LOCUS       NZ_CP045284             5168 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain LAU-OXA plasmid pLAU-OXA7, complete
            sequence.
ACCESSION   NZ_CP045284
VERSION     NZ_CP045284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13021417
            Assembly: GCF_009363035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Genetic Characterization of an IncFII plasmid carrying blaOXA-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Natural Sciences, Lebanese American
            University, Main Street, Byblos 36, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009363035.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,059
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 79 of 261
            Pseudo Genes (incomplete)         :: 191 of 261
            Pseudo Genes (internal stop)      :: 47 of 261
            Pseudo Genes (multiple problems)  :: 50 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045284.1.
            Bacteria and source DNA available from Dr. Sima Tokajian.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LAU-OXA"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAU-OXA7"
                     /geo_loc_name="Lebanon"
                     /collection_date="2017-12"
     gene            1..927
                     /locus_tag="GBF15_RS25360"
     CDS             1..927
                     /locus_tag="GBF15_RS25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_021546191.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1122..1520)
                     /locus_tag="GBF15_RS25365"
     CDS             complement(1122..1520)
                     /locus_tag="GBF15_RS25365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_131199882.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELYYKKGMILQKLKSIKYDLDTYPNPDA"
                     /name="hypothetical protein"
     gene            1697..2623
                     /locus_tag="GBF15_RS25370"
     CDS             1697..2623
                     /locus_tag="GBF15_RS25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_021554642.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPDRGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2626..3159
                     /locus_tag="GBF15_RS25375"
     CDS             2626..3159
                     /locus_tag="GBF15_RS25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3213..3434)
                     /locus_tag="GBF15_RS25380"
     CDS             complement(3213..3434)
                     /locus_tag="GBF15_RS25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021554643.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRRLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     Typing          2..927
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.93"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1698..2620
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt tcggccttcg tatctccggc aaagggcgcg cgcttcttgc acggtatatc
      121 cagcagaacc agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcttcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag
      601 tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt
      661 tcactggctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgtggtcgca agggcgggaa aattggaggt gctaagtcta agcgtggtgc tgttgccaca
      841 tctgcgcgaa cgttgaaacc gtgggagact cttggaatta gccgcgcctg gtattaccaa
      901 ctgaaaaaac gaggtcttgt agagtagacc aaataaaacc tatatcagat aacagcgcct
      961 tttttgcgtc cttttgagtc gtaggttttt cgtcgtttat cccagcaatg gcgtttcttt
     1021 ccgcatgatt gcgctggtag tgctcgccgc agtctcatga ccgagcgtag cgagcgaatg
     1081 agcgaggaag caccagggaa ccagaatgtg aacgggaata cttacgcgtc tgggtttggg
     1141 tatgtgtcca gatcgtattt tatcgattta agtttctgta atatcatgcc ttttttatag
     1201 tatagttcgc ctgtttttgt gtctaccagt cctgcttcga tcagtagccc agccgcgaga
     1261 aagagccggt gattgcgttc ccgtcgttgt ttttttccct cgattcgttt ttggaccgcc
     1321 tgttttcgtt cgctctcctg tgcctgtaag tcgttttttt ccgctgttgc cagtacgtgg
     1381 agaagtttgc gttcctgttc tgtgagtccg gcttcgttgt cgatcaggtc aataatctgt
     1441 ctctgtcgtg cggtctgttc tccgagcttc ctgatgaatc cgatccgttt cttcagccat
     1501 tcgatgttgc tgatgcgcat gttcgtgatg ctccctggtg tttttttgcg gtgtgttgtg
     1561 gtcgtgcagt atatcgcttg cggtcggcgg gtgcaggggg tatcttccgt gctaattcgt
     1621 aaaattagaa ggaagcgcac ttatatgttt ctctccttcg tcgagaaaca gtgcgctcct
     1681 gcagagggct gaaaccgtgg caacctatca cctcagcgtt aaatttggtg gcaaggggca
     1741 ggctgcaaac cacgccgatt acatcgagcg caaagagaaa taccgcgacc gtcaggattt
     1801 ggaatattcc gcacacggaa acatgccgga atgggccagg gataatcctt cccatttctg
     1861 gcaggcggct gaccagtttg aacgtgcgaa cggttcgacc tatagggaac ttgaaattgc
     1921 attaccccgt gaactgacac cggaacagcg ccttgaactg gtgcaggatt ttgtacgtca
     1981 ggaagctggc gaacggcacg cctggagttt tgccattcac aatccgaaag cgagcattga
     2041 cggcggcgaa cagcctcatg cgcatatcat gatgagtcag cgtgtgaatg atggcattga
     2101 ccgtacaccg gaacagtatt tccggcgcta taacgccagg tatcccgacc ggggaggtgc
     2161 gaaaaaggat tccggaagcc tgactcccac tcagcaaaaa gagcagttgc gggaattgcg
     2221 aaaacgctgg gaggtgaagc acaacgaaca tatgagaaag catggctttg aaaggggctt
     2281 cattgactgc cggacgctga aagaacaggg cattgaacga cgtcccgaag tgcatctggg
     2341 ttttgaagct gccggacggc ttgatgatgc ccagcgtcat gacatcatgc aaaaacggtc
     2401 tgaaccggac tattaccgcc atcttgaaca ggcgcgaatg gtcgggcatg acatcaggga
     2461 atggctggat acaccggtta agcaacatga actggtcaga aggctgcaac atgatgttgc
     2521 gcgggagcat ggctttgaca tgagagacag agtgagcaga cagaaccgaa ctgaacctga
     2581 acgggttaaa tcaccgtcac gtggattttc tatgggattc tgaacatggc cagcacagaa
     2641 gaaaaattac tcacgctcct gttatcggtg gagcatctgc aacataccgc aatggagcaa
     2701 cagaaagctg ttgctgattc tctggctgtg gcaagggaaa ctcttcgcgg atatcgggat
     2761 atttctgacg gtgtggcaaa acaggtcagg gatgaagtca gaaatgaggt gcagaagctg
     2821 gacgtggcag gactgatagg tgagcgcatc agccaggatt ttcagcggct ggaagagtca
     2881 gtcgcaaaca tgaaggcaaa cgcggatgcc ctgaacaggg atattgcgac aattcacagc
     2941 aatatcgtgc aggaatacaa cacccttaaa gggtatagct ggaaatggct gggtggggtg
     3001 tcagtggctt ttctggttat gttctgtgtg ctcggatggg gaatgaaaac aatactcgac
     3061 aggaactaca acgccacagc ggcggtgtat cagaaagttg tgacactgga agataaactg
     3121 gtggagaaac ctgtcacggg taagagccgg aaaaactgac gcgcttcgtt tgtccaacgc
     3181 ctcacggagc gcgaaaggat ttcgcgaacc gttcaccgtt ttttccaata tgattctgtc
     3241 tgctttggtc gataaagccg tcgatagtat tccagccttc gtatatataa cggtacgtgc
     3301 tcatcagtaa gatctggcgg tattgtgtta tcagggggaa gttttttcag atactcacga
     3361 aatgcctggc ttgatgccat aaaatctaca gtagcatcga taatatgtgg ggtgttttca
     3421 cctaattttg ccatgatgat aatccttata ttatcacggt ttgtgaatga tagggtgtca
     3481 ctattattta tgcgttagtg aaccagaaaa aatcccccgt tggcataggg ggatatgtaa
     3541 ggagaaataa cttcgcaggt gttcttgttt gtataacgtg aaaagactgc acgcaactta
     3601 ataagtctgc cgtacttact ttcagttttc aacataaggt ttataacaat gtatggaatg
     3661 gaagtgagct ttaaggtttc agctctaaaa aataaaatat ttattagttg gctggttgtt
     3721 gttcttgttc tgtatgtata taaacactat cgacatatta tgaccgggaa cattccttac
     3781 cccctctgcg ctcgcagagg ttttttttgt cattaattaa taatgaactg aaatgcagtt
     3841 ccgatacctc catttgcaca ttatccctga catatggtac cggaaccgca ttaattttat
     3901 gttataagag tttattttta atgtccagat ggtttaatag tatttatatg taattagctg
     3961 tatggatagt actggtttta ttgtataact tgtcgttttt ttttattgtt tttgtgtggt
     4021 gtgctaatct tttagttata aaaaaacccc ttggaataca ggggcaaaag taaggaaaac
     4081 aaatggatgc tcagtttgtg tgacacttta aactaatgga actatagagt ggaggttgtt
     4141 acagtcgctt ttataaaaca gcaaccagtt tttttcaata gtctgatgat ggttgtgaca
     4201 gttttgtgat attgttcaaa atgtttcttg ttgaatgttt gttttttggt ctattggtgt
     4261 ctggttttat tttttctgct attacatatc aagaaatgtg attggtgtag caagagtgga
     4321 atgcgatgtg gataaactaa ttggttgtcg aatctaattt acttagaaaa ctacacttgt
     4381 ttttgtgtat gaatttagtg aatttgtctg tctatgtgtt ttcgtgcatt tataaatcct
     4441 tcgtcaggat cttaaattta gtgttgaata tgtagggcct gtacggtaat tattatttag
     4501 cgtagctact tcatcgttgt tcgaacacaa ccaacaacct gtaattgttg tttctcttgg
     4561 ttccccactt ttgtggggtt ttttatctct tccgtgcatc gtaaagcgcg gactccttct
     4621 ggaaaggttg ttttagggca tcactgaggc attttttgcg tttgtgaata ttgagcatcc
     4681 gataactaaa atatttccca tagactctga ttcttgccac cgaccccagg tcggtttttt
     4741 tttgtcctgg cgagatgtga agtttggaaa caggaaaccc cgtcagagcg ccacgcacca
     4801 ggggagaaaa cttgtcgttt tgagccgtcc ggtgttcaga acgcacgaaa ccgatcgcac
     4861 atcgctttcg tgcatagtta tgcaacgccc tgaaaacgat tctgacgcgt ttttctggtt
     4921 tggcctggtg ttttcttgtc tttttgcgtt ttttgcgcca gaaagcgact gagggcgttt
     4981 taaggggtac gtacaacggg agttatggta aatggatcgg atttgcggga aggctcgaca
     5041 ggttttgtcg ttgggggtag tgtaagcgac tgaaaaacaa acgcccctga aattcgtgtc
     5101 tatccgccaa gactttcacg aaaatacagg ggcgatgtcg gtcgataacc tggaaggaat
     5161 cgtaacac
//
