LOCUS       NZ_CP045615             7195 bp    DNA     circular CON 24-OCT-2023
DEFINITION  Bacteroides xylanisolvens strain funn3 plasmid pFunn3_3, complete
            sequence.
ACCESSION   NZ_CP045615
VERSION     NZ_CP045615.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13118627
            Assembly: GCF_016864215.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides xylanisolvens
  ORGANISM  Bacteroides xylanisolvens
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Sydenham,T.V. and Justesen,U.S.
  TITLE     Sequencing of metronidazole-resistant strain B. xylanisolvens funn3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Sydenham,T.V. and Justesen,U.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2019) Department of Clinical Microbiology, Odense
            University Hospital, J.B. Winsloews Vej 21,2, Odense C 5000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method :: Unicycler v. Unicycler 0.4.7; manual finishing v.
            2019
            Assembly Name                     :: OUH_B_xylani_funn3_2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/24/2023 03:06:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,279
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 93
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 28 of 132
            Pseudo Genes (incomplete)         :: 83 of 132
            Pseudo Genes (internal stop)      :: 41 of 132
            Pseudo Genes (multiple problems)  :: 19 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045615.1.
            Bacteria and source DNA available from Ulrik S. Justesen.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Bacteroides xylanisolvens"
                     /mol_type="genomic DNA"
                     /strain="funn3"
                     /isolation_source="Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:371601"
                     /plasmid="pFunn3_3"
                     /geo_loc_name="Norway:Oslo"
                     /lat_lon="59.9127 N 10.7461 E"
                     /collection_date="2013"
                     /collected_by="Nina Handal and Truls Michael Leegaard"
     gene            complement(join(7179..7195,1..601))
                     /locus_tag="GFH35_RS26655"
     CDS             complement(join(7179..7195,1..601))
                     /locus_tag="GFH35_RS26655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203463008.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAQKLDEYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVTGRLEQSAKIMEQANEGTEAVRQLLQAVKEQTAI
                     QKQLPAPDLLQKATNRVAELEHLIGKYQEKLDSLSEDKTMIIRERVEWERKYYERDKQN
                     YALLETLKKQGELLEKAKTELRHCKGFFSSANEDVLKELGIS"
                     /name="hypothetical protein"
     gene            complement(721..1728)
                     /locus_tag="GFH35_RS26660"
                     /old_locus_tag="GFH35_27400"
     CDS             complement(721..1728)
                     /locus_tag="GFH35_RS26660"
                     /old_locus_tag="GFH35_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_176350731.1"
                     /translation="MAKKEIIAFDPPIEEEIMLIQPNNVTFGQYNVSEWQENLLTLIGD
                     KIQKHMTREEELPRDLFNQPYVVIMCDEAGGKNNKNKVLKEAIDMLNKRFSFRWLHPKM
                     KKEIETTGGSVITNIHNIKGTNQIMVNLNPWAIPFLIYYGVGVGGTRYSKGLALGLRGN
                     YTKRIYKIICSQRDKREYYYAISQFRKDLEIPEKHSNNDIDRKILRPAQERIKESGSDV
                     WFDYELICKYPIKGRKPKADTIIFTINTLHPREAGGEQFEQYSFVYRWVSNAMNNPTDD
                     KAQKAVDKISDLGRLKEVYERCCYYDDRVCSGVQSRIHAQNALLKMLREEFKIK"
                     /name="replication initiation protein"
     gene            complement(2146..2529)
                     /locus_tag="GFH35_RS26665"
                     /old_locus_tag="GFH35_27405"
     CDS             complement(2146..2529)
                     /locus_tag="GFH35_RS26665"
                     /old_locus_tag="GFH35_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="WP_176350730.1"
                     /translation="MKSSEFINWDNLKNIPFAECQVVEDKENKDIDIYYRGEMVFTDYD
                     HVGHYLRNAIALFQTIRRKNADWVNLANLWLLRNCIRENYNHGIGIEDMIFGNDVDTLQ
                     PMTKQRLFAAAKEIQRRDKYVTL"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            complement(2526..2864)
                     /locus_tag="GFH35_RS26670"
                     /old_locus_tag="GFH35_27410"
     CDS             complement(2526..2864)
                     /locus_tag="GFH35_RS26670"
                     /old_locus_tag="GFH35_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070751059.1"
                     /translation="MSLEFVPSDYFATELKRLAKRYRGLADDYEAFLDSLKENPLQGTE
                     IAPKIRKIRMPITAKGRGKSGGARIITYNALVAEQEGKIYLLLIYDKADASNIKMNVVK
                     EIIKDLGL"
                     /name="hypothetical protein"
     gene            complement(2839..3108)
                     /locus_tag="GFH35_RS26675"
                     /old_locus_tag="GFH35_27415"
     CDS             complement(2839..3108)
                     /locus_tag="GFH35_RS26675"
                     /old_locus_tag="GFH35_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_070751057.1"
                     /translation="MIVISTRDFRANQTKFLDMVNNGEDIVLKSREKGSFKLVPVKEED
                     TIIDKRDIIAELKGALQQVKDHMDGKIQLKSAEALLDELRVCSL"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            3784..4302
                     /locus_tag="GFH35_RS26680"
                     /old_locus_tag="GFH35_27420"
     CDS             3784..4302
                     /locus_tag="GFH35_RS26680"
                     /old_locus_tag="GFH35_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_070751053.1"
                     /translation="MQKQTTVTIDQKTFATLDRLAKSNDVSKKEFLSCALEYFEKYGIN
                     PVQHESPAKEMQKLIKRCDQVIAFIRKQEQDILRPACAAISTTEARIERVLDKLATAGQ
                     VRDLQKAVSDQQQRQNYDARNLRSDILQLLGRATEQERKAFMYLGKLVDAKEKSGFFND
                     IATLYKEQG"
                     /name="CopG family transcriptional regulator"
     gene            4309..5079
                     /locus_tag="GFH35_RS26685"
                     /old_locus_tag="GFH35_27425"
     CDS             4309..5079
                     /locus_tag="GFH35_RS26685"
                     /old_locus_tag="GFH35_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_070751051.1"
                     /translation="MNVKIQGGGGGTYANTGSCTGVVTYLQHEDLERIRQGQQAEPFFN
                     QYRDFVSPKEVTYKIDHNRAQLHKTDAKFFVITVSPSEKELAAMGASPEEQARAMKEYI
                     RKDIIPAYAENFNKGLQADNIEYYAKIHYTRTGERLNDMHAHIIVSRKDRANKLKLSPK
                     TNHRGTAKGAVRGGFDRTDFFRKCERCFDVRMNYNREIKESFEYLNAIKNGTTAEIRQQ
                     AIKAVQHEAAREASKEIGKKVTRKIVKGMGGMGL"
                     /name="DUF5712 family protein"
     gene            5091..5543
                     /locus_tag="GFH35_RS26690"
                     /old_locus_tag="GFH35_27430"
     CDS             5091..5543
                     /locus_tag="GFH35_RS26690"
                     /old_locus_tag="GFH35_27430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176350729.1"
                     /translation="MAKKKRKGCLTALLVVWAVFMVKVIAVLNPGFPEAWRMVEAGQIA
                     GFAYIWLLVFLVLLCCFVLWRIVPKGYFRKKKPETSRPAGEWVVSIPTHENPVVVGNPF
                     RGIFIAGAAGSGKSESVAVPLLLEFIRKGFAGIVYDFKFPALDNAF"
                     /name="hypothetical protein"
     gene            5527..5670
                     /locus_tag="GFH35_RS26695"
     CDS             5527..5670
                     /locus_tag="GFH35_RS26695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203463007.1"
                     /translation="MTMHFRQVDGQYRQSVGQQPDSFVSGADIMEYYNRVKEDIKGILG
                     QS"
                     /name="hypothetical protein"
     gene            6248..6553
                     /locus_tag="GFH35_RS26700"
                     /old_locus_tag="GFH35_27435"
     CDS             6248..6553
                     /locus_tag="GFH35_RS26700"
                     /old_locus_tag="GFH35_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032585283.1"
                     /translation="MKKVLFAVALVMGLGSSVAFAGNMVSDVEIVVMVNDYKPIEVKDL
                     PQAVQETIKKDFADLTIKEAAVEESEEGTKTYKVTFTDAEGTDSEVFFNEKGEVLK"
                     /name="hypothetical protein"
     gene            complement(6636..7064)
                     /locus_tag="GFH35_RS26705"
                     /old_locus_tag="GFH35_27440"
     CDS             complement(6636..7064)
                     /locus_tag="GFH35_RS26705"
                     /old_locus_tag="GFH35_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_229084356.1"
                     /translation="MEKKTGLMRLIGYLSFVVVLLYGITLFAVSCDNDEDPPLPPAVEK
                     VVLAFGVDQGEGQLSAKPELENTAAEKIGELISPAEIAKGTTVHFRATHSKSWTIMYWK
                     VNGVIIRSVEPEQTFVIDEHKEVRVAFKPYKSPEPEGL"
                     /name="hypothetical protein"
ORIGIN
        1 cttttagaac atcctcgttt gcagacgaga aaaagccttt acagtgtctt aactctgttt
       61 tcgctttttc caagagttcg ccttgctttt taagtgtttc taaaagggca tagttttgtt
      121 tatcccgttc ataatatttc cgttcccatt ctactctctc tcttataatc attgttttat
      181 cttcgctaag ggaatcaagt ttttcttggt atttccctat aaggtgttcc agttcggcta
      241 ctctgttggt cgctttttgc aatagatccg gcgcagggag ttgtttttgt attgcagtct
      301 gttccttgac agcttggaga agctgccgca ccgcttccgt tccttcattc gcttgttcca
      361 tgatctttgc ggattgttcc aaccgcccgg taaccttctc caatggggag aggtcaaacg
      421 cttcgccccg gagatcgaac ccggttcttt ggaagtaatc aacggataac tcgacaaatt
      481 ctttgcgagt tatacccgta ccgtttaaat actcgtctaa tttttgggcg tactcttggc
      541 ttatcgctat ggtggtaagc ggtgttttcc tcttttgctt gctttcctgt cctttttcca
      601 ttgctataaa gtgctatatg ttgttacaaa ggtagttata tgtttggtta ttgcaattta
      661 ttgcgctttt tattgcgatt tattgcaatt atagcgaaat aaatcagttt tcaaactata
      721 tcatttgatt ttaaattctt cacgtaacat ttttaatagt gcattttggg cgtgtatcct
      781 tgattgtacc ccactgcaca ctctatcatc ataataacaa catctttcgt aaacctcttt
      841 taatcgccct aaatcgctga tcttatccac ggctttttgc gctttgtcat cggtagggtt
      901 attcatggca ttagagaccc atcgataaac aaaagagtat tgctcgaatt gttctccacc
      961 cgcttcacgt ggatgcagcg tattaatggt aaatatgatt gtgtcggctt tgggtttacg
     1021 cccctttata gggtatttgc aaatcaattc gtaatcaaac caaacatcag aaccactttc
     1081 ttttattctc tcttgtgcag gtcttaaaat tttccggtct atatcattat tagagtgctt
     1141 ttctggtatt tctaagtctt ttctaaattg agatatagcg tagtaatatt ctcttttatc
     1201 tcgttggctg caaatgattt tataaatccg tttggtataa ttgccccgaa gcccgagtgc
     1261 aagaccttta gagtatcgag ttccgccgac acctacacca taataaatca gaaaagggat
     1321 agcccaagga ttaagattta ccatgatttg gttagttcct tttatattgt gtatgttggt
     1381 gataacagaa ccgcctgttg tttctatctc ttttttcatt ttgggatgaa gccaacggaa
     1441 cgagaatctt ttattaagca tatcaatggc ttcttttagc actttatttt tattgttctt
     1501 tccccccgct tcatcgcaca tgataactac ataaggctga ttaaacaaat ctcgtggaag
     1561 ttcttcttct cttgtcatgt gcttttgtat tttatcaccg ataagggtta agaggttttc
     1621 ttgccactct gatacgttat actgcccgaa agtcacgtta ttaggctgta tcaacataat
     1681 ttcttcttcg atgggtggat caaatgctat gatttctttc tttgccatac gcttgatttt
     1741 ttaggttacg aagcaaatat aagcataaaa accgactaat aaaaactaaa gtcgtaaaag
     1801 gttgtcggtt ttagtaattt ggtcggcttt tgtgtttgtt attttagtaa tttggtcggc
     1861 ttttgtagcg caattttagt aatttggtcg gcttttgtcc tgtttttgaa cttatattac
     1921 attgtatgtc aatttattac acttgaaaaa tgactttctt acaagaattc ttcaaagaca
     1981 tttctaacaa gaaaggagat tttaggtaat ttgaagggaa aagcgtacaa attactaaaa
     2041 cttcatgcag atgaaacaat agggaaaaga gaacaaggag aaaacagccg agcgcagcgg
     2101 tttagggcag attttccggc gcgttaaaat ttgtaatatg ttgttttata gtgttacata
     2161 tttatctctt ctctgtattt ctttggcggc agcaaacaac cgttgttttg tcatgggctg
     2221 caatgtatca acatcattgc cgaaaatcat atcttcaata cctattccat gattatagtt
     2281 ttcccggatg caattccgga gcaaccaaag attggcaagg tttacccaat cggcattttt
     2341 tcgccttata gtctgaaaca aggcaatagc gttccgcaaa tagtgtccta catgatcgta
     2401 atcggtgaac accatttcgc cccggtaata gatgtctata tctttgtttt ctttgtcctc
     2461 tactacttgg cactctgcaa atgggatatt ctttaggttg tcccaattta taaactccga
     2521 tgatttcata gtcctaaatc tttgatgatt tcttttacaa cgttcatttt aatgttggat
     2581 gcgtccgcct tgtcgtagat cagtaacagg tatattttcc cctcttgttc tgcaacaagg
     2641 gcgttatatg tgataatacg agcaccgccc gacttgcccc gtcctttggc ggtaatgggc
     2701 atccggatct tgcggatctt tggagctatt tccgtcccct gcaatgggtt ttctttcaga
     2761 ctgtctaaga aggcttcgta atcatcggca agccccctgt aacgcttcgc cagccttttt
     2821 agttccgttg cgaaataatc agagggaaca aactctaagc tcatctaaca aggcttctgc
     2881 cgatttgagt tgtatttttc cgtccatgtg atctttgacc tgttgtaagg ctcccttgag
     2941 ttccgctatt atgtcccgtt tgtcgataat ggtatcttct tctttgacag ggacaagttt
     3001 gaaactgccc ttttcccttg actttaaaac gatgtcctcg ccattattca ccatatccaa
     3061 aaacttggtt tggttggctc tgaaatctct tgtactaatt actatcataa tcttctgttt
     3121 ttatggttcg tgtacaaagg taataaaaaa aacgattggt taccaatttg gacgcatttt
     3181 tttgtagata attgcttgtt tggtgtattt tggagtgtac actccaattt ttccgggcgt
     3241 gttctgtgtg tgttccccag ttattgaggg ttcgcccggt ctgtttgtgg aataggttct
     3301 ttcttagtgc tttgcccggt tctccccgtc ccccttgtac gatgcaaaag tacccttttg
     3361 ccgtaccgca ggggagggtg ttcgccggat gcaaagcagc cgaaagaaca gcaagaggaa
     3421 acggcacttt gtaccgtttt tcctcttgcc ctacggggtt acggctgaaa atcagccgtt
     3481 taatgatagc ttagccaatg atttactaat tgatataaaa actaatcttc tgtattacag
     3541 aatgataatt ttatatattg caattatttt agctattatc ggcaggtttt gcctgtgaac
     3601 gaagtgagca agcagtcatt ggacttagtc caatgctgaa tttttatata tctgattttc
     3661 agatgattaa tttttattag tttaccgttg gtttactttt ggtaaaccat atttttgtaa
     3721 ttggctacaa ttcagtatat tgtaccggaa aaataggtaa accaacactt aaaattgaag
     3781 cctatgcaga agcagaccac cgtaacaata gatcaaaaga cctttgccac acttgacagg
     3841 ctggcaaagt ctaacgatgt ttcaaagaaa gagttccttt cctgtgcgtt ggagtatttc
     3901 gagaaatacg gaattaaccc ggtgcaacat gaaagcccgg caaaggagat gcagaagctt
     3961 atcaagcgtt gcgatcaggt gatagcgttc atcaggaagc aggaacagga catcttgcgc
     4021 ccggcttgcg cagcgatcag caccaccgaa gcccgtatcg agagggtgct cgacaagctg
     4081 gcaaccgccg gacaggtgcg ggacttgcag aaagccgttt ccgaccagca gcaacggcag
     4141 aactacgatg cgaggaactt gcgcagcgac atactccagc ttttgggcag agccaccgag
     4201 caggaacgga aagcgttcat gtatcttggc aagctcgttg acgcaaagga gaaaagcggt
     4261 tttttcaacg acattgcgac actgtacaag gagcagggat aagagcctat gaacgtgaag
     4321 atacaagggg gagggggtgg cacgtatgcc aataccggga gttgtaccgg ggtggtcact
     4381 tacttgcagc atgaagatct tgagcgcatc cggcaggggc aacaggcgga gccgttcttc
     4441 aaccagtacc gggatttcgt cagccctaaa gaggtcacct acaagataga ccataaccgg
     4501 gcgcagctcc acaagacgga cgcaaagttt tttgttatca ccgtcagccc gtcagagaag
     4561 gagctcgcag caatgggagc cagcccggag gagcaggcac gagcgatgaa ggagtatatc
     4621 cgtaaggaca tcatcccggc ttatgcggag aacttcaaca aggggttgca agcggacaat
     4681 atcgagtatt acgcaaagat ccactatacc cggacgggag aaaggctgaa cgatatgcac
     4741 gcacatatca tcgtgagccg gaaagaccgg gcgaacaagc tcaaactaag ccctaagacc
     4801 aaccacaggg gaacggcgaa aggggctgta aggggcggtt ttgaccggac ggattttttc
     4861 cggaagtgcg agcggtgttt tgatgtgcgt atgaactaca acagggaaat aaaggagagc
     4921 ttcgagtatc tgaacgcaat caagaacgga actacggcag agatacggca gcaggctata
     4981 aaggcggtac agcatgaagc tgcgagggaa gcgagcaagg agataggcaa gaaagtgacg
     5041 aggaagatcg taaagggtat gggaggcatg ggcttatgaa aaggggagag ttggcgaaaa
     5101 agaaaaggaa aggttgcctt accgccttgc tggtggtgtg ggctgtgttc atggtgaaag
     5161 tcattgcggt gcttaatccc ggctttccgg aggcgtggcg catggtggaa gccgggcaga
     5221 tcgccgggtt cgcttacatt tggctgctcg tgttcctcgt gctgttgtgc tgttttgtcc
     5281 tttggcgcat cgtcccgaaa ggttactttc ggaaaaagaa gccggaaacg tcccgtccgg
     5341 ctggggaatg ggtggtttcg atacccacgc atgaaaaccc ggttgttgtc ggcaatcctt
     5401 ttcggggcat attcatcgca ggggcggcag gatcgggaaa gtccgagagt gtcgcagtgc
     5461 ccttgctcct tgagttcatc cgcaagggat ttgccgggat cgtgtacgat ttcaagttcc
     5521 cggctcttga caatgcattt taggcaggtg gacggacagt acaggcaatc ggtggggcag
     5581 cagccggaca gcttcgtttc gggtgcggac atcatggaat attataaccg ggtgaaagag
     5641 gatataaaag ggattttggg gcagtcgtag cgtgattttt tctattttcc ggcacgaagg
     5701 tagctacgca cgccgcattt gtaaaggggc gtttcactct ccgggtaaaa aatctcctgt
     5761 gctcgcttcg ctcgtgcggt attttttacc cttcgccctt gacaaatact ctgtgcttcg
     5821 ctggcaggtg tttaggaaaa aagaaaaaaa atgttgttct ttggcgggat gtccgctttg
     5881 tcattttgtt accgggattt gtccgcatga tgggagaccc gccccgatca gccggacacg
     5941 caatatcatt ttgtaataac cggctagctg tttttatggt gtaacgctcc gatattgctt
     6001 tctttttgat agatatgcaa atgcaagaag ccccccggcg aaaaccccgc cggaggcaat
     6061 tgtagaaagt ctgtgtatcg tttccacaaa atgtggaaac gatacacaga taacagttct
     6121 tcaataaata tatgtgtttt tgtatgcttt tgattatcag gtatatatga cttgtttatt
     6181 tttttggcac aatgtttgtt ctttgtcttt caggaactat atgtttaata tttaaataga
     6241 ttggattatg aaaaaggtat tatttgcagt agcacttgtt atgggattgg gtagttcagt
     6301 ggcattcgcc ggcaacatgg tatcggatgt tgagattgtg gtaatggtga acgattataa
     6361 gccaatcgag gtaaaggatc tgccgcaggc ggttcaggaa acgatcaaga aggatttcgc
     6421 agacctgaca atcaaggaag ccgcagttga ggaaagcgag gaaggcacaa agacctacaa
     6481 ggtgacattt accgatgcag aaggcacgga cagcgaagtg ttcttcaacg agaagggcga
     6541 agtcctgaaa taaagcaaaa gaacagcaaa aagagaattc aattaaactg tgtcagcaaa
     6601 attaatattt tattgctttg ctgacacagt ttgttttaca gcccctccgg ttccggtgac
     6661 ttgtaaggct tgaaagcaac ccgaacctcc ttatgctcat cgatgacgaa agtctgctcc
     6721 ggctccacag aacgaataat gacgccattg actttccaat acatgattgt ccatgatttg
     6781 gaatgggtag cacgaaaatg caccgttgtg cctttcgcta tttcagccgg agagatcaat
     6841 tctcctatct tttccgcagc cgtattttcc aactccggtt tagccgaaag ctgaccttca
     6901 ccctgatcca caccaaatgc cagcactact ttttccactg cgggaggaag aggaggatct
     6961 tcatcattgt cacaagaaac cgcaaatagc gtaatcccat acaacaaaac aacgacaaaa
     7021 gagagataac cgatcagtct cattaagccg gttttttttt ccatagcagt tttcataagc
     7081 ttattttttg tttaccctac gcaaagataa caatcttgtt ggctaaaagg ggttatgaac
     7141 cttttatttt tttgccctat tatcttaaaa ttcagtgttt atgaaatacc aagtt
//
