LOCUS       NZ_CP045685             2310 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_15, complete
            sequence.
ACCESSION   NZ_CP045685
VERSION     NZ_CP045685.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045685.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_15"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            complement(join(2114..2310,1..229))
                     /gene="mobA"
                     /locus_tag="F8D37_RS28520"
     CDS             complement(join(2114..2310,1..229))
                     /gene="mobA"
                     /locus_tag="F8D37_RS28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001107325.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKNKYPGSDLFK"
                     /name="mobA"
     gene            1583..2080
                     /locus_tag="F8D37_RS28525"
     CDS             1583..2080
                     /locus_tag="F8D37_RS28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001027759.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNITQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     Typing          complement(976..1234)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="100.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 ataatttctt ctgcatccga ccaagtcgga ttaattcatc taaatacctg gaaggtacgc
       61 gctgttttat ttcattattt aaacttgccg tcctgatata ttctgaaatt gtcagtcctt
      121 cgttcttcgc tcgccgtctg atttcgcttg cgactctgtt atttactcta agggtgataa
      181 ccttagttct ctgccgtact tctgaaccac tacgtttatt tttagccatc gcaatgactc
      241 tccattttgc acgtttgggg tgtcggggct tgccctgaca ggaggtattc tcatgcaagc
      301 gaatgtattc gcggtatgac gaatacacat cctgtccaat tttccgctac aacttttcga
      361 cgaaaataaa ataccgcgtc gtaacctggt atttctgata caaaaaaatg atgcttcatt
      421 caatattatt tacacagatt ttaaaaatta ttgcttgtta tttaataaat aacatcgaat
      481 taattgatgt ctttgttgtc tgtcgtttgt ggattattgg agggataaag attagatctt
      541 cctcgttata taatccattg cctgttaatg atacggagtg tgtccaagga acaggaagcg
      601 gaagagcaag atagtagcat gtacataaaa tataaatccg tgagttattg atatcgcgtt
      661 tatatattaa aaatataact taacattgct cttaatattt attttgtcct ggcaatggga
      721 gcattaatcc ccgtgcagtg ctccgccagc tgctgacaac ctcgacatat aaccatcgat
      781 acgatagaac atcagggcgc gtttccggca agaactaacc atggggatgg tttgcgtgcc
      841 gacaaacgcc ggaacgcgcc tcaaatccac caagccacca cggttccggc agcgccttga
      901 gcgcaaccgg aaaccgtctg gggagaaccc cagaccccca ccgctgcaac atcatcggtc
      961 tacgaccacc taccaaccga gtggctacct gtgccgtgcg aacaggcttt gcagggggct
     1021 ggcgcggttc ctggcgacct gaaagcccct ggttaggggt atcggcgcgc ctggcctcct
     1081 gaaaccctct gtaagcgttt ttcgcgcctt tcatgccctg acgggcattt aaaccccgtt
     1141 tcgtgcgtct gggaggctct gagcgcgttt aacagatgac cagcgactcg atcgcctgag
     1201 atcaaatcgg gatcatcaac gatatgcgag aggtggcacc gctcgccgca gccccagcga
     1261 ccgaacgtag tgagcgagtg ggcgaggaag cggaagagaa ccggaagcta cattgagcac
     1321 gcacgctcaa tattaaaatt gtcacttcgt aatatagaga ttatgcctcg tgcttatgga
     1381 tacatttaaa aatataagat atccgaatta ggctacatac gggctgatcg ccaatgttgt
     1441 aacgtgtaga agatacacct gtaacgtgta caggatactc ctgtaacgtg tgggcggtat
     1501 tgtcgtaatc tatattgagt gacattttgt ttttttggca ttatattgtc tgtcaccttt
     1561 ataaagtgac aaaggcgata aaatgaacat tactgaaaga acggatagca agaaaaaagt
     1621 tttaagacaa aatattactg aggttataga tagagaaact ggaaacataa cacaagaaat
     1681 aaccgatata acagttcaat ttccacaaga acctgcgtat gtgaaaattt atattgataa
     1741 tttatgtgct gtcacaaaag cacctgattc tctaaaagat gttttatttt taattcttag
     1801 gaaattggat tatgacggat atattgcatt atcaactaga taccgtaagg agatctgtaa
     1861 attattggga ataaaagacg gaacattacg taacagattg tattcattat caaaaatggg
     1921 gattattgct agttgtggcg gtaatgaata tcaggcgaac cctaatttat ttgctcgtgg
     1981 tgaatggaaa aaaataattg aacagcgtcg cgagtttgaa atgagaatta aatattcacc
     2041 taacggtgaa actattataa caaccgaaga aataaaataa tgtgcaccta aataaggtct
     2101 ttttaaaaag accttatttg aataaatccg aacctggata tttattttta tgtatccttt
     2161 ttattatttt tatttcatcc tttattaaat ccatttcatt atatacatca gaaattcgct
     2221 tcgttactat tttcatttca tcgcaaagta aaattatttt atgcatcacc tcgaggtact
     2281 ccttatcctt cggccgcttt cccttatcaa
//
