LOCUS       NZ_CP046297             3065 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter lwoffii strain FDAARGOS_552 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP046297
VERSION     NZ_CP046297.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163245
            Assembly: GCF_009730095.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Patel,R., Rucinski,S., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3
            Genome Coverage                   :: 1151.80x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:29:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,407
            CDSs (total)                      :: 3,296
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 111
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 52 of 160
            Pseudo Genes (incomplete)         :: 111 of 160
            Pseudo Genes (internal stop)      :: 32 of 160
            Pseudo Genes (multiple problems)  :: 33 of 160
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046297.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_552"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_552"
                     /db_xref="taxon:28090"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MN"
                     /collected_by="Mayo Clinic"
     gene            join(2370..3065,1..243)
                     /locus_tag="FOB21_RS16990"
                     /old_locus_tag="FOB21_16990"
     CDS             join(2370..3065,1..243)
                     /locus_tag="FOB21_RS16990"
                     /old_locus_tag="FOB21_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080187.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_005080187.1"
                     /translation="MDLDVKKHYPKDWIVLQNRVVECFRGMSLDEKRLFIMATPLARTT
                     KISSNDPIFISSSDFSKECGIDLSTAYTALELASERLFTRFFGYTNAEGDRVKMRWLNK
                     VIYKAGQGGSELYFTDEVLLLLREFDALNPYTKYKKEVVLRLKKDYSLDFYHLAKKHQT
                     MGGFQISLDELFQQLGLPESYQDLSNLKKRVIKPSLDEITANTDIDLSYENVKRGRSVV
                     GFKFTVKEKPKPKVIETGRDPNTPDLFHKMTESQLDTFSSKLSELPEVQKMAHAGEDMK
                     PFIARIRSMLKDSEKQKTLLPHLAKLGFKSK"
                     /name="replication initiation protein"
     gene            240..431
                     /locus_tag="FOB21_RS16995"
                     /old_locus_tag="FOB21_16995"
     CDS             240..431
                     /locus_tag="FOB21_RS16995"
                     /old_locus_tag="FOB21_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005080189.1"
                     /translation="MMFEKKITQFEREVLLQEKIEQLENELKEFSDLQKKAYSERLQKS
                     IVGLENRIYRIKKMLYTT"
                     /name="hypothetical protein"
     gene            583..1716
                     /gene="mobV"
                     /locus_tag="FOB21_RS17000"
                     /old_locus_tag="FOB21_17000"
     CDS             583..1716
                     /gene="mobV"
                     /locus_tag="FOB21_RS17000"
                     /old_locus_tag="FOB21_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_005080191.1"
                     /translation="MSFAILRIQKLKSFADVGGSLSHNYRNRETLNADDARTHLNEHAL
                     DTNEKCMTAIRDRIPEKRRKDAVLCIEHLITASPEWDGWGTEKETAFFEQSKKWLENKY
                     GKNNVVSTTIHRDETTPHLVAYVVPVDEETGRLNAKKYIGGSRHTLSQMQTDFAVEVKD
                     LGLDRGIQGSKAKHTSIQEYYEKLNNYENEPGIEKGLTYEVPEPEFFESKNAYGERVAN
                     AMIDQIFDQIAPRFDRANALASQTEKLKKELSDTKKTLDEVQQRAKPYLDIINEYNHPN
                     LEKEFNKQVAKLKDNFDSALEHHRFLKRQEEQERFNQQRELRNQLHLEHEQKKQLAEQE
                     KQEKERLALLRRQELEKQRKNEPKKPDNANDNDYSPS"
                     /name="mobV"
     Typing          2371..3065
                     /name="rep_cluster_1223"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.241"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          554..1713
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcccaaag ttatagaaac aggacgagat ccaaatacac ctgacctatt ccacaaaatg
       61 acagaatctc aacttgatac gtttagcagt aaactttcag aactccctga agtgcaaaag
      121 atggcacatg caggcgaaga tatgaaacca tttattgctc gtattcgttc gatgctcaaa
      181 gactctgaaa aacaaaaaac gttgcttccc catctagcaa aattaggatt taaatcgaaa
      241 tgatgtttga gaaaaaaatc actcagtttg aaagggaagt tttgctacaa gaaaaaattg
      301 agcagctcga aaacgagctt aaagaatttt ctgatcttca aaaaaaagca tatagcgaac
      361 ggcttcaaaa aagtatcgtg ggtttagaaa atagaatcta tcgaatcaag aaaatgcttt
      421 atacaacctg aaaggttgtc aggggttaag gggaggattc cccttactca tgaactttca
      481 atgtaccttt aggtgctttg aaagtggagt gagtccactt cgctatcgca aagctcaaaa
      541 agctctgcta tcgtgtgttt tttgcggtgt cggatttcgc ccatgtcatt tgctattttg
      601 cgtattcaga aacttaaatc atttgccgat gttggaggca gtctttcgca taattatcgc
      661 aatcgagaaa cgttaaatgc cgatgatgct cgtacacatt tgaatgaaca tgccttggat
      721 accaacgaaa aatgtatgac cgctattcgt gatcgtattc ctgaaaaaag acgtaaagat
      781 gcagttttgt gtattgaaca tttgattaca gcgagtccgg agtgggatgg ctggggaact
      841 gaaaaagaaa ccgcattttt tgagcagtcg aaaaaatggc ttgaaaataa atatggtaaa
      901 aataatgtcg tcagcaccac cattcaccgt gatgaaacaa cccctcacct tgtcgcctac
      961 gttgttccag tggacgaaga aacggggcga ttgaatgcta aaaaatatat cggtggatct
     1021 cgacatacac tttcacaaat gcagactgat tttgcagttg aagtaaaaga tttaggacta
     1081 gatcgaggga tccaagggag caaggcaaaa catacgtcta ttcaagaata ttatgaaaaa
     1141 ttgaacaatt acgagaatga accaggcatt gaaaaaggac tcacctatga agtgcctgaa
     1201 cctgagtttt ttgaatctaa aaatgcctac ggtgagagag tcgcaaacgc tatgattgat
     1261 cagatatttg atcaaattgc gccacgattt gaccgagcga atgcactggc tagtcaaacg
     1321 gaaaaattaa aaaaagaact gtctgatacc aaaaaaacac tcgatgaggt tcagcaacga
     1381 gcaaagccat atttagacat aatcaacgaa tataatcatc caaatcttga gaaagaattt
     1441 aataagcaag ttgctaaatt aaaagataat tttgattcag cacttgaaca tcatagattc
     1501 ctaaaaagac aggaagaaca agaaagattt aaccaacaac gtgaacttcg caatcaatta
     1561 cacttagagc acgaacaaaa aaaacaactg gctgagcaag aaaagcagga aaaagaacgt
     1621 ttggcacttt taagacgtca agaattagaa aaacagcgaa aaaatgagcc taaaaaacct
     1681 gataacgcca atgataacga ctactcaccc tcataacacc catttaaacg caaaaaaatg
     1741 gggtttttag ccatgtttgg tgttttatgc atgaaagtat agttgagata ataaaaacct
     1801 cttagaacgc aaattagagc gttttagcga gtgtttacga gcgacacaat gaaaattcgc
     1861 ttattccccc ctctggaaaa ccgcttttgc tttttaaaac agatgaatcg cagatgtagc
     1921 aatgctttca acgctatatc tatacgttta tcgacccaaa gagttagcga gtgtctatga
     1981 gcggagtatt gatgcttttg ctcttaaaaa agcatgagca tagcgaatgc attagtaacg
     2041 cttttgcttt tgcttttgct cttgaaaaga cacgagcgaa gcgagtgcca tagcctttga
     2101 ttttgccttt gattctcttt tttagctttt aaaaagcttt taaagacttt tcgcccctct
     2161 acagaccttg ctagacatag ctttcagact ttaaaaaagt acaaatgcca agtattaaag
     2221 tacaaacgcc aagttaaaag cacttaaaaa agtacaaacg ccaagttaaa aaagtacaaa
     2281 cgccaagtat taaagtacac aattaaactt tgaactttta ttggttaaaa ggtattgtct
     2341 taaacgatgt actttatttg gaaaatacta tggatttgga tgttaaaaaa cactatccca
     2401 aagactggat agttttacaa aatagagtgg ttgaatgctt tagaggtatg tcccttgatg
     2461 aaaagcgttt atttatcatg gcaacccctt tggcaagaac cacaaaaata tcaagcaatg
     2521 atcctatttt tatatcttcg agtgactttt caaaggagtg tggcattgac ttatcaacgg
     2581 cttatacagc tttagaattg gcatctgaac gcctatttac tcggttcttt ggttacacca
     2641 atgctgaggg tgacagggta aaaatgcgtt ggctaaacaa ggtgatttat aaggcagggc
     2701 aaggcggttc agaactatat tttacagatg aagtacttct attacttaga gagtttgacg
     2761 ccctaaaccc ttataccaag tataaaaaag aggtcgtttt acggcttaaa aaagactact
     2821 cgctagactt ctatcacttg gctaagaaac accaaacaat gggcggtttt caaatcagcc
     2881 tagacgaatt atttcagcag ttgggcttgc ctgaatccta tcaagaccta agcaacctaa
     2941 aaaaacgtgt aattaagcca tcactggacg agatcacagc caacaccgac atagacctaa
     3001 gctatgagaa tgtgaaacgt gggcgttccg tggttggctt taagttcact gtcaaagaaa
     3061 aacca
//
