LOCUS       NZ_CP045285             4072 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain LAU-OXA plasmid pLAU-OXA8, complete
            sequence.
ACCESSION   NZ_CP045285
VERSION     NZ_CP045285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13021417
            Assembly: GCF_009363035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Genetic Characterization of an IncFII plasmid carrying blaOXA-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Natural Sciences, Lebanese American
            University, Main Street, Byblos 36, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009363035.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,059
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 79 of 261
            Pseudo Genes (incomplete)         :: 191 of 261
            Pseudo Genes (internal stop)      :: 47 of 261
            Pseudo Genes (multiple problems)  :: 50 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045285.1.
            Bacteria and source DNA available from Dr. Sima Tokajian.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LAU-OXA"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAU-OXA8"
                     /geo_loc_name="Lebanon"
                     /collection_date="2017-12"
     gene            complement(86..2233)
                     /gene="mobQ"
                     /locus_tag="GBF15_RS25390"
     CDS             complement(86..2233)
                     /gene="mobQ"
                     /locus_tag="GBF15_RS25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057077964.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREVPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2412..2666
                     /gene="mobC"
                     /locus_tag="GBF15_RS25395"
     CDS             2412..2666
                     /gene="mobC"
                     /locus_tag="GBF15_RS25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            3199..3900
                     /locus_tag="GBF15_RS25405"
     CDS             3199..3900
                     /locus_tag="GBF15_RS25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1876..2234)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2235..2411
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          3200..3900
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.715"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(90..2320)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.658"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcttgcatt catccctgtc gatgaacatc gaagaacatc aacgaacata acacgatgtt
       61 aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg
      121 tggtgtgtgt tcttgcatct tcctcgcgcc gccattcgtc ccaggtacga ttgaaaccca
      181 gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggta
      241 cctcacgggt tttcagatga aactccataa gttccggcgc aggtgatatg tggctgtctg
      301 catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagtgcac
      361 tgatgaccag ctggctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat
      421 taagccggac aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct
      481 ggcggttttc ccggtcgaca gaactcatga ccggcatcag cgtcggtggc agacgtccct
      541 gcataaaatc caccttcctt gccgctggtg atggcatggc tggctggcgg tcttctccaa
      601 gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatgt tgcggccatg
      661 tctggcaaac ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca cgttgcagtg
      721 gcggacgttg gccgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat
      781 caacactctc aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtatatt
      841 ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatgggag
      901 cctcgccata ccccgtaacg actgctctca gcacccctga atccataacg gcattgtcat
      961 ttttcctgag ctgctccgcg cgactacgtt cagtatcttc ctgttccttc cggcgcaatt
     1021 cctcttcacg tttaagcaac tgggctctga gagctgcaag gccggattcc ccccactgct
     1081 gccactgttt ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc accttccggt
     1141 aatgctgtcg taatttttcc atttgctggc tcagagactg gatttttccc tgctcactac
     1201 ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg
     1261 atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg ttcagctctt
     1321 cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga catggcatta ctggtgagtt
     1381 tccatgccga gttactgtcc ttcggcaacg cttcgataat gtcacgtagt gtgtacgaag
     1441 ttgcgttgtc aggaagcccg atattcagaa ggtcagccac acggcgggca ggctctgtca
     1501 gccctccgtt cctgagttcg gtgcgggaaa tctgtatttc caggttaagc ctgttaatca
     1561 gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca caggcagact
     1621 ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta
     1681 tctcttcctg ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc catgatgccc
     1741 gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac ctttccgctg aacccagcag
     1801 gtccgacagc cctcgtggtc agcatgatgt gcgcatgggg atttgtttta tccatgtgat
     1861 ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca aaggcgagaa
     1921 cggtttcccg tgcggcatcc tgcggcagtt cgtgggggat ggcgagttca atttcccgcg
     1981 caagttgtga gttcttccgg cgttcggcct tctcaacggc attccagagt tcggcacggt
     2041 tcgtacacca ggcaggggcg gtggcggggg tcagaatcac agcctcagcc acaccgcttt
     2101 tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag catcagtgaa ccggaacgat
     2161 aggcagcgga agctacggcg ctgtagccgc tgcttctgga gataattttc atgctgagat
     2221 gataaatcgc catttctgcc gtaatgcctt tttgtagttt tctttcagcc ccgcagggcg
     2281 cacacaatcg aaacggagtg gagattgtat aagtgcgcac ttcgtgattt gccgtgcatt
     2341 ataccagctc agtttttaaa ctaccagtga cgcgctaaaa tgcgattttt catcgcgcac
     2401 ggagttacat catgagcaga acgctcgaac agaagattgc tgatgctgag gcgagattac
     2461 agcgcctgaa ggcgaagagc cggagcctgg acacagcgca gaaggttgtt gtgggtgctg
     2521 cattgctggc aaaggtcaga aagccggagg aggtgcagtt gcgtgcctgg ttactccagt
     2581 tcctgaaggc agaggtgaca cgacaggctg atgtgacgcg catactgccg ctgattaacg
     2641 agctggaggc gctgccagaa cagtgattgt atcgtccgct cgccgcagcc ccagcgaccg
     2701 agcgttagcg agcgagtggg cgaggaagcg gaagtgaatc agtcgccagg ttgagcacat
     2761 acgcacggtt tttcggcagg tgatatgcgc tgaacccgca tgaatactgg gtttccacag
     2821 ggttggaggg ggtgtattcg aaacttgcaa ttctgcttta ttgcaacgca ttgtttttat
     2881 tggttttttg tttttagcac cccaagttat ccacaggctc gccgttaagt ttttaagatc
     2941 ttatttttag atcttttttt taaaaccttt ttaggtcttg taacaccatg attttaaaga
     3001 tgttctggca agataagtgg agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag
     3061 gtggagcttt tgggggtgaa aggtggagct tttgggggtg taatccacag aaaaaggggg
     3121 ttgcaaaaaa aaaacttcca cccatatgat aagtggcgta cagtaaaaaa acgccaccaa
     3181 tggcaacgag caagcgaaat gtcagatctg atagcttaca aatcaaacgc ccttatcgag
     3241 gcaagctaca agctcacttt gcaggaacag cgcttcctgc tggtctgtat cagccgcctg
     3301 aaatccggag aagattcccc ttcgcctgat gaacaaaaaa ccatgaccat cacggctgca
     3361 gagtttcatg atgcctttcc tgacatggga cggcagcacg ctgaagctag gctcaaggaa
     3421 gcgatagatc gcctctggga caggtcagta atcatcaaga acgaggaaaa acgagaagag
     3481 ttccgctggg tgcagtaccg ggcgcagtat ttcaaaggtg aggcgaaagt agatatcacc
     3541 ttctccgatg ccatcatgcc gtatctgaca cagctcaagg ggcagtttac gcgggtagtc
     3601 gtgaagaacg tctccagcct ttccagcacc tacagcatcc gcatttacga gctgctccag
     3661 cagttccgca gcaccggtga cagaacgata gctatcgatg atttccgaac catgcttgcc
     3721 gttgaagata aatatcaaca attcaaagac ttgaataaga tgataatcaa gccagccatc
     3781 gccgaactga acgaaaaaag cgatctggtt gtaactgttg agacgatcaa aaaggggcgc
     3841 acggttgtag ccctgcattt tcgcttcaag gaagacaaac agatcaagat gacgatctaa
     3901 aatcgatttt aaggcaccta aaacgctccc tgctgcgttt tttttgtgtt agtagtgcga
     3961 aggtactatt gaagtgaaaa attgcgccaa aaacgctctg atgcagcgaa aaaagaaccc
     4021 ccaatttttg ggggcggaaa aacaaaacta tgcttgcgtt atgtgagtgt gc
//
