LOCUS       NZ_CP045772             3091 bp    DNA     circular CON 31-OCT-2023
DEFINITION  Bacillus paranthracis strain CFSAN068816 plasmid p5CFSAN068816,
            complete sequence.
ACCESSION   NZ_CP045772
VERSION     NZ_CP045772.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10574728
            Assembly: GCF_009648955.1
KEYWORDS    RefSeq.
SOURCE      Bacillus paranthracis
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Mafiz,A., Kastanis,G. and Gonzalez Escalona,N.
  TITLE     Closed genomes of twenty-eight foodborne pathogens from the CFSAN
            verification set by a combination of long and short-reads
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Mafiz,A., Kastanis,G. and Gonzalez Escalona,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2019) ORS/DM/MMSB, Food and Drug
            Administration-College Park, 5001 Campus Drive, College Park, MD
            20740-3835, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes, CANU v. 3.12.0, 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84, 43, 113, 105, 195, 264x
            Sequencing Technology :: Oxford Nanopore GridION, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/31/2023 05:35:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,867
            CDSs (total)                      :: 5,715
            Genes (coding)                    :: 5,522
            CDSs (with protein)               :: 5,522
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 15 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 64 of 193
            Pseudo Genes (incomplete)         :: 131 of 193
            Pseudo Genes (internal stop)      :: 39 of 193
            Pseudo Genes (multiple problems)  :: 35 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045772.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /strain="CFSAN068816"
                     /isolation_source="Mac and Cheese"
                     /db_xref="taxon:2026186"
                     /plasmid="p5CFSAN068816"
                     /geo_loc_name="USA:CT"
                     /collection_date="2009-06-11"
                     /collected_by="FDA"
     gene            1084..2328
                     /gene="mobV"
                     /locus_tag="GH772_RS00005"
                     /old_locus_tag="GH772_00005"
     CDS             1084..2328
                     /gene="mobV"
                     /locus_tag="GH772_RS00005"
                     /old_locus_tag="GH772_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951564.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951564.1"
                     /translation="MAHVEKYTKGSVQGLSIHWDRKTENHSNQEIDNERSNLNYDLCEK
                     EGDTLSRMNDRLREVHCLNRKDVKVCSDWVVTLPENLKGTSEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHKDETTPHMHFAFMPVVWDEKKQREKVSAKEVLTRKDLKTFHQDLDT
                     FLKKEIPHIYKDGILNDKTIGVDTVKDLKKHSLEIEKQKKAMDAEIKIKEKDLERKIQS
                     LDKEFESKKKKLLDLSEQLPQEIKINAKGKEKKTEVVKKGLFKTETITKNTGNWIIGTN
                     ELKRVQKMVNAAYMVKRDYERLQSTDLVEENKKLHLQVEGLSNNLKASHQINAELRERN
                     KELHTKIGSLQAHINDLKINVKVLYQQTKKVFKEQFKTFRGLVKNELVGREVEDYFERE
                     HKNEMTKQRGYDMER"
                     /name="mobV"
     gene            complement(2358..2816)
                     /locus_tag="GH772_RS00010"
                     /old_locus_tag="GH772_00010"
     CDS             complement(2358..2816)
                     /locus_tag="GH772_RS00010"
                     /old_locus_tag="GH772_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001125937.1"
                     /translation="MPKQKTENEIIYNGKSRWRNLDTGEIIETDEVIKKTPRNGFMITY
                     LTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVSRQTVIDTLKTLE
                     QAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFNNNKAPINAVE"
                     /name="replication/maintenance protein RepL"
     Typing          complement(2359..2816)
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          944..2325
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.783"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattttttga gaaaaccact caaaaaatct ccactctttt gctattgcat agtgaaattc
       61 ggcaggtgtg agcatgtcgc caagccgaac ataccaaaaa cgaggattta agttgcacga
      121 cttggactag caattgtacc tgccgaaccg ccttgatcta tcgttcaaga atttcactaa
      181 tgccatataa aattacgtta agttcctggg ggctagcccc caaaccccca gccagcactc
      241 acacccaaaa acaaggggga taccaataaa aaagtcaaaa tccactatat aggattttga
      301 cttttttatt ggtattgaac tacaagggtc cctacaggct gtttttgaac acccgtagtt
      361 ctttttaaaa gggattaatt tatcagattt attaaatgaa taattcataa aatccccttt
      421 caatcactct gttgtatggc aatttatcaa gtaggcagtt gtacataagt agtgtatgca
      481 ggggacaaaa atgtgtatat aactggataa gcctttttta tttttggctt ggtatgaaaa
      541 aagaacccta atgttggata gggttctttt taggggtaac agttgaaggt tgaaggtata
      601 tttcatattt attatgtact ttactcttat attcattcga ataggaaaca catatgtttg
      661 gagtaatttg caaagcgatt gttggaaaag gaaaaattag cggtaagtgt caatggtaag
      721 cggactggaa ggggtcagcg gtttgtgctg atccatgaaa ggagcattaa ggaattgaca
      781 cgtgcaattg attcttcacc ctcttggcct gtcaaaacgt caaacgtttt ggcctgccaa
      841 ccggcgaggg agtccctcaa ggattgaggg gttggggagt tcgattgatg gggtcaaggg
      901 gaagagttcc ccttgtaggt tcggacaaag tccgaggttt ttttgccatg caaggcatgg
      961 tttggcgaag ccaacgagcc tcgcagagca cgtccaaatg aaatttggta taacgtgcta
     1021 gaccaaatta ttggattctc tcaatcttcg tgctatgctg attaccaaaa ggggggctta
     1081 cttgtggctc atgtagaaaa atatacaaaa ggcagcgtac aaggattatc gattcattgg
     1141 gacagaaaaa cagaaaatca ttcgaatcaa gagattgata atgagcggtc aaatttaaat
     1201 tatgatttat gcgaaaaaga aggagataca ctttcccgca tgaatgatcg attacgtgaa
     1261 gtacattgtt tgaatcggaa agatgtaaaa gtttgtagtg attgggttgt tacgttacca
     1321 gaaaacttga aggggacttc agaaaaagaa caacgtgaat tttttgaaaa aacctatgag
     1381 tttttagcga atcgttatgg cggtgaaaaa aatgtattgt ccgctaacgt acataaggac
     1441 gaaacaacac ctcatatgca ttttgctttt atgcctgttg tttgggacga gaaaaaacaa
     1501 cgggaaaaag tttcagccaa agaggtttta acaagaaaag atttaaagac attccaccaa
     1561 gatttagata catttttgaa aaaggaaata ccacacatct acaaagatgg aattttaaat
     1621 gataaaacga ttggtgttga taccgttaag gatttaaaaa agcattctct agaaatagag
     1681 aaacaaaaaa aggcgatgga tgcggaaata aagataaaag aaaaagactt ggagagaaaa
     1741 atccaatctt tagataaaga atttgaaagt aaaaagaaaa agcttttaga tctcagtgaa
     1801 caattaccac aagaaatcaa aatcaatgca aaagggaaag agaaaaaaac agaggttgtg
     1861 aaaaaaggat tatttaaaac agaaacgata accaaaaaca ctgggaattg gattattgga
     1921 acgaacgaat tgaaaagagt acaaaaaatg gtgaacgctg cctatatggt caaaagggat
     1981 tatgaacgtt tgcaaagtac agatttggtg gaagaaaata aaaaattaca cctgcaagta
     2041 gagggcttgt ctaacaattt aaaagcatct catcagatta atgcggagtt gagagagaga
     2101 aataaagaat tacatacaaa aataggctcg ttacaagctc atataaacga tttgaagata
     2161 aacgtaaagg ttttatatca acaaacgaaa aaagtcttta aagagcaatt taaaacgttt
     2221 agagggcttg ttaagaatga actagttgga agggaagtag aggattactt tgagcgtgaa
     2281 cataaaaacg aaatgactaa acaacgagga tatgatatgg aaaggtaaaa ccgccttaga
     2341 atcaattctt gggcggttta ttcaactgca ttaattggag ccttattatt attaaaatct
     2401 tcgaatctag caagtaagta tttctctttt gtatctttac cacgatgaac aaggcttgga
     2461 tgtatcatta ttgctccagt tcttctagta attatttggg cttgctccag tgtttttaat
     2521 gtgtctatta cagtttgtct agataccttg cttttttcgg tcaattctct tgtagttata
     2581 attaacgtat ttgttgattt gtccatgttt tctaatatgt attttacaac ttgcattttt
     2641 ttattaccta aactatctat taattgaacg attgcagtta aatatgtgat cataaaacca
     2701 tttctaggag ttttctttat gacttcatca gtttctatta tttcacctgt atctaggttt
     2761 ctccatcgac ttttaccgtt atatattatt tcgttttcag ttttttgttt tggcatatta
     2821 tcacctttct cctatatttt tatatttcca ttttatcata attagaccaa aaggtcaaaa
     2881 aaaattagac caaaaggtat aaaaaattag accaaaaagt ataaaaaatt agaccaaaga
     2941 aaatgccctt caatagctat atatcagctt aaaatctact ttttaccccc tgcttactct
     3001 tttctatata tttaaggcga taaatatcgc cttaaatata ttcttttttc atttttttta
     3061 gagggctttc cacaatgcaa tagtcgagta g
//
