LOCUS       NZ_CP046339             2579 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Burkholderia multivorans strain FDAARGOS_719 plasmid unnamed1,
            complete sequence.
ACCESSION   NZ_CP046339
VERSION     NZ_CP046339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056434
            Assembly: GCF_009730035.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia multivorans
  ORGANISM  Burkholderia multivorans
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Coverage                   :: 364.21x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 02:45:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,942
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,786
            CDSs (with protein)               :: 5,786
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 48 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 18 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Burkholderia multivorans"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_719"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_719"
                     /db_xref="taxon:87883"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: MN"
                     /collected_by="Mayo Clinic"
     gene            complement(join(2553..2579,1..87))
                     /locus_tag="FOC27_RS30475"
     CDS             complement(join(2553..2579,1..87))
                     /locus_tag="FOC27_RS30475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032667588.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDLSPLAAATGN"
                     /name="hypothetical protein"
     gene            787..1023
                     /locus_tag="FOC27_RS29820"
                     /old_locus_tag="FOC27_29815"
     CDS             787..1023
                     /locus_tag="FOC27_RS29820"
                     /old_locus_tag="FOC27_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1405..2049)
                     /locus_tag="FOC27_RS29825"
                     /old_locus_tag="FOC27_29820"
     CDS             complement(1405..2049)
                     /locus_tag="FOC27_RS29825"
                     /old_locus_tag="FOC27_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2143..>2298)
                     /locus_tag="FOC27_RS29830"
                     /old_locus_tag="FOC27_29825"
                     /pseudo=""
     CDS             complement(2143..>2298)
                     /locus_tag="FOC27_RS29830"
                     /old_locus_tag="FOC27_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1409..2049)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          449..622
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2..476)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.423"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cgataagtcg tgtctttccg ggttggactc aagatgatag ttaccggata aggcgcagca
       61 gtcggactga acggggggtt cgtgcataca gtccatccag gagcgaactg ccttcgcgga
      121 actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
      181 accgaaaggc aggaatgcgg gggagcacga gggagccacc agggggaaac gcctggtatc
      241 tttaagccgc atcgggtttc gccaccactg atttgagcgt cagatttcat gatgtttgtc
      301 aggggggcgg agcctatgga aaaacggctt cgccgcggcc ttatctcttc cctgttaagc
      361 tccttcctgg tatctcccag gaaatttccg ccccgcctgt aagccggcac cgctcgccgc
      421 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaatatat cctgtactac
      481 atatcctgct gacgcgctgg tgccgccttt tttctcctgc tacatgaagc acttcactga
      541 tttccacatc cgtgccaaca tagtcagcca gtatacactc cgctagcgct acgtgactgg
      601 ttcagggctg cgccccgaaa ccggctaaaa gctaatgacg cgcctgcggc ttgtccaacg
      661 cctcacggca ccggattaaa atccgctccc gttcaccgtt tctgactgcc tttcaccgtc
      721 gctggcgctt catcgtttct actgtgtctg gcagactgtg ctgatttatt ctgactggag
      781 aacgttatgg ccagactgac aaacttgacg cctgcagaga aaaaatttct tgatgatgct
      841 gttgccgcag ctgaaagagc gtcaggtaaa aagctaaatc agcctaaccg gcacatcgtc
      901 ctgaacagag cgcgtgcgca aattgagttg cagcgttacg ccgaccgcca gcgtgcatta
      961 cgtgaggatg agcggcagca gtccgatttt gcctggtcac gtccgcgcgc gccgcgccgc
     1021 tgattttggc ttttggcggt ttagtggtgc tgacgctgcg aaatcacctg ccattcacct
     1081 gcgtgcatag ccatgcagtc gcgcagaaat catcaggggc gtttttccgg gatactggtg
     1141 aggaaaggtg ctggaaacgg ccctgatgca gcaggattgc gctgagggcg cctgcggggg
     1201 tgcgggcaag ggatgttatg tttaatcggt ctgaccgtca ggtacaaaaa aaagccctta
     1261 gggggctttt tttttgctat gagttagcca tattttcgtg cgaatttttc atcggaatcg
     1321 catagataaa taataaactc aataaacgcg atgatcgcgg gtaaaagtaa atcgtcaaac
     1381 gcatctcccg gtgtaaacgc tgaactaacc aatcacagcg atgccaagcc gctccatgag
     1441 caacgatgcc tggtagctgt ctaacttaac gccttgtaaa tcaacacccc gaatatctaa
     1501 gtcacctaac tccgaattgg tcagatcgca atgtgtgaag tttgctgctc gccagtcgaa
     1561 agtcgaaaac tcgccgccgg agagatctga accactgaac gtcgcaccca gtacctgagt
     1621 ccccatccag cggttttccc acagctcaca cttttccaac acgactttcg aaaaattggc
     1681 gtagcttaga ttagtattag tgatatatgc gctgcaaaac caggtgcgcg tggtgatcat
     1741 attcataaag cttgcaccgc ggaaatctgc gccttgtgcg cggcagtggc gaatttcaat
     1801 gcccaaggca ctgacgttgc ggaaatctgc cattgataaa tcacagcttt tgaaaatggc
     1861 atctttcagc attgcgcgac taaaattgca ccctttctga ctttcgcgat catagaactg
     1921 gcagccgata aattcagtgc cgctcaggtc ggcacctgaa aaatcgcagt taaaaaatgt
     1981 actattttca actttctcac cggtgaagcg atttctgtca attttttcgc caactaatgc
     2041 cagagtcata tctatgcctg tgtttttata cagtaatgac gccatggtaa accttatgac
     2101 gttatgcgtc aaacccgcca gtatgacacc tgcaaatgcg tattagatct ggtgtttctt
     2161 cagcaaggca cgtagctgat gataagtcag acccagtaat tcagcggctt ttttctggtt
     2221 aaatttagcc tgctgtaagc tggtttgtag aaagtctttc tcttgctgct gctggaattc
     2281 acgcagatcc agcgttataa cgatgtgtat cctatgtgct cggattggat agatttccat
     2341 tacggtgaag gatcttcttg agatcctttc tgtggcgtcg taatctcttg ctctgtaaac
     2401 gaaaaaaccg ccctgcaagg cggtttttcg aaggttctca gagcagcaac tctttgaacc
     2461 gtggtaactg gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac
     2521 aggcgcataa cttcaagact aactcctcta aatcagttac cagtggctgc tgccagtgg
//
