LOCUS       NZ_CP045316             2495 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain 14102KSN plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP045316
VERSION     NZ_CP045316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12999969
            Assembly: GCF_016864035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Comparative multi-omics analysis of clinical Enterobacter spp.
            isolates uncovers differences in the expression of determinants for
            antibiotic resistance and virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Medical Microbiology and Infection
            Prevention, University Medical Center Groningen, Hanzeplein 1,
            Groningen 9700RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. 11; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:33:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,457
            CDSs (with protein)               :: 4,457
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 64 of 94
            Pseudo Genes (internal stop)      :: 19 of 94
            Pseudo Genes (multiple problems)  :: 17 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="14102KSN"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="Netherlands: Groningen"
                     /collection_date="2017"
     gene            complement(join(2290..2495,1..22))
                     /locus_tag="GC132_RS23190"
     CDS             complement(join(2290..2495,1..22))
                     /locus_tag="GC132_RS23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1257..1448
                     /locus_tag="GC132_RS23195"
     CDS             1257..1448
                     /locus_tag="GC132_RS23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1739..1924)
                     /locus_tag="GC132_RS23200"
     CDS             complement(1739..1924)
                     /locus_tag="GC132_RS23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1605..1692
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(438..966)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctgaaacaaa aatctttttc aaagccagct ccttttatcc atatcagttt tggtcactaa
       61 ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc tgtatatcga
      121 gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg cttatatgag
      181 atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg tgaggttgga
      241 tgtctaattt ataaaggatc ttcttgagat cctttttttc cgcgcgtaat ctcttgccct
      301 gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttaggtaatc ctggcagatc
      361 ccctaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatttgtcct ttcagtgtag
      421 cctcactaag gccgccactt caagaactct tgagacatct ctcgcacatc ctgtttgcca
      481 atggccgttg ccaatggcga ttagtcgtgt ctttccgggt tggactcaag ttgatagtta
      541 ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc catcctggag
      601 cgaactgcct tcccggaact gagtgtcagg cgtggaatga gaaaccgcgg ccataacagc
      661 ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga gccaccaggg
      721 ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt gagcgtcaga
      781 ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttggc gcggccttat
      841 gctttcttcg ttaagtatct tcctggcatc ccccaggaaa tttctgatcc atccgtaagc
      901 ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg aggaagcgga
      961 atatattctg tatcacattt tctcctgacg cgttttcttt cactttctgc gcctgtctta
     1021 tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat acactccgct
     1081 agtgctccgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt tcagcagccg
     1141 ttccggcctg actgcaattt tttttttttc atccctgccc gctaccctgt aaacctttct
     1201 tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga gccctgatga
     1261 ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg ctacggcgtg
     1321 ccggagggga tgccggagcc atgatgctgg atgcataccg gcagattttg tctgaaaccg
     1381 aaccggccgt aaggactgtc atgctcgaat ttcttgaatc cgttcgcatt cattacattt
     1441 cgtcctgagc attttctgta tttctgtcca tacttaaacg aggcggccgt cacggacggt
     1501 ggtcgtcgtt ccatgatgct gttatggacg agtgagagta atctaatcta acttgattga
     1561 gttccgggcg tgccgcccgg ttttttttgc tgtttcccgg tgtccgttcc gggacaggat
     1621 gtgcatttgt aataccgcgc acgcgccggc atcacaaata ccctggtcag ggctgcgccc
     1681 cgacaccccg ccatcgcctc acggccgcgc actgaattgc gcacccgttc accgattttc
     1741 acgcattttc ctcgcctatc tgtgcgttca gcatggcgta aagcgcttcg gcctgatcgt
     1801 gcaggtgctc acagcagccg gcagcctcat ccagatccat ctgctcgagc cgctcaagga
     1861 ggagaagtgt ctgtgcgcgg ataaatttgg ccatattgag ggcggcggat tcctgtctgg
     1921 tcactggtga ttttccccgt ctggtgatga tttttgcgtg aaaagagcat ggcgtcattc
     1981 tggtgcatag tcatgcatgc cgttaaaatt taacaggcgc gtttctgacg ggttccgggg
     2041 tggtttgttg tggtttttgt catggtttcg tcaggaacgc gctgaacgcg tctcaggcgg
     2101 tgcgggcaac ggatgttatg gtaaattctt gggggcttga ttataaaata ccgcacttcc
     2161 tcttatgtct tgtgcttttt agacaaccct gaaagctttt agctttggtg atgcaatttg
     2221 ctgggatttt ggcggagaac cacaggtaaa gaaaaaggcc acattagcgg cctttttcgg
     2281 agaagatgct cagtgcggaa tttcggagac ttctttaacg tcggttttat tgatctgctg
     2341 tttgacacca ttcgcgtctg tatagccaag caggcctgag tctgattctt taggtttgcc
     2401 gtcgctgaca atagtgcgac cgtcattggt gtgtatggca tagcttgtgc gcgtgcagcc
     2461 ggtgagggct gaaagcgcaa ctgccgctgc gaaca
//
