LOCUS       NZ_CP045266             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 16HN-263 plasmid p16HN-263_3, complete
            sequence.
ACCESSION   NZ_CP045266
VERSION     NZ_CP045266.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10353651
            Assembly: GCF_011066505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     Complete sequence of Klebsiella pneumoniae strain 16HN-263
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Kowloon Tong, Hong Kong
            999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:23:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,763
            CDSs (total)                      :: 5,641
            Genes (coding)                    :: 5,476
            CDSs (with protein)               :: 5,476
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 71 of 165
            Pseudo Genes (incomplete)         :: 104 of 165
            Pseudo Genes (internal stop)      :: 16 of 165
            Pseudo Genes (multiple problems)  :: 25 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045266.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-263"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-263_3"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            complement(join(5416..5596,1..365))
                     /locus_tag="ELO34_RS29305"
                     /old_locus_tag="ELO34_29085"
     CDS             complement(join(5416..5596,1..365))
                     /locus_tag="ELO34_RS29305"
                     /old_locus_tag="ELO34_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(391..717)
                     /locus_tag="ELO34_RS29310"
                     /old_locus_tag="ELO34_29090"
     CDS             complement(391..717)
                     /locus_tag="ELO34_RS29310"
                     /old_locus_tag="ELO34_29090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1075..1233)
                     /locus_tag="ELO34_RS30045"
     CDS             complement(1075..1233)
                     /locus_tag="ELO34_RS30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1873..2049
                     /locus_tag="ELO34_RS29315"
     CDS             1873..2049
                     /locus_tag="ELO34_RS29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2039..2383
                     /locus_tag="ELO34_RS29320"
                     /old_locus_tag="ELO34_29100"
     CDS             2039..2383
                     /locus_tag="ELO34_RS29320"
                     /old_locus_tag="ELO34_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2592..>3026
                     /locus_tag="ELO34_RS29925"
                     /pseudo=""
     CDS             2592..>3026
                     /locus_tag="ELO34_RS29925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3061..3570
                     /locus_tag="ELO34_RS29330"
                     /old_locus_tag="ELO34_29110"
     CDS             3061..3570
                     /locus_tag="ELO34_RS29330"
                     /old_locus_tag="ELO34_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3577..3789
                     /locus_tag="ELO34_RS29335"
                     /old_locus_tag="ELO34_29115"
     CDS             3577..3789
                     /locus_tag="ELO34_RS29335"
                     /old_locus_tag="ELO34_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4165..4293)
                     /locus_tag="ELO34_RS29930"
     CDS             complement(4165..4293)
                     /locus_tag="ELO34_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            4456..4680
                     /locus_tag="ELO34_RS29340"
                     /old_locus_tag="ELO34_29120"
     CDS             4456..4680
                     /locus_tag="ELO34_RS29340"
                     /old_locus_tag="ELO34_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4664..4930
                     /locus_tag="ELO34_RS29345"
                     /old_locus_tag="ELO34_29125"
     CDS             4664..4930
                     /locus_tag="ELO34_RS29345"
                     /old_locus_tag="ELO34_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5000..5350)
                     /locus_tag="ELO34_RS29350"
                     /old_locus_tag="ELO34_29130"
     CDS             complement(5000..5350)
                     /locus_tag="ELO34_RS29350"
                     /old_locus_tag="ELO34_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1708..1787
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1125..1522)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2374..3905
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctcatta taaatattag tggattatca tttttcttcc cccatgggtt tctaattaat
       61 ccttcgatgt actcaggtcc tttgcgtcct attgcctgcg caatattttt tatttcatct
      121 gcgtgagggc tttgttgtac agctttgttt atgcctgctt gagtgatttt cagagactct
      181 acaccgtgta tgttgtatga gataaaatgt aataaattaa gatgcaatgc aattaatcca
      241 tttttgttta catctgagta tttggataaa aaacgtaatg aagtgttaac tctcccttga
      301 agcctaacaa ttgggagtgc tgccttgtat atttccttct ccgttaaata ccgtttaaaa
      361 aacataattc tccgcctccg tatagcgtgc ctattttatt tcgtcgtaaa acttagctct
      421 gaaatattcc tttagaacca tcatgtcatt aataattgaa tctatctcat ccattttttc
      481 ttggtattct tttaatggtg aagggagcgg taactcaata tttgaaaagt gattggcgag
      541 atagggatca aggtttctca tccattcagg cttttcgcca atgcgttctt caactgaaag
      601 tgaacctgat aatctctggt taataagatc acttagactc attttggttt tattcgaaag
      661 ccgttgtaag gattgcttta atcctatatc tagcatgata tttgttcttt ctctcattac
      721 ccccccttgc tattgcgttt tttgttgcgc acaaagctac cattgcgcat taagaaacgc
      781 aataaaaaag gagggaggga gtggtttgta catgatgtgc agtgcgctga gggcatcagt
      841 ggcgggtgtt tttccgtggg atggatggag ctccggggat aacagaactg acgatcattt
      901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct cttgcactgt aaacgaaaaa
      961 accgcctggg gaggcggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgggt
     1021 aactgggtat acgagaccgc agataccaaa actgttcttt cagtgtagcc gcagttaggc
     1081 acccacttca agacctctca atatctctct cgtatatatc cagttaccaa tggctgctgc
     1141 cagtggcgtt tttgcgtgtc cgtccgggtt ggactcaaga tgatagttac cggaaagggc
     1201 gcagcagtcg ggctgaacgg ggggttcgtg catacagccc agcttggagc gaacgaccta
     1261 aaccgagccg agataccagc agcgtgagct atgagaaagc gccacgcttc ccgaaaggga
     1321 aaaaggcgga acaggtatcc ggtaaacggc agggtcggaa caggagagcg cacgagggag
     1381 ccgccggaga gaaatcaccg gcatctttaa gtcctgtcgg gtttcgtcct cctctgactt
     1441 gagcgtccat ttctgtgatg ctcgtcaggg gaggcggagc ctatggaaaa acggcaacgc
     1501 cgcatccttt tctccggccc tttctgattt gagcgccttt gctttatccc gttatccgtg
     1561 agtcggtcac gcccgccgca gccgaacggc aggagcgcag ctaccgagtg agcgaggagg
     1621 cgtcatttca tctgtccgtg catggtattg cggacgctcc tgagccacat atcacacctg
     1681 ccacatgagg cactttcatg aatcgtgctc tcatgccaac atagtaagcc agtatacaca
     1741 gcgtaaacgc tgtgactggt tcagggctgc gccccgaaac ccgctaacac cactgacgcg
     1801 ctggcgcttg tccaacgccg gagcgacccg gaaaactttt ccgtgccgtc cggcgtttgc
     1861 gaaggaggga taatgcagaa gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg
     1921 catccggcag tgatggcaaa tctgatgcgt caggcacagg aggaagatta ccgccgggag
     1981 cagatggcag agcttgagcg ttcagcccgg tcacattttg cggatatcgg aggagaggat
     2041 ggcggttaag cgtacccgtt ttttaggtat ccgggtgacc gatggggagt accagcagct
     2101 gctggagcgc tgcaatggaa ggcaactggc ggtgtggatg cgggaaacct gtctcgatac
     2161 gcgcccggcg cggtcgttgc ggctgccgtc catcgatccg gttttgctgc gccagcttgc
     2221 cgggatgggg aacaacctca accagatcgc ccggaaaatt aacggcggcc agtggtcagg
     2281 cgctgaccgg gttcaggtgg tggccgcgct gatggccatt gatgccgaac tcgagcggct
     2341 gcggcatgcc gtgctggaaa agggggcaga cgatgatcgt taagtttcat ccgcgagggc
     2401 gaggcggcgg tgccgggccg gtggattatc tgctggggaa agaccgccag cgcgacgggg
     2461 ccatcgtcct gcagggaaag ccggaggagg tccgggagct tatcgacgcc tcgccctacg
     2521 ccaaaaagta cacctccggc gtcctgtctt ttgctgaaca ggatttaccg cccggccagc
     2581 gtgaaaagct gatggcgagc ttcgagcggg ttctgatgcc cggactcgat aaagaccagt
     2641 acagcgtgct gtgggttgaa caccgggaca aggggcggct ggaactgaat tttctggtac
     2701 cgaacacgga actgctgacc ggcaggcgtc tccagccgta ctacgaccgg gcagaccgtc
     2761 cgcgcatcga tgcgtggcag accgtggtga acgggcgact ggggctgcac gacccgaacg
     2821 caccggagaa ccggcgcgcg ctggtgacgc catcttcgtt acccaaaacg aagcaggaag
     2881 ccgcagaggc tattacgcgg ggcttactgg ccctcgcctc gtcaggggag cttaaaacgc
     2941 gtcaggacgt cactgaggcg ctggaaagcg caggttttga ggtcgtgcgc accacaaaga
     3001 gcagcatcag cattgccgac ccggacggag ggcgaaacat ccgacttaag ggagccatct
     3061 atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag
     3121 agtaccggcg aaatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa
     3181 ctgagcgaaa gcgggaagag aatcagcgcc gccatccgcg cccacgagca gggtatgagc
     3241 gcggccatgc agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac atggctgacc
     3301 atcacgatgg tgtcaggact gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg
     3361 agcttgatag cgtcaaatct ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg
     3421 aacgcgaaga cctggggcgt gacgtacctg gaagacagca acggacgttt tctggtgctg
     3481 ccgaagggga cggccgtaga caggacgcag agctggacgg tcgggaacgg gcggtcgaag
     3541 cagaacgcgc tgagactggt gaaggagtaa gtgccgatga ccgagctgga aaaacagttg
     3601 ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc
     3661 gccttcgggg aatggcggaa aatgtcaggg cttatgcaac gggagaacgc ggcgctgagc
     3721 gagcgcgtga cgcgcttgag tcagcaggtg gaacgcttga gcgggcaact gcagcgtttg
     3781 agccggtagt gcagcgtcat gagatggccg tggccgcaga acgggcgcac gagcaacggc
     3841 agcacgaaaa ggaactgacg gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc
     3901 tgggctgaaa tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa ccggatgtca
     3961 ttcacctggc tgcatggcta tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt
     4021 tatcgggtgg tttgttgccg gttttacctg actgccaccc cgttcgcggc gaacccgtcc
     4081 ggggcggtgc gggcaacggg tgttatgttt aatcagcgtg aacggcagat acaaaaaagc
     4141 cccggagggc ttttaactac tgacttattt ttttgaagat ttgctgggtt tcaatttgtc
     4201 agtatttccc ggcgtttttt tattgtcacg ctggcgttta tcaggttccc ctgttgaatc
     4261 ggcaattcgt ttcggacccg gcttaagacc cataatcgtt atcctttcaa tagttaagag
     4321 gaaagccctc aagctagata tattgaccgc gttacggatt atcaagaggg gctaacaaga
     4381 ttattttttg ttcttttagg ttgattgtgg tactgttgat atacatgtat agcaaatggt
     4441 gttcgggagg tttatatgct tgctatccgg ttatctgatg agattgagtc ccgtctggac
     4501 tcgctggcga agcaaaccgg cagaacaaag acgttttatg cgcgggaagc aatactggcg
     4561 catctggaag acctggagga ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt
     4621 ggtgatgaag cagtgcattc gtctgaagac gtgaggaagt cacttggtct ggacgattaa
     4681 ctattccgat cgggcgctca aatcgttacg caagatggac aaacagaacg cacgacggat
     4741 tgtggatttt atgagtttac gcattgcagt tgctgccgat cctcgccagt cagggaagcc
     4801 gctcaaaggt gagctgggcg agttctggcg ctatcgggtg ggagattatc gcgttctgtg
     4861 tgagatccga gatgacgagc ttgttatcct tgccgccacg attggacatc gccgcgaagt
     4921 ttacgactga agcccgctat gcgggctttt ttgatgccga caacaggtat tatggtaaat
     4981 actcgactaa ctacgtgttc tactgtttgt cggttttagt taaccagcag gttgtcttaa
     5041 cgataatgta gccgttattt ttaactaata aggtcatatt gttaacgtca ggcataataa
     5101 aagacattat ggcctcagag ttatcatcag aagcagctct ctgcattact acttcgattc
     5161 ccttttcttt gaatctataa gtatccagtt gcctattaaa cttagaaaga cctacaacca
     5221 tacttagttt gttatcttta acagtaagag atgcttcctg agtaccatta catcggtagt
     5281 aagcggtgta accgtcagca aaagatcgac cggggaatat aaatgctagg aacgcaatta
     5341 accaatacat agttgagaat cttggtatta cacatttcac tatcctctct ttcatttttc
     5401 gttcaccata ctttgttatc tgatttttaa tttttctatt ttataaacca agtctctacc
     5461 aaaaaggcga aaattatcct caaataataa taattgttta agaacatcca tgaaagcagc
     5521 tgcttcaccc tcagttatgg gagctactct atataacata taacctgagt taagggtttc
     5581 ttttatactt tcttca
//
