LOCUS       NZ_CP045315             4666 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain 14102KSN plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP045315
VERSION     NZ_CP045315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12999969
            Assembly: GCF_016864035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Comparative multi-omics analysis of clinical Enterobacter spp.
            isolates uncovers differences in the expression of determinants for
            antibiotic resistance and virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Medical Microbiology and Infection
            Prevention, University Medical Center Groningen, Hanzeplein 1,
            Groningen 9700RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. 11; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:33:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,457
            CDSs (with protein)               :: 4,457
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 64 of 94
            Pseudo Genes (internal stop)      :: 19 of 94
            Pseudo Genes (multiple problems)  :: 17 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="14102KSN"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="Netherlands: Groningen"
                     /collection_date="2017"
     gene            complement(join(4579..4666,1..257))
                     /locus_tag="GC132_RS23160"
     CDS             complement(join(4579..4666,1..257))
                     /locus_tag="GC132_RS23160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_032967811.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     SGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1296..2090)
                     /locus_tag="GC132_RS23425"
     CDS             complement(1296..2090)
                     /locus_tag="GC132_RS23425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_048961857.1"
                     /translation="MKNTAWNVLVYSHADSATTILITYAKSVLVLLIVIIITFSLLSYF
                     IASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRLKKALFTHVKVMMERVDSL
                     NEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFFKKINDSFGHGVGDEVLVS
                     LAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAERIRSVIERTVFPNNLRVTV
                     SAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(2087..2851)
                     /locus_tag="GC132_RS23170"
     CDS             complement(2087..2851)
                     /locus_tag="GC132_RS23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="WP_045619337.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFRGIDIFWVMLI"
                     /name="cache domain-containing protein"
     gene            complement(3197..3412)
                     /locus_tag="GC132_RS23175"
     CDS             complement(3197..3412)
                     /locus_tag="GC132_RS23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3416..3901)
                     /locus_tag="GC132_RS23180"
     CDS             complement(3416..3901)
                     /locus_tag="GC132_RS23180"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3936..4640)
                     /locus_tag="GC132_RS23500"
                     /pseudo=""
     CDS             complement(<3936..4640)
                     /locus_tag="GC132_RS23500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(350..522)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          447..999
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(4..236)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.59"
                     /coverage="98.0"
                     /evalue="7.76e-44"
     Typing          complement(3094..4589)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcaccctgt catggccggt accgccgccg gcgttaacac ggcgggctat ctggttcagg
       61 ttattgccca tgccggcaag ctggcgcagc agcgccggcg agattgaggg aagctttccg
      121 gaacgtgccg gcttttcgtc caggcaggtc tgccgcatcc acgctgccag ctgcctgccg
      181 tcgcagcgct caagcagccg ccggtgctcg tcctccgtca cccacatggt gagcatcttg
      241 ttccgtttct ctgacaaacc ggcctcctga taacgccggg acgggcggga aagcctttcc
      301 cggtcggcgc ggggttggac aagccgcagg cgcgtcaggc ttttggcggg tttcggggcg
      361 cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttacta tgttggcacg
      421 gatgcgagtg tcagtgaaat gcttcatgtg gcaggagaaa aatgcagcag cggagcgtca
      481 gcagaatatg tgatacaaga tatattccgc ttcctcgctc actgactcgc tgcgctcggt
      541 cgttcggctg cggcgagcgg taccggctta caggcggggc ggaaatttcc tgggagatgc
      601 caggaagaag cttaacaggg aagcgataag gccacggcga agccgttttt ccataggctc
      661 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
      721 ggactataaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct
      781 gcctttcggt ttaccggtgt caatccgctg ttatggccgc gtttatctca ttccacgcct
      841 gacactcggt tccgggaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      901 tcagcccgac taccacgttg tcccggtaac tatcatcttg agtccaaccc ggaacagaca
      961 cgaaaaatcg ccagtggcgg tagccattag taagaggatt cacggagcgg agtagtagag
     1021 tgttctacag agttcttgaa gtggtggcct gactgcggct acactggaag gacagttttg
     1081 gtacctgcgc tccgtgaaag ccagttacct tcggaagaaa gagttggtag ctcttgattc
     1141 cggcaaacga accaccgttg gtagcggtgg tttttttgtt tgcagggcat gagattacga
     1201 cgacatcgaa gggataacaa gaggatccgc ttcttgaacc cccctcccca caaaaaaaaa
     1261 cggcacacta gcgcgtaaag cgtcatcaaa aaaactcaaa tagcgctata tacaatcact
     1321 ttattacgcc cctctctctt agcttgataa agagcatcgt cagcattttt cagcaaaaaa
     1381 tcgatcccac caagaggatc atcttgtgtt gccacaccag cagatacggt gacacgcaag
     1441 ttattaggga aaacagtacg ttcaataaca ctcctaatac gctccgcaac acactccgct
     1501 gttaccacag atgtattcgg cagaaaaaca acaaactctt ctccaccaaa ccgacaaata
     1561 atatcttcac tacggcaaca agacttaata atctgagcca aagaaacaag cacctcatct
     1621 ccgaccccat gcccgaaact atcatttatt tttttaaaaa aatcaacatc aatagcaata
     1681 actgaagctc ctgccccata cttgttattc tgtattttag ttgaaaaccc caatctattg
     1741 ttcacgccgg taaggggatc tttaagcgcc acctcattta aagaatcaac cctttccatc
     1801 attaccttaa catgcgtaaa aagtgccttt ttaagtcgat cagcttcggc ataccaagca
     1861 ttgatagctt taatataaac taacgaagcc cctatatctt tagcattggt agatatagca
     1921 agcttttcta aaggaagtga aatacgggag gcaatgaaat atgaaagaag tgaaaatgta
     1981 ataattatta caattagcag aacaagtaca cttttagcat aagtaataag aatagtagtc
     2041 gcgctgtcag catggctata aaccaacaca ttccaagcag tatttttcat atgagcataa
     2101 cccagaagat atctattccc cgaaaaaaaa attccccctc acctctatca gactccgtta
     2161 actttgattt caaatcatca gacataacca tgggccgacc aacagcactg ttatcgttat
     2221 taaagatcac agttccatca tcactaacaa tgctaatttc agtgtcgctc ttgaaaaaat
     2281 gcctgctcaa aacatcgcta aataaactat gtttttttag atatatagac ccaccgacat
     2341 gccctatgta attgccattc ttatcataaa taggatgtga caacaaaata atcaaattcc
     2401 cagtaactga tttgaacggt gaagatatga atggtttctt ttcctcaatc gccaatttgc
     2461 tggcacttga attcaagcgg gtaccaacta aatcaagaga ttctggcgaa gtcgccagaa
     2521 caacagcatc attactaaca acaaaaactg aattaaacat accagactgc aatcttaacc
     2581 tatccgcctc atctttaagt gagtcaatat cattagttga ttttattttt tttgctccat
     2641 atgctaattc accttgagcc atttcaatat aaccactcat tacatctgac attttcatag
     2701 cgtacgatga attagcatca agcaaatctt tttctatatt ttcgcgctga aaaaccataa
     2761 taacggcaat cagaaaaata gaagttaaca aaatcccacc aacggacaag gttatgacta
     2821 tcggacgaag gtgaggcttt ttaattctca taaaaataca ctccatataa taaaaaatgc
     2881 cgcatttacc ataacatccc ttatcagcac cgccccggac gggttcggaa caaatgcggc
     2941 gtcagcccgg taaaaccggc agcaaactct ccgctaaacc gcgcattctg cgccagataa
     3001 atttcacccg gctgcatggc tatgcagtcg ggtgaatggc tccggctttc agcgcagatg
     3061 tccaccgaaa gccccgtttt atcaggttat cacagtgaag ggctgttata cgtccgctgt
     3121 cgctgagatc gctccagctc cagcgctttc tgatgctgca ccacgcgctc ctgctcccgg
     3181 tgcagctcga cggcattcag tcgctcgaca accggcgcag ctgctcgctc aaactctgca
     3241 cctgcctgct caagcccgtg acgcgctcgc tcagcgctgc gttctcccgt tgcataagac
     3301 cagacatcgt ccgccattcc gcgaaggcgc tctcccactc gtccagcctt ttcgagtagt
     3361 cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctccgtc atgtgctatt
     3421 tccccgccag tatcatccag ctcgaatcct ctccgaaccg tcccgcttcc gggttcacct
     3481 tcacgcagcg gcgtctctgc tcgccgcagg tcgagagctg cacgccgctg ttcctctctg
     3541 acagcatggc ctgcgtgctc ttctgctccc ggatggtcgt gtagttgtcg agtatctgct
     3601 gcccctgcca ccacagaatg cccgcgctcg aggcgatcag cagcgcggag accaggacga
     3661 tggtcagcca cgtctggctg accatgcgca ccatgccttt cgtgcgctgg ctcatggctg
     3721 aggacagttt ccggtcgtgg tcgaggatgg cggcgctgat tctcttcgcg ctttcgttca
     3781 gttccgcttt tacagatttc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     3841 tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa agactgttca
     3901 taaatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatactg
     3961 ctttttgtgg tgcggaccac ctcaaaacct gcgctttcca gcgcctcagt gacatcctga
     4021 cgggttttca gctccccgga tgaggcaaga gcaagcagac cgcgtgtaat cgcttctgca
     4081 gcctcctgct tcgctttagg cagtgcagac ggtgtgacca gtgcccgccg gttctccggt
     4141 gcgttcgggt cgtgcagccc cagtctgccg ttcacgatgg tctgccaggc atcgatgcgt
     4201 ggacggtcgg cgcgatcgta atacggctgg agccgtctgc cggtcagcag ctccgtgttc
     4261 gggatcagaa aattcagctc aagccgcccc ttgtcacgat gctccaccca caggatgctg
     4321 tactggtctt tatcaagtcc gggcatcaga acccgctcaa agcttgccat cagcttttcc
     4381 cgctggccgg agggtaaatc cgcttccgca aacgacagta ccccggaggt gtatttctta
     4441 acgtaaggcg acgcgtcaat cagctcccgg acttcttccg gcttcccctg caggacggtg
     4501 gcgccttcac gctgccggtc tttacccagc agatagtcca ccggcccggc gccaccgccg
     4561 cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg
     4621 cagccgctca agtccggcat cgatggccat cagcgccgca acaatc
//
