LOCUS       NZ_CP045683             3770 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_11, complete
            sequence.
ACCESSION   NZ_CP045683
VERSION     NZ_CP045683.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045683.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_11"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            complement(join(3129..3770,1..27))
                     /locus_tag="F8D37_RS28475"
     CDS             complement(join(3129..3770,1..27))
                     /locus_tag="F8D37_RS28475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059034.1"
                     /translation="MSDSGLMMGVITGDLYRSSTDYERGVSYIGLMEVMLEKIKKNNRL
                     RVSDVDFFRGDSFQITSKDPINLLSIAVYIRAYLRSYSEKNDGKGMYDARLAISIDKVN
                     KYRRDINSIFESAQIKSGKSLDLIENDEMMVFDSSLYDLKEIYSPSIILLDALLSMLSK
                     PQSEILREIVYDRDASISEIAERLGKSKQVVYKTIKRGAVDKIIKFLDAHLSLLGMRFF
                     "
                     /name="hypothetical protein"
     gene            complement(415..738)
                     /gene="mobA"
                     /locus_tag="F8D37_RS28480"
     CDS             complement(415..738)
                     /gene="mobA"
                     /locus_tag="F8D37_RS28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052522663.1"
                     /translation="MTKRSGSNTRRRAICRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1022..1216
                     /locus_tag="F8D37_RS28485"
     CDS             1022..1216
                     /locus_tag="F8D37_RS28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1703..1852
                     /locus_tag="F8D37_RS29505"
     CDS             1703..1852
                     /locus_tag="F8D37_RS29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162847635.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPGRHDKAPLAAAIDKSRCARDFR
                     ECLK"
                     /name="replication initiation protein"
     gene            complement(2120..3118)
                     /locus_tag="F8D37_RS28490"
     CDS             complement(2120..3118)
                     /locus_tag="F8D37_RS28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="WP_001477600.1"
                     /translation="MGHGVTVLFLLLLLVHIIFDFYLQTEEMVREKAFDNGIGRALKAH
                     LFHAVMHGFFLCLSCFAFIYVLLGCTNLLNFMLFVLLFSLFTFFHFAIDVAKSITSLKV
                     KGFYCFAVFLFDQVLHILTIFFVIYYFFCDYGVYIYDFNFIKYVGGVLIFACSIAFLLK
                     PSSIIVYLFFKSSGIGDGSEKVKITKSKIAELLYIVLNKELGDSDVKDCKVAVNKYKKK
                     AEEIVIGISDGQADIDIRTISKVNNAGRFIGYLERLIIFLFLILGSVTAVVAVLAMKTA
                     LRFSDLKDDNDSGKAEYIMIGTFFSLIVTVIISVLARMYLIEIGIKINDFS"
                     /name="DUF3307 domain-containing protein"
     Typing          complement(748..984)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          1252..1772
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.687"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(419..738)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.44e-61"
ORIGIN
        1 ccccatcatt aaccctgaat cactcatttc atcccccaaa ctaaaataat tcttgacaga
       61 ttgcacaacc gaaaaggaaa atagtcaacc aaaaaggttg aaaaacaaaa aagtaaccca
      121 ttttggttta caccaataac ttgatacttc ttggctctaa ggcgctcaat attaaaattc
      181 agttgtcacc ctgcatttaa cataaagcca taattgtacg caccgctgaa acgcgctgaa
      241 cgcgattctg acgcagacac cgcaaaaaga gatgttttac cccgggaaaa caggaaggat
      301 gcgtcaggat cgttttcagc tcgctgcata gctatgcatg aaagcgaatg gtgatcactt
      361 tgggagctta cggtgttcat accgtctgtt ttcgacagtt ttctctccgg gaagctaatc
      421 tgccataagc ctggataaca gggcacggtg atactccgta atagcgatca gcacctccgc
      481 atactccctg tccccgacac gcttgccgtc gataaagagt tttttctgta acgcccccag
      541 tctcataact tctttcagta cccgatcatc agtcagtgag ttaactttct taccgagagc
      601 tgccgcccgt aaaaaaccgg aaacggtctt gccgcattca gcagcccttt ttctgatttc
      661 ctggtcttct tctgccgtta gacgaacagg gcgacagata gcccgcctgc gcgtattact
      721 tccacttcgt tttgtcataa aacatggctc cgtatctgac atgggtgtcg gggcaaagcc
      781 ctgaccaggg taattgtaat agcgtgcatg catgcgcggt ataacaatta cacatcctgt
      841 cctgtcagca agttcgaatt accgacaaag acacggcagt tttttggcag tgacagtatt
      901 gtcaggcaga aatacccgtc aaagcgtcag acggcagaat accggcggta aaacagtcga
      961 aaagtggtac gcgatataat cacacaacgc agatgacaga cagaacggag aaaacgaaaa
     1021 gatgaacaag cagcagcaag ccgtactcaa catggcagga ttcataaaaa gccagagcct
     1081 gacgctgctc gaaaaactgg acgcactcga tgctgacgaa caggccgcca tgtgtgagaa
     1141 gctgcacgaa ctcgcggaag aacttcagaa cagcatacag acacgctttg aagcggaaaa
     1201 tcgcaccggg atataacgca tccggacagg atgcagaacg gcatagagga gcgtaaggag
     1261 aaaatgtcac ataaggcgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt
     1321 tcgactgcgg cgagcggtgt cagctcactc aaaagcggta atacggttat ccacagaatc
     1381 aggggataaa gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag aagccagcgc
     1441 cgcagacgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggaagctc
     1561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1621 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt
     1681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1741 atccggtaac tatcgacttg agtccaatcc gggaagacac gacaaagcgc cactggcagc
     1801 agccattgat aagagtcgat gtgcgagaga ttttagagag tgcttgaagt gatggccgga
     1861 ctacagctac actaaaagaa cagttttggt ggctcgtctc gcacaagaca gttacctggt
     1921 taagcagttc cccaactgac ttaaccttcg aaaaaccacc tccccaggtg gttttttcgt
     1981 ttccagtgca agagattacg cgcagatata aatgatctca agaagatcct tctcagatac
     2041 gaaaatgcat catcattcca ttgatatcat ttctcatcat tgcaaccatc atcttcttat
     2101 gtgagaaact gagctcaaac taactaaaat cattaatttt tataccaatc tcaatcaagt
     2161 acattctggc taacacagat attatcacgg ttacaataag actaaaaaat gtaccaatca
     2221 ttatatattc agccttccca ctatcattat catcttttag atcactaaat ctcagagctg
     2281 ttttcattgc taacaccgcg acaaccgcag tgaccgagcc taaaatcaaa aacaaaaaaa
     2341 ttatcaatct ttcaaggtaa cctataaatc taccagcatt attaactttg gaaatagttc
     2401 tgatatcaat atcagcttgc ccatcagaaa taccgattac aatttcctct gcttttttct
     2461 tgtatttatt tactgccacc ttgcaatctt taacatcaga atcacccagt tctttattca
     2521 atacaatata aagcaattcc gcaattttac ttttggttat tttaaccttc tctgaaccat
     2581 caccaatacc tgatgattta aagaataagt aaactataat agatgaaggc tttagtaaaa
     2641 acgctataga acaagcaaat atcaacaccc cgccaacata ttttatgaaa ttaaaatcat
     2701 atatatatac gccataatca caaaagaaat aataaataac aaaaaatata gttaaaatat
     2761 gcaaaacttg atcaaacaag aaaacagcaa aacaataaaa accctttact ttcagagagg
     2821 taatcgactt tgcgacatct attgcaaaat gaaaaaatgt aaataaagaa aacagcaaaa
     2881 caaatagcat aaagtttaat agattggtac agccaagcag tacatatatg aaagcgaagc
     2941 aggatagaca tagaaaaaaa ccatgcatca cggcatgaaa taagtgagcc tttaaagccc
     3001 taccaatgcc attatcgaat gccttttctc taaccatctc ctcagtttgc aaataaaagt
     3061 caaaaattat atgaaccaaa agcaacaaca aaaatagaac agttacaccg tgccccatac
     3121 cccctccatt aaaaaaatct cattccaagc agtgaaagat gagcatccaa aaactttatt
     3181 atcttatcaa ccgctcctct ttttatggtt ttataaacaa cttgtttact ctttcccagt
     3241 ctttctgcta tttcagaaat acttgcatcc ctatcatata caatctccct taggatttct
     3301 gattgaggtt tggacaacat tgaaagcaat gcatcaagaa gaataataga tggggaatat
     3361 atttccttta aatcataaag tgatgagtcg aagaccatca tctcatcatt ttcaatgaga
     3421 tctaaacttt tccctgactt tatctgagca gattcaaata ttgagtttat atcccttcta
     3481 tacttattaa ctttatctat ggatatcgcc aagcgagcat catacatccc tttaccatcg
     3541 tttttctcac tatatgatct taaataagcc ctaatataaa cggcaatact taataaatta
     3601 ataggatctt tgctggttat ctgaaatgaa tcccctcgaa aaaaatcaac atcactaact
     3661 cttaacctat tgtttttctt tatcttttct aacataacct ccatcaagcc aatgtaagaa
     3721 acccctcgtt cgtaatcggt actcgagcgg tacaaatcac cagtgataac
//
