LOCUS       NZ_CP045774             5435 bp    DNA     circular CON 31-OCT-2023
DEFINITION  Bacillus paranthracis strain CFSAN068816 plasmid p3CFSAN068816,
            complete sequence.
ACCESSION   NZ_CP045774
VERSION     NZ_CP045774.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10574728
            Assembly: GCF_009648955.1
KEYWORDS    RefSeq.
SOURCE      Bacillus paranthracis
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Mafiz,A., Kastanis,G. and Gonzalez Escalona,N.
  TITLE     Closed genomes of twenty-eight foodborne pathogens from the CFSAN
            verification set by a combination of long and short-reads
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Mafiz,A., Kastanis,G. and Gonzalez Escalona,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) ORS/DM/MMSB, Food and Drug
            Administration-College Park, 5001 Campus Drive, College Park, MD
            20740-3835, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes, CANU v. 3.12.0, 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84, 43, 113, 105, 195, 264x
            Sequencing Technology :: Oxford Nanopore GridION, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/31/2023 05:35:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,867
            CDSs (total)                      :: 5,715
            Genes (coding)                    :: 5,522
            CDSs (with protein)               :: 5,522
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 15 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 64 of 193
            Pseudo Genes (incomplete)         :: 131 of 193
            Pseudo Genes (internal stop)      :: 39 of 193
            Pseudo Genes (multiple problems)  :: 35 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045774.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /strain="CFSAN068816"
                     /isolation_source="Mac and Cheese"
                     /db_xref="taxon:2026186"
                     /plasmid="p3CFSAN068816"
                     /geo_loc_name="USA:CT"
                     /collection_date="2009-06-11"
                     /collected_by="FDA"
     gene            complement(join(4897..5435,1..745))
                     /locus_tag="GH772_RS00030"
                     /old_locus_tag="GH772_00030"
     CDS             complement(join(4897..5435,1..745))
                     /locus_tag="GH772_RS00030"
                     /old_locus_tag="GH772_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016116854.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_190240640.1"
                     /translation="MSMSVSLKKATNKTNIKHNNRTMSEKEKERNSHIDYSKSDDNKYL
                     VQKDLKELYQEEFGEVLENYNAKQKRNYRKIDDYYKHIQFGKKTALQQEMIIQVGDLNN
                     FISNADYEKANEILLEWFKDFEKRNPNLKVYNAVIHNDEASPHMHLNFVPVASGYKRGL
                     EKQVSFDRAITQQDPTLDKTRPFDDWREKEVKILEKMLKKRGIERKLVGSNNYKDVNEY
                     KEKKDLEKEILSLENQLFEKKNELLEMNERLPGEIKIKAEREKKIEVINKGFLKKEKVE
                     KPTGNWIVGTKELKRVQGMINAGYSVKKDYDRLQRTDLVRENKELQGKVDSLADGLVEA
                     INKNTDLEDENRKLRKEISSLKAHIGDLKENIKVLYHNTKKVLGEHFKAFRGLVKNELD
                     MNGVDNQFEREHKKELKHKQRRHDMERF"
                     /name="plasmid recombination protein"
     gene            1564..2130
                     /locus_tag="GH772_RS00035"
                     /old_locus_tag="GH772_00035"
     CDS             1564..2130
                     /locus_tag="GH772_RS00035"
                     /old_locus_tag="GH772_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_000559145.1"
                     /translation="MHYNPYIYPYQYLYYVNVPIPMYNYGRQSVYCTFPNEIEHANRFD
                     SFHPSNGDRRISLTDYGPKPFVVNINEATKQNNTYRTALWTGTHLQVTLMSLNVGEDIG
                     LEMHSNVDQFLRIEQGQGIVQMGKSKDNLNFKRNVYDDFAIMIPAGTWHNLTNTGNIPL
                     KLYSIYAPPNHPFGTVHVTKADAIS"
                     /name="cupin domain-containing protein"
     gene            complement(<2225..2371)
                     /locus_tag="GH772_RS29085"
                     /pseudo=""
     CDS             complement(<2225..2371)
                     /locus_tag="GH772_RS29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335446.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /name="cell wall hydrolase"
     gene            2545..2985
                     /locus_tag="GH772_RS00045"
                     /old_locus_tag="GH772_00045"
     CDS             2545..2985
                     /locus_tag="GH772_RS00045"
                     /old_locus_tag="GH772_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_000168945.1"
                     /translation="MASTPYDPFRQLVNMRRDFDRIFSELPFDFGGDKNHFGNIKVDVY
                     ETENEVVATCDIPGLEKSEDVNIDIENNVLTISGSMNKTNETKEKNMYRKERYTGSFQR
                     VITLPSPVTIEGVSATYKNGVLEVKMPKTTPNNKKKIDVDFH"
                     /name="Hsp20/alpha crystallin family protein"
     gene            complement(4042..4662)
                     /locus_tag="GH772_RS00050"
                     /old_locus_tag="GH772_00050"
     CDS             complement(4042..4662)
                     /locus_tag="GH772_RS00050"
                     /old_locus_tag="GH772_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000162395.1"
                     /translation="MTKKIINFGQAEQKARKRDLEIEQKQFEEEHHGMDQDAVDEALKV
                     LSKATGGKEIYIGTKKSPQSKVRFAQSMQENLGYLYRQKYLTDREKIFLTEIIPYIAFS
                     SNCIVLDIKAKNPVPANITEIAKLIDASRQPTSTVINSLVKKGILFKGDSGVEGNNAKA
                     YAVFINPHVVYAGDKDNVNEALKVMFYKAMKMPILKDLPNKFF"
                     /name="hypothetical protein"
     Typing          complement(3..739)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="99.0"
                     /evalue="1.27e-159"
     Typing          complement(4904..5433)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.746"
                     /coverage="99.0"
                     /evalue="5.44e-89"
ORIGIN
        1 tttccagtaa ttcatttttc ttttcaaaaa gctggttttc caaagaaaga atttcctttt
       61 ctaaatcctt tttttctttg tattcattca catctttata attatttgaa ccaactaatt
      121 tccgttcaat accacgcttt tttaacattt tttctaatat cttcacttct ttttctcgcc
      181 aatcatcaaa aggacgagtt ttatctaatg ttggatcttg ttgtgtaatg gctcggtcaa
      241 atgatacttg tttttcaagt cctcgcttat acccactcgc cacaggcaca aaattcaagt
      301 gcatatgagg gctagcctcg tcattatgaa tgaccgcatt gtaaactttt aaatttggat
      361 ttcgtttctc aaaatcctta aaccactcta acaaaatttc atttgctttt tcataatcgg
      421 cattggaaat gaaattgttc aaatcaccaa cttgaatgat catttcttgc tgaagcgcag
      481 tttttttacc gaactgaatg tgtttataat agtcatcaat ttttcggtag ttacgttttt
      541 gtttagcgtt atagttctcc aatacttctc caaattcttc ttgatataat tcctttaaat
      601 ctttctgaac aagatattta ttatcatcag attttgaata atctatatgc gaatttcgct
      661 ctttttcctt ctcgctcatt gtcctgttat tatgtttaat attggttttg ttcgttgctt
      721 ttttgagaga aactgacatg ctcatgacga atcaactcct tgcctccact ataccaaaag
      781 ctttgttact ttggcaaagt aaccttttct gtattgtgac attccgctat gctccatgta
      841 cacaatgaaa aggctctgcg aggctgtttt ggctatcgcc aagccatgct ttgcatgaca
      901 aaaaacaaaa ccttggactc tgtccaaacc tgctggggaa ctcttcccca gaccccctta
      961 atccaactcc ccaacccctc aatccttgag gggctccctc gccggttggc agggtcaaaa
     1021 ggataccttt tgacaaccac aagagggttg catagtgagt gtgtcaactc cttatcctcc
     1081 cgccccctta cgggtttcgg tccggtaccg ttgacacacg ctatgcagct acctctacct
     1141 aattaaaaac ctaattttct actcaagtag cactacatag gttccccgct cttttgattt
     1201 ataaaatcaa cattctatga acttgttatt aagggatacc accaacattt tggaaaaaac
     1261 cacgctaaga gtatgcaaga ttttatgcaa aaaatcggaa gacataggga actatgggga
     1321 ggccgaaaag cccgccataa taggaatgta taagaagatg catagaatca tagaaaaaga
     1381 caaaggcgga ttccttgtga gagtagggat ccgcctttgt cttcactacc gataatgaga
     1441 cgttatgtat actagatttg tataacgtat tcatgtgttt ttaataagtc accttgaatc
     1501 taagtacatt tctttaacaa gtgcatagaa taattttgta aaaacgtgaa aggattgggg
     1561 aaaatgcact ataatcctta tatatatcca tatcaatatc tttattatgt taatgtacca
     1621 ataccaatgt ataactatgg aagacagtct gtttattgta cttttcctaa tgagatagag
     1681 catgcaaaca gatttgattc ttttcaccct tccaacggtg atagaagaat ttcgttaaca
     1741 gattatggac caaaaccatt tgttgttaat atcaatgaag caactaaaca aaacaatacc
     1801 tatcgtactg ctttatggac aggaacacat ttacaagtta ctttaatgag tctcaatgtt
     1861 ggcgaagata ttggtttaga aatgcattca aacgttgatc aattcttacg tattgaacaa
     1921 ggccaaggga ttgttcaaat gggcaaaagt aaagataatt taaactttaa aagaaatgtc
     1981 tatgatgatt ttgcaataat gataccagct ggaacatggc ataatctaac caatacaggt
     2041 aatattcctc taaaacttta ctcgatatat gctcctccta atcatccatt tggtactgta
     2101 catgtaacaa aagctgatgc cataagctga attaaactat taggggtagc atcaggatta
     2161 tgcggattta caacgttaag acaaaaaacc ttgtatcaat agagtacact ctattgatat
     2221 ataattgtac ttgaaaaatc acgtctctta ctgaccttaa attcaaaaaa tctaaacaat
     2281 ctgcttttaa tcggtttacg atgacattac caaccataag catccccagc tgtccttctc
     2341 cttcagcttc tgctctcatc atccttgcca tcagtgcaac atcactttcg gtatatttca
     2401 ctcgttacac ctttatcact ccttttattg aattatatga acagtgctca acaaaaatac
     2461 gttgttgtcg attcctaatg aacctgttta aatatgaaaa aatcagggaa acatatgaat
     2521 gataaatgaa ggaggaatgc aattatggca tcaacaccat atgacccatt tagacaattg
     2581 gtaaatatgc gaagagattt tgaccgtatc ttttctgaat taccgtttga ttttggaggg
     2641 gataaaaatc actttggaaa cattaaggta gatgtttatg aaactgaaaa tgaagttgtg
     2701 gcaacatgtg atatacctgg acttgaaaag agcgaagacg tgaacattga tattgaaaat
     2761 aatgtattaa ccattagtgg ttctatgaat aaaacaaatg aaacaaaaga aaagaatatg
     2821 tatagaaaag aacgttacac aggcagtttc caacgagtta taacacttcc tagtcctgta
     2881 actattgaag gtgtatctgc gacatacaaa aacggtgtgt tagaagtaaa aatgccaaaa
     2941 acaacaccaa ataataaaaa gaaaattgat gtggattttc attaaaaatg ggcgatttat
     3001 atcaagtgat gaaagaattc gaaagggact atttagagga aatatctaat atacagccag
     3061 aattagtgga cctgcttata ctaacttgtc attcttcaga aattaaaaaa aaagctatat
     3121 tagatgaact tactcataac attaaattat catatatgaa aaataatatg aataatcttt
     3181 cgattagcaa tcgttgaata aggactgtcg aatacgacag tcctgttatt aagggtagca
     3241 tcaggattat gcggatttac aacgttaaga caaaaaacct tgtatcaata gggtctactc
     3301 tattgatatg gaatcaataa tacgtaaaat aagagttttg aatgtatcat atgacttgtc
     3361 tcaaaaccct tatcgttgtt aagttagcct ttttttcttc tcagaattgc ttaacgttgt
     3421 aaatccgcat tttcgggact gtacccctat tagaaatagt aaaaagaatc ttcaatttaa
     3481 gatccttttt ttatgccgat aacgaagatt atgtaaatga gctgtctata tggacggctt
     3541 attatttttt cgcttaagat tttccccttg tttttgggtg gaatggtgaa ggctggggtg
     3601 aagcgtagcg aggggctagc ccctaaatat gcatcaaata catattgcaa aagaataatg
     3661 tatcggttgg gagaactggg tggttcggct ggtacaattg cttgttcaag tcgagcaact
     3721 taaattctcg tttttggtct gttcggcttg gcgaaagtca aaaaccagcc gaaaaaagtt
     3781 ttgcaaggag ggagcaaagc ggacaaggtg gagatttttt gaatggggtt ctcaaaaaat
     3841 actacctgtt cctttcggaa cttttaaccc tatatataga gtgagaagag agagactttt
     3901 aaaagtgtaa aaaatgttga tatatcagtg tttttaaaga ttttcgtgtg tcacaaatat
     3961 gtgacacgtg tgtcacatat ttgtgacaca cgaaataata aaaaatcgta gttatcgatt
     4021 tgataactac gatttttatt ttcaaaaaaa cttattaggt aaatccttta aaataggcat
     4081 cttcatagct ttataaaaca taacttttaa tgcttcattt acattgtcct tatctcctgc
     4141 ataaaccaca tgaggattaa taaatactgc ataagcttta gcattatttc cttcaacgcc
     4201 tgaatcacct ttaaataaaa ttcctttttt aactaaagaa ttgattactg tacttgtagg
     4261 ttgtctagaa gcatctatta attttgcaat ctctgtaata tttgcgggta cagggttttt
     4321 cgctttaata tcaagaacaa tacaattact agaaaaagca atataaggaa ttatttcagt
     4381 taaaaaaatc ttctccctat ctgttaaata tttttgccta tacaaatagc ctagattctc
     4441 ttgcattgat tgagcaaacc tcactttcga ttgaggactc tttttagttc ctatgtaaat
     4501 ctctttacca ccagtagctt tacttaacac ctttaaagct tcatctactg cgtcttgatc
     4561 catcccatga tgttcttctt caaattgttt ttgctcaatc tctaaatctc tttttctcgc
     4621 tttttgttca gcttgtccaa agttaataat cttttttgtc atgtgttgtt gctcctctca
     4681 ttttgtgagc accaacataa ataccaaaaa tcataattat ggtattaccc taataataaa
     4741 attatgataa tctataaaca gatattgatt tttaggtatt ttcgttgaat accctttgaa
     4801 acgagccatg ctccaacatg gctcgttttt attttatcac acatataatc ccaagaaatg
     4861 gtcaaacaaa atttagagaa acttttactc actttgttaa aaacgctcca tgtcatgtct
     4921 ccgttgttta tgcttcagtt ccttcttgtg ttcacgttca aattgattgt ctacaccatt
     4981 catatccagt tcattcttaa caagtcctct aaacgcctta aaatgctctc caaggacttt
     5041 tttcgtgttg tggtataaaa cctttatgtt ttcctttaaa tctcctatat gggcttttaa
     5101 cgagcttatt tccttacgta gttttctatt ctcatcttcc aaatctgtat ttttgttaat
     5161 agcttcaacc aaaccatctg ctaaactatc aactttacct tgtaattctt tattttctcg
     5221 aactaaatct gtacgctgca aacgatcata atcttttttc actgaatatc cagcattaat
     5281 cattccttgc actcttttta attctttagt tccaacaatc caattccctg ttggtttttc
     5341 tactttttct ttttttaaaa aacctttatt aataacctct attttctttt ctctctcagc
     5401 tttaatctta atttctcctg gcaatcgttc gttca
//
