LOCUS       NZ_CP045282            27029 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain LAU-OXA plasmid pLAU-OXA48, complete
            sequence.
ACCESSION   NZ_CP045282
VERSION     NZ_CP045282.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13021417
            Assembly: GCF_009363035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27029)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Genetic Characterization of an IncFII plasmid carrying blaOXA-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27029)
  AUTHORS   Moussa,J., Panossian,B., Nassour,E., Salloum,T., Abboud,E. and
            Tokajian,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Natural Sciences, Lebanese American
            University, Main Street, Byblos 36, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009363035.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 04:00:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,059
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,674
            CDSs (with protein)               :: 4,674
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 79 of 261
            Pseudo Genes (incomplete)         :: 191 of 261
            Pseudo Genes (internal stop)      :: 47 of 261
            Pseudo Genes (multiple problems)  :: 50 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045282.1.
            Bacteria and source DNA available from Dr. Sima Tokajian.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LAU-OXA"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pLAU-OXA48"
                     /geo_loc_name="Lebanon"
                     /collection_date="2017-12"
     gene            1..858
                     /gene="repA"
                     /locus_tag="GBF15_RS25065"
     CDS             1..858
                     /gene="repA"
                     /locus_tag="GBF15_RS25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_000130646.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDVSEE
                     AVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            1797..2450
                     /locus_tag="GBF15_RS25075"
     CDS             1797..2450
                     /locus_tag="GBF15_RS25075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000616807.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLFF
                     SHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALALPA
                     EPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            2543..2800
                     /gene="pemI"
                     /locus_tag="GBF15_RS25080"
     CDS             2543..2800
                     /gene="pemI"
                     /locus_tag="GBF15_RS25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_000557619.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
     gene            2802..3134
                     /gene="pemK-mt"
                     /locus_tag="GBF15_RS25085"
     CDS             2802..3134
                     /gene="pemK-mt"
                     /locus_tag="GBF15_RS25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="WP_000439434.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPV
                     TSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGR
                     LSTILT"
                     /name="pemK-mt"
     gene            3383..4080
                     /locus_tag="GBF15_RS25090"
     CDS             join(3383..3632,3632..4080)
                     /locus_tag="GBF15_RS25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(4074..4397)
                     /locus_tag="GBF15_RS25095"
     CDS             complement(4074..4397)
                     /locus_tag="GBF15_RS25095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="WP_019725063.1"
                     /translation="MNTDNYFFRVPATRGIQGGIEQYMLTVPMVVLRRILAMDNDGDVM
                     DRSQREANKTRAKKIRNYVAGATSKRAPYILPSITGNIDSHVEFLPSELSPAVGILVMT
                     PTY"
                     /name="DGQHR domain-containing protein"
     gene            complement(4461..4727)
                     /locus_tag="GBF15_RS25100"
     CDS             complement(4461..4727)
                     /locus_tag="GBF15_RS25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805667.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_004187367.1"
                     /translation="MGNKYTVPLNVGAIRESCFRPADKWTPPTGDELRYLLENVLNLSQ
                     EGLARHVGVNGRTVRRWVNGESDIAYSVWCVLCIDAGLPPIWK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4772..5221)
                     /locus_tag="GBF15_RS25105"
     CDS             complement(4772..5221)
                     /locus_tag="GBF15_RS25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="WP_004187369.1"
                     /translation="MKFSAEELNIIRQADAIIAGKITTTDFITGADAARSLLRFRLAAE
                     PRESFCAFFLNCHNGVIAFEELFKGTISSVTVHPRIVLQRALHHNAAAVVLAHNHPSAN
                     TTPSQADKLITERLAQVLGMVDVKVLDHLIVGGSDIYSFAEHGLI"
                     /name="JAB domain-containing protein"
     gene            complement(5343..5705)
                     /locus_tag="GBF15_RS25110"
     CDS             complement(5343..5705)
                     /locus_tag="GBF15_RS25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3085 domain-containing protein"
                     /protein_id="WP_004187371.1"
                     /translation="MTMQSELVFTDPMLNVVIAEVKRFNCPLLFVKDHGVYVMAAKGEK
                     NSNGMHNVCYANGFNPDTTDFDELWDRMRDACGGDDFCESLELDPRSIELLSRTKPCLK
                     IMLSETELEVIAGGQK"
                     /name="DUF3085 domain-containing protein"
     gene            complement(5727..5990)
                     /locus_tag="GBF15_RS25115"
     CDS             complement(5727..5990)
                     /locus_tag="GBF15_RS25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187372.1"
                     /translation="MTALNSQQQADVARYIREHGNKTVISPEWSLIYFSLCQCELHQSH
                     EAIIFHNGRPAGLFILNETGELTELYLLGSLSDILTAAAVNK"
                     /name="hypothetical protein"
     gene            complement(6108..6518)
                     /locus_tag="GBF15_RS25120"
     CDS             complement(6108..6518)
                     /locus_tag="GBF15_RS25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004206907.1"
                     /translation="MITTRRMLMLYACLVPVALTGCDLADYFPNVPQPVPVISPGYQVD
                     IDGHPVRISGFDDCPREKDEARHAAAQDEKGCIVLSAERSEVKVRLYQPAGAVTETWRI
                     IREKDARQNDIIRLQRPNGALVMQNLQGKESP"
                     /name="hypothetical protein"
     gene            complement(6547..7026)
                     /locus_tag="GBF15_RS25125"
     CDS             complement(6547..7026)
                     /locus_tag="GBF15_RS25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187375.1"
                     /translation="MIKMVSVVPQPETVKTLREKMGMTETALGAVMGYELRAWQRKEAI
                     SDDLSQYNKTSLRPGEYNMLMLIAGVHPDYRLNRAFSPDDMVKDPATAEDVRRLRLALG
                     LKHAEIAALFGYKPASWQTKEKAAQRGVKLKTGEFNFLLLLAGEHPSLQLVEKAK"
                     /name="hypothetical protein"
     gene            complement(7019..7525)
                     /locus_tag="GBF15_RS26005"
     CDS             complement(7019..7525)
                     /locus_tag="GBF15_RS26005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187377.1"
                     /translation="MQSFPLFVSRVVPVITLSHCLRQRRWSLHNTPCQIDLRSGCHLRS
                     QPFGTLSRREISDRPPAMPRGYWFRQCSRDVMFTGRIDQADPMKQVYYNEGWSGPNKYT
                     FEVYQLENGSYRALARKWNGKINKVQQETQYLSDTREGLKHQDYPRTRQVKIFLNSDFW
                     EKGND"
                     /name="hypothetical protein"
     gene            complement(7643..8206)
                     /locus_tag="GBF15_RS25135"
     CDS             complement(7643..8206)
                     /locus_tag="GBF15_RS25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="WP_019725065.1"
                     /translation="MKKYVLLTLTLIAGAVAANPVTAAGNIAGDVQAQAATEVKTGFSP
                     GGTALNLVLDSIDHAQHEILVAAYSLTSRPVAGALRAAKKRGVSVVVVADRKANDNRYT
                     EVNFLANNRVAVRINDNYEAMHDKFMVFDRRAVLTGSFNFSASADSRNAENVVLISGAP
                     AVTEAYVNEFSRLWDEGKDVAPRY"
                     /name="phospholipase D family protein"
     gene            complement(8216..8659)
                     /locus_tag="GBF15_RS25140"
     CDS             complement(8216..8659)
                     /locus_tag="GBF15_RS25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_004187380.1"
                     /translation="MTDNRKCSFYIYPERNAADRVADGFLEKLPQKERGRAMRAMMLCG
                     AALMKQDERLPFLIAEFLTESTTMQDIQRIISSTLPQQDTGEMARLVEAFLQATGGGSN
                     TVSPAGGAAPENSPAVAPEPAAPEDKNLEETRKNVQHLFPDDD"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(8662..9636)
                     /locus_tag="GBF15_RS25145"
     CDS             complement(8662..9636)
                     /locus_tag="GBF15_RS25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="WP_004187382.1"
                     /translation="MRIFVDDGSTNIKLAWMEEGAVKTLISPNSFKPEWSMTLLTDSSV
                     PASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIEPQPVDVVVT
                     LPLGEYLDENDQPNMANIERKKANVKRAVAVQGRESFTVRKVSVLPESVPAGFSVLKDL
                     DDLDSLLIVDIGGTTLDIAHVRSKMSGFTKTHCDPKTGVSIITEAVKHALDTSVSTRTS
                     SYFADRLIQARNDETFLSRYLQNADQRAQVMKVLHEREKALTHRVTDSVGRFAGFTHVM
                     VVGGGASLVAGAVKQATGVSDDRFFVSDNPQFDLVLGMVAMKG"
                     /name="plasmid segregation protein ParM"
     gene            complement(9877..10617)
                     /locus_tag="GBF15_RS25150"
     CDS             complement(9877..10617)
                     /locus_tag="GBF15_RS25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187383.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_004187383.1"
                     /translation="MNTFPLQISQNSRSDAIGTGRVTYAYFDYADALALREMASQADLP
                     KYLLAPEVSVFLRYVPDLRQQVLFDTLWNTGARINEALALTGASFQLDGSRPFVRLKTL
                     KQRQRGRGRPGKDEEVFRLVPLTDPQYVRKVREFLTTLRIGKQQLLWPVQSDNTPRNWI
                     RKALDLAKRDSVTFSIPVTCHTFRHSFCMHLIQHGVPLKVVQAYAGHSRLETTETYTRV
                     FALDVGRQYGVRFSLPDSHLTLPE"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(10636..10884)
                     /locus_tag="GBF15_RS25155"
     CDS             complement(10636..10884)
                     /locus_tag="GBF15_RS25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187387.1"
                     /translation="MSGLKGRRISADMFAMMLKVSAGQLVFAARHDKPLRGLTLPPYRI
                     EGGKSWSSTASTRSMTFDMQDAVEFSEKFHKIQSPDG"
                     /name="hypothetical protein"
     gene            complement(10881..11366)
                     /locus_tag="GBF15_RS25160"
     CDS             complement(10881..11366)
                     /locus_tag="GBF15_RS25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187390.1"
                     /translation="MKFKGYVAALPALLLTGCAMLPGQPTDYDRFCNVSGIASHGETYR
                     VSDSQDFWLTPNGRYLSQAEYSSPADTLQKLTGVVSGEDPDQVRKNAVRVRVFRVESEN
                     SHKGACLPVRYDDNGAQRKMDSLTNGRRMVVFSEDEGQSGQQIYNKSRGTGFSYRLL"
                     /name="hypothetical protein"
     gene            complement(11363..11950)
                     /locus_tag="GBF15_RS25165"
     CDS             complement(11363..11950)
                     /locus_tag="GBF15_RS25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805651.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004519 - endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="WP_004187392.1"
                     /translation="MNESIHVVGSFILAGILLYGLWQGTRRRRRRHERKQASAVRVIDK
                     INTFPHFGQKIAYLRKIDPFVFEELLLEGFERRGFEVIRNRRYTGDGGIDGRVKIDGQT
                     WLIQAKRYTSYIAVGHVRDFSDLLNATGARGFFCHTGKTREGTKSLIRGDSRMILVSGQ
                     KLLDLIATDAPFIPYPGYRPPTEMVHPEQETT"
                     /name="restriction endonuclease"
     gene            complement(11943..12191)
                     /locus_tag="GBF15_RS25170"
     CDS             complement(11943..12191)
                     /locus_tag="GBF15_RS25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187394.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_004187394.1"
                     /translation="MTGFDLILWRRGLNWTQERAAAELGISRTSLVKYEDGETVPRTIQ
                     LATVALTLKAEWPTLKTMSKDRLLRQLKYEVLRIDNE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(12188..12649)
                     /locus_tag="GBF15_RS25175"
     CDS             complement(12188..12649)
                     /locus_tag="GBF15_RS25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187397.1"
                     /translation="MIKKPLIAGIMLVVAHTAGAAQVDDTNLQQIYTEAGIQGICSAGM
                     LAVPEGARDEVVPQNVRHLLVQCAYFDEQQLRRRAGLPAQTFSHWRDRNAVLNLSSDQV
                     SDALWLSAWRDFRAQLASAYPAPAEKKAQGKEAVPAGTKPGTEHGSAAD"
                     /name="hypothetical protein"
     gene            12790..13335
                     /locus_tag="GBF15_RS25180"
     CDS             12790..13335
                     /locus_tag="GBF15_RS25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187400.1"
                     /translation="MDINYYDKHQEEFEAVTLALKANLEEVWGSSLKNQGESLDDQVTY
                     MKLFEELQYNLNPYYFKENTSAKEMDEDKVAAFVARTRDYKHGITIKSWPGRPQKWLKG
                     RIKPLHPVEGTNLCWIDTSNIVHIGADRQFDDQYYLTVTTQNGQSYRVNDVLLPGRLLD
                     AAHEALFRALDSSTGGNF"
                     /name="hypothetical protein"
     gene            complement(13641..14225)
                     /locus_tag="GBF15_RS25185"
     CDS             complement(13641..14225)
                     /locus_tag="GBF15_RS25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059997.1"
                     /translation="MATKHINDELWHRIEVLTVRANARQNLIRPVKEADVLHLVLQRGL
                     ELLTDDDLLQLGKYRRPIGFVLRRPGMEMLKLDTLSMADAATILMRSGPATLCIWSRDD
                     ILRQASEAVIRERLPEMALLSEGDDRARFQTLLPGVWNAANRGETAVISLRADNADLAI
                     ARITDLMCESLLGYKGQRAYRAGENEQGEES"
                     /name="hypothetical protein"
     gene            complement(14225..14383)
                     /locus_tag="GBF15_RS25630"
     CDS             complement(14225..14383)
                     /locus_tag="GBF15_RS25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187405.1"
                     /translation="MMRQGVISAVIYLLLAMLVMIYMPEGYSAISWAIGAVVVVTLIYI
                     RARKGED"
                     /name="hypothetical protein"
     gene            complement(14408..14752)
                     /locus_tag="GBF15_RS25190"
     CDS             complement(14408..14752)
                     /locus_tag="GBF15_RS25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187406.1"
                     /translation="MEYEAYKVRGYGFGLKKEITGVLGRYPTQEEAQKVADDYTQACLP
                     DNLPDSYDMGMVRPVAAGEPAALDTDVFRGPGAPFMMDAGQLSTGQTVCLGKAADGKRF
                     YVGGEHSGGQ"
                     /name="hypothetical protein"
     gene            15279..16466
                     /locus_tag="GBF15_RS25195"
     CDS             15279..16466
                     /locus_tag="GBF15_RS25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_015059996.1"
                     /translation="MTGFFERKKRAKNAQLTVRLDEQLKKRAELRLEAMGMTATEAVEQ
                     LYRFIADHGRMPVTERIVTFDIQSQRGRAIALNPADAVTENTRRFLQSLGAVEVVCCDP
                     AAFEQAVLEYFSLYVADQAACLLAGVLNVTFMVNAASRVKDDWHDRLLSLLQRDFDFSE
                     EQSVTFINSIVDSEHVDPAIVSRNNTAWRSGKSTGSGAAVLQRFLTFLAERGVSCSDPQ
                     RLTESAGRAWGVRENMIGNMPPDMQQAINDGVIFEASADTPDFSPELAAAFGFTQAQAE
                     AVLKPALAARDLPGFLQRFLAFLADRGISCSNPRRFTEKVTEHASHEGLHRLIAMLMSR
                     IRDEGVCFVFHNESPDELPRLAASLPGELADTFGFTGWQATTILRQIQPARPGQD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            16485..16757
                     /locus_tag="GBF15_RS25200"
     CDS             16485..16757
                     /locus_tag="GBF15_RS25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059995.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_015059995.1"
                     /translation="MKTTCYADGITAAPLSPQEWRALLRAKGWKQKDLARRWNVSETWI
                     SKLANNPDRGTHWNDACRGLPDMRARRPGEPRAAGTDAPEPGGPE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            16754..17437
                     /locus_tag="GBF15_RS25205"
     CDS             16754..17437
                     /locus_tag="GBF15_RS25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059994.1"
                     /translation="MNKRLHLITTCTRRKAGSVEDTVFPGDTSLDDAFDGWYRLIAQAA
                     RRPGRVLETGELYRGAHWLRARGIAARWPGIELWAMSAGLGLRHHTDPAVAYEATFHAM
                     PFAPATVWGSLTARPPLAGRCASLAELMQRCPDDRFVIAGSPVYISAAEQDILAGVPAL
                     HDAAAQLIIVTSQGYRGPLQPFLKRSRADMMATLKSNMTCLNIACAGALIDAMMQADAR
                     QAATI"
                     /name="hypothetical protein"
     gene            17482..17700
                     /locus_tag="GBF15_RS25210"
     CDS             17482..17700
                     /locus_tag="GBF15_RS25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187411.1"
                     /translation="MFSIIYHAGAAVLFLVMSLAAGAGLLLHSHEYTTGHFWNMTGLCI
                     VSTLVWIWAVAQAKEAWYISRNIKKGL"
                     /name="hypothetical protein"
     gene            17703..17912
                     /locus_tag="GBF15_RS25215"
     CDS             17703..17912
                     /locus_tag="GBF15_RS25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187413.1"
                     /translation="MKDEIRHEKPVEVNIQLTHREAQALAQLVKRLGFSDCRGLATSDI
                     EAYLMMDGINQIMKALAEEGYAPR"
                     /name="hypothetical protein"
     gene            complement(18035..19300)
                     /gene="umuC"
                     /locus_tag="GBF15_RS25220"
     CDS             complement(18035..19300)
                     /gene="umuC"
                     /locus_tag="GBF15_RS25220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561122.1"
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="WP_019725070.1"
                     /translation="MYALIDVNSMYCSCEEAFRPDLRGRPVVVLSNNDGALVAVNRAAK
                     ALGVRRGEPYFRCRRLLEQHNVAVFSSNYTLYASFSARFATVVESLAPRTFVYSIDELF
                     ADASHMEGVMTPQAFGQLLRAEVLRQTTLTCGVGVAPTKTLAKLCNHAAKTWPATGGVV
                     ALSDPVRLKRLMSLVSAGDVWGVGHRTEKGLATMGIKTALALAEMDIRLARRLYGVTLE
                     RTIRELRGEACFALEEGTGAKQQLVVSRSFGSRVTQLAQMQQAVTKYATRAGEKLRQEN
                     RLAKVVTVFIRSSAYDAGGVPYSNQATEVLPVPSQDTRDIIAAAQLGLSRIWREGVRYA
                     KAGVMLSDLQGYETQLDLFSPAAVRPGSEKLMATIDKINREGRSRIFFAGEGIDPEYAM
                     RREMLSPAYTTQWKDIPAAFMR"
                     /name="umuC"
     gene            complement(19288..19722)
                     /gene="umuD"
                     /locus_tag="GBF15_RS25225"
     CDS             complement(19288..19722)
                     /gene="umuD"
                     /locus_tag="GBF15_RS25225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187425.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_004187425.1"
                     /translation="MALDLVYPRPSVEGDALPFFAEPVAAGFPSPAAGYEANELNLHDY
                     CVRRPAATYFLRVSGDSMRDARIHDGDVLVVDRAEEPRHGSIVIASIDNEFTVKQLQLR
                     PYPCLMPLNPAFPPIRFDPEALQIWGVVTFTVMRHRPCTP"
                     /name="umuD"
     gene            complement(19815..20147)
                     /gene="pemK-mt"
                     /locus_tag="GBF15_RS25230"
     CDS             complement(19815..20147)
                     /gene="pemK-mt"
                     /locus_tag="GBF15_RS25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="WP_004187427.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPV
                     TSGGNFARTAGFAVSLDGAGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMDDVLGR
                     LATILT"
                     /name="pemK-mt"
     gene            complement(20149..20406)
                     /gene="pemI"
                     /locus_tag="GBF15_RS25235"
     CDS             complement(20149..20406)
                     /gene="pemI"
                     /locus_tag="GBF15_RS25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_004187429.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PHRRPQYSLAELLAQCDPNAEISAEEREWLDAPAAGQEEI"
                     /name="pemI"
     gene            complement(20498..>20725)
                     /locus_tag="GBF15_RS25240"
                     /pseudo=""
     CDS             complement(20498..>20725)
                     /locus_tag="GBF15_RS25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            complement(20738..21946)
                     /locus_tag="GBF15_RS25245"
     CDS             complement(20738..21946)
                     /locus_tag="GBF15_RS25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS10A family transposase"
                     /protein_id="WP_001206290.1"
                     /translation="MREANILQHSLHQYCPELHLKRLNSLMLASKALIECKTLTLTELG
                     RNLPTTARTKHNIKRIDRLLGNTHLHQERLAVYQWHASLICSGNPMPIVLVDWSDIREH
                     KRIMALRASIAFNGRSITLYEKSYPLSEQCSKASHNGFLADLAKILPLHVTPLIVTDAG
                     FKVPWYKEVEAHGWFWLSRIRGTVQFADIGAENWRAVRSTHDLANGQAKSLGCKTLTKT
                     NPINCHLTLYRSKPKGRTNQRSTRTNCHHPSAKTYSTSAKEPWVLASNLPPESRSPKQL
                     VNLYAKRMQIEETFRDLKSPAYGFGLRQSRTNSPERFDIILLIALMVQCLLWLVGLHAQ
                     QQGWDKHFQANTIGHRTVLSTIRLGLEVLRRPDYQITEKELLAAWVLFANQLLKYGYAM
                     ADL"
                     /name="IS4-like element IS10A family transposase"
     gene            22249..23160
                     /locus_tag="GBF15_RS25250"
     CDS             22249..23160
                     /locus_tag="GBF15_RS25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586849.1"
                     /GO_function="GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_015586033.1"
                     /translation="MPDLNGMMLFAAVVRAKGFSQAARETGHPKSTISRKIAQLEEQLG
                     VRLLQRDTRNLSLTQVGALFYQHCDSIRNEVEAAKAVIESTHDDVSGSLRIAIPVSFSQ
                     ELIANLCSGFMRLYPNVELDVQFTDNDIGLVGEGYDIAIKYGPLQSSDLVARLLFERQP
                     ILVASPGYLKARGTPATPKELSDHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNS
                     PIMVKQLALDDFGIAMLSNSACKTELANGQLVPILQEWPIEPFKVYGVYSSRRQLATNI
                     SAFLDFFVKRFSSQESLQSLMG"
                     /name="LysR family transcriptional regulator"
     gene            complement(23462..24259)
                     /locus_tag="GBF15_RS25255"
     CDS             complement(23462..24259)
                     /locus_tag="GBF15_RS25255"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-48"
                     /protein_id="WP_015059991.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAT
                     WNRDHNLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATEQISFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-48"
     gene            24478..>24893
                     /locus_tag="GBF15_RS25260"
                     /pseudo=""
     CDS             24478..>24893
                     /locus_tag="GBF15_RS25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<24962..>25474)
                     /locus_tag="GBF15_RS25265"
                     /pseudo=""
     CDS             complement(<24962..>25474)
                     /locus_tag="GBF15_RS25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(25540..26244)
                     /locus_tag="GBF15_RS25270"
     CDS             complement(25540..26244)
                     /locus_tag="GBF15_RS25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            26471..26725
                     /locus_tag="GBF15_RS25275"
     CDS             26471..26725
                     /locus_tag="GBF15_RS25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083830.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEVK
                     VFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
                     /name="replication regulatory protein RepA"
     ARG             complement(23466..24259)
                     /name="blaOXA-48"
                     /gene="blaOXA-48"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-48"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          32..858
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.517"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(26773..27029)
                     /name="IncFIC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.94e-132"
ORIGIN
        1 gtgactgatc ttcaccaaac gtattaccgc caggtaaaga acccgaatcc ggtgtttaca
       61 ccccgtgaag gtgcaggaac gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc
      121 ttcacttccc gttttgattt cgccattcat gtggcgcatg cccgttcgcg tggtctgcgt
      181 cgacgcatgc caccagtgct gcgtcgacgg gctattgatg cgctcctgca ggggctgtgt
      241 ttccactatg acccgctggc caaccgcgtc cagtgctcca tcaccacgct ggccattgag
      301 tgcggactgg cgacggagtc tgctgccgga aaactctcca tcacccgtgc cacccgggcc
      361 ctgacgttcc tgtcagagct gggactgatt acctaccaga cggaatatga cccgcttatc
      421 gggtgctaca ttccgaccga tatcacgttc acatctgcac tgtttgctgc cctcgatgta
      481 tcagaggagg cagtggccgc cgcgcgccgc agccgtgtgg tatgggaaaa caaacaacgc
      541 aaaaagcagg ggctggatac cctgggcatg gatgaactga tagcgaaagc ctggcgtttt
      601 gttcgtgagc gttttcgcag ttatcagaca gagcttaagt cccggggaat aaagcgtgcc
      661 cgtgcgcgtc gtgatgcgga cagggaacgt caggatattg tcaccctggt gaaacggcag
      721 ctgacgcgcg aaatcgcgga agggcgcttc actgccaatc gtgaggcggt aaaacgcgaa
      781 gttgagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgtaatta cagccggctg
      841 gccacagctt ccccctgaaa gtgacctcct ctgaataatc cggcctgcgc cggaggcttc
      901 cgcacgtctg aagcccgaca gcgcacaaaa aatcagcacc acatacaaaa aacaacctca
      961 tcatccagct tctggtgcat ccggcccccc ctgttttcga tacaaaacac gcctcacaga
     1021 cggggaattt tgcttatcca catttaactg caagggactt ccccataagg ttacaaccgt
     1081 tcatgtcata aagcgccagc cgccagtctt acagggtgca atgtatcttt taaacacctg
     1141 tttatatctc ctttaaacta cttaattaca ttcatttaaa aagaaaacct attcactgcc
     1201 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc gaccggagcc
     1261 actttagtta caacacacaa aaccaacctc cagaaaaacc ccgaaccagc gcagaactga
     1321 aaccacaaag cccctccctc ataactgaaa agcggccccg ccccggccca aagggccgga
     1381 acagagtcgc ttttaattat gaatgttgta actacatctt catcgctgtc agtcttctcg
     1441 ctggaagttc tcagtacacg ctcgtaagcg gccctcacgg cccgctaacg cggagatacg
     1501 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctctcat
     1561 ggctgaaagc gggtatggtc tggcagggct ggggatgggt aaggtgaaat ctatcaatca
     1621 gtaccggctt acgccgggct tcggcggttt tactcctgta tcatatgaaa caacagagtg
     1681 ccgccttcca tgccgctgat gcggcatatc ctggtaacga tatctgaatt gttatacatg
     1741 tgtatatacg tggtaatgac aaaaatagga caagttaaaa atttacaggc gatgcaatga
     1801 ttcaaacacg taatcaatat ctgcagttta tgctggttat gctggctgca tggggcatta
     1861 gttggggagc cagatttgtc atggagcagg ccgttctgct ttatggatca ggaaaaaact
     1921 atttgttctt cagtcatggt actgttctga tgtacctgct gtgtgttttc ctggtatacc
     1981 gccgttggat agctccgcta ccggtcgttg gtcagctgcg caacgttggc gtaccgtggc
     2041 tggtcggtgc gatggccgtg gtgtatgtcg gtgtatttct gctcggtaag gcgctggctc
     2101 tgcctgctga gccatttatg acgaaacttt ttgccgataa gtccatacct gacgtgatcc
     2161 tgacgttgct gaccatcttt atccttgccc cgttgaatga ggaaacgctg ttccggggga
     2221 ttatgctgaa cgtcttccgt tcacggtact gctggacgat gtggctgggg gcgctgataa
     2281 cgtcgttgtt gttcgtcgcc gcgcacagcc agtatcagaa cctgctgaca ctggcagaac
     2341 tgttcctggt ggggttgatt acatcagtgg ccaggatcag aagtggtggc ctgctgctgc
     2401 cggtattgct gcatatggaa gcaaccacgc tgggtttact gtttggttga aagttatatt
     2461 tttattaaac attgtgcgtt aaagcctggt gtgttttttt agtggatgtt atatttaaat
     2521 ataactttta tggaggtgaa gaatgcatac cacccgactg aagagggttg gcggctcagt
     2581 tatgctgacc gtcccaccgg cactgctgaa tgcgctgtct ctgggcacag ataatgaagt
     2641 tggcatggtc attgataatg gccggctgat tgttgagccg tacagacgcc cgcaatattc
     2701 actggctgag ctactggcac agtgtgatcc gaatgctgaa atatcagctg aagaacgaga
     2761 atggctggat gcaccggcga ctggtcagga ggaaatctga catggaaaga ggggaaatct
     2821 ggcttgtctc gcttgatcct accgcaggtc atgagcagca gggaacgcgg ccggtgctga
     2881 ttgtcacacc ggcggccttt aatcgcgtga cccgcctgcc tgttgttgtg cccgtaacca
     2941 gcggaggcaa ttttgcccgc actgccggct ttgcggtgtc gttggatggt gttggcatac
     3001 gtaccacagg tgttgtacgt tgcgatcaac cccggacaat tgatatgaaa gcacggggcg
     3061 gaaaacgact cgaacgggtt ccggagacta tcatgaacga agttcttggc cgcctgtcca
     3121 ctattctgac ttgaacatgg ggtttgaggg gcaactggat gaaaacgtac gatttagggc
     3181 actaaaaccg ctgttgtccc accattctgg tgattcccaa acgttatttg gctaaaaagt
     3241 agttttgatg tggtttattt tccgaattcc aagcgcagcc ctactttctt gtgcgtattt
     3301 gcgtgttttg cgcagttttg aagttccggt gatgctgcca acttactgat ttagtgtatg
     3361 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     3421 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
     3481 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
     3541 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaaccg cccgcattat
     3601 gggcgttggc ctcaacacga ttttccgcca tttaaaaaac tcaggccgca gtcggtaacc
     3661 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtgggga
     3721 tacgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag gctccggaag
     3781 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
     3841 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
     3901 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
     3961 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
     4021 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
     4081 gttggagtca ttaccagaat ccccactgcc ggcgagagtt cgctgggaag gaattcaaca
     4141 tgactatcaa tatttccggt aattgacggc aaaatatagg gagctcgttt tgatgttgcg
     4201 ccggcaacat aattccgtat ttttttggcc cgcgttttgt tcgcttctcg ctggctgcga
     4261 tccataacat cgccatcatt gtccattgca agaatacgac gtaacacgac cataggaacc
     4321 gtcagcatgt attgttctat gccgccctgt attccgcgtg tggccgggac tctgaaaaaa
     4381 tagttatcag tattcataat atttccttcg tatatccgat acgttcagga agcacgtttg
     4441 cgcgtcggtg ttttgcgctg ctatttccag atcggcggta agccggcatc gatgcaaagg
     4501 acgcaccaga cggaatacgc gatgtcactt tctccgttca cccagcggcg caccgttctg
     4561 ccgttcacgc cgacatgtcg cgccagtccc tcctgactca ggttgagcac attttccagc
     4621 agatagcgga gttcatcccc cgtcggcggg gtccatttat cagcgggacg gaaacagctt
     4681 tcgcggatcg cgcccacgtt aagcggcacg gtatatttat tgcccatagc aagcctccag
     4741 agccggccgc accactgtgc ggccgcggtt atcaaatcag accatgttca gcaaaagagt
     4801 aaatatcgct gccgcccaca atcaggtgat cgaggacttt gacatcaacc atccccagca
     4861 cctgcgccag acgctcggtg atcagcttgt cagcctggct gggtgtagtg ttagcggaag
     4921 gatggttatg cgccagtacc acggcggcgg cattatgatg cagggcgcgt tgcaggacga
     4981 tacgcggatg aacggtgacg ctgctgatag ttcccttaaa cagttcttca aacgcaatca
     5041 cgccgttgtg gcagttaaga aagaacgcgc agaatgattc gcgtggttct gcagcaagac
     5101 gaaaccgcag taaggaacgc gcggcgtcgg cgccggtgat aaaatccgtt gtggtaattt
     5161 taccggcaat aatggcgtcc gcctggcgga taatgttgag ttcttctgct gaaaatttca
     5221 tatatttccc tcgttagggg acctgagcac catgcccggc cacaaatata taataggaca
     5281 ttttgtccta gatgtaaata cagtgaaata taaaaaagcc cgatttaacg ggcttttatg
     5341 gtctatttct gtccccctgc aataacttcc agttccgtct ctgaaagcat aattttcagg
     5401 cagggtttgg tacggctgag aagttcaatc gacctcggat cgagctccag ggattcgcaa
     5461 aaatcatctc cgccacaggc atcacgcatc cggtcccaga gctcgtcaaa atccgttgta
     5521 tccggattaa aaccattggc gtaacagaca ttgtgcatcc cattactgtt tttctcacct
     5581 ttcgccgcca tcacatacac gccatgatct ttgacaaaaa gaagcgggca attaaaacgc
     5641 ttaacttcag caataacgac gttgagcata ggatcagtaa aaacaagttc tgattgcatg
     5701 gtcatagtgt atttactccc tgaaagctat ttgttgaccg cagcggcggt gagaatgtca
     5761 gaaagcgacc ccagcagata taattcggtg agctcgcctg tttcattcag aataaatagc
     5821 cctgcaggtc ggccattatg aaaaataatg gcttcatgcg attgatgtaa ttcacactga
     5881 cacagcgaga aataaataag tgaccattca ggggaaataa cggttttatt gccatgttcc
     5941 cggatatagc gggcaacatc agcctgttgc tgagaattga gagcagtcat aaggtgactc
     6001 ctgcgccggt cggggcgcta ccgtcgaaag accggattgc ctttcgatgt aattattata
     6061 cgtctaatga gccatataaa tcaagttaat tttgttattt ccctggttta aggagactct
     6121 ttcccctgta aattttgcat cacaagcgca ccgttcgggc gctgcaggcg aataatatcg
     6181 ttctggcgag cgtctttttc ccggatgatc cgccaggttt cggtgacggc gccggctggc
     6241 tgatacagtc tgaccttcac ctcggaacgc tctgcagaca gcacaatgca gcctttctca
     6301 tcttgtgcag ctgcgtgccg ggcttcatct ttttcccgcg gacagtcatc aaatcctgat
     6361 attcggaccg ggtggccatc gatatcaacc tgatagccgg gcgaaatcac gggcacgggc
     6421 tgtggcacat tcgggaaata atcagccaga tcacaccctg tcagggctac gggcacaagg
     6481 caggcataca gcattaacat tcggcgtgtg gtgatcataa agggtcctct ctggtcgccg
     6541 cgggccttat ttcgccttct caaccagctg cagtgacgga tgctcaccgg ccagaagtaa
     6601 caggaaattg aattccccgg tcttgagctt tacgccgcgc tgagcagcct tttctttggt
     6661 ctgccatgac gccggtttat agccaaacag cgctgcaatt tcagcgtgct tcagccccag
     6721 cgccagacgc aggcggcgaa cgtcttcggc cgtggcggga tctttgacca tatcatcagg
     6781 actgaatgcc ctgttcaggc ggtaatcagg atgcacgccg gcgatcagca tcagcatgtt
     6841 atattcgcct gggcgaagac tggtcttgtt gtactggctg agatcgtcgc tgatagcttc
     6901 cttacgctgc caggcgcgca gttcgtaccc catgacagcc cctaatgcgg tttcagtcat
     6961 gcccattttt tcccggagcg tcttcactgt ttccggctgc ggaacgacgc taaccatctt
     7021 aatcattacc cttctcccag aagtctgaat tcagaaaaat tttcacctgg cgcgtgcggg
     7081 gatagtcctg gtgcttcagc ccctcccgcg tgtcagacag gtactgcgtt tcctgctgca
     7141 ccttgttgat tttgccattc catttgcggg ccagcgcccg gtaactcccg ttctccagct
     7201 gataaacttc aaacgtatac ttgttcggac cgctccagcc ttcgttgtaa tagacctgtt
     7261 tcataggatc tgcctgatcg atgcgccccg taaacatgac atcccggctg cattgcctga
     7321 accagtaacc gcggggcata gccggtggcc ggtcgcttat ttcccgtctg gatagcgtgc
     7381 cgaaaggctg tgagcgcagg tgacaacctg atctcaggtc aatctggcat ggcgtgttgt
     7441 gcagggacca gcgccgctgc cgcagacaat ggctcagggt gatcacagga acgacacggg
     7501 aaacgaacag ggggaatgat tgcatggttt tactccttcg ccagtcgggg cgctaccgaa
     7561 aaatgagcct tgataatacg acgaatgagc catataaatc aagttaaaaa tcaggcggca
     7621 gagtttctgc cgctgctgta ggtcagtacc gtggagccac gtcctttcct tcgtcccata
     7681 aacgggaaaa ctcgttcacg taggcttcag ttactgcagg cgcgccgctg atcagcacca
     7741 cattttccgc attacgactg tctgcgctgg cgctgaagtt aaacgagccg gtcaatacgg
     7801 cgcggcggtc aaagaccatg aatttatcat gcatggcctc gtagttatcg ttgatccgca
     7861 ccgccacgcg gttattggcc agaaagttca cttcggtgta ccggttgtcg ttcgctttgc
     7921 gatcggccac caccaccacc gatacgcccc gtttttttgc ggcacgcaac gcgccggcaa
     7981 caggacgact ggtcaggctg tacgcggcaa ccagaatctc atgctgcgcg tggtcgattg
     8041 aatccagaac cagatttaat gcggtcccgc cgggtgaaaa cccggttttc acttcagtcg
     8101 cagcttgcgc ctggacatcg ccggcaatat tgccggccgc tgttaccggg tttgctgcca
     8161 cggcgccggc gatgagggtc agggttaaca gcacatattt tttcacgcag cccccttaat
     8221 catcatccgg gaacagatgc tggacgttct tacgggtttc ttccagattc ttatcctccg
     8281 gtgctgccgg ttcgggagcc acggcgggac tgttttccgg cgcagcgccg cctgccggcg
     8341 acaccgtatt gctgccaccg ccggtggcct gcagaaacgc ctcaaccaga cgcgccattt
     8401 ctcctgtatc ctgctgcggc agcgtactgc tgatgatccg ctgaatatcc tgcatcgtgg
     8461 tgctctccgt caggaattca gcgatcagga acggcaggcg ttcgtcctgc ttcatcaacg
     8521 cggcaccaca gagcatcatg gcgcgcatcg cgcggccacg ctctttctga ggcagtttct
     8581 caagaaagcc gtcggctacc cggtccgccg cattgcgctc cgggtagata taaaacgagc
     8641 acttacggtt atcagtcata ctcagccctt cattgccacc atacccagaa ccagatcgaa
     8701 ttgcgggtta tcactgacga agaaacgatc gtcactcacc cccgtggcct gcttcactgc
     8761 gccggcgacc aggctggcac cgccaccgac aaccatgacg tgcgtgaagc cggcaaagcg
     8821 gccaactgaa tcggtgacac ggtgcgtcag ggctttttcc cgctcatgca gtaccttcat
     8881 cacctgggcc cgctggtcag cattctggag gtaacgggaa agaaacgttt cgtcatttcg
     8941 cgcctggatc agacggtcgg caaaatacga actggtacgg gtgctcacac tggtatccag
     9001 tgcatgtttt acggcttcgg tgatgatgga tacgccggtt ttaggatcgc aatgcgtctt
     9061 ggtgaatccg gacattttgc tgcgaacgtg cgcgatatcc agcgtggttc caccgatatc
     9121 cacaatcagc agggagtcca gatcgtccag atccttcagc acactgaagc ctgcagggac
     9181 cgactctggc agtacgctga ctttacgcac ggtaaagctc tcacgcccct gaacggccac
     9241 cgcgcgcttc acgttcgctt ttttccgctc gatgttggcc atattaggct gatcgttttc
     9301 atccaggtac tcgcccagcg gcagcgtcac cacaacatca accggctgcg gttcaatgcc
     9361 agactgcatc agggcatggt ggatagcgac gacattcaca tcactgtact gatagcgtgt
     9421 ttccgtggtg cgcacggcat ccgggctcag ctggtcaaag ctgtactttt ccccgtcaat
     9481 ttcatagttg gcggaagccg gcaccgatga atctgtcagc aatgtcatgg accattcggg
     9541 cttgaagctg ttcggactga tcaacgtttt cactgcgcct tcttccatcc aggccagttt
     9601 gatgttggtg gaaccgtcat caacaaaaat acgcattttg aacctcacat tatttagaaa
     9661 aaaaccgtag aaatcaggaa aaatacagct ttcaaatgga ttattacgca tctctgctga
     9721 cgtgtaaaga caaaaagatg caccaatata gaaaaataca gaaaaatacc gaataaaaat
     9781 caacaaaaga cggtcagacc gtcctgaaaa cggtctgaca tagaaagaaa agggatttat
     9841 ttagatttat acggaaatat cagggaggga tacagattat tccgggagcg ttaaatgact
     9901 gtctggcagg ctgaatcgga cgccatactg acgaccaaca tcgagcgcaa acacgcgcgt
     9961 gtaggtttct gtggtttcca gccgggaatg cccggcataa gcctgtacaa ctttcagcgg
    10021 aaccccatgc tggatcaggt gcatacagaa gctgtgacgg aacgtgtggc aggtaacagg
    10081 gatactaaaa gtgacagagt cacgttttgc gaggtccagt gccttcctga tccagttacg
    10141 cggcgtgtta tcactctgca ccggccagag cagctgctgt ttaccaatac gcagggtggt
    10201 cagaaattca cgcaccttcc tgacatattg aggatcggtc agaggaacaa gacggaacac
    10261 ctcttcatcc ttgcccggcc tgccgcggcc acgctgacgc tgtttaagcg tcttcagccg
    10321 gacaaacggc cgggaaccgt caagctgaaa gctggcgccg gtcagcgcca gcgcctcatt
    10381 aatacgcgcg cccgtgttcc agagggtatc gaaaaggacc tgctggcgca ggtctggcac
    10441 ataccgcaga aacaccgaca cctccggggc cagcaggtat ttgggtaaat cagcctggct
    10501 ggccatctcc cgcagcgcca gcgcatcggc ataatcgaag taggcatatg tcacgcgccc
    10561 ggtcccgatg gcgtcgctac gtgaattctg ggaaatttgc agggggaaag tgttcaattt
    10621 tttaaaatcc gctaatcagc cgtcaggcga ctgaatttta tgaaatttct cgctgaattc
    10681 gaccgcatcc tgcatatcaa acgtcatact gcgggtggag gcggtcgagc tccagctttt
    10741 cccgccttcg attctgtaag ggggtagggt cagtccgcgt aaaggcttgt catgccgggc
    10801 tgcaaaaacc agctgcccgg cagatacttt taacatcata gcaaacatat ccgcgctgat
    10861 tcggcggcct ttcaatccac tcataacaac ctgtaactga atcctgtacc acgacttttg
    10921 ttgtaaatct gctgaccaga ctgaccttca tcttcactga ataccaccat acggcggccg
    10981 ttagtcagtg aatccatttt acgctgtgcg ccattatcgt cataacgcac aggcaggcac
    11041 gcacctttat ggctgttttc actctcgacg cggaacaccc ttaccctgac ggcgttcttc
    11101 cgcacctggt caggatcttc accactgacc acgcccgtca gtttctgaag cgtatctgcc
    11161 ggactgctgt actcagcctg gctgagatag cggccgttcg gggttaacca gaaatcctgg
    11221 ctgtcactga cacggtaggt ttcgccgtgg ctggcgatgc ctgagacgtt acagaagcgg
    11281 tcataatcgg tgggctgtcc ggggagcatg gcgcaccctg tcagcagcag tgccggcagg
    11341 gcggcgacgt atcctttgaa tttcatgttg tctcctgttc aggatgaacc atttcggtcg
    11401 gtggccggta tcccggatac gggataaacg gcgcatcggt ggcaataagg tctagcagct
    11461 tctggccact gaccaggatc atgcggctgt ctccgcgaat gagcgatttg gtgccttcgc
    11521 gcgttttgcc ggtatggcag aaaaaaccgc gggcaccggt ggcgttcagc aggtcgctga
    11581 agtcacggac gtggccaacg gcaatatagc tggtatagcg tttggcctgg ataagccagg
    11641 tctggccatc aatcttcaca cggccatcaa tcccgccgtc accggtatac cggcggttac
    11701 ggatcacttc aaagccccgg cgttcaaagc cttcaagcag cagctcttcg aacacaaacg
    11761 ggtcaatctt gcgcagatag gcaattttct gcccgaaatg cggaaacgta ttaattttat
    11821 cgatcacacg taccgcgctg gcctgtttcc gttcgtgccg gcgccggcgt cgccgtgtac
    11881 cctgccagag gccatacagc aggatacccg caaggataaa agagccgaca acatggatgg
    11941 actcattcat tatcgatcct cagcacttca tacttcagct gccgcagcag cctgtctttg
    12001 ctcatggttt tcagggtggg ccattccgct ttcagcgtca gcgcgacggt ggcaagttgt
    12061 atggtccggg gtactgtctc accgtcctca tattttacca ggctggtacg gctgataccg
    12121 agctcagccg cggcgcgttc ctgggtccag ttcaggcccc gtcgccacag gatcaagtca
    12181 aagccggtca atccgccgca ctcccatgct cagtgccggg cttcgtgccg gcgggtacgg
    12241 cctctttccc ctgtgccttt ttttccgccg gggccgggta ggccgaggcc agttgtgccc
    12301 ggaaatcacg ccacgctgac agccagagcg catcagatac ctggtcagac gacagattaa
    12361 ggaccgcatt gcggtcacgc cagtggctaa atgtctgagc cggaaggccg gcacgccggc
    12421 gcaactgctg ctcgtcgaaa taggcacact gaaccaggag gtgtctgacg ttctgcggga
    12481 caacttcatc tctggcccct tccggcacag ccagcatccc ggcgctgcat ataccctgaa
    12541 tgcccgcctc ggtataaatc tgctgcagat tggtatcatc aacctgcgca gcgccggcgg
    12601 tgtgcgccac aaccagcatg atgccggcaa taagtggttt ctttatcatg cgtactcagc
    12661 tcctggccga atcctggtgg actggcctgt tataatctgc ctgtatttat agcctgaaaa
    12721 tgttcatatt tgaacatttt ttatgtatat tatttccgca aattcgccat aaacacaaca
    12781 ggagacgcga tggatatcaa ctactacgat aaacatcagg aagaatttga ggcggtcacg
    12841 ctggcgctga aagccaatct ggaagaagtt tggggcagca gcctgaaaaa tcagggggaa
    12901 agcctggatg atcaggtgac gtatatgaag ctatttgaag agctgcaata caacctcaat
    12961 ccgtactact tcaaggagaa cacctcagca aaagagatgg atgaagataa agtagcggcc
    13021 tttgtggcca gaacccgcga ctataagcac ggcattacca ttaaatcctg gccgggacgc
    13081 ccgcagaaat ggctgaaagg ccgaatcaaa cctcttcatc ctgtggaagg cacaaacctg
    13141 tgctggatcg acacgtccaa cattgtgcat atcggtgccg ataggcagtt tgacgatcaa
    13201 tattacctca ccgtgaccac acagaacggt cagagttacc gcgtgaatga tgtccttctg
    13261 cctggacgcc ttctggatgc cgctcatgaa gccctgttca gggcgctgga ttcaagcact
    13321 ggcggtaatt tctgatacgg agttgcacca cgtttatcgg gtacttatac tgacagtgat
    13381 taacaaatta attttccgtt tactccgaca gaaagttaag tagtaaaaac gtctagtaat
    13441 taaaaggagc ccttcctcac cagttgggct ccttttgtat atccttattc atttcatcaa
    13501 atgtcggtcc gagaactgtc cgcggccagt ttctgaattg cactgatctc atctccggtc
    13561 agctcagcac gactgtccag ctgctgcacg tttttgctcc ggacctccca gaagtagtac
    13621 aaaaagaatg cctcgcatgt tcatgactct tctccctgtt cgttttcacc tgcccggtat
    13681 gcccgctgcc ccttataacc cagcagggat tcgcacatca ggtcagtgat gcgtgcaata
    13741 gcgagatccg cattgtcagc acgcagggaa atcacggccg tttcgccccg gttcgcggca
    13801 ttccagacgc caggcagcag cgtctggaac cgggcccggt catccccctc cgacagcagg
    13861 gccatttccg gtagccgttc gcggatcacc gcttcactgg cctgccgcag gatatcgtca
    13921 cgactccaga tgcacagcgt tgccggtccg ctgcgcatga ggatagtagc agcgtcagcc
    13981 atactcagtg tgtccagctt cagcatctcc atgcccggcc gacgcagcac aaatcctatg
    14041 ggccggcggt acttgcccag ctgcagcaga tcgtcgtctg taagcagctc caggccacgc
    14101 tgcagaacca gatgaagcac gtccgcttct ttgaccgggc ggatcaggtt ttgcctggca
    14161 tttgcccgga cggtcagaac ttcaatacga tgccagagct cgtcattaat atgcttggtg
    14221 gccatcaatc ctctccttta cgggcacgaa tataaatcag cgtcacaacc acaaccgcgc
    14281 ctatggccca gctaatcgcg gagtaaccct ccggcatgta gatcatgacc agcattgcca
    14341 gcagtaaata tataacggca gaaatcaccc cctgacgcat cataaccccc tctctgttaa
    14401 acattgttca ctggccaccg gaatgctccc cgccgacata aaaccgtttt ccatccgccg
    14461 ctttgcccag gcatacggtt tgacctgtgc ttagctggcc ggcatccatc atgaacggcg
    14521 cgcccggacc gcgaaaaacg tccgtatcga gggcggcagg ttcgccggca gcgaccggcc
    14581 ggaccatgcc catatcgtag ctgtccggca ggttatccgg caggcaggcc tgcgtgtagt
    14641 cgtccgcgac cttctgcgcc tcttcctgcg tggggtaacg acccagcacg ccggtgatct
    14701 ctttcttcag tccgaagccg tagccgcgaa ccttgtacgc ttcatattcc ataccattca
    14761 ctccgcttgc cgttgtttac tgaataacca cactctaata aattaggttt cattcgtata
    14821 ttataaatat ttagatttag cactaaaagc taatttaata aagcaacaca ccggtaatca
    14881 tcacgctgcg tgacgaccgc acgatttacc ccgccggatg atttgcgccc ttatcttttc
    14941 accctgtgac ttttacgccc gcaaccaccg gaaaatacct tttggccggt gccgccattg
    15001 cggaaaaaac gtaaataccg gaaaaaacga aatcaacagg gagaggaagg caggggaacg
    15061 gtcgcgcggc tgccggcgcg gacaccatat cttgtgccgt ttccggctga cggagccact
    15121 acatatagcg ttatccacag aaacggtgga taagtggcgg cgctgcggag aataccgcta
    15181 aaccgcaacg gcgaaaaaat gtggtcccgc gcgcaaaagt gcggtacaaa tggctgtaca
    15241 ttatgtaaag ccagatgact gtacaaaaag gaaggagcat gaccggtttt tttgaacgta
    15301 aaaagcgggc caaaaacgcc cagctgaccg tgcgtctcga tgagcagctg aagaaacgcg
    15361 ccgaactgcg tctggaggca atggggatga ccgccacgga agcggtggag cagctttacc
    15421 gcttcatcgc cgatcacggc cggatgcccg tcacggagcg tatcgtcaca tttgacatcc
    15481 agtcacagcg cggccgcgcc atcgccctca atcccgccga cgctgtgacc gaaaacaccc
    15541 ggcggtttct gcagtcgctg ggagctgtgg aagtggtctg ctgcgatccc gctgcatttg
    15601 aacaggcggt actggagtac ttcagcctgt atgtggccga ccaggctgcc tgtttactgg
    15661 ccggtgtact gaacgtcacg ttcatggtta atgccgccag tcgcgtgaaa gatgactggc
    15721 atgacaggct gctgtccctg ttacagcgcg actttgattt cagcgaagaa cagtcagtga
    15781 cgttcattaa cagcatcgta gacagcgaac atgtcgatcc tgccatcgtc agtcgaaata
    15841 atacggcctg gcggagcgga aaaagcaccg ggtccggcgc cgccgtcctg cagcgtttcc
    15901 tgacgttcct ggcggagcgg ggcgtcagct gcagcgatcc gcagagactg accgaaagcg
    15961 ccggccgtgc atggggcgtg cgtgaaaata tgatcgggaa tatgcccccg gacatgcagc
    16021 aggccataaa cgatggcgtc atttttgagg ccagcgctga cacgccggac ttttcccctg
    16081 agctggcggc ggcgtttggc ttcacacagg cccaggcaga agcggtgctg aaacccgccc
    16141 tggccgccag ggatctcccc ggtttcctgc agcgctttct ggcgttcctg gccgaccggg
    16201 gcatcagctg cagcaatccc cggcggttta ctgaaaaggt cactgaacac gccagccatg
    16261 aggggctgca ccggcttatt gcgatgttga tgtcgcgcat cagggatgag ggggtctgtt
    16321 ttgtctttca caatgaatcc ccggacgaac tgccgcgcct ggccgcgtca ctgcccggcg
    16381 aactggccga taccttcggg tttacgggat ggcaggcaac aacaatactg cgtcaaatcc
    16441 agccggcacg gccggggcag gattagggcc gggagcctca cgcgatgaaa acgacctgtt
    16501 atgcggacgg catcacggcg gcgccacttt ccccgcagga gtggcgggca ctgctcaggg
    16561 cgaaaggctg gaagcagaag gatctggccc gccgctggaa tgtgtcagaa acctggatca
    16621 gtaagctggc caataacccc gatcggggca cgcactggaa cgatgcctgc cggggtctgc
    16681 ccgatatgcg tgcccggcgg cccggcgaac cgcgcgcggc aggcacagac gcaccagagc
    16741 cgggaggccc tgaatgaaca aacgtcttca cctcatcacc acctgtaccc ggcgcaaggc
    16801 gggcagcgtg gaggataccg tttttccggg cgacacttcc cttgatgacg cctttgacgg
    16861 ctggtacagg ctgatcgccc aggccgcccg ccgtcctggc agggtgctgg aaacgggaga
    16921 gctttaccgg ggcgcacact ggctgcgtgc ccgcggtatc gcggcgcggt ggccgggcat
    16981 cgagctgtgg gccatgtcgg ccggtctggg gctgcgccac cataccgatc cggcggtcgc
    17041 gtatgaggca acgtttcacg ccatgccctt cgcgccggcg accgtctggg gctcactgac
    17101 ggcacgcccc ccgctggcgg gccgctgcgc ctccctggct gagctgatgc aacgctgccc
    17161 ggacgatcgc tttgtgatcg ccggttcacc cgtgtatatc agcgcggctg agcaggatat
    17221 tctggccggc gtgccggcac tgcatgatgc ggccgctcag ctgattatcg tgacctcaca
    17281 gggctatcgt ggtccgctgc agccgtttct taaaagaagc cgcgcggaca tgatggcgac
    17341 cctgaaatcg aacatgacgt gcctgaatat tgcctgcgcc ggcgccctga ttgacgccat
    17401 gatgcaggca gacgcccggc aggcggcaac catttaatac agacagagac tggcgctacc
    17461 gtcgccagaa gggggtttta catgttcagc atcatttatc acgccggcgc agccgtcctt
    17521 ttcctggtca tgagcctggc ggccggcgcc ggcctcctgc ttcatagcca tgaatatacg
    17581 acaggtcatt tctggaacat gacggggctg tgcattgtgt caactctcgt ctggatctgg
    17641 gctgtagctc aggcaaaaga agcctggtac atttcacgaa acatcaaaaa aggactctga
    17701 ccatgaaaga tgaaattcgc cacgaaaaac ccgttgaagt gaatatacag ctgacacaca
    17761 gagaagcgca ggctctggcg caactggtta agcgtctggg attcagtgac tgccgcggcc
    17821 tggcgacgtc agatattgag gcatatctga tgatggatgg aatcaaccag atcatgaaag
    17881 cactggctga agagggctat gcaccacgat aagggccggg actatctgga attagccgga
    17941 aatacggccc tatcacgcgg gcagtaagga gaggttttaa tgttcccgac accagggaaa
    18001 acaagccgcc gtcaagagcg gctgcacatc gctgttatcg cataaacgcc gcgggaatat
    18061 ccttccactg cgtggtgtag gccggggaca acatttcacg gcgcatggca tattccggat
    18121 caataccttc tccggcgaag aagatccttg accttccttc cctgtttatt ttgtcgatgg
    18181 tggccatcag tttttcgctg cccggccgca cagcagccgg cgaaaacaga tccagctggg
    18241 tttcatatcc ctgcagatcg ctgagcatca cgccggcttt tgcataccgc acgccctcac
    18301 gccagatacg cgacagtccc agctgcgccg cggcgatgat gtcgcgggta tcctggcttg
    18361 gcacgggaag cacttccgta gcctggttac tgtagggcac accgccggcg tcataagcac
    18421 tgctgcggat aaagaccgtt accaccttcg ccagacggtt ttcctggcgc aacttttctc
    18481 ctgcccgtgt ggcatattta gtcaccgcct gctgcatctg cgccagttgc gtgacccgcg
    18541 atccaaacga acgggataca acaagctgct gtttcgcgcc ggtaccttct tccagggcaa
    18601 agcaggcctc gccacgcagc tcacggatgg tgcgttccag cgtcacaccg tacagccggc
    18661 gtgccagtcg gatatccatc tctgcgagcg ccagcgccgt tttgatcccc attgtcgcca
    18721 gccctttttc ggtacgatgg ccaacccccc acacatcgcc ggccgacacc aggctcatca
    18781 gccttttcag tcttacagga tcactgagcg ccacaacccc cccggtggca ggccacgtct
    18841 tcgcggcgtg gttgcacagt ttggccagcg tcttcgttgg cgcgacgccg actccacagg
    18901 tgagcgttgt ctggcgcagc acttcagccc gcaaaagctg gccaaacgcc tgcggcgtca
    18961 tcactccctc catatgagag gcatccgcaa agagctcatc aattgagtaa acaaatgtcc
    19021 gcggcgccag actctccacc acggttgcaa atctggcgga gaatgaggcg taaagggtgt
    19081 agttggaact gaatacggca acgttatgct gttccagcag gcggcggcag cggaaatagg
    19141 gctccccgcg gcgcacgcca agcgctttcg ccgcgcgatt gaccgcaaca agcgccccgt
    19201 cgttgttgct gagaacgacc acagggcgac ctcgtaaatc agggcggaac gcttcttcgc
    19261 agctgcagta catcgaattg acatcgatta aggcgtacac gggcggtgtc tcatgacagt
    19321 gaacgtaacc acaccccata tctgcagcgc ctccggatca aacctgattg gcggaaaagc
    19381 cggattcagc ggcatcaggc aggggtaagg gcggagctgc agctgcttga ccgtaaattc
    19441 attatcgatg ctggcaatca cgatgctgcc gtgccggggc tcttctgccc gatcgacgac
    19501 cagcacatcg ccatcatgaa tgcgggcatc acgcatagaa tcgccggaca cacgcaagaa
    19561 gtacgttgcg gccggacgcc gcacgcagta atcatgcagg ttaagttcat tggcctcata
    19621 gcctgcggcc gggctgggaa agccggccgc aacgggctcg gcaaaaaaag ggagtgcgtc
    19681 accttccact gaaggccggg gataaacgag atccagcgcc ataatttagc ctccaataac
    19741 tgtttataca tacagtatat tgcaggcttc aggaaacagg caatgccggc atatttcatg
    19801 ccggcacgct ggggtcaggt caggatggtg gccagacggc caagaacgtc gtccatgata
    19861 gtctctggca cccgttcgag tcgtttgccg ccgcgggctt tcatatcgat cgtccggggt
    19921 tgatcgcaac gcacaacgcc ggtggtacgt atgccggcgc cgtcaagcga cacagcaaag
    19981 cctgctgtgc gggcaaaatt gcctccgctg gtcacgggca caacaacagg caggcgggtc
    20041 acgcggttaa aagcagccgg cgtgacaatc agtaccggcc gcgttccctg ctgctcatga
    20101 cctgcggtag ggtcaagcga gacaagccag atttcccctc tttccatgtc agatttcctc
    20161 ctgaccagcc gccggcgcat ccagccattc acgttcttct gccgagattt cagcgttcgg
    20221 atcgcactgt gccaacagct cagccagtga atactgcggg cgtctgtgcg gctccacaat
    20281 cagccggcca ttatcaatga ccatgccaac ttcattatct gtacccagcg acagcgcatt
    20341 cagcagtgcc ggtgggacgg tcagcatgac tgagccgcca accttcttca gtcgagtggt
    20401 atgcattttt cacctccata aaagttatat ttaaatataa cattcacaaa aaaacatacc
    20461 agcctttaac gcacaatatt taataaaaat ataactttca gcccaacagt aaacccagcg
    20521 cggtagcttc catatgcagc aataccggca gcagcaggcc gccacttctg atccgggcag
    20581 ctgatgtaat cagccccacc aggaacattt ctgccagggt cagcaggttc tgatactgca
    20641 tatgcacggc ggcaaacagc aaagacgtta tcagcgcgcc cagccacatc gtccagttgt
    20701 acctcgaacg gaagacgttc agcactgaag gatcccctca taaatcagcc atagcatagc
    20761 catatttgag caactgattg gcgaataata cccaggccgc cagcaattct ttctccgtga
    20821 tttgataatc aggccgcctg agtacctcca atcccaagcg aatcgttgag agtaccgttc
    20881 gatgcccaat ggtattggcc tgaaaatgct tatcccagcc ctgttgctgt gcgtgtaaac
    20941 caactagcca caacaggcat tgaaccatca acgctatcag taggatgatg tcgaatcgct
    21001 ctggactgtt ggtacgactt tggcgtaagc caaagccgta tgccgggctt ttgaggtcac
    21061 gaaaggtttc ctctatttgc atacgtttgg cgtacaggtt taccagctgc tttgggctcc
    21121 gagattctgg tggtaaattg ctggctaaca cccaaggctc tttcgccgat gttgagtacg
    21181 tttttgccga tggatggtgg caattagtcc tcgttgaacg ctgatttgta cgccccttag
    21241 gtttgctacg ataaagcgtg agatggcaat tgatagggtt agttttggtc aaagtcttac
    21301 aacccaggct cttcgcttga ccgttggcca agtcatgtgt acttcgaact gcacgccaat
    21361 tttcagcgcc aatatctgca aattgaactg taccgcgaat acggcttagc caaaaccaac
    21421 catgcgcctc aacctctttg taccaaggca ctttgaaccc cgcgtcagtc acgatgagag
    21481 gggtaacatg caaaggtaaa atcttggcta agtccgctaa aaaaccgttg tgggaggcct
    21541 tagagcattg ttctgatagg ggataggact tctcatacag ggtaatagag cggccattga
    21601 aggctattga ggcgcgcaga gccatgatgc gtttatgctc tcggatgtct gaccaatcga
    21661 cgagcacaat cggcataggg ttaccagaac agatgaggct ggcatgccat tggtagacag
    21721 ctaaccgttc ttgatgcaga tgcgtgttgc cgagcaaacg atcaatgcgc ttgatgttgt
    21781 gcttggttcg ggccgtggtt ggtaaatttc gacctaactc agtcaaggtc agtgtcttac
    21841 attcaatcag cgccttagag gccagcatca agctgtttaa tcgttttagg tgaagttctg
    21901 ggcagtattg gtgtaaagaa tgttgtagga tattggcttc gcgcatcttg ttgtccaagt
    21961 gtagtttttg gcgaaaccat ttgatcatgc aacaagatgc gcttccaccc taatttgatg
    22021 atttatttgt aaatcatcag gggattcttc agtgctcggc ctgtaaaaaa agcggatcgg
    22081 gcgtttgggc ttgagtcatg tgtttgggct gattattgct ggtcatcaaa agatcctctg
    22141 ctttagcctc tgaaaatttt tagccaataa atataacttt gttatcccgc caaatgccat
    22201 taaactgcat aagacgctat gttccaaata tgagacagtg ggaaaaacat gccagattta
    22261 aacggtatga tgctatttgc agccgtagtg agagcaaagg gattctccca agctgcacgt
    22321 gaaaccggcc acccaaaatc caccataagt cgcaaaatcg cgcagcttga agaacaacta
    22381 ggcgtgcgat tgttacaacg cgacacccgt aacctgagtc ttacccaagt cggggcactc
    22441 ttttatcaac actgtgattc cattcgcaat gaggtggaag cggccaaggc tgttatcgag
    22501 agtacccatg atgatgtatc aggatcatta cggatagcca ttccggtctc ttttagccaa
    22561 gaattaattg ctaacctctg tagcggattc atgcgtttat atcccaatgt ggaattagat
    22621 gtccagttta ccgacaacga tataggtttg gtgggtgaag gatatgacat agctatcaaa
    22681 tatgggccac tgcaatcctc tgatcttgtt gcaagattac tgttcgagcg tcaaccaatt
    22741 ctggtagcaa gccctggcta tttaaaagca cgcggcaccc ccgcaacacc taaggagctg
    22801 agcgatcata gtggtattct gctaggaaca tcccgctcag cacccatatg gcccttaggc
    22861 aaaggtgctc gcaaaaccat ggtaaacttt cagcgtaaag tcagggtcaa tagcccgatt
    22921 atggttaagc agttagccct cgatgacttt gggattgcaa tgctatcaaa ctccgcctgc
    22981 aaaaccgaac tcgccaacgg ccagctagtg ccaatcttac aggaatggcc gattgaaccc
    23041 tttaaggtct acggggtgta ttcgagccgc agacaattag ccaccaatat cagcgccttc
    23101 ttggactttt tcgtcaaacg ctttagcagc caagaaagcc tacaatcctt gatgggctag
    23161 gcactttatc gcagcggcat tcacctttac tgacatcgcc attcctttat acacatccca
    23221 atgttgagtg accatgacca tttagataac aaaaaagcct agcaaattgg ctaggctttt
    23281 tacgttgagg aatgtgatgc taatgctcag gctaaaagtt aacgcgcgga gcttaagtcg
    23341 attaggccta accgattaag tatgagccat taactacaag cgcatcgagc atcagcattt
    23401 tgtccatata tctttacagc ttatatgacg gctatttcac acaaatacgc gctaaccact
    23461 tctagggaat aattttttcc tgtttgagca cttcttttgt gatggcttgg cgcagcccta
    23521 aaccatccga tgtgggcata tccatattca tcgcaaaaaa ccacacatta tcatcaagtt
    23581 caacccaacc gacccaccag ccaatcttag gttcgattct agtcgagtat ccagttttag
    23641 cccgaataat atagtcacca ttggcttcgg tcagcatggc ttgtttgaca atacgctggc
    23701 tgcgctccga tacgtgtaac ttattgtgat acagctttct taaaaagctg atttgctccg
    23761 tggccgaaat tcgaatacca ccgtcgagcc agaaactgtc tacattgccc gaaatgtcct
    23821 cattaccata atcgaaagca tgtagcatct tgctcatacg tgcctcgcca atttggcggg
    23881 caaattcttg ataaacaggc acaactgaat atttcatcgc ggtgattaga ttatgatcgc
    23941 gattccaagt ggcgatatcg cgcgtctgtc catcccactt aaagacttgg tgttcatcct
    24001 taaccacgcc caaatcgagg gcgatcaagc tattgggaat tttaaaggta gatgcgggta
    24061 aaaatgcttg gttcgcccgt ttaagattat tggtaaatcc ttgctgctta ttctcattcc
    24121 agagcacaac tacgccctgt gatttatgtt cagtaaagtg agcattccaa cttttgtttt
    24181 cttgccattc ctttgctacc gcaggcattc cgataatcga tgccaccaaa aacacagccg
    24241 ataaggctaa tacacgcata acgtcccctt gcttaatgca atatactgaa gaatcccctg
    24301 atgatttaca aataaatcat caaattaggg tggaagcgca tcttgttgca tgatcaaatg
    24361 gtttcgccaa aaactacact tggacaacaa gatgcgcgaa gccaatatcc tacaacattc
    24421 ttggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
    24481 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
    24541 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
    24601 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
    24661 aatggcgttg gatgccgggc aaccgcccgc attatgggcg ttggcctcaa cacgattttc
    24721 cgccatttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
    24781 tcatcgtctg cgcggaaatg gacgaacagt ggggatacgt cggggctaaa tcgcgccagc
    24841 gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattggggt
    24901 ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct gacgcctctt
    24961 ttaatggtct cagatgtcct ttggtcacca gttctgccag cgtgaaggaa taatggccga
    25021 gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca tcattcagtg
    25081 tttcaccctg cgcccggaga tgatccaggg ctgcctgcat ataaatagtg ttccataaca
    25141 cgacggcgtt agtgaccagc cccagtgcgc ccagttgatc ttcctgaccg tcggtatatc
    25201 gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaacggca tggcgacttt
    25261 ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca atataattaa
    25321 gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc agtccggaag
    25381 gacgttcact tttcagcaat gaacggacca gcactgaagc ctgtactttg cccagcttca
    25441 gggagccagc ggtccggatc atttcgtccc actgaaggac tatttttggc actgttgcaa
    25501 agttagcgat gaggcagcct tttgtcttat tcaaaggcct tacatttcaa aaactctgct
    25561 taccaggcgc atttcgccca ggggatcacc ataataaaat gctgaggcct ggcctttgcg
    25621 tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa gccgtcttca tggatttaaa
    25681 tcccagcgtg gcgccgatta tccgtttcag tttgccatga tcgcattcaa tcacgttgtt
    25741 ccggtactta atctgtcggt gttcaacgtc agacgggcac cggccttcgc gtttgagcag
    25801 agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg atgaatcgcg ggatctgcca
    25861 cttcttcacg ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg
    25921 ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc
    25981 ccagcggcca ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg
    26041 gttacgccag taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg
    26101 gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg
    26161 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
    26221 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
    26281 tcaccaccga ctatttgcaa cagtgccctg aaaaccatac tatttcacag tttaaatcac
    26341 attaaacgac agtaatcccc gttgatttgt gcgccaacac agatcttcgt cacaattctc
    26401 aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg
    26461 aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcatctggcg caaaacgagc
    26521 atacagaaag gggaatccgc tttctgatgc agagaaacaa agattatcag tggcccgtaa
    26581 aagagcttcg ttcaaggaag taaaagtatt tcttgaacca aagtataagg ccatgctcat
    26641 gcaaatgtgt catgaagatg gtctgactca ggctgaagtt ctgaccgcac tgataaaaag
    26701 tgaagcgcaa aaacgatgca tgtgatgatg ggcttacatt cttgagtgtt cagaagatta
    26761 gtgctagatt actgatcgtt taaggaattt tgtggctggc cacgccgtaa ggtggcaagg
    26821 aactggttct gatgtggatt tacaggagcc agaaaagcaa aaaccccgat aatcttcttc
    26881 aactttggcg agtacgaaaa gattaccggg gcccacttaa accgtatagc caacaattca
    26941 gctatgcggg gagtatagtt atatgcccgg aaaagttcaa gacttctttc tgtgctcgct
    27001 ccttctgcgc attgtaagtg caggatggt
//
