LOCUS       NZ_CP045647            13568 bp    DNA     circular CON 22-JAN-2024
DEFINITION  Staphylococcus epidermidis strain IRL01 plasmid pBPH0747-01,
            complete sequence.
ACCESSION   NZ_CP045647
VERSION     NZ_CP045647.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09103952
            Assembly: GCF_009685135.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Lee,J.Y.H.
  TITLE     Mining the methylome reveals extensive diversity in Staphylococcus
            epidermidis restriction modification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13568)
  AUTHORS   Lee,J.Y.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2019) Microbiology & Immunology, University of
            Melbourne, 792 Elizabeth Street, Melbourne, VIC 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/22/2024 01:54:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,702
            CDSs (total)                      :: 2,621
            Genes (coding)                    :: 2,542
            CDSs (with protein)               :: 2,542
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 47 of 79
            Pseudo Genes (incomplete)         :: 46 of 79
            Pseudo Genes (internal stop)      :: 23 of 79
            Pseudo Genes (multiple problems)  :: 28 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045647.1.
            Bacteria and source DNA available from Jean YH Lee, University of
            Melbourne.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="IRL01"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="pBPH0747-01"
                     /geo_loc_name="Ireland"
                     /collection_date="2016"
     gene            1..936
                     /locus_tag="GFV05_RS00005"
                     /old_locus_tag="GFV05_00005"
     CDS             1..936
                     /locus_tag="GFV05_RS00005"
                     /old_locus_tag="GFV05_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(1161..1400)
                     /locus_tag="GFV05_RS00010"
                     /old_locus_tag="GFV05_00010"
     CDS             complement(1161..1400)
                     /locus_tag="GFV05_RS00010"
                     /old_locus_tag="GFV05_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1417..1686)
                     /locus_tag="GFV05_RS00015"
                     /old_locus_tag="GFV05_00015"
     CDS             complement(1417..1686)
                     /locus_tag="GFV05_RS00015"
                     /old_locus_tag="GFV05_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            1895..3817
                     /locus_tag="GFV05_RS00020"
                     /old_locus_tag="GFV05_00020"
     CDS             1895..3817
                     /locus_tag="GFV05_RS00020"
                     /old_locus_tag="GFV05_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            4091..4504
                     /locus_tag="GFV05_RS14170"
                     /old_locus_tag="GFV05_00025"
     CDS             4091..4504
                     /locus_tag="GFV05_RS14170"
                     /old_locus_tag="GFV05_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            4581..4814
                     /locus_tag="GFV05_RS00030"
                     /old_locus_tag="GFV05_00030"
     CDS             4581..4814
                     /locus_tag="GFV05_RS00030"
                     /old_locus_tag="GFV05_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(4863..5567)
                     /locus_tag="GFV05_RS00035"
                     /old_locus_tag="GFV05_00035"
     CDS             complement(4863..5567)
                     /locus_tag="GFV05_RS00035"
                     /old_locus_tag="GFV05_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5718..6416)
                     /locus_tag="GFV05_RS00040"
                     /old_locus_tag="GFV05_00040"
     CDS             complement(5718..6416)
                     /locus_tag="GFV05_RS00040"
                     /old_locus_tag="GFV05_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            6580..6678
                     /locus_tag="GFV05_RS00045"
                     /old_locus_tag="GFV05_00045"
     CDS             6580..6678
                     /locus_tag="GFV05_RS00045"
                     /old_locus_tag="GFV05_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6855..7766)
                     /locus_tag="GFV05_RS00050"
                     /old_locus_tag="GFV05_00050"
     CDS             complement(6855..7766)
                     /locus_tag="GFV05_RS00050"
                     /old_locus_tag="GFV05_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(8185..8745)
                     /locus_tag="GFV05_RS00055"
                     /old_locus_tag="GFV05_00055"
     CDS             complement(8185..8745)
                     /locus_tag="GFV05_RS00055"
                     /old_locus_tag="GFV05_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(8996..9562)
                     /gene="qacR"
                     /locus_tag="GFV05_RS00060"
                     /old_locus_tag="GFV05_00060"
     ARG             complement(9000..9562)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9740..11284
                     /locus_tag="GFV05_RS00065"
                     /old_locus_tag="GFV05_00065"
     CDS             9740..11284
                     /locus_tag="GFV05_RS00065"
                     /old_locus_tag="GFV05_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="WP_000622776.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            11489..12049
                     /locus_tag="GFV05_RS00070"
                     /old_locus_tag="GFV05_00070"
     CDS             11489..12049
                     /locus_tag="GFV05_RS00070"
                     /old_locus_tag="GFV05_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(12368..13129)
                     /locus_tag="GFV05_RS00080"
                     /old_locus_tag="GFV05_00080"
     CDS             complement(12368..13129)
                     /locus_tag="GFV05_RS00080"
                     /old_locus_tag="GFV05_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             9741..11281
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..936
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1896..3748
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgcccaatt ttgaaaaata taatttatca caagtaaaaa ctgaaagatt ttatcaactg
       61 cctaaatatt tatttgaaga tgcatatttt aagaaaatgt ctgcagaagc caaaattatg
      121 tatgcgttat taaaagatcg ttttgaatta tccctccaaa atgaatgggt agataaaaat
      181 aataatattt actttatttt cagtaataaa catttgtgtg aatacttagg ttatgcagaa
      241 caaaaaatta taaaattaaa aaaagagtta ataaaattta atttactaac tcaagaacgt
      301 gttggcctta ataaaccaaa tagattatac ctattaaaac ctaattatga cattgaagcc
      361 agtcatatca aggaacttcc aaattcacag ttccagaaca atgaatttgg aagttctaga
      421 actgtgaatt taagtggtca agaacttcca aattcacagt ctaatgatac tgattataat
      481 gacactgatt atattaagac taattataat gatatgtatg atttgaatga taggaaatta
      541 acatattcta gtaatcatac aaatcattca aatcactact attcaaaatt caatgatgaa
      601 gctttaaaat ttcaattact tgaagaacta ccacaaagta ttcaaagtta tttaagtaac
      661 ttttctgtat ctgaaattaa acttattaaa tctgtgttat taaaagccaa aacatccttc
      721 aacaattcta ttgatgcata ttatttatta gaagatatgg aatttgaaat tgttaatgtt
      781 cttaagcgtt ttaaagctac attaattcaa aaaaatgaaa ccgttgaagc aatgcaaggg
      841 tacttaatga aatctcttaa atctgaattt gcagaagtac atacgcttaa taaacgacgt
      901 gatcatttac caatcacttc tttatttaat caataataca acttaataag ccaacaatga
      961 tccaattaga gaatatcttt aattagatgt tctcttaact gtaagcgaac tttattttca
     1021 atgaaatgta attaattaag taagtgggac taaaactttt cttttttagt cccattttta
     1081 taaacaatta ttattcattg ttactactat cagcaatttt ttctttttag tgaaacccaa
     1141 gtatttgtaa gcatgataaa ttacttcttt ttatatttat tgggtttatt tgtattaatg
     1201 tagttggaat acatctttaa caattcttct gtttcatcag gacttaaatg ctcacattca
     1261 aaatactttt caagcattgc accttttcga atcagtctat gtgctctttc ttttctatct
     1321 aaatttttta atctatgctt tcttaattct gctttttcaa tttcagcatt tatttttttg
     1381 agatgtttaa aatcattcat cgtatcactt actattctag ttaattttgt cttctgttaa
     1441 attttccttg ttctcgttgc tgctattttg ttcgatatct ttattaaatt ctccaatgac
     1501 tttatccata attttataca attcatcact agagttgatc atatttaaat caagtttatt
     1561 taaaaaataa ccaccaattt tattagatag ttgttcttct tgttttttct tttgctcttt
     1621 cagttgtgca atttgttttt ctaattgact taattttgta atatctgttt gtttaatttt
     1681 agacattaaa aaactccctt agtttatcta tataaagcat accataattc attgggtaag
     1741 tcgcacatcc atggtataat ataaccagtt cttaagacac tgcactgccc atttacatgc
     1801 aaattgaaaa ttggcataaa aatgggcaac catactggtt gatcattata gttcccacag
     1861 gggtaaaaaa acagaaaaga gaggaggtaa aatcatgaat aaaggttact ttatgttgag
     1921 agtgactaac attaatcgag caacaaaaaa tatcgttgcg tcagcgtctt atcgttctga
     1981 tgaagcatta tatagcgaac gtacagatga aaaaattaag tttagaaatc atactattaa
     2041 gcctgaaagc atgattttaa ctcctcaaca tgcacccgaa tggactaaag atagacagcg
     2101 tttatggaat gaagttgata aagttgaaaa gaataatgcg aaaacaaaaa atcctagatt
     2161 agcaaaagaa gtcttattaa gtttgcctaa tgattttgat agagaggtac aaactgaatt
     2221 aactaaagat tttataaaaa gtgagtttgt agacaaagga atggttgctg atatttcaat
     2281 tcatagagat gatataaaca acccacacgc ccatgttcta cttactcaaa gaccgtttaa
     2341 tgcagacggt acttggggga aaaagacaaa aacgagaacg caatatgatg aaaatggcaa
     2401 tgcgatttta aataaaaatg gtaataaagt tagaaaacaa gaacgatttg ccgactttga
     2461 tttcaaagaa gttcgtaagc attgggaatt aaaattaaat caatactcag aacgtgaaca
     2521 aagtttaaga cgatatgata gtcgttcttt tgaagcgcaa ggtttagaaa aaattgccga
     2581 aattccactt acaagagaag aatatagatt agaacaaaat gaaagaaaac gctgtgaaaa
     2641 agagggtatc gagtaccaac ccgttactta ttacggacag aaaaatgaac aaattagaag
     2701 atataatcgt ggcgaagtat cacaattatt tagtgataaa gagaaagaac aagcacaaaa
     2761 tgaaattaga ttatttattc aaaatgctaa cgaacaagta agtaaaaatg aacaaagtta
     2821 ttctatttta tttgatagat atcaacgtga tgttggctat tcagaggcga aagaaacaat
     2881 taaaaatacg tttgacagtg cgtctacctt tggtcgtaaa attcaaaatg atttacttaa
     2941 aaatgaaatt aagagacgtc aattaaaatt ctatgctaaa aattatgatg aaaattatca
     3001 gtttagaaaa gaaattgaaa gtaaaggata tagtcaacaa gagtttgtta atactgttga
     3061 aactaactat aaagcgttac tcaatgagaa gaataaatta gaaagtagag aggaaaaacg
     3121 taaagagatt tatcaatcag ctaaagatgt ttatctaact gaaagtgaaa aaaatcatct
     3181 cattgttcag caaatgtacc ctaaatctca tcatcatttt agccatgatg aaaaagcatt
     3241 tattgttgat gaggcacaaa aaggtaatat tataaaagca actcatgttc aatacctgtt
     3301 caaacaaaat aacaatattc ctaatgatgt attgctagaa gatacttata aaaaagcgag
     3361 taaagatatt ttctttagtc aaaaacatct taaaaattta gaaaaagata gctttgaata
     3421 taacgttgag ttgcaattta ttaaggaaaa agaaaaagaa attgatagtt ataaagaaca
     3481 tattgatgat gatttaagaa tagatatagg cgataaaaac tttgaaaaag tcaaagacca
     3541 aacacctaat gaaaaagttc aattactcat taaacttgag aaatataatc atgtcgatca
     3601 agaagaagtt atcgaaaaac atattaatca agtagataga aatgatcatg acaatcgtat
     3661 tgaacatgaa aatgaacaaa ctaatcacat taatttacca acgataaatg ctagtgactt
     3721 tcttatgtac tatgcgaacg aaactgaaaa gcaaaacaat attaatcatc agaaaaagaa
     3781 gaagaaacaa gagagaaata accaaatgag aagatagtaa gcagttgaaa tcatacaaag
     3841 ttcattcttt aataataaga aacttaaccc aatgtggtaa attagctttg gtttaaagaa
     3901 aacatttata tacttagata aggtacattc tatgtattat cacaatgtat tttaaaacgt
     3961 gtataattat agaaaaacct tgatattatg cgattgttca catatttcag cataaaaaat
     4021 actaatgttc ataaatatcg atagaaaaaa ttgagtttaa atatatctta aagttaagga
     4081 gaaaaataat ttggaaacaa aaattggaca tcaaatacaa aagttaagaa aaagtaaaaa
     4141 catgtctcaa gaaaaattag ctgaaaagtt aggggtatcg agacattcta tttctaattg
     4201 ggaaagagaa gtaagtaatc ccgacttaaa aacaatttta gaaatcacta aattatttaa
     4261 tgtttctcta aatcaattaa ttaaaggagt ggaaatcatg caagttaata aatatgttta
     4321 ctctgcatta gcattttttc taggaggttt cggggctcat aagttttatg ttaagaaaaa
     4381 taaaaatgct cttctttatt tactattttg ttggacggga ataccgggtg tgataggtat
     4441 ggtagaaggt gttttaacac ttatgcgtcc gagcgatagt gaaaacaata tagaaataaa
     4501 ttaaaccaaa aataaaaagc cgatgtatcg gatttttttt agtgtacatg tgaatattaa
     4561 aaatgatgga gtgatttcac atgaacacat tcgttttcgg cgtgattaca aagcgattag
     4621 aagaaatagg ttttaatgta cacaaggtgt ttaaaatgcc cgaaaaacag gcactatttg
     4681 aagatttgta ccattatgtg agatacgtta aaccatgcaa ggctgaacta acaatggtgg
     4741 cattagatag tgatgaattg attcattact ttatatgctg ttacatggaa cgttatgctt
     4801 atcttttgaa ctaatgataa aaaagacgcc taattttagg cgccttttta ataatctact
     4861 aactacttat tatatctaat tttacttgct aacttagcta ctgttttatt gtcgtagggt
     4921 ttaatataga ctgtttcaat attctcctct attgcttttt ttgttatctt attcatttga
     4981 ctataatcac tattttgtga attttcccat ttataaggga tattattata tacattaatt
     5041 gttccatcac cattactgct atatgttaca gacccaccag ctctcatagg agcttctaat
     5101 ttcacaactt tttctttata aattgcacta tttttggctt gtggatttac ttttgagcct
     5161 tttggtattt ctgtaacata aacattttta atacctttta aatcatttct tgtagatatt
     5221 aactgatacc aaactcgtgc atattcaatt tctttcgagt taaaattatt taagtaactt
     5281 ttatttttat cagtagattg ttgcttctta ttactatcat tatttttttc ttgtttttta
     5341 ttatcatttt tttctttatt ttgtttatta ctactttctg aattatccgc actatcagat
     5401 gtgttagttt tttcgtcaat ttcatttgat gcttcattat ctttgttttc tgaggtgtta
     5461 gtatcactag aattattatc attgtgatgt tctgaatcgt tattattaca actcactaaa
     5521 attaccgata aactaacaat taatataggt aaaatgattt ttttcatatt acaactcctt
     5581 ttcagtattt caaattagat tatgttaata ttttttcagc aagaaaacgt aaattacatg
     5641 agttgaaaaa tatgtattaa atatataata aaataaccac atgttttaca taaacacggt
     5701 ggttatttta tcaattttta tattgccttt tttctgaata ttaaaagact tatacctaat
     5761 attagtagtc cagaagcgcc aattattgac aatatatcac tttggttgct ttctcctgtt
     5821 tctggtaata cttgagagtt tgaatcttga tttttcattc cttcagaact cttgttttta
     5881 ttttcagtta caggtgtcaa cacaccttga ggtgtattaa tagttttatt ttcttcatta
     5941 gctatctttt gactttcatt ttttacaatt tgattatatt tttctgtgga tacactatta
     6001 gggttaggaa catgactttc taattcttgg tattcttctt gagtcataca attaggtatc
     6061 acccctccga aaggtctttt accttgttga gttctctcat tattttgtat atcagtactt
     6121 tgtgattgat attcattaaa tacacttgaa actttctcat tatactcgat ttgagttaat
     6181 tcccctgaat ctttctgttt agaaagttca cttatttgat tttgtgcaga ttgtgggagg
     6241 gatgaagtat tgaagtctac attagcccaa ccatctccat ttgtatctgc gctatttggt
     6301 aagttctctc cgtatacggt tttggcacca tcctcagcat tggctgtcgc tgaaaaacct
     6361 gtgaatgtta atgagctagc caataataaa gttggaagta atgatcgttt tttcataaaa
     6421 ttatctcctt taatagaatt tgtattttca ttatcacata gatacattaa acataaaatg
     6481 acttatcgga agtgcatatg cttaacaatt cacaaatgat ataatataaa agtcgtcgtt
     6541 ctacttctgt gctggtttag catggaagga ggtgacacca tgttcagcat catatttgta
     6601 tctatagtag ctcctattat agtaggggtt ataatcacgt tgttttcaca ttggttgaat
     6661 aatcgtgata aacaataaag cgacgacaca gtaataacac caaaaagagc ccgataacta
     6721 ctggaatagt tatcgggctc ggtgtttacc atgttcaaca catatttgtt atctaaatta
     6781 taacatcata gtgtggagga atgcaaaatt gaataattag tatgttaagt ctatactata
     6841 ctgattagta agtactaatt atcatctttg ttattgttac ccctttcatt ggcttttgaa
     6901 tctaattctt tttgaaattt tttgtgtgat tttgaagaag tgtttaaaaa gaaataggtc
     6961 atattatcca tacgatgtcc ttttataaat tcattgataa attgtttttt tgtcattttt
     7021 ttggttgtat tacgttgttt ttgcaatcta atgatttctt cttcaatgta tactcttatc
     7081 atcaaatcta aactagatgt ctgattataa gtagtgaata attttggaac taataaagtt
     7141 ttattaccat caaaaagaaa tggagtgaaa tcataagttt cccatatacc gttagtagag
     7201 aaataataat atgtcacagt ttctttagaa agaaacgatt tcatttctgg aaatgactga
     7261 atcatggatt tattaaaatc aattataaca ttcatgatta tattacttgt taaatctgat
     7321 aatttatcat caccaaaatt tctgattaat ggtgcttttt gaggtattgt taatccgtca
     7381 tcatttaaga taatggaaag aatatcttca ttcataaata gattaatcaa ttcagatgct
     7441 gtagtacctt ttcctgtatt acctttctga gaaaaaccta agcgtaaatg gtgtggttct
     7501 gaaaaatgag agaataattt tctaaattca ttacgctttt gggtatcaga aaaatcacta
     7561 agatgtatat cgataatttt ttcaaaaaaa acatcaattt gctcttgtgc tctttgagcc
     7621 aaagtcgcaa cttttccaac ttctctatta gatattactt taataaggtt tgggtctata
     7681 tattgaagag tgtcttcttc aatagaaata tttaaataat cagttgattt agcatcatga
     7741 ttgaatttgg tacatactag tgtcactcta tatcatcctt ctgtaagttt tttgtttact
     7801 tccaatatag aatgatgtat tattgtaatt gtcgatttac aataagttgt tattctaggt
     7861 ggaagtttat tgcttttgat gtatagatag acttaacctt ttcattatag catactatag
     7921 aagaaaatgc aagcaaaagc atataaacaa caaatgttgt ttatatgctt ttgctttttt
     7981 gttaattgga gaggacaagg tgaatattaa cagtcaaaat ccagttatgt cggttaatga
     8041 atcgatgaaa attttcaaag ctcaagtaat gcttagaaat ttaaagctat taagcgagtg
     8101 gattgttaaa ggggactgaa gttaaagaca ctttttaaga agatttttca attaaagggt
     8161 atattaaaac tttaatttag tactttatat ttttcttgac tctttaataa tcctatatac
     8221 agtagaacga ctcactcctg tttgttcagc aatttcttca ccagacagct tttgttcatc
     8281 atataagaat ttgatctctc ttttcttatg atctggtagt gaaggtcttc caccttttct
     8341 ccctcttgct cttgcagctt ccaaaccttt tttagttcgt tcacttaata aattagcttc
     8401 aagttcagcg aatgcactca tcatggtaaa gaacatcttg cccatcgcat ctttcgttga
     8461 cacattcata tctataatat gtaaatcaat gctattttca tccaaccatt gagatagctc
     8521 aattaattgt tttgttgtac gtccaagacg atctaattta tagacaacta gaatatctcc
     8581 ttcacgtaaa taatctaaac acttatcaag ttccgttctt ttagttttac gaccactcgc
     8641 tttctcgcta aagatacgtt cacaaccata ttcctcaagt gtatcaatct gtccatcaag
     8701 actttgatct tgtgttgaca cacgtgcata accaatttta gccatttgaa caccaccttt
     8761 tttaatcgtt ttatgggtac ttactaatga aatatagttc tcgatgtaaa taaacacgtc
     8821 tgtcagcatc taaaaactca tgattttcga tatttatgat acattgatta taacacaagt
     8881 ttatagactt aaatatccaa gaaaaacctc ttaatattaa gttaagaggt tttgtgtcaa
     8941 aaacgaacgt aatttagaaa taatatttat tggtatttca agaatatata tataattatt
     9001 tactaagtcc atttaaaaaa atttgagaga acttgttcat aagtttaatt ctttcattaa
     9061 tattttgttc atgtgtaaag gtaacaattc cattaacagc gtttgctgct attttactaa
     9121 cagcattaac atcattaata caccattcgc catttaaatt accctcctta aaaattacat
     9181 gataagcatc tatatatttg ttttctaatt tattcatttt ttcattaatg ctattagttt
     9241 tataatattc agtgtaaaat tcaattattg cgttttgaag tggataatag tattcggttg
     9301 ttaaagatag ttcattatat aaataaaatt tttctctatt agttttacat ttgatttgtt
     9361 cctttttcca ctgttcttgc catttagatt cttctatatt taaaatttct aaaaatagat
     9421 tttcttttgt tttaaagtga taataaagat tccctttact actttctgat aatttaacaa
     9481 tttctccagt agtagtggca ttatatccat tttttataaa taattccttt gcgacaccta
     9541 gtattttatc tttcaagttc atcacgcctt taatgagtat taagtactat caatgatagc
     9601 acaaaaaatt caaagaataa ttgtataaca caatacagcc atttaaattt cgcaagattt
     9661 tttgttgtaa tatgtaaaaa aatagattat aatccttata gaccgatcgc acggtctata
     9721 aggattggag ggaacttaaa tgatttcatt ttttacaaaa actactgata tgatgacatc
     9781 aaaaaaaaga tggactgcac tagtagtatt agctgttagt ttgtttgttg ttacaatgga
     9841 tatgacaata ttaattatgg ctttaccgga attagtaaga gagttagagc cttctggtac
     9901 ccaacagtta tggatagttg atatatactc tcttgtttta gctggcttta taattccatt
     9961 gagtgccttt gctgataaat ggggaagaaa aaaagcatta ttaactggat ttgctttatt
    10021 tggcctcgtt tcattagcta tatttttcgc agaaagtgca gagttcgtaa tagctattcg
    10081 atttttactt ggtattgcag gtgctttaat aatgccaact actctttcaa tgataagagt
    10141 aatttttgaa aaccctaaag aaagggccac tgcattagct gtatggtcaa tcgcttcatc
    10201 gataggtgct gtttttggac caattatcgg aggagcttta cttgagcaat tttcatggca
    10261 ctcggcattt ttaattaatg taccgtttgc gataatagca gttgtagcag gtttattttt
    10321 attaccagag tctaagttat caaaagaaaa gtctcactcg tgggatattc cttctacaat
    10381 tttatcaatt gcaggcatga ttggactggt atggagtatc aaagaatttt caaaagaagg
    10441 actagcagat attattccat gggttgtaat agtattagca attaccatga tagtgatatt
    10501 tgttaaacgt aatttatcaa gttctgatcc aatgttagac gtaagacttt ttaaaaagag
    10561 atcattttca gctggtacaa ttgctgcatt tatgacaatg tttgcaatgg catctgtttt
    10621 gttattagct tcacaatggt tacaggttgt ggaagaactt tctcctttta aagctggctt
    10681 atacctatta cctatggcaa taggagatat ggtgtttgca ccaattgcac ccggattagc
    10741 ggcgcgattt ggaccgaaaa tagtgttacc ttccggaatt ggaattgcag ccattggcat
    10801 gtttattatg tatttctttg gtcatccatt atcatattct acaatggctt tagcattaat
    10861 tttagttgga gctggtatgg cttcactagc agttgcatct gctctaataa tgttagaaac
    10921 acctacatca aaagcaggta atgcagctgc tgttgaagag tctatgtatg accttggaaa
    10981 tgtttttggt gtagcagtac ttggtagcct atcttctatg ctttatcgtg tatttttaga
    11041 tatttcatct ttttcatcaa aaggtatagt tggagattta gctcatgtag ctgaagaatc
    11101 tgtagtgggc gctgtcgaag tagctaaagc tacggggata aaacagcttg caaacgaggc
    11161 tgtaacatca tttaatgatg cttttgtagc aactgcttta gtaggtggga ttatcatgat
    11221 tatcatttca atagttgtct atttgttaat tcccaaatca cttgatataa ctaaacaaaa
    11281 atagtactaa acatatattt ttagatattg tagattagtt tatttttaaa attagtaata
    11341 gttaaaaagt agaaccatta attttaaatg gttctacttt tttattatta agtaaaatta
    11401 taaaagacaa tttcttaaat taataatatg atatacctat atctaataat ttattagtat
    11461 taataaacct tgaaaggatg attaactgat gaatagagca gatattttta aaatatttga
    11521 tgtagaactt aatataacta tatttttagg agcactagta gcaactttca tctattttat
    11581 aagttacatg aacaatgtaa atgaattaat tacattatgc ttaacattag cttttcctgc
    11641 atatttatta aaaattagca gatcgttcga ggttatttta ttagaggaaa aaataaaaaa
    11701 tagtaaaatc aaaatctgtg acaaagatag aattgaacga gagatactgg tgaaaaagaa
    11761 cctaatagat gaactattgt ttcctaatag agttattaaa aaatatacac tgacaatttt
    11821 aatttacagt ataggtatta ttattctttt actactttgt aatatgttaa gttatggaat
    11881 tggtgaaaat gtcaatttta tctggagatt actagcttgt tatgtaacga gtgtcacagt
    11941 gattatagtt gccttgatta taatgataat gatatttgat cttataattg gattactatt
    12001 aaattttcaa atatttttaa aaaataaaaa gtatgatgac tttttataat tattcgattt
    12061 atataatgaa aaattaaatg attaaacata gaaacctcaa aacaaaccat atttaataga
    12121 gtttatggcg ccttatacaa ataaaggcta tgctgttaaa gaattatgta gctatcttca
    12181 gattaatatg gatgatgttt atactattgg cgatagaaaa ttgatttacc tatgtttaat
    12241 accctagata attcttctga taatttagtt tttgttagag tttaacataa ctgatgatta
    12301 tcagaagttt gattcacatt aaaaaggcac aacaagttca tgaacttgtg tgccttttta
    12361 attgcaatta gttaccaaat aaacgactcc aaaatctctt tttaggtggt tctacctccc
    12421 tattatcact ggaatcgttc ttcgtttttt caacaaactt atcctctttt gttaattcat
    12481 ctgtactgtc atgcttattc tcttcagtta tatttttatg atttacttcg gttggttgac
    12541 attctttttg ttgttggttg atattttgag attcttcagt aaaagttgct tcttgtggat
    12601 taatattctg tctattattc atcgttgtat caaaagaata tttaagctgt ctttcttctt
    12661 ctaattggct ttctagtttt tgaatttttt tattgttttc taaagataat atttgttgat
    12721 tctccaacag tttatttaat gtattaacat tgctattttg ttgatctaat tgtttagtta
    12781 gattctcaat atatttttca tcatttttag ctctagtttc aaagatttct atttgctttt
    12841 ctaattcgtt tacttttgtt tttaatgtct cagaactatt tgaattatta tgtatatact
    12901 tttcaacttt tgcagtattt tcattaaata attcattttt tactgattct ttattatgag
    12961 ttgttttctc tttcttagtg attttttcta taatttttaa ataatcatta tctttaatat
    13021 aattaattcc attttctttt tcaaaagata tatttaatct tttagcatta ttaacaatag
    13081 tttgtttagt tacacctaat tcatcagcaa tcattttaat agttttcata acatccccct
    13141 taggtaccta atttagtacc taaattttac cacctaacaa aatttatttc atcatttagt
    13201 actaaattta ctatctatta actacctaaa tgcctaatta tctatcctta aaatattttc
    13261 gccacttaat taggtgctaa aatagcgaat tatacgtttg gtagttttag gtgtactttt
    13321 aattacattt aaaactcttt atatacgcca ttaaaagtgt taatattact ttataaatat
    13381 taaaagagtc gatgctattg gcgtagcatc gactctcggt aataaaacga ttcgcactcg
    13441 tttgtttata tatttttttg atacttgtat tatatatatc taatcatcta agtgcaagca
    13501 caaaacatat aacttacgta aaaattgttt tattacctca atcccaaaat ggaaatgagg
    13561 tttttatt
//
