LOCUS       NZ_CP045830             2974 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain AUSMDU00014361 plasmid pAUSMDU00014361_03,
            complete sequence.
ACCESSION   NZ_CP045830
VERSION     NZ_CP045830.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11008224
            Assembly: GCF_009650035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Baines,S.L., Goncalves da Silva,A., Carter,G.P., Jennison,A.V.,
            Rathnayake,I.U., Graham,R.M.A., Sintchenko,V., Wang,Q.,
            Rockett,R.J., Timms,V.J., Martinez,E., Ballard,S., Tomita,T.,
            Isles,N., Horan,K.A., Pitchers,W., Stinear,T.P., Williamson,D.A.,
            Howden,B.P. and Seemann,T.
  TITLE     Complete microbial genomes for public health in Australia and
            Southwest Pacific
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Baines,S.L., Goncalves da Silva,A., Carter,G.P., Jennison,A.V.,
            Rathnayake,I.U., Graham,R.M.A., Sintchenko,V., Wang,Q.,
            Rockett,R.J., Timms,V.J., Martinez,E., Ballard,S., Tomita,T.,
            Isles,N., Horan,K.A., Pitchers,W., Stinear,T.P., Williamson,D.A.,
            Howden,B.P. and Seemann,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2019) University of Melbourne, Microbiological
            Diagnostics Unit, First Floor, Building 248, Parkville, Victoria
            3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-OCT-2019
            Assembly Method                   :: Unicycler v. 0.4.8b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009650035.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,898
            CDSs (total)                      :: 5,758
            Genes (coding)                    :: 5,379
            CDSs (with protein)               :: 5,379
            Genes (RNA)                       :: 140
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 379
            CDSs (without protein)            :: 379
            Pseudo Genes (ambiguous residues) :: 0 of 379
            Pseudo Genes (frameshifted)       :: 170 of 379
            Pseudo Genes (incomplete)         :: 214 of 379
            Pseudo Genes (internal stop)      :: 80 of 379
            Pseudo Genes (multiple problems)  :: 72 of 379
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045830.1.
            Bacteria and source DNA available from Bacteria and source DNA
            availalbe from Microbiological Diagnostic Unit Public Health
            Laboratory, Victoria, Australia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AUSMDU00014361"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pAUSMDU00014361_03"
                     /geo_loc_name="Australia"
                     /collection_date="2015"
     gene            1..195
                     /locus_tag="GIJ02_RS29075"
                     /old_locus_tag="GIJ02_29060"
     CDS             1..195
                     /locus_tag="GIJ02_RS29075"
                     /old_locus_tag="GIJ02_29060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001399823.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENSTGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1013..1537
                     /locus_tag="GIJ02_RS29080"
                     /old_locus_tag="GIJ02_29065"
     CDS             1013..1537
                     /locus_tag="GIJ02_RS29080"
                     /old_locus_tag="GIJ02_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001067571.1"
                     /translation="MNPDLLVIFERALEGITSVSVQKKTNDTYTLKSDHLNTYMLLTII
                     SGNNEDDKLKLKLSFVLSLPIDGVNKDILDGIIKAGFSLQENSPCKLIGITETDNELKV
                     ELVIFYTDYASVFSEADREDKYKESDFKLIVLSMLSELYSCSMGINNFISKTLLSALEN
                     IKKGTKDEEKR"
                     /name="hypothetical protein"
     gene            1521..1955
                     /locus_tag="GIJ02_RS29085"
                     /old_locus_tag="GIJ02_29070"
     CDS             1521..1955
                     /locus_tag="GIJ02_RS29085"
                     /old_locus_tag="GIJ02_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024242988.1"
                     /translation="MKKNDDMLCLVIKLLLSVFIAFIFAFKYKQELLSALSEQVYYQLL
                     VVVTIISGFLANPIGYFIYNIHVYLRRKISKEYNAEYLSEQVKLLSKTDLKNISEKHKE
                     MIESWGGRDPLSEFKIELVSAELERLRKSKNDQATQPTTE"
                     /name="hypothetical protein"
     gene            complement(2380..2703)
                     /gene="mobA"
                     /locus_tag="GIJ02_RS29090"
                     /old_locus_tag="GIJ02_29075"
     CDS             complement(2380..2703)
                     /gene="mobA"
                     /locus_tag="GIJ02_RS29090"
                     /old_locus_tag="GIJ02_29075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          complement(2713..2938)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.75"
                     /coverage="99.0"
                     /evalue="2.28e-73"
     Typing          239..758
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(2384..2703)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.25e-63"
ORIGIN
        1 atgaacaagc agcagcaaac tgtactcaac atggcgggat tcataaaaag ccagagcctg
       61 acactgctcg aaaaactgga cgcactcgat gccgacgaac aggccgccat gtgtgagaag
      121 ctgcacgaac tcgcggaaga actccagaac agcatacaga cacgctttga agcggaaaat
      181 agcaccgggg cataacggtg tatccggcat cagacaggat gcagaacggt atacctgtgc
      241 gtaaggagaa aatgatacat aaggcgctct tccgcttcct cgctcactga ctcgctacgc
      301 tcggccgttc ggctgcggcg agcggtatca gctcactcaa aaacggtaat gtggtgatcc
      361 acagaatcag gggataaagc caaaaggagc atgtgagcaa aaaacaagaa ccaggaaaag
      421 gccgcgccga tggcgttttt ccataggctc cgcccccctg acaagcatca caaaaatcga
      481 cgctcaagtc agaggtggcg aaccccgaca ggactataaa gataccaggc gtttccccct
      541 ggaagctccc tcgtgcgctc tcctgttccg accatgccgt ttaccggata cctgtccgcc
      601 tttctccctt caggaagcgt ggcgctttct catagctcac gctgttggta tctcggcccg
      661 gtgtagatcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgactac
      721 cacgttgttc cggtaactat caccttgatt ccaacccgaa acagacacga aaatcgccag
      781 tggcggtagc cattggtaaa caggatgtgc gagagatgta tcgagagttc ttgaagtggt
      841 taccttactc cggctacact gaaaggacag atttggtgac atgtctcgca caagactgtt
      901 acctcggttc aggaacttgc cggtttactg aaccttagaa aaaccacctt gccgggtggt
      961 tttttctttt ccgtaggtta aggcggcaaa acagcaacgt caaggtaaac aaatgaatcc
     1021 agacctatta gtgatttttg agcgagcttt agaaggcata acatcagtct cggtacaaaa
     1081 aaaaacaaat gacacatata ctttaaaatc cgaccatctc aacacataca tgttgctaac
     1141 aataataagt ggaaataacg aagatgataa gctgaaatta aagctatctt ttgttttatc
     1201 cttaccaata gatggggtga acaaagacat attagacggc ataataaagg ctggcttttc
     1261 tttacaagaa aactctcctt gcaaactaat aggcataaca gaaactgata atgaattgaa
     1321 ggtagagctt gttatatttt acactgatta cgcatctgta tttagcgagg ctgacagaga
     1381 agataaatat aaagaatcag atttcaaact aatagtgtta tcaatgcttt cagagctgta
     1441 ttcttgctca atgggaataa acaacttcat ttcaaaaacc ttgctcagcg ctcttgagaa
     1501 catcaaaaaa ggtacaaaag atgaagaaaa acgatgatat gctatgcctt gtaattaaac
     1561 tgctcttaag tgtttttatt gctttcattt ttgcattcaa atacaaacag gagctcctgt
     1621 cagctttatc tgagcaggta tactatcagt tactggtcgt tgtaaccata atttcaggct
     1681 ttttagccaa tccaattgga tattttattt acaatatcca tgtatatttg cgcagaaaaa
     1741 tatccaagga atacaatgct gagtacttat ctgagcaagt aaaattattg tcaaaaaccg
     1801 acttaaaaaa cataagcgaa aagcacaaag aaatgattga gtcttgggga gggcgagatc
     1861 cgctaagcga attcaaaata gaattagtga gcgcagaact agagagactg agaaaatcaa
     1921 agaacgacca agcgacacaa ccaacaacag aataggcgaa aaaaatagca cttatgcggc
     1981 gccacatata aaaatctaca cccaagtaac ttcaagatgc atgattaaag cccccccccc
     2041 tcaacaactt ccaccatgcc gtactttggg caccgattcc tgcaacttga agttaccatc
     2101 ggtatagtga aaaacataaa actcgatctc tacaattcac acagcgctac taatgaccca
     2161 catttaccat aacgtctcat tacgcgcacc gctaaaacgc gctgaacgcg attctgccgc
     2221 aaacaccgca aaaagagatg ttttccccgg ggtaaatcgg aaaaatgcgg caggatcgct
     2281 ttcagctcac tgcatagcta tgcatgaaag tgaatggcga tcggtttggt gacttacggc
     2341 gttcataccg tctgttttcg acagtttccc tccgggaagc taatctgcca taagcctgga
     2401 taacagggcc cggtgatact ccgtaatagc tatcagcacc tccgcatact ctctgtcccc
     2461 gacacgcttg ccgtcgataa agagtttttt ctgcaacgcc cccagtcgca tgacttcttt
     2521 cagcacccgg tcatcagtca gtgaattaac tttcttaccg agagctgccg cccgtaaaaa
     2581 accagaaacg gtcttgccac attcagcagc cctttttctg atttcctggt cttcttctgc
     2641 cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac ttcgttttgt
     2701 cataaaacat ggctccgtat ctgacatagg gtgtcggggc gaagccctga ccagggtaat
     2761 tgtaatagcg tgcatgtatg cgcggtataa caattgcaca tcctgtcctg tctgcaagtt
     2821 cgaattacca acaaagcact gctgtttttt ggcagtgaca gtgttgtctg actgaaatat
     2881 ccgtaggcag aatgccggcg gtaagacggt cgaaaagtgg tgcgcgatat aatcacacga
     2941 cgcaaatgac agacagaacg gagaaaacag aaag
//
