LOCUS       NZ_CP045314             4921 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain 14102KSN plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP045314
VERSION     NZ_CP045314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12999969
            Assembly: GCF_016864035.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Comparative multi-omics analysis of clinical Enterobacter spp.
            isolates uncovers differences in the expression of determinants for
            antibiotic resistance and virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Nepal,S., Gabarrini,G., Bonn,F., Grasso,S., Rosema,S., Couto,N.,
            Becher,D., Rossen,J.W.A. and van Dijl,J.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Medical Microbiology and Infection
            Prevention, University Medical Center Groningen, Hanzeplein 1,
            Groningen 9700RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. 11; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:33:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,457
            CDSs (with protein)               :: 4,457
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 64 of 94
            Pseudo Genes (internal stop)      :: 19 of 94
            Pseudo Genes (multiple problems)  :: 17 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="14102KSN"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
                     /geo_loc_name="Netherlands: Groningen"
                     /collection_date="2017"
     gene            complement(join(4879..4921,1..302))
                     /locus_tag="GC132_RS23140"
     CDS             complement(join(4879..4921,1..302))
                     /locus_tag="GC132_RS23140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_032623643.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPAR
                     TGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1288..2574)
                     /locus_tag="GC132_RS23145"
     CDS             complement(1288..2574)
                     /locus_tag="GC132_RS23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(3488..3703)
                     /locus_tag="GC132_RS23150"
     CDS             complement(3488..3703)
                     /locus_tag="GC132_RS23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3710..4201)
                     /locus_tag="GC132_RS23155"
     CDS             complement(3710..4201)
                     /locus_tag="GC132_RS23155"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(395..567)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          492..1038
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(4..281)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.548"
                     /coverage="87.0"
                     /evalue="5.91e-53"
     Typing          complement(3391..4889)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccgctcaa gcccggcatc gatggccatc agcgccgcca caatctgcac cctgtcatgg
       61 ccggtgccgc cgccggcgtt aacacggcgg gctatctggt tcaggttatt ccccatgccg
      121 gcaagctggc gcagcagcgc cggcgagatt gaggggagct ttccggtgcg cgcaggtttt
      181 tcgtccaggc aggtctgccg catccacgcc gccagctgcc tgccgtcaca gcgctcaagc
      241 agccgccggt gctcgtcctc cgtcacccac atggtgagca tcttgttccg tttctctgac
      301 aaaccggcct cctgataacg ccgggacggg cgggaaagcc tttcccggtc ggcgcggggt
      361 tggacaagcc gcaggcgcgt caggcttttg gcgggtttcg gggcgcagcc ctgaaccagt
      421 cacgtagcgc tagcggagtg tatactggct tactatattg gcacggatgc gaatatcagt
      481 gaagtgcttc atgtggcagg agaaaaatgc agcagcggag cgtcagcaga atatgtgata
      541 caggatatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      601 agcggtaccg gcttacaggc ggggcggaaa tttcctggga gatgccagaa agaagcttaa
      661 cagggaagcg ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
      721 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
      781 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
      841 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
      901 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
      961 cgccttatcc ggtaactatc aacttgagtc caatccggaa aaacacgaca aagcgccact
     1021 ggcagcagcc actggtaaca ggtttagagg aggagagtct tgaagtttcg ggtgcgataa
     1081 ggctacactg aaaggacagt tttggtgact gtgctcctcc aagccagtta ccgcggttca
     1141 aaagagcgtc aactcttgcg aaccttcgaa aaagccacct tgccgggtgg ttttttcgtt
     1201 ttcagagcaa gagattacga cgcaacggaa aggatctcaa gaagatcatc tgtgatgttc
     1261 gacagatatg gggatatgca ccatggccta tccggcttta actgaaatgg cgacatcctt
     1321 aaccgcagtc tcaacccctg cagcattcct ctgaatgctg gtcataactt cacccgcttc
     1381 gcgcatgagt gtaacccctt cccgagtgtt catcaggctg ttttcaatgc cacagagaac
     1441 atccttggcc agttgactat tgctggccac gacttgctca atttcttcag tcgcgctact
     1501 gacgctcgcc gcaagattac gcacttctgc ggcaacaacc gcaaaacttc ttccggatgc
     1561 gccagccctt gctgcttcaa tcgcggcatt cagtgcaata agtcttgtct gcatggcaaa
     1621 cttccggatg gtttccacca tatcgtcaat actttcagac tgattgttga gccgtgagat
     1681 atcggtggag acagccccca tcccctgcgc aattctgtca atcataagga tgctattttc
     1741 aataacattg gcaccgttct gcgcactttc tcgggtctgc acggccatat cccatgcatg
     1801 cacagcagcc tcttgctctc tctggtttcg caggacatca gccgtaacat ctgtagcgaa
     1861 tttcacaatt ttataaagct gcccatcact gttaaagaca gggttataag ttgcacggaa
     1921 ccacagaggg tctccctgac ggttaaggcg tggaaacaga ccagaaaaaa actcaccttg
     1981 attcagactc tcccagaaat gtcggtattc atcacttttg taaagcgtct cggcacagaa
     2041 cagacgatga tgcttaccta tgatttcatc ccgcttatag ccagtggcat tgacgaaatt
     2101 ttcatttgct tcaagcacga tcccttcagg attgaaggtg atgatggcca tagaacggtt
     2161 tatggcattt acaactgctt cctgctcaag agcagaatgc acgcgctcag aaatatcggc
     2221 agcaatttta atgactctga taacacggcc gcgcctgtca gaaacgggga tataactggc
     2281 ttcaagccat acggaacgat ttccctttgc cagccgcagg tacttgccac taaagctttc
     2341 tcccctggca aggcgctgcc agaactggct gtattccggt gaacttacca gcgagggcgg
     2401 gcagaatata cgatggtggt gaccgattat ttcatcagcc ctgtacccca tagtggagag
     2461 aaaaagcggg ctggctttat ttatcacccc ctccggactg aactcaatga caggcttatt
     2521 ctgacaaatt gcgtcatacg aaatggctga tcgctggagg ttcagggaga acatattttt
     2581 aagtgaaaac atatgaatca gattgcccgg ataatgtaag tactgccatg gttatcggct
     2641 actccttatg tagcgttata tattactgcg acagggcggg aaaaagcccg ctattcagcc
     2701 atgccggcat gatgtccggc tccggatttc acgcgtaaaa aacccgccat ctttagcggg
     2761 ctgaacaata agaaaatgtt catatttacg gcaaatccgc cggctccagc agagaagctg
     2821 gcgtctatca atcttggaag aaccagcgca gctccgcatg cattaacagg catcagaagg
     2881 caaaaaaatg gctcccatcc gggagccgga aaacactcaa ctgcctggat cgcagcacat
     2941 cctttgtgaa ggataattta agtatcggcc ggattgccgc gggcataatt ttttccgtat
     3001 ccgccagacc tcggctttta acataacacc ccttgcccgc accgcagaaa cggggttggc
     3061 tgcgtttccg gtgccagacg cgagaaagtg gcgacagaca tgacgaaaaa acaccaaaaa
     3121 cgcagccgct ggatattaca ctgatgcata gtcatgcagc cagatgcatg cagcgtaaag
     3181 tcacactaaa atcagcgcga acgccttaaa aaccggcaaa tgcctctccg gcaactcaca
     3241 cagctccatc accaggtaaa aacagttcag cagggcttca ataacgttga gaattttcat
     3301 cacttcctcc gggataagtc actaccattc cggtagtcag cctatttagc aggaacgcga
     3361 tgagcccttc ttcgtgcggg ctgtcacctc atcttcggac catcgaaact cttctgcttc
     3421 tgtcggagcg tcagctccag ctcgttctga cgctgatact ccagtgcagc cttctcctcg
     3481 atggcattca gttcccggac aagcggcgca gctgctcgct taaactcagc acctgcgtgc
     3541 tcaagtccga cacgcgctcg ttcagcaccg cgttctcccg ttgcataaga ccacacatgc
     3601 tccgccattc cgcgaaggcg ctctcccatt cgtccagcct ttgcgagtag tcctgctgta
     3661 gctgctctaa tacgctcagc aactgctttt ccagctctgt cattttccgc tactcctgtt
     3721 tcaggcggat ccatgtcgtc ccgtcgtagg ggatggcttc ggcttgcatc cccggcggca
     3781 ggataagaaa cctccggccg tcgctgctct cctgatagcg cactccccac gtccgggcgg
     3841 tcattttcgc cagggtatct tcctgctgac gaagatgcgt gtaattgtcg gttatctgct
     3901 gcccctgcca ccacagaatg ctcccgctcg tggcgatcag cagcgctgac accatcagga
     3961 tggtgagcca tgtcctcccg gccgtcagca acacgctccg ccggtttttc tccatcgcct
     4021 cgctcatact ctgctcgtgg gcgctgatgg cgtcgctgat tctcctcgcg cttgcgttca
     4081 gttccgctct gacagactgc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     4141 tcttctgctg cgctttcgat tgctgctcta agtccttcgc cagcgttaaa agactgctca
     4201 tagatggctc ccttcagtcg gatgtttcgc cccccgtcag ggtcggcaat gctgatactg
     4261 cttttcgttg tacgcaccac ctcaaaacct gcaccttcaa gagcctcagt gacgtcctga
     4321 cggcttttca gctccccgga cgaggctaag ctcagcagtc cacgggtgat ggcttctgcg
     4381 gcttcctgct tcgttttcgg cagtccggcc ggcgtgacca gcgcccggcg gttttccggc
     4441 gcgttcggat cgtgcagccc cagtctgccg ttcacgatgg tctgccaggc atcgatacgc
     4501 ggccggtcgg cgcggtcgta gtacggctgg agccgcctgc cggtcagcag ctcggtattg
     4561 gggatcagaa agttcagctc cagacgcccc ttgtcctcgt gctccaccca cagaatgctg
     4621 tactgatctt tatcaagtcc gggcatcaga acccgctcga agctggccat cagtttttcc
     4681 cgctggccgg gaggcagatc ggcctccgca aacgacagga cgccggaggt gtatttcttc
     4741 acgtaaggcg acgcgtcaat cagctcccgg acttcttccg gcttcccctg caggacgctg
     4801 gcgccttcac gctgccggtc tttacccagc agatagtcca ccggcccggc gccaccgccg
     4861 cggccacgtg gatgaaattt aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg
     4921 c
//
