LOCUS       NZ_CP046249             5350 bp    DNA     circular CON 17-APR-2024
DEFINITION  Enterococcus faecalis strain RF_1_1_25_1 plasmid pRFP2, complete
            sequence.
ACCESSION   NZ_CP046249
VERSION     NZ_CP046249.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13336268
            Assembly: GCF_022810805.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Poosakkannu,A. and Novakova,E.
  TITLE     Genome of Enterococcus faecalis isolate from Rhodnius prolixus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Poosakkannu,A. and Novakova,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2019) Parasitology, University of South Bohemia
            Ceske Budejovice, Branisovska, Ceske Budejovice 370 05, Czech
            Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2019
            Assembly Method                   :: Flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 1000.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022810805.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 09:58:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,744
            CDSs (total)                      :: 2,668
            Genes (coding)                    :: 2,627
            CDSs (with protein)               :: 2,627
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 25 of 41
            Pseudo Genes (incomplete)         :: 14 of 41
            Pseudo Genes (internal stop)      :: 7 of 41
            Pseudo Genes (multiple problems)  :: 4 of 41
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046249.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="RF_1_1_25_1"
                     /isolation_source="feces"
                     /host="Rhodnius prolixus"
                     /db_xref="taxon:1351"
                     /plasmid="pRFP2"
                     /geo_loc_name="Czech Republic: Ceske Budejovice"
                     /collection_date="2019-07-10"
     gene            complement(192..392)
                     /locus_tag="GLO19_RS13625"
                     /old_locus_tag="GLO19_14065"
     CDS             complement(192..392)
                     /locus_tag="GLO19_RS13625"
                     /old_locus_tag="GLO19_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(481..2160)
                     /gene="mobV"
                     /locus_tag="GLO19_RS13630"
                     /old_locus_tag="GLO19_14070"
     CDS             complement(481..2160)
                     /gene="mobV"
                     /locus_tag="GLO19_RS13630"
                     /old_locus_tag="GLO19_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_243513422.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDRITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            complement(2938..3234)
                     /locus_tag="GLO19_RS13635"
                     /old_locus_tag="GLO19_14075"
     CDS             complement(2938..3234)
                     /locus_tag="GLO19_RS13635"
                     /old_locus_tag="GLO19_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(3361..4083)
                     /locus_tag="GLO19_RS13640"
                     /old_locus_tag="GLO19_14080"
     CDS             complement(3361..4083)
                     /locus_tag="GLO19_RS13640"
                     /old_locus_tag="GLO19_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            4708..4794
                     /locus_tag="GLO19_RS13645"
                     /old_locus_tag="GLO19_14085"
     CDS             4708..4794
                     /locus_tag="GLO19_RS13645"
                     /old_locus_tag="GLO19_14085"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          complement(3362..4083)
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(485..2235)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.829"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccattgtact tcccaaatat acttctgaag gaattaaaaa aaataataaa attccgtaaa
       61 aaataattat caatatgggc aaatcaatat taactaaaaa agaagttata tagcttaaaa
      121 ctaaaggaat agtaatataa aatatatttt ttattagata ttttttcatg aaataacctc
      181 aaatttagca actattttgt ccatcttgta tgcattggga tacaaaatcc accggcttta
      241 tgatatcctc ctgcagtcca cccgcctttt ttccaataaa atcctggctg acactctata
      301 gaatcccact ctctagcact tgcttctgta gttgaactta aagcaacaga gataaaagac
      361 acccctaaaa ttagaactaa caactttttc ataatgctta cctcactttt ttttaatttt
      421 tattaatata ttttaattta actgttttta tgtgtcaata atttatcaat taaattattt
      481 ttaacgagat aaacctcgtc cttttgacat atctcttgaa tattttttac tctgttgcca
      541 agccttttta tctgtcgact gttcaaacgt aaaagctttc ttctctagca cttgttcttc
      601 agacagttgt aaagtattcg ttcgagaacg tccattagcc atatattcgc cgacaaactc
      661 ataacaccct gtttctctgt tgtaaaacgg ggcttttagg gggtagaaat gtgggtttaa
      721 ggacattgtt accccttttg accccgtatc tggtggtaaa aacaatgtcc ctttttcgtc
      781 aatgaggtaa gttctatcct tggaatatcg ctctagggat ttttttaatt taggcgcgtt
      841 ctgaaggtac ttcactcctt gctgaatcgt aaataaaatg gtttctttag catgacctag
      901 tctttcgtga aggtaagtag ttaacccgtc aaactttaat tttatttctt gatatttttc
      961 atttagttca gaaaagtcgt aggttaagtc agtaattcta tcttttaacc gcttattctc
     1021 tgtttctaac acagaaactg tttgtgttgt tgtatcaagt tcttgtttga tttgttcgct
     1081 ctggtcatag tttagttgtt tttcttcaaa cgctgttaat tgttcttttt tcagggttaa
     1141 ttcataggct aattccgtta tttgtaattc ttgttcatga tttacttgtg tcaattcttt
     1201 atttttctgt gccaagttat tgttttcgat tttaaggtgc ttattttgcg ttctaagcgt
     1261 ctcgaaatct ttcgtaatac tttttatcgc cgaaaagtca gaagcgctta aaacgtattt
     1321 taaaccgttt aaactaaatt tcttttcttt gacatttgct aaatcaggta aaacggttgt
     1381 ttgtagagag ttaatgattt cgtttcgctt cgtttccagt tggttcattg cttggacttt
     1441 gccctttact tcttcgttct tctcttcaag aaggcggtca atacggaaga ttgcctgttc
     1501 ataagaatgg agttctctct ctttttctgc cagaatttgt tcctgattag cgagttcctg
     1561 cttagaaact tgtagctcct tttgaacgtc tttgtattct ttgacagata aatgcttttg
     1621 cggactccca cggagtccac gctcaatagc atagcctcgt tcttgcaaat aggttggcaa
     1681 ttcttcttga atctctaaca aattttgccg actaaacacc tgtttactgc tcaatcgccc
     1741 ttcgtacata ggcacaattc ctaagtgcat atggggcgtg gtttcgtcta aatgtaccgt
     1801 ggcataggcg acattttgtc gaccgcagcg atcagagaaa tatgcgacaa catcggtaaa
     1861 aaatgcttgt gtgtctgtat tctcttgaaa aaaggcggta tcactagtga ttatccattc
     1921 gttcacaagg actgcgtctt ttctaaccgc tcgtttactg attcgttggc tctcaatgat
     1981 ttgtttgatt ttctcccgat aattgattga accaggattg actaagtcat aattcaagtg
     2041 agatttctca atatcaatat caggattgga atgattattg gtttctcgtt gattatgtcg
     2101 ttggatacca cttaaatttc cgtctttcat tttttccata cgtgcaacaa tttttgacat
     2161 gagcgctcct cccccttttt tttcttgtgt cgttcggtgt ctttggattt cggcagtact
     2221 tccaagtaaa gtataacgca ctatacttta cttcgtaaag gtgcgctctg ccgagggctg
     2281 tcggctagta ctcgaaaggt cagcctttag gcttcccaat ctcgcactta gccgattgcc
     2341 tacggtcaaa ctttccattt ctgctaaggc agaaactcca aatttccctc cgtcagaaaa
     2401 ccgtttaaat aacgactgcc tgaaattagt gaacgtgcta gctttctgct agccggtgct
     2461 agtaatgtgc taatttggtg ctaaaaacct gctagtcact gctaggaatc tgctagtttg
     2521 ggtgctagaa acctgctatt gagtgctaaa aatgtgctag tttacctgaa agtacctgct
     2581 agtaaagtgc tagcgaagaa cgaaagtgta ccccacggga aagccttgca ctgcaaggcg
     2641 gggtaccttg tgtgccccac agttcccctt atgctcttcc gaaacacagg aagaaattaa
     2701 gcgaatttca acagtaaaag caaaaaataa cagcagtggg attgggggaa ccccaaaaat
     2761 ttgaacgact cctaccagta tttttggtag gggtcattca aaatatcgca acatggtacc
     2821 atgttgctct gctcgcaaaa agaaagccta ccccaaaatg gaagtaggct aatcttgata
     2881 tttttcgagt gttatcccaa atagtcaaca tttttttata acaagcaagg gtagttttta
     2941 ttcttcttcg tctagagatt gaccgtcaat cttcacaatc ccacaggaaa tatcttcttc
     3001 tagcgtaact ttgtaggcta ccgtatcaat aagtagaatg tatgaccgac ttagataatt
     3061 aaacattctt agcgtttcgt ctcgaactac agtatctaat cgtgataagt caagtggctc
     3121 caaatcaagc aatagcgctt ctagttcact ctcaaactta tctagtcgct ccagtacagc
     3181 ctgtgtgact tcgtactcct cttttgtcat gttgtatcct gtcattaatc ccataagtag
     3241 gacctccaaa ataatttatt ttagaagtcc tcacgcaaat atgctaaaat aatctagcag
     3301 tccggaagat tgcgtgagga acttgctaac ttgcttaatg tgagggggca agttcttttt
     3361 ttattctccg tttacccaat cgtgcatggt tacgcttggt ttgttggttt tcagaccagt
     3421 aaacgtaaat tctagtttgg caatcttatt gccttttttg gctttgattt ttgttacttc
     3481 taaattctca aaatagttat ggagttcttt cattgctggt tttaaaacct tttggtcaat
     3541 gttacccatt tgataatatt cgggtatgtc taacaattct ctgaagtctt caatactgac
     3601 tacataatac cctgttgaac gatattgcat taacaaacga aacaatgttt tagcatatgt
     3661 tgagcgtatt gaagtaaaag cggacagctc aaacctacta aactctgttg ttaaaccatt
     3721 tattatatag tctaaatctt gattaactcg aacttcaaca aacttttgtt ttttgtcgat
     3781 tttaaatcca ttgaataaaa caaaatactc tatttcgtca tcgttttctg tacgataaga
     3841 aaggttcagc atatctttat acaacttttc taagtctgct acaaaggctt ttgtagcagt
     3901 cattttgtag tcactcaatt cctttaattc ctcgaaggag aaacgaatat tctctactcc
     3961 tttatctttc attcgtgcgc ataacgcaaa gaacagatcc atttgttttg cattaaagtt
     4021 tttaagtggc acaagattta actcattttg atatttaaca gctaattccg atttatcact
     4081 cattcctttc gccccctcac aattcgaata ttaaaacaaa caaccaactt tgtcaacagt
     4141 tggttgtttg ttttaatatt atgaaaataa aacagataaa aagttgttgg tagtcagata
     4201 aaaagttgtt ggtagtcaga taaaaagttg ttggtagtca gataaaaagt tgttggtacc
     4261 atctgctctt ccttagagcc acgtggcaca cagcccgcct aaaaaggttt taaaaatata
     4321 tataaaatat atataaaaga aacgcgtgcg cgcgcggaaa tttcccgccc aaaattaacc
     4381 tcttttttct acaaacaggt ccgcgatatt tacaaaactg gcactaaaaa atcaaaaaag
     4441 gagtgagcga aaatcccgct cacattttgc ttgcagcgtt cacttcgttc actcgctaaa
     4501 atcaaaatca ataccgttga caaggtaaat catttggtta ggctctattt gcttgtctga
     4561 agatctttaa gacatgttta gttgttttag ggtatcttgg ttaggattgc gccttgcagg
     4621 cggtcagggc aagccctaac aacccccaca agaaattctt cttgtgatat acttgttttg
     4681 gcaactgccc cgaaaggggg tgtaaacatg attgaaagct taatttcgtt gattatcgcc
     4741 ccactttttg tgggtttagt acttttgcta gttgatcgtt ggctagacga ttagcagata
     4801 atctatgcag ttgcttttct agctccaacg ctgaaatcgc aacagtgtaa aaaagccctc
     4861 ctactgagaa taggagggct ttttggtgta aacatgattt tgcttaattt cgcttcgcac
     4921 ttacacaata acattttttt tctgtatttt caaacatcag attagattac gcaaaacgtc
     4981 aattagagaa ataccatatc gaaatagtta gtattaaaca tagtaatact atgaggatag
     5041 acaatatttt actttttgga ctatcttcaa agcttttatt ttctggtctg taagtgggat
     5101 tcactacttt cgcgttgtaa tccattgtac ttcccaaata tacttctgaa ggaattaaaa
     5161 aaaataataa aattccgtaa aaaataatta tcaatatggg caaatcaata ttaactaaaa
     5221 aagaagttat atagcttaaa actaaaggaa tagtaatata aaatatattt tttattagat
     5281 attttttcat gaaataacct caaatttagc aactattttg tccatcttgt atgcattggg
     5341 atacaaaatc
//
