LOCUS       NZ_CP045283             7940 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain LAU-OXA plasmid pLAU-OXA6, complete
            sequence.
ACCESSION   NZ_CP045283
VERSION     NZ_CP045283.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13021417
            Assembly: GCF_009363035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Genetic Characterization of an IncFII plasmid carrying blaOXA-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Natural Sciences, Lebanese American
            University, Main Street, Byblos 36, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009363035.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,059
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 79 of 261
            Pseudo Genes (incomplete)         :: 191 of 261
            Pseudo Genes (internal stop)      :: 47 of 261
            Pseudo Genes (multiple problems)  :: 50 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045283.1.
            Bacteria and source DNA available from Dr. Sima Tokajian.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LAU-OXA"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAU-OXA6"
                     /geo_loc_name="Lebanon"
                     /collection_date="2017-12"
     gene            join(6771..7940,1..384)
                     /locus_tag="GBF15_RS26015"
     CDS             join(6771..7940,1..384)
                     /locus_tag="GBF15_RS26015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_052983339.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGDPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNL
                     PETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVMRSSISIADPNGGKN
                     IRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRY
                     SPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGPFPVYREWRDALVSW
                     RDDTGEPGRNQDTGRNIAETEREDMGRGVCAGREKEISGYPAGEIGNGDSLSGEGLGTT
                     DGVTQSDGAGKTFAERLRATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAV
                     ESAGAALERADRTLEPVIQREQEIRDERLIQEREHELSLERERQLEIQERTQDGPSLGW
                     "
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            987..1226
                     /locus_tag="GBF15_RS25305"
     CDS             987..1226
                     /locus_tag="GBF15_RS25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            1223..1585
                     /locus_tag="GBF15_RS25310"
     CDS             1223..1585
                     /locus_tag="GBF15_RS25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(1610..2503)
                     /locus_tag="GBF15_RS25315"
     CDS             complement(1610..2503)
                     /locus_tag="GBF15_RS25315"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(2506..4098)
                     /locus_tag="GBF15_RS25320"
     CDS             complement(2506..4098)
                     /locus_tag="GBF15_RS25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(4137..4835)
                     /locus_tag="GBF15_RS25325"
     CDS             complement(4137..4835)
                     /locus_tag="GBF15_RS25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(5383..5541)
                     /locus_tag="GBF15_RS26265"
     CDS             complement(5383..5541)
                     /locus_tag="GBF15_RS26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            5872..6059
                     /locus_tag="GBF15_RS26270"
                     /pseudo=""
     CDS             5872..6059
                     /locus_tag="GBF15_RS26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(6202..6393)
                     /locus_tag="GBF15_RS25345"
     CDS             complement(6202..6393)
                     /locus_tag="GBF15_RS25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6443..6781
                     /locus_tag="GBF15_RS25350"
     CDS             6443..6781
                     /locus_tag="GBF15_RS25350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015056440.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVR
                     EQGARDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          6039..6126
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(5470..6019)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.307"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..381
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.402"
                     /coverage="100.0"
                     /evalue="1.81e-39"
     Typing          6426..6775
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          6772..7922
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.229"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ctatcagggg aaggactggg tacaactgac ggagtgacac agagtgacgg agctggaaag
       61 acatttgctg aacgccttag agcaactgca acaggactat atgcagcggc tgaacgaatg
      121 ggagagcgcc ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat
      181 gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga
      241 acgcttgagc cagttataca gcgagaacag gagataaggg atgaacggct gatacaggaa
      301 cgcgaacatg agttatccct ggagcgtgag cgccagctgg aaatacagga acggacacag
      361 gatggccctt cgctgggatg gtaatcagag acaggctaaa atgttgtcag cgcgtttccg
      421 gtgcattttt tgggataatg gttggttcgg ggcaccggga acgcgcttca gtcagacctt
      481 gctgaaaaca caggaacgcg ttaaaacgca tacgggcgcg attctggtgt gtcttttgtt
      541 gctgtgtttt gcctctattg ccagatcgcg ctcattcccc tgaacttctg ttttcccgct
      601 ccggtccccg tcttcttcgc ttccgaaagc atgaaaaaaa tccgtttcag ctgtctctgg
      661 ctggcaattc ctgcgtgatt cacatggctg catagctatg catgtggtta aaatttaccg
      721 ggtgcgatcg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg
      781 atcgcgccga acgcgtttca gcggtgcgta caataaggga ttatggtaaa ttatcttgtt
      841 ccttagagta tgattcgagg tggtatcctg cacttattta aatatgcttg cttgggaaat
      901 tccaggttgc agtatggttg agttgtaatt ttcctatgat aggaaaaatg ttatattatt
      961 aatttatttg tgaggcttca taaagaatga aaatttctga tattgaaaaa aacatatata
     1021 aaggactagc tgaatttatt tcacaaaaaa tcagagatga acatgatata tgccttggac
     1081 agtttgaatc agaggaatta cttgatgaga ttcttaaaag agtaatccca gtcatatata
     1141 atgtggctat tgatgatgct ataaaatcag taaggaatgt tggggataga ttagaggagg
     1201 aattagatat gaggaaggta gtgtgactaa ttttaaatca gacttaaatc taataagaaa
     1261 agaatggtat aatgcatttt attcaggtaa tactgaaatg ctagattact tggaagctga
     1321 ttggtttatt tcaacaaatg gtcaaaaagt tatctataaa aaacatcagt tacgaaagat
     1381 tgatatgcgt ccagcgagga ttttacctag catagaaaga tatgaatatg atgtcgtaat
     1441 gcgtgagttt aaaggcattg cttgtgtttc agggaaagct gaaataaagc aaaatgatgg
     1501 aaatatatta atggcattta ttgagaattg gataaaggtt aatggtggat ggaaaatgca
     1561 gtttatatcg tatgaaaaaa tctaagggga agccccctta gattaatggt tatatatgtt
     1621 caagttttat ttttttatag cgataagttc tgctttgagc tttaagcatg ttttctgcaa
     1681 actgaatatc aaaactattt atagcattag ttattatagt ttttattttt tctttatcag
     1741 ataatgaatc accatgctct ataggttccc gcatatatgg attgtttttt aattcatcaa
     1801 ataatgtttt aaatagttct aatcgacgag gtcttataac tgtaaaagat atatgaagag
     1861 agtgtttgct ctgagtcgta acattgtgcc agtatccagc aggaagatat atggcctgac
     1921 ctggggttat atcaaactca taatctgggg aggtattagg tggtagataa tcgaagcttc
     1981 tttcatcttc gagagggtat gaataggtag ggttgtaaac tttccatttt tttattccct
     2041 caatttgaac agctattaca tcatgattgt caaaatgaag cccaaagctt ggtgttgctg
     2101 taaatgcagc atatagatta gcactacatg tacattctaa ctctttagat aaccatagtc
     2161 tgctttcatc tacagattca aagaatgact ggcatcgatc tactataagg gttgcgccat
     2221 cacgtaagca tttatatagc tgatggcgat ttatatgtgg tgttctatcg cctgtttggc
     2281 tatatgtgta tcttataaat cctttatacc ccctaatttc gggaaagttg tcatttgcca
     2341 ttctaactct aggatagtgc aatatatctt tttcaagcaa tttattaaga tcattccagt
     2401 tgattaagtt tttataatta acatccgtga atacaaacgg atatttctgg aaattatatt
     2461 tcttaaattt ttctatgatt tcctttttta taagattatt catttttaaa cctcatgttt
     2521 tgtgatatct ataatctgtg ctttaggtat attatttata agttgttcat catgagatat
     2581 aacaatgata tgcttttcct tgttttcctc ttttatataa tcactgatta tttttatacc
     2641 aagtgaatca aacccttttg tcggttcatc gaaaataatc aagtctacgt tttttccaga
     2701 tgtaagagat actcctaatt tttgtttttg tccaccagat aaattatgtc cgtaaccttt
     2761 gatctcataa tctagtggtt tgtcgaactt aaatctgtgt agcagctttt taattgtatt
     2821 taaattatta atgccggaat aatgtaatat attttcctct atagttccag gcatgagatc
     2881 tagatcttga ggaacgtata agacattaac aaaaggagaa tttgcttttt cttgttgtat
     2941 accattgatt tttattgtgt tttcttctgt tgttatattg ccagagagaa tttttgcaat
     3001 agttgttttt cccgaaccat tctcaccggt tataaaggta acaacgcctt ttgttaatat
     3061 taatttatgg ttgaatgatt tgttgtatat ttcaatattg caaatattgt cttttacatg
     3121 aagatattgc ttgtctgtgt aatttgtttt ttttatgtcg tttatttcgg cacagtataa
     3181 atcaatttct gttcttgcca tttttatttg tctaaataga taaccaatgc gatttaatgg
     3241 gagaaaaact tggaataaaa aagtggcaag cataataata ctaccacggc ttgctctacc
     3301 atgaagaaac atccagtatg ctgataggat aatcagacta ctaaaaatga ctgaaagaaa
     3361 agcggagctg ataccaaaca ggaaaaattc attatttact tttctttgtt tttctgaatg
     3421 tgtatttaca gcttgaatta ctcgtttacg acttttatcc gtggtatgga aagctttatc
     3481 cataggatac atgtgcatca tatcaaacat tcttccagat gcgaatttgg agctaagtgc
     3541 aactgatttc atcattggaa atctacgttg tgtcattttt atgacaaata ttatatagat
     3601 tgccattatt attataaact caatggctat taatgtatta acacttatac aaattgttat
     3661 gattattcca atcatttgta ttaataatgg tactacatca gaacatattg tataaaataa
     3721 agatgatgca gaagaaagca tgctttctat aaccttattt agttctcctt tatttttctt
     3781 taaaaaatgc tcttggtgag aattttgtat attatcaata acttgctctc taagctttac
     3841 agataattct atatttgttt tttgagtcaa gtattccgat atgctatatc cagcaggcat
     3901 aaggaatcta attgataaga aacatgctat aaatattaat aatgagttta tttctcctat
     3961 tgaatcttta ttgtcaataa ctgtttttat taaatatccg aatagaacga aaagaaaact
     4021 acttatagtt gataataata taataaaaaa caaattacat ttattaaatg tgatgatgta
     4081 cttttcaata aaattcaatt tataatcctt aaagcccgta tattacgggc tttatgttat
     4141 ctagaattac atgagcaccc agcgccacat cctttaccgc tgcaaccaca aacagaacca
     4201 ttatcattcc aaacatcttt tcgcattatt atttttacaa attctgcggc tggaaggtga
     4261 gtgatactct ttactttttc gatgtaagaa tcactggata tggattcttt tggaattctt
     4321 atagtattaa tgttctttat gctgtatctt acagcataat caccatacca ctttgcgaca
     4381 gtttcatgac ctaggtaaaa agcgattttt tcaatttgat ttttatattc gttaaaacga
     4441 tattcttgga atgttatacc atgctcatta acatgcctta tctcattttt tagcatgtca
     4501 tttgtatttg gataatggtg gaggtaagca ccaagatatt tttcacaaaa gctggtataa
     4561 gcatctgtaa attgaataaa cgtatgccac atttcatcta taatttgtaa ttgctcgtga
     4621 acttttatat tgtagcattc cgttaggcat gttgaagcta gccagatgaa tttctttgtt
     4681 tcattaaaga tatcatcagc ttcttctggt gttacattcc attctttacg aaattttaga
     4741 agaacatcat cattgtgata actcatgata tcttctaatg agctaacatt atgaactttt
     4801 gcataagaac tgctgttcat cttatgttgt agcataacac ccccaattat atatttgaat
     4861 gtagatgaga atagttatca tttgttatag tattgttaaa tttgtgcttg tgtcaatatg
     4921 cctaattcta taaatgagat agtaatcaag ttttttaatg gttataggat ccattagtaa
     4981 tacaaggttt ttgcagccag tttttacggg taggggctgc aaggaaaaca aaattttccc
     5041 actctaagag aagagaattg agttggttga atgagtggat gcatctaggg ccgctaattc
     5101 atcgagtgta aaggctgtac gaaatgtgtt gcagtccgat aatagccccc tccattgacg
     5161 ttttacacat gtgttgtagg ttccaggagt gggcggatct caaaaagatc aaaggatctt
     5221 cttgagatcc tttttttctg cgcgtaatct gctgccagta aacgaaaaaa ccgcctgggg
     5281 aggcggttta gtcgaaggtt aagtcagttg gggactgctt aaccgggtaa ctggcttggt
     5341 ggagcacaga taccaaatac tgtccttcta gtgtagccgc agttaggcca ccacttcaag
     5401 aactcttaat atctcaatcc accttgtcca gttaccagtg gctgctgcca gtggcgcttt
     5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgagccgag
     5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5761 atgatgctcg tcaggggggg cggagcctat ggaaaaacgc caacggcgcg gccttttgtt
     5821 ggccattgcc tttttgctca catgttcttt ccggccttat cccctgattc tgtggataac
     5881 cgtattacgc ctttgagtgc gctgataccg ctcgccgcag ccgaacgacc gagcgtagcg
     5941 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggca ttttctcctt acgcatctgt
     6001 gcggtatttc acaccgcata tggtgctctt ttcttataat ctgcactgat gccgcatagt
     6061 taagccagta tacactccgc tatccgctac gtgactggtt cagggcttcg ccccgaaacc
     6121 ccctgacgcg cccttacggg cttgtctgct cccggcatcc gctcacagac aagctgtgac
     6181 cgtctccggg agctgtatgt gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca
     6241 gctgcggtaa agctcgtcgg cgtggtcgtg aagtgattca caaatatcgg cctgttcatc
     6301 tgcgtccagt tcgttgagtt tctccagcag cgttaatgtc tggcttctga taaaacgggc
     6361 catgttaagg gcggtttttt cctgtttagt cactgatgcc tccgtgtaag ggggatttct
     6421 gttttatggg gtaatgatac cgatgaaacg cgagaggatg ctcaccatac gggttactga
     6481 tgatgaacat gcccggttac tggaacgctg tgagggtaaa cagctggcgg tatggatgcg
     6541 gcgggtctgc ctgggtgagc cggttgcacg ctccgggaag ctgccgacac tggcaccgcc
     6601 gttactgcgt cagctggccg ccatcgggaa taacctgaat cagacagcga ggaaggtgaa
     6661 cagcgggcag tggtcttccg gtgatcgggt tcaggtgttg gctgcactga tggccatcgg
     6721 ggaggagctg cgtcggctgc gtctggccgt cagggaacag ggcgcgcggg atgattgtta
     6781 aatttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg ctgggaaggg
     6841 agcgaaaccg ggaaggggca acggtgcttc agggtgatcc ggaagaagtt cgggaactca
     6901 tcgatgccac gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc gcggagaagg
     6961 agctgccgcc gggaggacgt gaaaaggtga tggcgagctt tgagcgtgta ctgatgcccg
     7021 gtctcgaaaa gaatcagtac agcatcctgt gggtggagca ccaggacaag ggacggcttg
     7081 agctgaattt tgtcatcccg aacatggagc tacagaccgg aaaacgcctc cagccgtact
     7141 acgaccgggc agacaggccg agaattgatg cctggcagac gctggttaat caccattacg
     7201 ggctgcatga ccccaacgcc ccggagaacc gccggatact gactctccct gataacctgc
     7261 cggaaacgaa acaggcgctt gcagagagcg tcacacgcgg gatagacgct ctttatcatg
     7321 tcggggagat aaaaggccgt caggatgtga ttcaggcgct cacagaggca gggcttgaag
     7381 tggtcagggt gatgcgaagc agtatcagca ttgcagaccc gaacggcggg aagaatatca
     7441 ggctgaaagg agcattttat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag
     7501 ctgaaagaga gagccgaatc tacagagaca atgctgaacg acgagttcag caagctcgaa
     7561 aaatctgtaa gcaaggctgt gacatcaaac gagacgaaaa tcaaagacgc tatagccctg
     7621 ttcacagctt cgacagagga atcactgaaa aaacaccggg aaggggtgaa agaggcgatg
     7681 atgcagcaca ggaaggacgt gttaaagctg gcagggaata cgggcatgat gttactgggg
     7741 atggtccttt tcctgtttac cgtgagtggc gggacgctct ggtatcttgg cgggatgata
     7801 caggcgaacc tggaagaaat caggatacag gaagaaacat tgcagaaact gaacgcgaag
     7861 acatggggcg tggagtttgt gcaggacggg agaaggaaat ttctggttat cccgcagggg
     7921 aaatcggcaa cggtgattcc
//
