LOCUS       NZ_CP045681             5640 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_9, complete
            sequence.
ACCESSION   NZ_CP045681
VERSION     NZ_CP045681.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045681.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_9"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            complement(224..550)
                     /locus_tag="F8D37_RS28410"
     CDS             complement(224..550)
                     /locus_tag="F8D37_RS28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(782..2557)
                     /locus_tag="F8D37_RS28415"
     CDS             complement(782..2557)
                     /locus_tag="F8D37_RS28415"
                     /inference="COORDINATES: protein motif:HMM:NF015210.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            complement(2547..3440)
                     /locus_tag="F8D37_RS28420"
     CDS             complement(2547..3440)
                     /locus_tag="F8D37_RS28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            complement(3633..3917)
                     /gene="nadS"
                     /locus_tag="F8D37_RS28425"
     CDS             complement(3633..3917)
                     /gene="nadS"
                     /locus_tag="F8D37_RS28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(3919..4251)
                     /locus_tag="F8D37_RS28430"
     CDS             complement(3919..4251)
                     /locus_tag="F8D37_RS28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4407..4652)
                     /locus_tag="F8D37_RS28435"
     CDS             complement(4407..4652)
                     /locus_tag="F8D37_RS28435"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_306589344.1"
                     /translation="MTKTMTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATS
                     QALTHSDRVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4701..5186)
                     /locus_tag="F8D37_RS28440"
     CDS             complement(4701..5186)
                     /locus_tag="F8D37_RS28440"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     Typing          complement(4316..5640)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.622"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcccattcg tccatcaggc gcgatttctc agcgtctgca atgtccggct cctgaaatga
       61 cagcacgccg gaagtataag cgcgggcaaa gtcgcagccg tcgatcagct cgcgcacatg
      121 ttcagggttg ccgcgcagaa cgctggcctg ttcacgctgc cggtctttac ccagcaggta
      181 atccaccggc ccgctgcccg aacccgcacc ccgtgagtga attttaacga tcacggttct
      241 gctccagcag gtcgccaagc tgccggtcaa ggctggtcag cacgacgagc agcgaggcac
      301 gttcagaagg cgtcagggaa tcagcctggt tcaggcgacg ggcgatctgg ttcaagttat
      361 tgccaatccc gctgacctgc cgcagcagtt cgggcgccac ctcaggcaca cggcgcttgc
      421 gggaggtgtg ttcacccaga ccaagctgac gcagccactc cgcaaggtga gtgcggtcgc
      481 agcgatcaag cagtcgctga tgctcggttt cggtgagccg gattttgatc tctttcgttc
      541 gcttttccat aggctccgct gagaaaaaat tcgcaccacc gtgagaattt ttcgcagtgg
      601 atgctggggg tttcgggggg cggcgagccc cctgaaccag tcacggcgac acctcgtaag
      661 aggggacgct gtgtgtactg gcttatagta tgaagtagta tgtcagattc tatgctatcc
      721 gcaaaaaaac agccctctgc taaagagggc tgttatctga tataggttgt ttagtctcac
      781 atcagagcgt gaaggcagcg ttgagggcgt ttgttatctg ttcttgcctc gtttgggcat
      841 cacttacttc ccaagtattt ttagctgcgt tagcagtgtt aaattgcttc actaactcta
      901 atcgtgatcg agcatattct ggcaatttcc cagctaatgg catattttca cagtctctat
      961 ttatatcgaa acaaagcggc agaaggttac ccagcttacc aacataagtt ggactgaaat
     1021 tcgactgaga gcctatatgt tcaatcgaaa ccatatgata tttaagctct ttagttccta
     1081 ctgctagtcg ctctagtgtt tcaaatatgt agataatgag ttttctttga gatagtttat
     1141 cgtccttata ctcgatattt ttcattaagc agtctttaaa caccttttcg cttggtgcct
     1201 ttcttttcag gaaatcaact gtctcatcga ttgtgttttt caatgaagcc ttgctcacag
     1261 ctctatcagc tctgactgca aaaactgaat attttgagtc aatacctgaa ggtctcattc
     1321 gacatacagc attaaattta aaatggaact tctcaaggaa ctctagcgtc tcaataagct
     1381 cagactgttt cataagcctt gatttatctc ttcttgttct gagaagagat aaaatgaaag
     1441 gtcttggaac agtaacgcta aatgtgttga tatttgctaa ggcgtctaat ataggtctct
     1501 ggtgagacag gttccagtat tttgtagatg gagctaccat actcatataa agagatacat
     1561 cattatatat ttcttctaaa aatgaatcag ctgttaacaa gctattttca tttaaaattt
     1621 ctcttttgaa tgatcgatag agctgatctt caccaatata tccatattta gatagccacc
     1681 aacaacggat gtaatcggtc atggtctcgc caactgaacc tagctgttcg tatatgtgaa
     1741 cccatttcct tctggcttca tcttctggga aagttgtagt gcaagactgg aaaaccttat
     1801 tctttataag gtctattgaa ctgaggttaa tacctctggc atttagaact tcaaatatgt
     1861 catatgcatc gtcgcctcga tcaaccgcta tcctgactat gtacagatag ttggttatca
     1921 ttttatagat agcattaaga cataagtcat aatcagcctt cgtaaaacta tcatcatttg
     1981 ttttaaatat ggcgctacat aatgattttt tgcgaagagc ccttttaaag aacgtaacag
     2041 catatcttat tttcttttct tcatcattcg ttgctttggc gtcagactct tccttgcctt
     2101 gaactatttt tttaaaatac tcacctccaa cgctccttgt tagcttttga ataacagact
     2161 caccatcaga atttaaacta tttctatcca gagtgttatt tattgttacg atatattttt
     2221 tatatatatt gtccgcagtt gtgtcatttt tgttctttct aaaatacctt gatattatag
     2281 aaagcatcat ggttattaca gacaatcgtt gctgtccatc cacaacttct aaaacatcat
     2341 cactatcaac acctgatagc acaatggttc caataaagta ctcgtgtaat tttatagtgc
     2401 cgttatcatc catcactata tttcttagaa gatcagccca aagctcctca agctgaattc
     2461 gctcccagct aaactctcgc tgatttcttg ggatctcata tcttttttct acagaaagaa
     2521 tggagttcat attaaatggt gacgcatcaa atctcatttt tttttagttt cctgaagtat
     2581 gtagctctgc tgatgccatg ttctaaccac ggctccccta tagaaggtct tccgcctccc
     2641 ttacttttac taccccgtat tgactggatc tcagaggtat gtgtccgaga aacaaactcc
     2701 ctgaatgaat cttcggaaaa atttttactt gtccatttag ctatgctttt ggcaattcca
     2761 gttacttcgt tatcgtcaag aggcgaagaa aaatgaaggt tataggctgt agcacgttca
     2821 taacaggctt gcagccactg gtcatactca ggccagccct gacggatagc gcggtatgcc
     2881 cacttgcggg ttttgtcgaa cagcgtgcag ttacggccta aaccatagtc ggcaacgatt
     2941 tctttgtcgt tcgccgcgtt caggtcgaga aagtcagcca gccagtccag cgtgtagagt
     3001 tccggctgcc atacggcaat tctccagtgg ctatggttcg gatttttgca gattagcccc
     3061 gaataccccg cgtcagcctc taacttttta cgtagcgcgt tctccacagc ggcagcgtag
     3121 cgcagcggct tcatttttcc gtccggcgcg gtacgtatcg aggttctgag ggcgtacagc
     3181 agatgcgcgt gtccgttgtc ggggttggtg atggtcagcg tgggcgcagg cgcgttccgg
     3241 tcgttccagt cgatggccgc accggtgcga tcgacgtcga atcccagcca gaacatggcg
     3301 tgcggctggt tgaactggat gtatttcgcc agaatggcgc gttccttacc ggcaatgcgt
     3361 acaccaaagt gtaaatcatc cgagaagtac ggcttgtgcg gtaagcggtc gttgaaaagt
     3421 tcgagtactg cgttattcaa agcgccctgc acttatggcg ggctgttgtc tggcacggtt
     3481 catgtgctaa tatcctttca aggtttcgac ctggccctgg ttatcatgtc cgctcgccaa
     3541 agtagagcat gatttccggg gctttgtttt ttctgttgcc gttaaattac acgcgaacgg
     3601 cctcgcctgt cgaatccttc tgtcacaacg gcttagtgcg ccgccagctc ctggaagaat
     3661 ttcgggttcg cctggatggt tgccagcacc ttcgccgcca gtccggtcgg attacggcgt
     3721 ccggtttccc agctcttgat agtgtcgagg ctggtcccca gcactttcgc catctcgctc
     3781 tgagatacgt tcagctgtgc cctgatggct ttcacgtctg ccagctcgta acgggtgacg
     3841 cgggcaggct ctctgacgcc gtttttgatt tcaacggctt cttccagcga tgctttcagc
     3901 tcgtcaaaaa aactcatgtc acacctcgtc tttcagttgt tgggtcagcg acttcagcac
     3961 ggctttctcc gcgtccgtca ggctgtcttt ggtgcttttc gggtaggcca tcaccagata
     4021 aatcacctcg gcggtggcca gaaagtaaat aaccctcgca ctgccgcttt ttccctggtt
     4081 gcccgtcgcc attctgattt tgcgcagccc accggttttc tggatgagat cgcctttatc
     4141 cggtgactca atcagcgtct tttgcagctc cctgagttct tcatccgtgg cgatctgttt
     4201 tatctgacgg gtaaacatcg gggtctcgat aaactcaatc gcgtgactca cggctgtctc
     4261 ctgtgttcaa tggcgtacat agtacactta attttaattt cagaaaagcg gtcacatcga
     4321 ggggccgtaa tagcgggtcg gctttttctg cggctccggc tgctgctcgc gcagcttctc
     4381 cgcacgggcg cggtcgtgct gtgcaatcag cgcatcaagc ggctgacgtt cctgcttaag
     4441 ctctcgacct gcacgcgcaa gctgtccacc tgactgctca agtgctgaca caccctgtcg
     4501 ctgtgcgtca gcgcctgtga ggtagcctcg aacatgtgct gcaagctgct gtagctgtcc
     4561 tgccacgcct gctgctgttg ctcgtagctt gtctgtaagc tctctaaggc gctcagaagc
     4621 tgtttttcca tctcggtcat ggttttggtc atcctcggtg ttaaaaacgc gctgtgtcgt
     4681 ttcagggcgc tctgacgggg ttatttcgcc gtgtatttca ctacggtgtt gtcggtcgtc
     4741 gatgtatagg tttccacgcc gtgcgcctgc ttcggcagca ccaccatgta ggtgtttcgg
     4801 tgcttgtcgt cgctgtagat cgccacgccc gcgccgcttt tcgctttcag gctctccagc
     4861 tgttggctgt agctgtccat ctcgctcagg cgcgaggtga tgatcgtgcc ctgaaaccag
     4921 attatcccgc ccatgatgcc gatcagcagg gcgaagcaga ggaatatcca cgccatcgcc
     4981 gttgagacag aggctctgag catgcccgtc agtctgccgt tctgctcgcg gatagcgttg
     5041 ctgatgctct gcgagcttga ttgcagttcc gcgctgatag acgtctccag cctcgcgaac
     5101 tcgcgcttca cgctctgctc ggtatcctgc gcctgctgct tcgatttctc ctcgaaggcc
     5161 ttcgccaaat ttaaaatctc gctcataaat cattcctttc agccggatat ttcggccgcc
     5221 gtccggatcg gcaatgctga tgctgctttt cgtttcccgc gctacggtca ggccgtagct
     5281 ttccagctgc gtcaccacgt cctgccggct gcggatcacc ccctgctgca tcaggttcat
     5341 caggccggcg gtgattttct ctgccgcctg ctgtttgtcg cggggcaaat cgctggcctg
     5401 cgtcagcgga cggcggtagg tcgggtcatt cgggtcgcgc agccccagcc ggtcgttggt
     5461 cagggtctgc caggcgttga cgcggggacg gtcggcgcgg tcaaagtagg gttgcaggcg
     5521 ttttccactc tgcaattcga tgttcgggat gacaaaattc agctcaagcc gtcccttgtc
     5581 acggtgctcc acccacaggc aggcgtactg gtcgcgatca aggccggtca gcagcgtgtg
//
