga acgttaaaag gggactgtat ggcgagtaca
     3721 gaagaggggt tgctcacgct cttgttgtcc gttgagcatt tgaaaaacac tgcgatagaa
     3781 caacagaaag cggttgcaga cacgctggcg gtcgcaaggg aaacactgcg ggggtatcgg
     3841 gatatttctg acagtgttgt aaaacaggtt agcgatgaag tcagaaaagg ggtacaga
//
