LOCUS       NZ_CP045684             3373 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_14, complete
            sequence.
ACCESSION   NZ_CP045684
VERSION     NZ_CP045684.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045684.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_14"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            complement(join(3203..3373,1..6))
                     /locus_tag="F8D37_RS28495"
     CDS             complement(join(3203..3373,1..6))
                     /locus_tag="F8D37_RS28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_049883857.1"
                     /translation="MRRLNEWESAFAELQKMHEVTQRNNAILNERVVILSQQVQRLAGQ
                     VDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(70..630)
                     /locus_tag="F8D37_RS28500"
     CDS             complement(70..630)
                     /locus_tag="F8D37_RS28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<659..1318)
                     /locus_tag="F8D37_RS29425"
                     /pseudo=""
     CDS             complement(<659..1318)
                     /locus_tag="F8D37_RS29425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1308..1631)
                     /locus_tag="F8D37_RS28510"
     CDS             complement(1308..1631)
                     /locus_tag="F8D37_RS28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1695..1886
                     /locus_tag="F8D37_RS28515"
     CDS             1695..1886
                     /locus_tag="F8D37_RS28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1968..2048)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(3..1318)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.583"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccgcatatag tcctgttgca aatgctctaa ggcgttcagc aaatgccctt ccagttccgt
       61 catactcgtt tactccctga ccagcctcac ggcgttcttg tcgcccaccg tccaggtgtt
      121 ttcccctttc accccgtccg gcaacaccag aaatttcccg ttctcgccat tgacgaacgt
      181 caccccccag gtttttgcct cgagtttcgc cagcgtgttt tcctgctctc tgatagtcag
      241 gatatttttc gcaatcttgc tttcctgcca ctccatcagc ccccagcacc ccaggaaaat
      301 cccgcccgtg acgctgagcg cggtcagcgc cggaaacagt atccgccctt tgatcacccg
      361 ccagagcagt tttttctgct gccgggacag ttcggcctct gtggtgctga actgcatgct
      421 cacggtggtg ctcagcgtat ccagttgctt tttcacggat gttgtatgtt cgctgatagc
      481 gtctctgatt ttctgcctgt ttaagctcaa tgccttgtct acagactctt cgagcttcct
      541 gaactcgctg ttcagcatgt tctcggtaga agcggctcgc tctttcagtt tcttctcgaa
      601 gtctgttccc atttgtaaaa gattgctcat acagcgcccc ctttagcctg agattccgcc
      661 ccccctccgg gtcggcaata ctgatgctgc ttttggttgt cctcaccacc tcaaaacctg
      721 ccgctgtaag cgcctctgtg acatcctgac gcgtttttat tgctccggca tggtaaagag
      781 cctcaagacc ccgtgtaatc gcttctgcgg cctcctgttt cgcttttggc agattatttg
      841 gcgtgacaag cgtccgtctg ttctccggtg cgttcgggtc acgcagcccg taatgatgat
      901 taaccagcgt ctgccaggca tttatcctcg gcctgtccgc acggtcgtag tacggctgga
      961 ggcgcttacc gctctgtagc tccatgttcg ggatgacaaa attcagttcg agccgcccct
     1021 tatcctggtg ttccacccag agaatgctgt actggtcttt ttcgagaccg ggcataagaa
     1081 cacgctcaaa gctcgtcatc actctttcgc gttcacccgg tggtagctcc ttctccgcga
     1141 acgacagaac gcctgacgtg tatttcttcg aaaatggcgt ggaatcaatg agttcccgca
     1201 cttcttccgg gttaccccgg agcaccgttg cgccttcgcg gttacgctcc cgccccagca
     1261 ggtaatcgac cgggccactt ccaccgcctt tccccctggc atgaaattta actatcatcc
     1321 cgtaccccct gttccctgac agcctgccgc aactggcgca attcaccctc gatggccatc
     1381 agtgcggcca ccacatgaac ccggtcaatc gaagaccact ggccgctgtt caccttccgg
     1441 gctgtctggt tcaggttgtt cccgatggca gccagatgcc gcagcaatgg cggtgccagt
     1501 gtcggcagtt ttcctgtgcg tgcaaccggc tcccccagac agacccgccg catccattcc
     1561 gccagtcgtt taccctcgca gcgttccagt aaccgggcgt gctcctcatc ggtaacccgt
     1621 atcgtgagca tcttctcgcg tttcatcggt atcattcccc ctaacaaatc ccccatacaa
     1681 ggagccttaa gaccatgacc aaacaggaaa aaacagccct taacatggct cgctttatca
     1741 gaaaccagac gctaaccctg ctggaaaaac tcaacgatct ggacgcggac gaacaggcag
     1801 acatctgcga atcacttcac gaccacgccg acgaactcta ccgcagttgc ctcgcacgct
     1861 tcggcgatga cggtgaaagc aactgaaaca tgcagctccc ggagacgttc acagcttgcc
     1921 tgtgagcgga tgccgggagc agacaagccc gccagggcgc gtcagtgcgg gtgtcggggc
     1981 gcagccctga cccagttcac agagcgatag cgatgtgtat actggctttc ctcattttgc
     2041 atcggtgcag agtggatggg aagtgcacca tgtcaggagt aaaaagccgc acagatgcgt
     2101 aaaaacagcg cgcccgataa acaacacaca cgcggacact ggcacaggcg cgtaagtgca
     2161 caatgcttgc ctctgtgact cttccgcttc ctcgcccact cgctcactac gttcggtcgc
     2221 tggggctgcg gcgagcggtg ggatcactct cgttgtgatg cagatccgtt tttttttttg
     2281 cactggagtt atcaggagta gcggtgcaca tgggatcgtg cgatatgctg gatggtggaa
     2341 ggtcggaggg aacggcaaca actcacccct cccagtgaag ggacgggtag tttgccgctc
     2401 tcgatatgcc ggacggagcc aaggccatcg atcacggggc gcgtcagtgg aagaacccgc
     2461 gcctgcgtga cgtatgcgtc ttcgccgcac catctcaccc gtcacgccac cacataccgg
     2521 gaactgccgc caaggtctaa ctcaaccaat ctagccgcgc tacatacggt ttttgtggca
     2581 actcatcagg tgtccgctgt cgatgaaact ctctgcgccg gtagcggaac tctcacgcgt
     2641 gcggtcgccg tcgtcacagc tcccccaacc gcaaacgatt ctcacaaata cgcaagtatc
     2701 tgcaaaaaca ggtttttttc ggggttcctg ggagactgtg ctgttgaaaa actcttgaag
     2761 aatacagaag gctcatgcaa actgaatgaa ctggtttctg ttgtggatct caacagtttc
     2821 cacaggaaca ttgaggaggt cagagacctc aatgaacccg aaacagctcg ccttcctggc
     2881 gagctgtttt cgttctacgc ccaccgacga aagatcgcaa gtgcaataac gagatttttc
     2941 acgtctttac gctcaaaaag taaaaaaacc atgttttccc ctggctgaat ccccaaaaac
     3001 gcgtgaaatg cgttttcagg atgttgcata gctatgcaca aatacggcac acgatttgtt
     3061 tcgctcgccc cagccgtcga acgggtcaaa atggcaagat ttcgtcactt ctccggtcac
     3121 atctccattg atggcccact ccgaacccgt gccgcgcgtt cctgtacctc atgtctgtac
     3181 cgggcacgct cctgttcatc ccttagctgt tgttcgcgat aaataaccga ctcaagcgat
     3241 ccacttgccc ggctaatctc tgcacctgct gactcaatat cacgacgcgc tcgttcagta
     3301 tcgcgttgtt ccgttgcgta acctcgtgca tcttctgcaa ttccgcgaag gcgctctccc
     3361 actcgttcag ccg
//
