LOCUS       NZ_CP045212             9058 bp    DNA     circular CON 03-JAN-2024
DEFINITION  Escherichia coli strain 4 plasmid pCol-4, complete sequence.
ACCESSION   NZ_CP045212
VERSION     NZ_CP045212.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13045724
            Assembly: GCF_020809365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Allue-Guardia,A., Koenig,S.K., Bosilevac,J.M., Feng,P. and
            Eppinger,M.
  TITLE     Enterohemorrhagic Escherichia coli O113:H21 strains are
            differentiated by distinct genome and virulence traits indicative of
            their Stx-conferred pathogenic potential
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Allue-Guardia,A., Koenig,S.K., Bosilevac,J.M., Feng,P. and
            Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2019) Biology, University of Texas at San Antonio,
            One UTSA Circle, San Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2018
            Assembly Method                   :: SPAdes v. v3.5
            Assembly Name                     :: 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35x
            Sequencing Technology :: Oxford Nanopore MinION, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/03/2024 08:07:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,047
            CDSs (total)                      :: 4,925
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 80 of 191
            Pseudo Genes (incomplete)         :: 105 of 191
            Pseudo Genes (internal stop)      :: 46 of 191
            Pseudo Genes (multiple problems)  :: 37 of 191
            Pseudo Genes (short protein)      :: 2 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045212.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4"
                     /serotype="O113:H21"
                     /isolation_source="Ground beef"
                     /db_xref="taxon:562"
                     /plasmid="pCol-4"
                     /geo_loc_name="USA"
                     /collection_date="2005"
     gene            complement(1006..1197)
                     /locus_tag="GC226_RS25025"
                     /old_locus_tag="GC226_25440"
     CDS             complement(1006..1197)
                     /locus_tag="GC226_RS25025"
                     /old_locus_tag="GC226_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1263..1586
                     /locus_tag="GC226_RS25030"
                     /old_locus_tag="GC226_25445"
     CDS             1263..1586
                     /locus_tag="GC226_RS25030"
                     /old_locus_tag="GC226_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_228400234.1"
                     /translation="MLTIRVTYDEHARLLERCEGKQLAVWMRRVCLGEPVARFGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVRGQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1795..>2226
                     /locus_tag="GC226_RS25145"
                     /old_locus_tag="GC226_25450"
                     /pseudo=""
     CDS             1795..>2226
                     /locus_tag="GC226_RS25145"
                     /old_locus_tag="GC226_25450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2264..2782
                     /locus_tag="GC226_RS25040"
                     /old_locus_tag="GC226_25455"
     CDS             2264..2782
                     /locus_tag="GC226_RS25040"
                     /old_locus_tag="GC226_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_143369636.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGSSGMRLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQDGRRKFLVLPYGKSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            2789..3022
                     /locus_tag="GC226_RS25045"
                     /old_locus_tag="GC226_25460"
     CDS             2789..3022
                     /locus_tag="GC226_RS25045"
                     /old_locus_tag="GC226_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_105282911.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3404..4237
                     /locus_tag="GC226_RS25050"
                     /old_locus_tag="GC226_25465"
     CDS             3404..4237
                     /locus_tag="GC226_RS25050"
                     /old_locus_tag="GC226_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme EcoRI"
                     /protein_id="WP_001565219.1"
                     /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKA
                     LSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEW
                     RVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKF
                     VNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK"
                     /name="type-2 restriction enzyme EcoRI"
     gene            4267..5247
                     /locus_tag="GC226_RS25055"
                     /old_locus_tag="GC226_25470"
     CDS             4267..5247
                     /locus_tag="GC226_RS25055"
                     /old_locus_tag="GC226_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="WP_002670781.1"
                     /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVVY
                     CNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKENGK
                     KLVFDDISVSSFCGDGDFRSSESIDLLKKSDIVVTNPPFSLFREYLDQLIKYDKKFLII
                     ANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRIISPN
                     NCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGVMGVPI
                     TFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK"
                     /name="adenine-specific methyltransferase EcoRI family
                     protein"
     gene            complement(5743..6171)
                     /locus_tag="GC226_RS25060"
                     /old_locus_tag="GC226_25480"
     CDS             complement(5743..6171)
                     /locus_tag="GC226_RS25060"
                     /old_locus_tag="GC226_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_033802653.1"
                     /translation="MKRSGGTRYMYLSALITVAAALTACTPKTSLEQHTRHYVYASDGG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLRTT
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6252..6689)
                     /locus_tag="GC226_RS25065"
                     /old_locus_tag="GC226_25485"
     CDS             complement(6252..6689)
                     /locus_tag="GC226_RS25065"
                     /old_locus_tag="GC226_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_171821344.1"
                     /translation="MGLCHFMAWHTLRDAMKMTGKSRSQFYRDMRAGLVSYRTGQNGRR
                     EFETSELIRAYGELKQNETPERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            6948..8516
                     /gene="cea"
                     /locus_tag="GC226_RS25070"
                     /old_locus_tag="GC226_25490"
     CDS             6948..8516
                     /gene="cea"
                     /locus_tag="GC226_RS25070"
                     /old_locus_tag="GC226_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_047083621.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGIPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(8513..8854)
                     /locus_tag="GC226_RS25075"
                     /old_locus_tag="GC226_25495"
     CDS             complement(8513..8854)
                     /locus_tag="GC226_RS25075"
                     /old_locus_tag="GC226_25495"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_228400233.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEEYYFLVTDKMLYAIVI
                     STILCPYSKYAIEHIVFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLFCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            8902..9039
                     /locus_tag="GC226_RS25080"
                     /old_locus_tag="GC226_25500"
     CDS             8902..9039
                     /locus_tag="GC226_RS25080"
                     /old_locus_tag="GC226_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072022881.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSELVGITVQ
                     "
                     /name="colicin release lysis protein"
     Typing          834..915
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(265..814)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.315"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1577..3120
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.951"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 aacggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcga
      601 cgcggccttt ttacggttcc tggtcttttg ctcacatgtt ctttcctgcg ttatcccctg
      661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
      781 tccttatgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      841 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
      901 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
      961 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     1021 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     1081 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agtttctcca gcagcgttaa
     1141 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tagtcactga
     1201 tgcctccgtg taagggggga tttctgttca tgggggtaat gataccgatg aaacgcgaga
     1261 ggatgctcac catacgggtt acttatgatg aacatgcccg gttactggaa cgctgtgagg
     1321 gtaaacaact ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgtttcg
     1381 ggaagctgcc gacactggca ccgccgttac tgcgtcagct ggccgccatc gggaataacc
     1441 tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggcgac cgggttcagg
     1501 tggtggccgc actgatggcc atcgagagag agttgcgcag cttacggcag gtcgtcaggg
     1561 gacagggagc gcgggatgat agttaagttt catgccaggg ggaaaggtgg tggcagtggt
     1621 ccggttgatt accttctggg aagggagcgt aaccgggaag gtgcaacggt actgcagggt
     1681 aacccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa atatacgtcc
     1741 ggtgttctgt cgttcgcgga gaaggaactg ccgccgggag gacgtgaaaa agttatggcg
     1801 agctttgagc gtgtactgat gcccggtctt gaaaaaaatc agtacagcat tctgtgggtg
     1861 gagcaccagg acaaggggcg gcttgagctg aattttgtca ttccgaacat ggagctacag
     1921 accggaaaac gcctccagcc gtactacgac cgcgcagaca ggccgagaat tgatgcctgg
     1981 cagacgctgg tgaatcacca ttacgggctg catgacccca acgccccgga gaaccgccgg
     2041 atactgactc tccctgataa cctgccggaa acgaaacagg cgcttgctga gggcgttact
     2101 cgcgggatag acgcacttta ccatgccgga gagataaaag gccgtcagga tgtgattcag
     2161 gcgctcacag aggcggggct tgacgtggtc agggtgacgc gaagcagtat cagcattgca
     2221 gacccgaacg gcgggaggaa tatcagactg aaaggagcgt tttatgagca atcttttaca
     2281 gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag agaaaatgct
     2341 gaacgacgag ttcaggaagc tcggcgaatc tgtcagcgag gctgtgacct caaacgagac
     2401 gaaaatcaaa ggcgctatag cccagtttac ggcctcgacg gaggaatcgc tgaaaaaaca
     2461 ccgggaaggg gtgaaagagg cgatgatgca gcaccggaag gacatgttaa agctggcagg
     2521 gagttcgggc atgaggttgc tggggatagt ctttctcctg tttaccgcga gtggcgggac
     2581 gctctggtat ctcggcggga ggatacagac gaacctggaa gaaatcaggg tacaggaaga
     2641 gacattgcgg aaactgaacg cgaagacatg gggcgtggag tttgttcagg acgggagaag
     2701 gaaatttctg gtccttccgt acgggaaatc agcagaggtg actcactatc aggggaagga
     2761 ctggatacaa ctgacggagt gatacggaat gacagagctg gaaacacatt tgctgaacgc
     2821 cttagagcag ctgcaacagg actatatgca gcggctgaac gaatgggaga gcgccttcgt
     2881 ggaattgcag aagatgtttt cgcttacgca acgggacaac gcgatgctga acgagcgggt
     2941 catgcagttg agtcagcagg tgcaacactt gagcgagcgg acagaacgct tgagccagtt
     3001 atacagcgag aacaggagat aagggatgaa cggctgatgc aggagcgcga acatgcatta
     3061 tccctggagc gggaacgcca gctggaaata caggaacgca cactggatgg tccttcgttg
     3121 tcgctgtgat ggcgaaacta tgaaaaatgg caggttcggt ggattttgac gggctaatgt
     3181 ggtctgcacc atctggttgc ataggtattc atacggttaa aatttatcag gcgcgatcgc
     3241 ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgatgaa
     3301 cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tcaaacgtat gttaatctat
     3361 cgacatatgt aactttataa aataacagtg gaaacatgga ttcatgtcta ataaaaaaca
     3421 gtcaaatagg ctaactgaac aacataagtt atctcaaggt gtaattggga tttttgggga
     3481 ttatgcaaaa gctcatgatc tcgctgttgg tgaggtttca aaattagtaa agaaagctct
     3541 tagcaacgaa taccctcaat tatcatttcg atatagagat agtataaaga aaacagaaat
     3601 aaatgaagct ttaaaaaaaa ttgaccctga tcttggcggt actttatttg tttcaaattc
     3661 cagcatcaaa cctgatggtg gaattgtaga ggtcaaagat gattatggtg aatggagagt
     3721 tgtacttgtt gctgaagcca aacaccaagg taaagatatt ataaatataa ggaatggttt
     3781 attagttggg aaaagaggag atcaagattt aatggctgct ggtaatgcta tcgaaagatc
     3841 tcataagaat atatcagaga tagcgaattt tatgctctct gagagccact ttccttacgt
     3901 ccttttctta gaggggtcta actttttaac agaaaatatc tcaataacaa gaccagatgg
     3961 aagggttgtt aatcttgagt ataattctgg tatattaaat aggttagatc gactaactgc
     4021 agctaattat ggaatgccta taaatagtaa tctatgtatt aacaaatttg taaatcataa
     4081 agacaaaagc attatgctac aagcagcatc tatatatact caaggagatg ggagggagtg
     4141 ggattcgaaa atcatgtttg aaataatgtt tgatatatca acgacttcgc tcagagtgtt
     4201 ggggcgtgac ttgtttgaac agcttacatc taagtgatat tttttatttt aataaggttt
     4261 taattaatgg ctagaaatgc aacaaacaag ttactgcaca aagctaaaaa atcgaaaagc
     4321 gacgaatttt acactcagta ttgtgatatt gagaacgaac tgcaatacta cagagagcac
     4381 ttctctgata aggttgttta ttgcaattgt gatgatccta gagtaagcaa tttctttaaa
     4441 tattttgcag tgaattttga taatcttggc ttgaaaaagt taatagcatc ttgctatgta
     4501 gagaataaag aaggtttttc tagtagcgaa gccgcgaaga acggatttta ctatgaatat
     4561 cataaagaaa atggaaagaa attagttttt gatgatatta gtgtttcttc tttctgtggc
     4621 gatggcgatt ttcgcagttc ggagagcatt gatctgctaa aaaaatcaga tattgttgtt
     4681 acgaatcctc cattctcgtt atttagagag tatcttgatc aactaattaa gtatgataag
     4741 aaattcctta taattgctaa tgttaattca ataacatata aagaggtgtt taatctaatt
     4801 aaggaaaata agatttggct tggggttcat ctcgggagag gtgtttctgg atttattgtt
     4861 ccagagcatt atgaattata tggtactgag gcgagaattg attctaatgg taatagaatt
     4921 atctcgccaa acaactgctt atggctaact aacctagatg tctttattag gcataaagac
     4981 ttgcctctta caagaaaata ttttgggaat gaaagttcat atccaaaata tgataattat
     5041 gatgctataa atgtaaacaa aacaaaggat attccattag attacaatgg ggttatgggg
     5101 gttcctatca cattcttgca taagtttaac cctgagcaat ttgagttaat aaaatttaga
     5161 aagggtgttg atgaaaaaga tttgtctata aatggtaaat gcccttattt cagaattttg
     5221 ataaaaaaca aacgattaca aaagtaattg atgtttgtta gttttttctt gagatcatta
     5281 gcttcgtcgt aagcttcggt accagaaacg aaaaaaccac ctctccaggt ggttttttcg
     5341 aaggttcagc aaaccggcaa gtttctgaac catggtaact ggcttaagca gggcgcagat
     5401 accaaataca gtcctttctg tgtagccgta gttaggctat cacttcaata atctgtaata
     5461 gggagccgca aacggaaatt ttaccacgct aagagaattg agttgaacga gtagttacat
     5521 gcaggccaac taatttactg aacataaagg ctgtacgaaa tggcctaact gtccgacaac
     5581 cgtttggctt acttctgtac attcccttgc gcagatgtac agaattgtac gatttcggat
     5641 acaaaaactg ctattgtccc gccttctggc aatttccaaa agttgtttgg ctaaaaaata
     5701 gtcttaacat ggtttgtttt ccgaattcca agtggcctcc acttactgtc ttccctcgta
     5761 gccatcgaga taaaccgcag aaatcgtgtc agctagcagc ttcatttttt ccggtgatgg
     5821 catatcactg ttcgtgtatt ttctgcccgc aaaggtggtt gtggttctca gtgactgaag
     5881 gaattcttca gagtggaatt cttttatgcg ttgctgtgcc gtttctgcag atactccggc
     5941 tgctttgtct ttcaccccca tctccctgaa ctgctgaaag aacggaatca tcatccgtgt
     6001 ggtgtcagct ttcttggtgt agaagttagg atcaaaccca ccatctgaag cataaacata
     6061 atgccgggta tgttgttcca ggctggtttt tggggtacag gctgtgaggg ctgctgccac
     6121 agttatcagg gcgctaagat acatatagcg tgtgccgcca gaacgtttca tgcgagtctc
     6181 cttaccttaa agtatttatt ttcggtgagt tattatctga tggctatctt atcatcagtc
     6241 tctgagcgcg ttcagcgacc gaacaacctg gaccagaatc cccgtttctt ttcctgctcc
     6301 agcgcctgcc tgagctgggc tatctcattc tgtagctgtt cccgttctgc ttcctggcgc
     6361 cggcgctccg tatcctgggc ttgtttatcc tcaagcatca gcgtcaggca ttgtttcagc
     6421 tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc tgcctgtccc
     6481 acagtgtgcc tttctggtgt ctcattctgc tttaattcgc cgtatgcccg gatcagttca
     6541 ctggtttcaa actcccgtcg tccgttctga ccggtacggt aactgacaag gcctgcgcgc
     6601 atatcacggt aaaactgtga acgcgatttg cctgtcattt tcattgcgtc ccggagtgtg
     6661 tgccatgcca taaagtgaca gagtcccata ggtgtctcat ctcatggtct cagttaaaca
     6721 taccggggcc tgagaacggt aatgtttgtg ctggtttttg tggcatcggg cgggaatagc
     6781 acgtggtgtt ggtggctgtt tttttgaacg ttttcacaaa aatgaaaatc cacagggttg
     6841 acagggaaaa cgcagcggcg taatttttag ctgtacataa aaccagtgga tttatgtaca
     6901 gtatttattt ttaacttatt gttttaaaag tcaaagagga ttttataatg gaaaccgcgg
     6961 tagcgtacta taaagatggt gttccttatg atgataaggg acaggtaatt attactcttt
     7021 tgaatggtat tcctgacggg agtggctctg gcggcggagg tggaaaagga ggcagtaaaa
     7081 gtgaaagttc tgcagccatt catgcaactg ctaaatggtc tactgctcaa ttaaagaaaa
     7141 cacaggcaga gcaggctgcc cgggcaaaag ctgcagcgga agcacaggcg aaagcaaagg
     7201 caaacaggga tgcgctgact cagcgcctga aggatatcgt gaatgaggct ctccgtcaca
     7261 atgcctcacg tacgccttca gcaacagagc ttgctcatgc taataatgca gctatgcagg
     7321 cggaagcaga gcgtttgcgc cttgcgaaag cagaagaaaa agcccgtaaa gaagcggaag
     7381 cagcagaaaa ggcttttcag gaagcagaac aacgacgtaa agagattgaa cgggagaagg
     7441 ctgaaacaga acgccagttg aaactggctg aagctgaaga gaaacgactg gctgcattga
     7501 gtgaagaagc taaagctgtt gagatcgccc aaaaaaaact ttctgcagca caatctgaag
     7561 tggtgaaaat ggatggagag attaagactc tcaattctcg tttaagctcc agtatccatg
     7621 cccgtgatgc agaaatgaaa acgctcgctg gaaaacgaaa tgaactggct caggcgtccg
     7681 ctaaatataa agaactggat gaactggtca aaaaactatc accaagggcc aatgatccgc
     7741 ttcagaaccg tccttttttt gaagcaacca gacgacgggt tggggccggt aagattagag
     7801 aagaaaaaca aaaacaggta acagcatcag aaacacgtat taaccggata aatgctgata
     7861 taactcagat ccagaaggct atttctcagg tcagtaataa tcgtaatgcc ggtatcgctc
     7921 gtgttcatga agctgaagaa aatttgaaaa aagcacagaa taatctcctt aattcacaga
     7981 ttaaggatgc tgttgatgca acagttagct tttatcaaac gctgactgaa aaatatggtg
     8041 aaaaatattc gaaaatggca caggaacttg ctgataagtc taaaggtaag aaaatcggca
     8101 atgtgaatga agctctcgct gcttttgaaa aatacaagga tgttttaaat aagaaattca
     8161 gcaaagccga tcgtgatgct atttttaatg cgctggcatc ggtgaagtat gatgactggg
     8221 ctaaacattt agatcagttt gccaagtact tgaagattac ggggcatgtt tcttttggat
     8281 atgatgtggt atctgatatc ctaaaaatta aggatacagg tgactggaag ccactatttc
     8341 ttacattaga gaagaaagct gcagatgcag gggtgagtta tgttgttgct ttacttttta
     8401 gtttgcttgc tggaactaca ttaggtattt ggggtattgc tatagttaca ggaattctat
     8461 gctcctatat tgataagaat aaacttaata cgataaatga ggtattaggg atttaattat
     8521 tctttattga tataaaaagt cctagcaatc caaatgggat tgctaggacc aaacaaaata
     8581 gattatatag cataaatagg tttaattttg ctacgggggc gttatttaga ttttttcttc
     8641 tttcgaaaaa atctttcttt atgaagttaa aaactatgtg ttcaatagca tattttgaat
     8701 atggacatag aatagtgctt atcactattg catatagcat cttatctgtc acaaggaaat
     8761 aatactcttc gctgtttttt gttataaggt atatatatat aagtgtgcag tacaggccaa
     8821 ataaaatatt ttttatgtag tatcttaagc tcataaatta aacctcgtca tatattcttt
     8881 tcattttata aggatcgagt tatgaggaaa agattttttg tgggaatatt cgcgataaac
     8941 ctccttgttg gatgtcaggc taactatata cgtgatgttc agggagggac tgtggctcca
     9001 tcatcttctt ctgaattggt ggggatcacc gttcagtaga aaagatcaaa ggatcttc
//
