LOCUS       NZ_CP045682             4510 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_10, complete
            sequence.
ACCESSION   NZ_CP045682
VERSION     NZ_CP045682.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045682.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_10"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            complement(565..921)
                     /locus_tag="F8D37_RS28445"
     CDS             complement(565..921)
                     /locus_tag="F8D37_RS28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1125..1445)
                     /locus_tag="F8D37_RS28450"
     CDS             complement(1125..1445)
                     /locus_tag="F8D37_RS28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1628..1870
                     /locus_tag="F8D37_RS28455"
     CDS             1628..1870
                     /locus_tag="F8D37_RS28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(2412..2858)
                     /locus_tag="F8D37_RS28460"
     CDS             complement(2412..2858)
                     /locus_tag="F8D37_RS28460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(3645..4055)
                     /locus_tag="F8D37_RS28465"
     CDS             complement(3645..4055)
                     /locus_tag="F8D37_RS28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4055..4246)
                     /locus_tag="F8D37_RS28470"
     CDS             complement(4055..4246)
                     /locus_tag="F8D37_RS28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5..265
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.947"
                     /coverage="92.0"
                     /evalue="8.16e-121"
     Typing          4366..4510
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.83e-70"
ORIGIN
        1 tatcaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc cattcggttt
       61 accggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgccaga cactcagttc
      121 cgggaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg
      181 ctgcgtctta tccggtaact atcgtcttga gtccaacccg gtaagacatg caaaagcgcc
      241 actggcagca gccactggta actgatttag aggagttagt cttgaagtca tgcgcctgtt
      301 aaggctaaac tgaaagaaca agttttggtg actgcgctcc tccaagccag ttaccttggt
      361 tcaatcacct gcaagtgttt gaaccttcgg aaaaaccgcc ctgcaaggcg gtttttccgt
      421 ttacagagca agagattacg cgtaccgaaa ccggatctca agtgctcgta aaagaaaatg
      481 atgatgacct ttagcggtta caaaaaaaga aggcacacta ttacagaggc cttcttctag
      541 tttggttaaa tttttttaaa aaaatcatgc gaccgcatgt tttttttcca aagattcagc
      601 attgtgcttc ctttctataa tttcatgagc gggagtgact ctttctattt cttcctgaag
      661 gcgtgggctg ctcgtaacct cccacaaatc aacagactgc tgtatattta accagaactt
      721 agcggtagta tcgaatgctt tagctaaccg aaacgccata tcggtagaca gtttcctatt
      781 attgttaacc aaagcacttg cagaatttct atgcacgttt aacatttcag ccaagtcatt
      841 gatttttaaa ccacttggct cgagatattc ataaagaata atatctccta cagtcgatgg
      901 ttttctggtt gcttgggaca tgctttactc ctgaattggt cgcttctctc tgttagatga
      961 ggaagataag tatttcttct gttaccagct gtatcgatta gtaattatct taaatatagt
     1021 tacgtcgcgt tactttatta atcattattt gtgatgacgt tttaacgtag gtgaggaaat
     1081 gcctcaccta cttgttttgt aagataacag ttccggtctg ttacctatcg gtaatcatgt
     1141 ggatcgagat acaaatcctc gactccgttc tcaccccact taaatatcaa gcggtactta
     1201 tcattcaccc ttatagatga ataccctgtc aatggagggt tgagattttc aaatcggttc
     1261 cccgggggag accgtaaatc tccaatttta tcagcagcat tcagcatatc cagcttgcga
     1321 cctaatgaaa cactaatcgt agctggaatt tttcgattgg ctttaccgta tttataaaac
     1381 tggtatagcc aatcgtctcg gaactgaatg cggcaatcat catcactatc atgattttgc
     1441 tgcatttttc ttttacctca aatgttgccg caacttggca agttctagat caccttttac
     1501 caccaccata gtacaacaaa cgccagaaat gcacaatagc actgtgcact tgtgcaaaaa
     1561 accataatga tataaaatgc acaaaagcac agtgctactg tgcaaaaaaa gaatagttgg
     1621 aggtcatatg gcgcttgttc gctcaggaaa taaaaccaag atttttgaag cagatccgaa
     1681 atttaaccca gtttttgata cacaacacaa gcctggcgat acgtgcacgt atgaaggaat
     1741 ttacaaatgc acaggttgcg gtaaagaagt aactatcgca ggagggaaaa cacttccacc
     1801 acaaaatcat catcaacata gtcccggaca gggggacatc cgctggcaac tatttgttta
     1861 tgcagagtga ggtatgaaaa tggcagaccg tagaccactt aacccgcatg tcgtcgtccc
     1921 taaaccagga ggcggaactc agccacgaga tcgtaataat aatggaagag tccgagagaa
     1981 acgttcagat gcgggaaaat caagaaaata agaaaagccg ccaaataggg gtcagtttgg
     2041 atatcggaaa cttttgtacg ttaagcgcat tatttaacca atattgatgc gtaagggatt
     2101 gattttacct cagctacgat cgcttttaag tcaccgctct ttctgtatcg catttaggtt
     2161 atccgattaa ggaaaattca cgggaaacgg cgtatagcgc gggagtacat cattgcgaga
     2221 ggtaagcctg ttcaaaacac aggcttaccg tacaataatt ctctatatcc aaactgaccc
     2281 caagaagtct gacactaacc ccacacaaat cgaatccgtt gcaagatgag acacacaaga
     2341 aaagagctag acgaaggtcg ctccgctccc catggttttt gtatacccac catgggggca
     2401 taggtagttt attaactaac tccaagataa tgcgtgatcg cagccgtgag tacaccacag
     2461 cccacttgtt tagataaatc taaaacccct tgtaggattc tcttagtgtt ccactcacct
     2521 tctgcatcca ttggttgaga tagtgcagca ctcaactggt tcttaatctt ttgcgaagtt
     2581 atatcgttat ctattgagtt gatttcctcc ttggctttct ccaaggaagc ctggagttca
     2641 ccgcgttcta tttttaactt ttcacgataa ctgtctggtg tgtgtagttc gttcccatcc
     2701 gcatcgagat taataacttc gcctcgctca ccaacagtaa caaagctctc tacgtgagac
     2761 agatcatcaa caaccatcac attccttttc attcttaaac cagtcacttt tgcacctgca
     2821 ccgatcttca taaaactaaa gcctgattta tcgttcactc acatcacctc ataagataaa
     2881 aatgttaata acgttttcac gagcaattat ttaccataac gcctcattac gcgcaccgct
     2941 caaacgctct agaaacgatt ctggggcgta atccattaaa tcctacgtct taccccctga
     3001 aaacgcctca aaatcgctcc agggcgtttt acgggcatga attgttatgc accagactga
     3061 ctggtggcgg atttcagcct gaattcaggc cagacaactc aaaacccgca ccaaaccaga
     3121 ctgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc
     3181 cggaaaaacg gttttcagca cgttcaggaa tcgggttcgt cgacggccgg caatcacgtt
     3241 atgcaagtcg ccggacgact caccacacga ttcaccggcg attcgactcg tgacttgact
     3301 ctagagtcgc caaggtcaaa aaaacaccaa aatgacgatt cgactctaga gtcaagtcgt
     3361 cataagttac tgatagtctt aagtgagtcg tcgatgactc tatgacgagt cgtcggcaag
     3421 tcgtacacga atcgccgacg acttggattc accttacaga aagtaatact gcgtaatgta
     3481 ataacccaca actaactgat attaatgaat tatcttcaaa attttaattt tgcgtggggt
     3541 gtgggtattt ttagtggtga ggagcgttca gagatctggc gatttttggg aaggttcagg
     3601 aactccccgg gatggccgga gaggaaaacg gaagcatgac ggagtcagga ttttttccag
     3661 aatttccacc acggggaatc gggtggtgaa gaaggttcgt gcttgtgctc cagaagctgc
     3721 atagcctgac gaagggaatc aatatgctca tctcgagcct taacagcccc tttaagacca
     3781 tcgttttcag cttctagtaa agcaattcta tgttgtaaat catgtaaacc aacgctgtta
     3841 acacctgtag atcctgcgtc actttttaca gtatcgatga caccatcaag agaaccaaaa
     3901 actctaataa gctctgaagt atcaataact ttaatgatct tcccgtgctt tacaacagag
     3961 gtgtaactta acttcccact gttaatcatc ttgtaaattg tggggcgact aaccttaaca
     4021 agccgtgccg cttcactaat agacactctg gccatcagtt accatcctgt aattcatcca
     4081 gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt
     4141 cggcctctgc atcaagatca agctcgttaa gcttctccag caggagcaga gactgattct
     4201 ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaga agcctcagag
     4261 cgtcagtgct cagtgtaaaa ccagtctctt ttccgcttcc tcgctcactc agtcgctacg
     4321 ctcctgccgt tcggctgcgg cgagcggtag gactatgcgt ataaatgctc gagaaaaccg
     4381 agcggacgcg tgatgggcag aaggaaaggc cgcttagcgg ccgtttttcc ataggctccg
     4441 cccccctgac aagcatcata gaaattgacg ctcaaatcag tggtggcgaa acccgacagg
     4501 actataaaga
//
