LOCUS       NZ_CP045679             9730 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WSD411 plasmid pWSD411_6, complete
            sequence.
ACCESSION   NZ_CP045679
VERSION     NZ_CP045679.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12877715
            Assembly: GCF_009884415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Horizontal gene transfer and evolution of plasmids with
            blaOXA-48-like genes in Klebsiella pneumonia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Chen,Y., Yang,Y. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Department of Intensive Care Unit, Sir Run
            Run Shaw Hospital, College of Medicine, Zhejiang University, No. 3
            Qingchun East Road, Hangzhou, Zhejiang Province, P.R. China,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-OCT-2019
            Assembly Method                   :: CLC Genomic Workbench v. 9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:10:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 117 of 182
            Pseudo Genes (internal stop)      :: 30 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045679.1.
            Bacteria and source DNA available from Yunsong Yu's Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WSD411"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pWSD411_6"
                     /geo_loc_name="China"
                     /lat_lon="30.26 N 120.20 E"
                     /collection_date="2018-08-26"
                     /collected_by="Sir Run Run Shaw Hospital, Zhejiang
                     University School of Medicine (Hangzhou, China)"
     gene            join(9024..9730,1..25)
                     /gene="mobC"
                     /locus_tag="F8D37_RS28315"
     CDS             join(9024..9730,1..25)
                     /gene="mobC"
                     /locus_tag="F8D37_RS28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            32..562
                     /locus_tag="F8D37_RS28320"
     CDS             32..562
                     /locus_tag="F8D37_RS28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(590..769)
                     /locus_tag="F8D37_RS28325"
     CDS             complement(590..769)
                     /locus_tag="F8D37_RS28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            838..1215
                     /locus_tag="F8D37_RS28330"
     CDS             838..1215
                     /locus_tag="F8D37_RS28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(1277..1576)
                     /locus_tag="F8D37_RS28335"
     CDS             complement(1277..1576)
                     /locus_tag="F8D37_RS28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1566..1829)
                     /locus_tag="F8D37_RS28340"
     CDS             complement(1566..1829)
                     /locus_tag="F8D37_RS28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2206..3291
                     /locus_tag="F8D37_RS28345"
     CDS             2206..3291
                     /locus_tag="F8D37_RS28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            3288..5477
                     /locus_tag="F8D37_RS28350"
     CDS             3288..5477
                     /locus_tag="F8D37_RS28350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            7063..9024
                     /locus_tag="F8D37_RS28355"
     CDS             7063..9024
                     /locus_tag="F8D37_RS28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          complement(5683..6263)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9025..9728
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.745"
                     /coverage="97.0"
                     /evalue="1.6999999999999998e-137"
ORIGIN
        1 ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgaaga
       61 cgcggaggac cgggacggcg agcgctgcat catgccgacc agcgagcatc ttgaagatat
      121 ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca
      181 tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc
      241 cacgtacttt ttcaccggta aaaatccgca atgggaacgg gacaaacccg caggaatgaa
      301 gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
      361 ggttgatgcc gggaagtgct caccggagca gattagcgcg atgattgagg tggtcagcga
      421 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
      481 cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg
      541 gtggccgttc ggcaaaaaat aaacggcagc accggccatg ccggtgctgt tattccttcc
      601 cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgcagc cgctcgcaca
      661 tgtcggccgg ggtatccata tccagctcgt tcagtttctc gagtaacagc agcgactggt
      721 ccttaataaa tttagccata ttgagggcag ttttttcctg cttagtcatg ttttctgtcc
      781 ttccgggtgt ggtgtgcttc ctgtcgggta tactgtctca aaaccggagg tgagacaatg
      841 gcgtgggtga cggtgagaca ggcgacggaa ctgacgggga aagcccggag ttccctgtat
      901 agggatatgg cgaaaggccg cgtatcatac cgtactgaag ctgatggtgg tcgggtggtg
      961 gataccagcg aacttatccg ggtgtacggt gaattaagac agattgagac tcacgagcgg
     1021 gacgggttga gactgtcgga tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag
     1081 ataaaagcac tcagggagga ggtggccgga ctgcggcagg aaatgcaggc catgagactg
     1141 ctggaacaca aaccagagac agcagagaca ccggccggaa catcgcgccg atggtggcag
     1201 tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga cggccacgca atgaattgcg
     1261 cccccatcac cggttatcag cgaacggtat ggggattctg gcgctggcca accacggcca
     1321 gaacccggac tttcctggtc tggtcatcaa catcatagag gacatgctgt cccatcagac
     1381 tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atataggcgc gggaagtcgg
     1441 ccagtttgtc cgctgcagcg agaactcgat cggtaacgtt ctgcgcatcc cacagggatg
     1501 aattttccgc aatgtagtca aacaatgctg tcacatcctc gatggctccc tgcttccatt
     1561 ccacctcata cggcatcgcg tagttcctga tagcggccat gcagtctctg gcggagttgt
     1621 tccgtggtca tgcccgggcg cgtgtcagcg cgggcggcgg tcactttagc gcgaacctga
     1681 tcttcatatt ctgcctgggc cagttcttcg ttagccaggc gctgcaacag tgcagctttg
     1741 tccgggccac cttcaggcag tatgcggggc agtctctcgg ccagttcccg caggaaattg
     1801 gcgttttcat agtgtgtact gttgctcatt gtggagcctc ccgggctgat acgtcgatat
     1861 gaaggagttt agcagggggc ggtctgaaag aatatgctcg tgtcagtcag gctgatgaag
     1921 acgaaagctg gtaaaaaagg gcttacgaca cccattttac gtcaaactgc acgacatgca
     1981 gttgcgtgca tagccatgca gcgggttaaa atttatcagg cgcgtttccg gcggttttcc
     2041 gggtggtttg ttgccggttt tatcccgtag ccgccggaaa tgccctcaga ccgtctgagc
     2101 ggtgcgcgta atgcgacgtt atggtaaatc atgtgattaa tggtagtgat aatcattatc
     2161 acttttgcta tattcttgtt tcttttattg taaggaaatg taagtatggc aatcacgatt
     2221 aaatccgccg ttaatgtccg tagcagagta ttaatatact ctttactact gtttactggt
     2281 tcagcgtatg cggctgatat agctgaatca acattcctga ctcgtgatat cggcaatggt
     2341 ctgtatgaaa tgtcttttga tggcaaaaat aatagtctct gggcagcatc atcgtcttca
     2401 ttcgacagag ataagggcca cggaattgtt tatcagttag aaccatccac tctgaaaaca
     2461 aaatctgccg ttaaaatcgc acgtcttggg tttgccacag cactggatga ggaacatgag
     2521 cgactttata tcggcaatac tctggacggc tcagtgacgg tcattgatac tctaaccgga
     2581 aaagaacttt cagtaattcc gttaactgag ggacaggatg aggctcattt cagaccgacc
     2641 agagaaatgg tcattgacaa aaaaaatcag cgtctttatg tctccagcgc cgcagataaa
     2701 ggggctctgt gggttattga catggctgtc gggaagaaaa ttaaaaccat tgatggactg
     2761 gggcacgctg ttactggaat tgcgttaaat aaaaatgggg ataatattta tctggttaac
     2821 ggggatggtg agattattaa cctggatgcc agtagctatg aaataaaaaa acggtttgtt
     2881 gtagagcctg agaaaaaaca ctttttcctt aatgtaagtc tggatgaaaa aaacggacgt
     2941 gcatttatta ccgacccgga tattcccggg gtgctggttg ttgatgtaaa ctcgggaaaa
     3001 atactacaca gaattgatgt tataaattca ctggcgattt tatttaatcc tttacggaac
     3061 gaaatctata tcacgcaccg taatgcaaaa caggtcagca ttgtggacag taattcttat
     3121 tacgtaaaaa caacgataaa aacccgcctg atgccaaaca gtctgtcttt atcacatgac
     3181 ggtgaaaatc tgtacgttag tgtaaaacaa gaaaaaaaag agatgagtac tatgaaggat
     3241 tacatactca aaatcgaatt atctaaaatt gctatggagc cagaagaatg aataaatatt
     3301 tagggctgag catagtatat atgatgtata catcatctgt tatggctgat gattctggcg
     3361 cagttagtaa agataaaaat atttcaccag aagaaacatt aattgttcat gggaccgctg
     3421 tttctgaaag cacaacagaa ggaacagatt catacacaac ccagtcagcc aatacagcat
     3481 caaagatgaa tctgtcattc cgggatacac ctcagtcaac tacagtcatt acgcgacgaa
     3541 gaatggatga tgtagcagca aaaaatatta ttgatgtggt taaaaccgct cctggattat
     3601 atgtcagtac agcagaaggt tccggaagac cgaattttat gtccagagga tttaatgcta
     3661 atgtcatgta tgaaggattt agcctgccgt ggaattcata cattccaagt tcacaaacta
     3721 gttcagctat ttttgaccgg gtggagatag tccgcggggc aacaggtctt gttcagggtg
     3781 ccggagaccc ttcagccgcg ataaatatga tacacaagcg tccgacccgg caatatcagg
     3841 ccagcactaa catcggtgcc ggacggtgga atgactaccg gggtggggtt gatgtatcag
     3901 gaccactgaa tgacagcgcg accttacgcg gaagggcagt tgtggatggc gaaaacagtg
     3961 agaccttcag ggatagtgag aaacgcagga ataacgtcat ttacggaatt atggaggcag
     4021 atctgactga taatacccta cttacgattg ggggatatca gcagtatgaa tataacaatc
     4081 attggtggta tgacttacct atttctggta ccggacacca ccttaatctt cctcgctcga
     4141 cgtttacagg caatgactgg gagtttgaaa gaagtaaaag taaaactgca tttgcaactc
     4201 ttgagcagaa gttcagtaat agctggaagc tgaaattttc aacgctgcaa aactggagaa
     4261 atgctgacct gttagggacg gcaacatacc ggcataaaga tacccgacaa agtatgtatc
     4321 agcaagtatg gggatcactg agggaatata attccaaaag ctatgacctg tcgcttaatg
     4381 gggactatgc tttatttggt cgtgttcatc aattcgtggc tggaacaact cagcaaaaat
     4441 acaccgataa agatacaggt tatacatatt ccccaatata tattgagaat gttaatatat
     4501 ttgatcatgg ttcacattct tcgaaaaaac ccatgaagat gtcaggtgat gcatcctacg
     4561 acgtcactga acaaaaaagc gcctacgcag caactcattt tctacttagt gataactgga
     4621 aaatattgct gggtggccgt cttgactggt atgaatatac aagtaaaggt acatccatta
     4681 atacaggtta taaagttacc agaaatatca ccaaatatgc aggttcaaca tacaatcttg
     4741 acgatcatca taccatttat gggagctata ccgatatatt caatccacag tcaagcaccg
     4801 acctcaatgg taaagtgatc aagcctattg ttggtaagaa ttatgagaca ggtattaagg
     4861 gtgagtattt taacggcttg cttaatgcct ctctggcggt atttcaggta gaccaggaaa
     4921 atcgagccag gctgcttccc gatcagacag ggtgtgttac taatggcaat cctcaatgct
     4981 atgaggctag cggtaaagta cgcacaaaag ggatagattt cgaacttcag ggcatggtta
     5041 cgccactctg ggaaattggg gtgggatata catatgcaga tacgcgatat ataaaagatg
     5101 gagatcgtag taatgaaggc aagcgattca atactgccgt gtcgccacaa aatttattta
     5161 agatttcgag catgtacacc tttgacggaa aattaacaaa ctggcgagca ggagtaaaca
     5221 cgtactggca aagtaaaata tatgctaaca taacagacac aagggatggc aatccggtta
     5281 ttaataaaca aaaggcttat tcaacaactg acattattat tggctatact ccaatgccac
     5341 aactggattt acagctcaat ataactaatg tctttgataa ggtttattat acatccatag
     5401 gtgaatcgat tggatatggc acggaaacat atggagaacc aagaaatgtt aaaattagtg
     5461 caacctatag attctaacca tgcggattgg gtcttctcct cggatttgcc cctataaatc
     5521 cgtcttttgt ggttccggcg tacaaccccc tgtttgccac ttcagttgat ggttaatcaa
     5581 atcatgtaga ggatcttctt gagatcctcc atggttcgtc gtaatctttt gccctgtaaa
     5641 cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg gaactgctta
     5701 accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca gtgtagccgg
     5761 actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc
     5821 ccagttacca atggctgctg ccagtggcgt ttttgcgtgt ctttccgggt tggactcaag
     5881 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc
     5941 cagcttggag cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc
     6001 gccacacttc ccgtaaggga gaaaggcgga acaggtatcc ggtaaacggc agggtcggaa
     6061 caggagagcg caagagggag cgacccgccg gaaacggtgg ggatctttaa gtcctgtcgg
     6121 gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa
     6181 aagcgtctgt ttatgtgaat tctggcagga gggcgaagcc tatggaaaaa cgccaccggc
     6241 gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta
     6301 actttgcgag agttagcagc gcccgccgca gtctcacggc cggagcgtag cgactgagtg
     6361 agggaggagg cgcaatatcc cccctcccgc agacggccac cggacgcgcg gatgtgacat
     6421 attgcaccgt gttaccgcct ttcccttccc tgccgataaa tcattcagat aggtgaagtc
     6481 tgtcggtttt ctgctgaata ttttacgtta acctatctct gttgcttttt tctccttgcg
     6541 gagaaatgca ggtcatcccc gctggggcgt ggctgacttc aggaggaaat cccctgctta
     6601 cgctggctgg acgccaccga cgcacggtct ttccccctct ttctggctca gctcggctgg
     6661 acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga ccacccacaa
     6721 aaacccacgg tgctgaacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg
     6781 gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg
     6841 gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc
     6901 ttttccgctt ggggcggaaa gaggctggcc tccgtggtga aagctgaatg caatcaccac
     6961 ggacgtgacc gccgccgcca tgcctcgggg catgtccgcg gcttgtgcag caggggctgc
     7021 acaccacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg
     7081 cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg
     7141 ctgtacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct gtttttcggt
     7201 ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa ggagcgacgg
     7261 gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc ggtgctggct
     7321 tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa ctacgcgtac
     7381 tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     7441 gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga ttattacctg
     7501 ctgcagccgg ctaacctgtt cgggattgtc tggtgtgccg gagggcttgt cgccggcatc
     7561 cttctggcgc ggctgctggc atttctgctg cttgacggtc attttgcggc cgctgacgag
     7621 gcggtgaatg cgaaactgaa ccaggagagc cggcgcagca gccagcggac cggagaaatg
     7681 accgacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt
     7741 tccacagagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag
     7801 ccggtgctgg tcccgcgtga tacctggcgc aagaccaata ttcagatcct cggcctgccc
     7861 gggagcggca agagcgtgat gggcaccaac gcgctcattc gctgcgtacg cgatttcggg
     7921 gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg
     7981 cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     8041 tttcgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt
     8101 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     8161 gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc atacgcgctg
     8221 ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac
     8281 ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa cggcggcggc
     8341 tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg
     8401 tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg
     8461 agcatcatgc tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa cgcgctgggg
     8521 acgctgcgca gtcgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggg
     8581 cagtgcggcc aggatctgcc cgctgagttt gtgaaaacca cggtgctgga taacacgccg
     8641 atccgctggt tctaccgggc ggccagccag gagtccgcgc agtgggcggc gggccagacc
     8701 ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag
     8761 catatcagcg gggacagctt cattcagaag gaggcgcggc cgctgtttga cgtgaacacc
     8821 ctgcagcacc tgcctgacgg gtttgcggtg atgaccgggc tgggcgtggc ccggatggcg
     8881 ttcagcagcc cgctgcgggt tgaacgccgg gaaatcccgc tgcagcgctt cccggtgctg
     8941 gcgaaaacgg acccgctggc ggagtaccag ccggaggccg ggcggcagcg gccgggtgat
     9001 gatgattttg cggggctgta ctgatggcac tggagcgata caacgtcagc cacgcgaagc
     9061 ggcaggcgcg gaacgcggag aaaacccggc tgaccctgcg ctggctgcgg gaagagctgt
     9121 gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtgcag ccggtttacc
     9181 gcttcctgga cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg
     9241 gccgtcaggt cagcgtgtgg ggactgacgc cgcacggggt ggcgttctcg ttcgacgagg
     9301 acgaaccgct gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc
     9361 cgcaccggct ggccgtgcag tccctgcgtc tcgccatgga ggcccggggg gcctccggct
     9421 ggcggtacct gcaccgtatg gccctgaagg ggatgaaggt gccggacgcg ctggcggagc
     9481 tggacggcag aacggtggcg tttgaggtgg agcgcaccgt gaagagccgc aggcgctatc
     9541 aggaggtggt gtccgggtat ctgttcaatc gcaaagcgaa cggcatcgat gaaatctggt
     9601 acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcgatcctg tcggtggatg
     9661 aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggc ggagctggac cgggagcggc
     9721 tgtttgcgtg
//
