LOCUS       NZ_CP046287             9180 bp    DNA     circular CON 09-JUL-2023
DEFINITION  Shigella sonnei strain FDAARGOS_715 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP046287
VERSION     NZ_CP046287.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056430
            Assembly: GCF_009729995.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Fashae,K., Tallon,L., Sadzewicz,L., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y. and
            Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Fashae,K., Tallon,L., Sadzewicz,L., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y. and
            Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3
            Genome Coverage                   :: 521.02x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/09/2023 03:33:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,196
            CDSs (total)                      :: 5,069
            Genes (coding)                    :: 4,349
            CDSs (with protein)               :: 4,349
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 720
            CDSs (without protein)            :: 720
            Pseudo Genes (ambiguous residues) :: 0 of 720
            Pseudo Genes (frameshifted)       :: 217 of 720
            Pseudo Genes (incomplete)         :: 505 of 720
            Pseudo Genes (internal stop)      :: 128 of 720
            Pseudo Genes (multiple problems)  :: 123 of 720
            Pseudo Genes (short protein)      :: 3 of 720
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_715"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_715"
                     /db_xref="taxon:624"
                     /plasmid="unnamed2"
                     /geo_loc_name="Nigeria: Ibadan"
                     /collected_by="University of Ibadan"
     gene            join(9146..9180,1..1165)
                     /gene="tet(A)"
                     /locus_tag="FOC23_RS25425"
                     /old_locus_tag="FOC23_25390"
     ARG             3..1162
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="96.99"
     gene            complement(1197..>1541)
                     /locus_tag="FOC23_RS25430"
                     /old_locus_tag="FOC23_25395"
                     /pseudo=""
     CDS             complement(1197..>1541)
                     /locus_tag="FOC23_RS25430"
                     /old_locus_tag="FOC23_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /name="EamA family transporter"
     gene            complement(<1538..1735)
                     /locus_tag="FOC23_RS25435"
                     /old_locus_tag="FOC23_25400"
                     /pseudo=""
     CDS             complement(<1538..1735)
                     /locus_tag="FOC23_RS25435"
                     /old_locus_tag="FOC23_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1779..2168)
                     /locus_tag="FOC23_RS25440"
                     /old_locus_tag="FOC23_25405"
     CDS             complement(1779..2168)
                     /locus_tag="FOC23_RS25440"
                     /old_locus_tag="FOC23_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2386..2826
                     /locus_tag="FOC23_RS25445"
                     /old_locus_tag="FOC23_25410"
     CDS             2386..2826
                     /locus_tag="FOC23_RS25445"
                     /old_locus_tag="FOC23_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3832..3981)
                     /locus_tag="FOC23_RS26220"
     CDS             complement(3832..3981)
                     /locus_tag="FOC23_RS26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5170..5985
                     /gene="sul2"
                     /locus_tag="FOC23_RS25455"
                     /old_locus_tag="FOC23_25420"
     ARG             5171..5982
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6046..>6573
                     /locus_tag="FOC23_RS25460"
                     /old_locus_tag="FOC23_25425"
                     /pseudo=""
     CDS             6046..>6573
                     /locus_tag="FOC23_RS25460"
                     /old_locus_tag="FOC23_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            6594..7067
                     /gene="dfrA14"
                     /locus_tag="FOC23_RS25465"
                     /old_locus_tag="FOC23_25430"
     ARG             6595..7064
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <7142..7417
                     /locus_tag="FOC23_RS25470"
                     /old_locus_tag="FOC23_25435"
                     /pseudo=""
     CDS             <7142..7417
                     /locus_tag="FOC23_RS25470"
                     /old_locus_tag="FOC23_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            7417..>7935
                     /locus_tag="FOC23_RS25475"
                     /old_locus_tag="FOC23_25440"
                     /pseudo=""
     CDS             7417..>7935
                     /locus_tag="FOC23_RS25475"
                     /old_locus_tag="FOC23_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]; GO:0019748 - secondary metabolic process [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family protein"
                     /name="aminoglycoside phosphotransferase family protein"
     gene            8116..8358
                     /locus_tag="FOC23_RS25480"
                     /old_locus_tag="FOC23_25445"
     CDS             8116..8358
                     /locus_tag="FOC23_RS25480"
                     /old_locus_tag="FOC23_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(8390..9040)
                     /gene="tetR(A)"
                     /locus_tag="FOC23_RS25485"
                     /old_locus_tag="FOC23_25450"
     CDS             complement(8390..9040)
                     /gene="tetR(A)"
                     /locus_tag="FOC23_RS25485"
                     /old_locus_tag="FOC23_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             7173..7417
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     ARG             6047..6588
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     Typing          complement(4017..4190)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4114..4719
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
       61 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
      121 tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
      181 gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac
      241 ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac
      301 tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca
      361 cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg
      421 gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct
      481 caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt
      541 acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt
      601 cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct
      661 ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct
      721 tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc
      781 ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat
      841 cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc
      901 gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg
      961 tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc
     1021 cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat
     1081 tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg
     1141 cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa
     1201 ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt tggaacgttg gcccagccag
     1261 atactcccga tcacgagcag gacgccgatg atttgaagcg cactcagcgt ctgatccaag
     1321 aacaaccatc ctagcaacac ggcggtcccc gggctgagaa agcccagtaa ggaaacaact
     1381 gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc
     1441 aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca
     1501 aacactaaag ctactggaac gagcagaagt cctccggccg cccgcattgg agaaattctt
     1561 caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga
     1621 atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct
     1681 ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc
     1741 gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc
     1801 tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccactctt
     1861 acattgttcg cagtatctag gtacatgtta ttgcgtgcac cttcatgatg tataatgtta
     1921 ttaatgacag cagaaagatc gacagaatta atattcttcg tacctgggac agttatcggg
     1981 tgtcggccag tgatgagacc aaaggatgca gtataagatg aaccactact attatcatgg
     2041 ccaccattac caccaccgtt aaaactacct cccgaacttc cagagccgct atgaccatca
     2101 ttgtaggggt catttggtcc catgtgacca aaaccaccga cagtaccgct atttaactct
     2161 gtactcataa aattatcctc tttgactttt aaaaacagca aaaactgtat ttataacaat
     2221 gtgtttatat acagtattcg aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt
     2281 ttgtgtattt ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg
     2341 ttctgctgtt acaatacggt acacacagga gacactgtca cttttatggc atggcacaca
     2401 gttcaagaga cactgaaatt aacaggaaag tcgcgctcgc aattatatcg tgacatggct
     2461 aaaggccttg tcagctatcg cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa
     2521 ctgattcggg cctatggcga gctgcgcact tctgagacac caaaaaggca caaggaggga
     2581 cactccaaat ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa
     2641 ctggacgctc tgcagcagac tgttacccta atgcttgaag acaagatggc gcgggaagca
     2701 gaaaaccgcc tgcgaaatga ggagagggaa caactacagg ccgaaattag ccgtctggct
     2761 gacgcgctgg cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg ctccggttca
     2821 gagtaacagt gcaggtgact ctgatttacc ataacatctg ttatcagcac cgcctgagag
     2881 gccctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc
     2941 gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcaca agtgcatagc
     3001 gcggaaattc acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa
     3061 aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa
     3121 aacccgctgt cagcacgttc aggggtttga ggcccaaccg tacgagatcg tacgctaagt
     3181 tcattttttg agcagaagag gtgagtaaag aaaacgaggg ttttgtttac tcttcccgtc
     3241 aatgccagta gccatgcggt ttcagggact ttcagtgtta ataaactcgg taatcaaatc
     3301 aacgtaaacc tgcctaacaa ctccacgact ttattgactt aaaatcatat agttagcttc
     3361 ttagcgtacg tttccgctcc attgggcttc aaaccccgtt caggaagggg gtgatgtaca
     3421 aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa gtgatgtata
     3481 agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt caggaagaat
     3541 aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc acgatctccc
     3601 cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa atggtcatac
     3661 acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa aaaggaatat
     3721 ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat gaaaataaag
     3781 aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa gctataacgc
     3841 ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt cagtaacctg
     3901 cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg aatccagttg
     3961 ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt tagcgggttt
     4021 cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg cttactatgt
     4081 tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa ggcagcaccg
     4141 gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac tgactcgcta
     4201 cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg gaaatttcct
     4261 ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa gccgtttttc
     4321 cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca gtggtggcga
     4381 aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct
     4441 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt
     4501 ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa
     4561 ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg
     4621 gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat
     4681 agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt
     4741 aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc
     4801 gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta cgattcattg
     4861 aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac
     4921 cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt
     4981 gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat
     5041 ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct
     5101 ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat tattattata
     5161 gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt
     5221 ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg
     5281 atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg
     5341 cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca
     5401 gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg
     5461 cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa
     5521 ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat
     5581 cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc
     5641 atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg
     5701 gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa
     5761 ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc
     5821 gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc
     5881 gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg
     5941 ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat
     6001 atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat
     6061 attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat
     6121 tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc
     6181 ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc
     6241 cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt
     6301 ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg
     6361 gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg
     6421 ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac
     6481 ttcttaccgg acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga
     6541 gagctaccgg tgcggctcga ccaagagcgc accgttaacc caggatgaga accttgaaag
     6601 tatcattgat agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct
     6661 ggtccgcgaa aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg
     6721 tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta
     6781 cccgctcagg ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg
     6841 ccatggacag gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt
     6901 accgagaaac attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag
     6961 agggggatgt tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact
     7021 ttacttcaaa cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa
     7081 tcgacggcaa aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga
     7141 cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac
     7201 tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga
     7261 tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg
     7321 cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc
     7381 cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct
     7441 gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg
     7501 aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac
     7561 attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc
     7621 cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc
     7681 tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg
     7741 gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat
     7801 cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac
     7861 tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg
     7921 ctacatggcg agctgctgca attcctgtcg ccgctgggct gggagcacat caacctaacc
     7981 ggcgattacg tctggcggca gagccgcaga ctggaagacg ggaagtttcg gcccttacgg
     8041 atgcccggaa aaccttagcg tacgattttt tccgaattct gcgggctccc ctatctcatc
     8101 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
     8161 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
     8221 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
     8281 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
     8341 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
     8401 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
     8461 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
     8521 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
     8581 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
     8641 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
     8701 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
     8761 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
     8821 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
     8881 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
     8941 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
     9001 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
     9061 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca
     9121 gcggaggctg gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag
//
