LOCUS       NZ_CP045265            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 16HN-263 plasmid p16HN-263_2, complete
            sequence.
ACCESSION   NZ_CP045265
VERSION     NZ_CP045265.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10353651
            Assembly: GCF_011066505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yang,X.
  TITLE     Complete sequence of Klebsiella pneumoniae strain 16HN-263
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2019) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Kowloon Tong, Hong Kong
            999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:23:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,763
            CDSs (total)                      :: 5,641
            Genes (coding)                    :: 5,476
            CDSs (with protein)               :: 5,476
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 71 of 165
            Pseudo Genes (incomplete)         :: 104 of 165
            Pseudo Genes (internal stop)      :: 16 of 165
            Pseudo Genes (multiple problems)  :: 25 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045265.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-263"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-263_2"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            311..556
                     /locus_tag="ELO34_RS29240"
                     /old_locus_tag="ELO34_29015"
     CDS             311..556
                     /locus_tag="ELO34_RS29240"
                     /old_locus_tag="ELO34_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            830..1201
                     /gene="tolA"
                     /locus_tag="ELO34_RS29245"
                     /old_locus_tag="ELO34_29020"
     CDS             830..1201
                     /gene="tolA"
                     /locus_tag="ELO34_RS29245"
                     /old_locus_tag="ELO34_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1198..1563
                     /locus_tag="ELO34_RS29855"
     CDS             1198..1563
                     /locus_tag="ELO34_RS29855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1738..2016
                     /locus_tag="ELO34_RS29255"
                     /old_locus_tag="ELO34_29030"
     CDS             1738..2016
                     /locus_tag="ELO34_RS29255"
                     /old_locus_tag="ELO34_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2004..2318
                     /locus_tag="ELO34_RS29260"
                     /old_locus_tag="ELO34_29035"
     CDS             2004..2318
                     /locus_tag="ELO34_RS29260"
                     /old_locus_tag="ELO34_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2482..2910
                     /locus_tag="ELO34_RS29265"
                     /old_locus_tag="ELO34_29040"
     CDS             2482..2910
                     /locus_tag="ELO34_RS29265"
                     /old_locus_tag="ELO34_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2936..3115
                     /locus_tag="ELO34_RS29270"
                     /old_locus_tag="ELO34_29045"
     CDS             2936..3115
                     /locus_tag="ELO34_RS29270"
                     /old_locus_tag="ELO34_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3142..3672)
                     /locus_tag="ELO34_RS29275"
                     /old_locus_tag="ELO34_29050"
     CDS             complement(3142..3672)
                     /locus_tag="ELO34_RS29275"
                     /old_locus_tag="ELO34_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3679..4410)
                     /gene="mobC"
                     /locus_tag="ELO34_RS29280"
                     /old_locus_tag="ELO34_29055"
     CDS             complement(3679..4410)
                     /gene="mobC"
                     /locus_tag="ELO34_RS29280"
                     /old_locus_tag="ELO34_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4410..6374)
                     /locus_tag="ELO34_RS29285"
                     /old_locus_tag="ELO34_29060"
     CDS             complement(4410..6374)
                     /locus_tag="ELO34_RS29285"
                     /old_locus_tag="ELO34_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7858..8007)
                     /locus_tag="ELO34_RS29290"
                     /old_locus_tag="ELO34_29070"
     CDS             complement(7858..8007)
                     /locus_tag="ELO34_RS29290"
                     /old_locus_tag="ELO34_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8092..8349)
                     /locus_tag="ELO34_RS29295"
                     /old_locus_tag="ELO34_29075"
     CDS             complement(8092..8349)
                     /locus_tag="ELO34_RS29295"
                     /old_locus_tag="ELO34_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8359..10044)
                     /locus_tag="ELO34_RS29300"
                     /old_locus_tag="ELO34_29080"
     CDS             complement(8359..10044)
                     /locus_tag="ELO34_RS29300"
                     /old_locus_tag="ELO34_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3359..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7177..7755
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3683..4410)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 acatttaaaa caataaatta aagccaacat actgtacata tacacagtat aattattacc
       61 ttactcctcc cgttttcagg tgtcaatata tgagattagt tctcatcttc aggcacaaaa
      121 aagcgcccgg agccacctgt aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac
      181 aaattttatt atttaagaca ttcatcacac ttacatttca gatggtatag acacatctga
      241 aagctaactg gcatgaggag aggacctgcg gttaaactgt tcgggaaacc tgagacgccc
      301 ggaggccact atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc
      361 ctttgtcgct caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt
      421 aaaacaacag gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg
      481 ggcgcaggaa atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc
      541 acagcgccgc cggtaagggg tatttaccat aacgtcgcat tgcccgcacc gctcagacgg
      601 tctgagggcg tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc
      661 cggaaacgcg cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg
      721 gccgtttggt gtaaaaacga tacgataagc cgtgtttatg taaatgaacg tggccgtaaa
      781 gagtgacaaa tatacatgta acatttttta ctgacaggtc atcaacccga tgcgtttaat
      841 accctttatc ctgctggcat tgtgcagccc cttactcctg acggcctgta agcattccac
      901 cacccagccg ccgaaaattt atactctgga taagtcatct aaaatcagta gctacgctgc
      961 ggccatcaag accgctgttg aaatgcagat gtttgaacca gaggcctatg cgggaaaaat
     1021 gtgcaccgtg cggatatcca tacaacgtga tggcactctg aacagcgcca ttgctgaagg
     1081 aggagatccg aaactctgta aagcagcaat atcagccgtt acccacgcca aaattccgcc
     1141 ggcaccggat gaaaatacct ggcaaatatt caaaaacgcc cctctggatt tcagaccatg
     1201 aaagctttta ctctgacagt gctggcggcc atgttcctca ctccggtaca tgcgatacca
     1261 ctaaaacccg taaccacact gcagccaacc tacccggtac aggccgcagc aaaacgcatc
     1321 gaaggtcagg tgaccgccac gtttgatatt ctggacgatg gacgagtgga caacgtgtcg
     1381 cttaccggct gtcacctttt ttttcgggaa acgctgacgg caatgaggca ctggctatat
     1441 cccgcagggc acccggcaat gcgacaggtt ataaattttg aattcagtct gaaaaatgac
     1501 ggcttacagg atccgcgacc agtcaccacc caaaagctca atgatgatgg ttgccgccag
     1561 taacaggtcc tttaacccgc aggctgcaat agtcctggat gatgagtgag cctgtagcag
     1621 ccctgattag tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt ctggcaaata
     1681 gcgccgccac ggattaaagt agtcatgtgt aatacatatg actacaaagg ggcttttatg
     1741 tcatcagcaa catccatcag gctcgatgaa gaacttaagg accggctgaa aacgctggcc
     1801 gatgaccggc accgctcagc gcacgcgcta atgctggagg caatcaccga atacatcgac
     1861 cgggaagaga agcgcagcca gtacctgcgg gacggacagg ccgcatggca gcactatcag
     1921 gaaacgggtc tgcaccttac tgcggaagaa gcggaagcct ggataagtac ctggggaact
     1981 gagaacgagc aggatgctcc gccatgccac aggtaacgat ttccgcgctg gcacagcgcg
     2041 atttacaacg ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag
     2101 gtgaggtgat cgtccgggct atccagcaac tgaaaacatt gccagacatt ggccgcccgg
     2161 tgccgtttct gccgctggaa tataaggaac tggtgatcgg gtttggcgac agtggctatg
     2221 tgatgctcta ccgccacgac cgggaaatgg accagattgt gatcgtcacc gtcaggcatc
     2281 agaaagaatc cgggtatccg ggggcagaca gcctctaata accgatgacg ggggcgcaat
     2341 tcattgcgtg gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca catcctgaac
     2401 ggaaaaacgg gtaaaattca gcgatacatt taccgtgaga aattacacac ctttcacacc
     2461 cgataaaatc aggggtatac agtggcgaaa acattgctca acctttcaca ggcagcacaa
     2521 gccgcgggca ttaccagaag aacgctatac aatcatgtaa aacagggaaa ggttaccgtg
     2581 tcacgtgacg ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga
     2641 aatgtgaata tccctgagaa acaaattccc ggcatttcac accgtgaaaa tacacaaaaa
     2701 aatttcccac aggaacagct tctggcaatg caaaaggaac tggccgatct ccggcaggct
     2761 gtcaccctga tgctggaaga taaaacatcc cgggaagaag aacgccgcca gcacgatgat
     2821 gagcgccgga aactccaggc cgaagtggac agattgacaa cagaactgac acagaagaaa
     2881 aaacgcttct ggtccggctg gtttagctga gaacttaccg aagggaatgg agaacatgac
     2941 gaagcaggaa aaaactgccc tcaatatggc taaattcatt aaggatcagt cgctgctgtt
     3001 gctggaaaag ctgaacgagc tggacatgga taccccggcc gacatgtgcg aacggcttca
     3061 cgaggatgcg gaaacactct ggcaggtgat gcgggataag ctcggggaag aataacagca
     3121 ccggccatgc cggtgctggc gttatttttt gccgaacggc caccaccggc gggcgggttt
     3181 gtcctccggc agttcaggca ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag
     3241 ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat
     3301 catcgcgtta atctgctccg gtgagcactt cccggcatca accacctgca caccgttctc
     3361 gatggcgaac cgctccacct ttcgatagag gtaatcacac agcttcattc ctgtgggttt
     3421 gtcccgttcc cattgcggat tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt
     3481 gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg cccggtcgac
     3541 aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatgtctt ccagatgctc
     3601 gctggttggc ataatgcagc gctcgccgtc tttgtcttcc acgtcctcaa taaataccat
     3661 gtacagcgcc ataatccctc actccgttgt cataaattta aagcacgcga acagcctctc
     3721 ccggtccagc tccaccgttt tgcgggcctc gccggtctgt ggattcacga tttcatccac
     3781 cgccagaatc gcccgctgta cccggacaag cgtcggccgg tcagggcaga tgtaccagat
     3841 ttcatcgatg ccgttcgccc ggcggttgaa cagatacccg gacaccactt cctgataccg
     3901 cctgcggctc ttgacggtgc gctctacctc aaatgccacc gttctgccgt ccagctccgc
     3961 cagcgcgtcc ggcactttca tccctttcag ggccatacgg tgcaggtacc gccagccggt
     4021 ggccccccgg ccctccatgg agaggcgcag ggactgcacg gccagccggt gcggcagctg
     4081 cgccgccgac acccttgacg gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc
     4141 gaacgagaac gccaccccgt gcggcgtcag cccccacacg ctgacctgac ggccgtcaac
     4201 cgggtatttc gcccggacca gcagcccttt cgccaccaga ctgtccagga agcggtaaac
     4261 cggctgtacc gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc
     4321 ttcccgcagc cagcgcaggg tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc
     4381 gtggctgacg ttgtactgct ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct
     4441 tttggtccgc cgccgtcggc cggaggcagg tactccgcca gcgggtccgt tttcgccagc
     4501 accgggaagg ttttcagcga aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc
     4561 agccgcgcca cgcccagccc ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg
     4621 ttcacgtcaa acagcggccg cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc
     4681 acgttccccg cctcccggct ggcccggcgg cgctccacgt ccacccggat ctccccggtc
     4741 tggcccgccg cccactgcgc ggactcctgg ctggccgcgc gatagaacca gcggatcggc
     4801 gtgttatcca gtaccgtggt tttcacaaaa tcagcgggca gatcctgccc gcactgaccg
     4861 aagtcgccca gcgactggtg cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc
     4921 agcgcgttca gcacgtactt cgacagcaga tatttaatct catcgagcat gatgctcgcg
     4981 tgcggccacc tccggaactc atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc
     5041 atcttctgca cccggatcac cgcctcatcg tccatcgagc cgatgacgta caggcagccg
     5101 ccgccgttga ttatccccgc cacatcgata ccgctgtccg tctgcaggac gctcaggtcg
     5161 gccacgttct ccagcttggt gatcagcccc ttcacgtcct ttttcagggc ctccggcaac
     5221 gcgtacgccg ccgccagcac ctgctgaatg tttgccccct gcgggaactg ctccgcaatc
     5281 agcttcgccg ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc
     5341 aggttaaacc cggcaaccag cagattcttc agggcgtact ggtcgagatc gcggaacaga
     5401 ttcagctgcg ccggtttccc cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc
     5461 cggaagacgt gcggcgccca cgcgtcccct ttggggtcga agtacaccac agcgtccccg
     5521 aaatcgcgta cgcagcgaat gagcgcgttg gtggccatcg cgctcttgcc gctcccgggc
     5581 aggccgagga tctgaatatt ggtcttgcgc caggtatcac gagggaccag caccggctgg
     5641 ccctgctcat ccttacccag aaacaccgcc tgctgtttcc gggcctgctc cgtggaaaaa
     5701 tcggccagcg cgttaaccgg taccggctcc ccgaaatgca ggtggcggac gtctgtcatt
     5761 tctccggtcc gctggctgct gcgccggctc tcctgattca gtttcgcgtt caccgcctcg
     5821 tcagcggccg caaaatgacc atcaagcagc aggaacgcca gcagccgcgc cagaaggacg
     5881 ccggcgacaa gccctccggc acaccaggct atcccgaaca ggttagccgg ctgcagcagg
     5941 taataatctg cgttcgcata gaggtccggg cgcaggacgg tttctcccca gctctcgata
     6001 aacacggcga caaccagccc ccccagaagg gccagaccgg cagcgccggt cagccagtac
     6061 gcgtagttcc agacatgtcg ccgtgccctg accatccgcc cgggtgaatc cagcgaggcc
     6121 agcaccgaaa atggcacagt ccggttatta tcgcgcttat agcgatcggc atactcccgc
     6181 cgcgccttat ggctcagccc cacaaaacgc attcggccgg cccagagttc agccagaccg
     6241 aaaaacaggc cgataaccag aaaaaaaccg gccagactgt aatactcccg gtacagccag
     6301 ctgacccacg ggtaaatctg ccgggccagc tccgggatat gccactgcca ggcaagcgaa
     6361 tccgtattaa acatgcaatc tctctccttc agacgacggg tttaccggcg tgatgcgcag
     6421 cccccgctgc acaagccgcg gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg
     6481 tgattgcgtt cagctttcac cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg
     6541 cagccccgga ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt
     6601 gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc
     6661 cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt
     6721 gggtggtcag gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag
     6781 ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag
     6841 caaggtcttt tcccctgaag tcagccacgc cccagcgggg atgacttgca tttctccgca
     6901 aggagaaaaa agcaacagag ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt
     6961 tcacctatcc tgataattga tcgtcaggga atagaaagac ttaatcaggg gacaatatcc
     7021 cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca
     7081 ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta
     7141 cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacggc agggccgcgc
     7201 cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt
     7261 tcggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc
     7321 gacaggactt aaagatcccc accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt
     7381 ccgaccctgc cgtttaccgg atacctgtcc gccgttctcc cttgcgggaa gtgtggcgct
     7441 ttctcatagc tcacacactg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc
     7501 tgtaagcaag aactccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt
     7561 gagtccaacc cgggaaagca cgcaaaacgc cactggcagc agccattggt aactgggagt
     7621 tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg
     7681 ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag
     7741 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
     7801 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7861 ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc cgccctgaac
     7921 atcccggata taatttgcct gacatgcggc cagaagaaag ccagcaacaa tcaatataaa
     7981 aagaaaaata gtctttacct ttttcatata ctttccctgt atttaaagac tccggcattc
     8041 atccctgaac accggaagtc agtatttcaa atcatccttg ataatttttt gttaccagtt
     8101 accgtcccga taatcaaaag ctatataaca attatccaca tcaggtttta tttgattttt
     8161 aaaaaatggc tgtagtaaag gaacccattc tttcttcaaa tcaaactcac cattattaat
     8221 attatccttt aaaggcattc ccaagctttc aatgacagaa ccatcgtcac caaagtcttt
     8281 agaatactct tcccctttaa actcttcagt tttcttatca aaccatgtca aacgtaattt
     8341 aagccccata tttacctctc aaagatattt tttaatgttc cgttttggat ctggcccttt
     8401 aacctgctta cccgttttgg gatcaaatgc gcctaggtgt tcgccatcac tggcacgata
     8461 cccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgttttt tatcgccata
     8521 ccatcgggcc cgcttaccac caccaccttg tttaggtgtt ttaggatcac ctcgctttaa
     8581 ctctccaagt ccctttatgt cttctgtttt aggtactgga tgataatcat ggccgtagtc
     8641 tttagtcccc ttgcgaggct tgttcttttc ctcatttaac ttattttctg catccttcgc
     8701 ttttttctcc ttttgtttcc ggctctccat cgccccggta agtgcagcct tatctctgga
     8761 taaggattca ttcattgcgt ttaaagatgc ctgagccgct tttaatttct cattctgatt
     8821 ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg
     8881 cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc
     8941 ttcctgaaca gcagcccttg caccggggag cgccttctct gtcgatgcaa tgcggctgtt
     9001 taaggtcgca atatttttat cttcggcatc cagctcagct ttcgctttat catagtctct
     9061 tttcagcccc tcttccggat gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc
     9121 ctgacgctgt tttacctgtg ccggggtaag aacatctgtc accgacacat aaaccggctt
     9181 ctgtccggtc tctttcggga aacgaataac ggcctcacgg gagttaccac cggctgtaaa
     9241 accagccgga cgtgaatcac ctttttcagc aacaatgcct tttggcgggg ctttcgctgt
     9301 cggaacacct ttcggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa
     9361 tacccccgga cgttttgtcg gtttcgcatc caccacaggg acactcattg gccgacctgc
     9421 gacaaccgca atatgctgcc gctcatcctt caccacatcc acaacccgtt gtctgacacg
     9481 aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc tccgtcaccg catcggccgg
     9541 taatgacgtc ataatttttg acatcatttt cggatcatct tttgcaatct cagaaggcag
     9601 cacaccgtag atcgcaatcc cccacagacc aaacttaaac ggccccttca gggcagccag
     9661 cacatcagca acggcggccg acaacgcatc gccggaaact gataaagcca gtccttcagc
     9721 ccccggagta gccagggcag gcagaccgaa ggccatcgca gtggccgaag actgtccgcc
     9781 accagagcta ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc
     9841 gttaccaaac tcccccggat tatagttatg aatatctcca ttcggcgtat gcgtcgtacc
     9901 ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac ctttaccaga
     9961 cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg aattacccgg
    10021 acctcgaccg tctccaccac tcataccgtt ttcctctttg
//
