LOCUS       NZ_CP045134            13580 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter indicus strain TQ18 plasmid p18TQ-13k, complete
            sequence.
ACCESSION   NZ_CP045134
VERSION     NZ_CP045134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13017013
            Assembly: GCF_009914435.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter indicus
  ORGANISM  Acinetobacter indicus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   He,T. and Li,R.
  TITLE     Emergence of plasmid-mediated high-level tigecycline resistance
            genes in animals and humans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   He,T. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2019) Institute of Food Safety and Nutrition,
            Jiangsu Academy of Agricultural Sciences, Zhongling Street, Nanjing
            210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v. 3.11.1
            Assembly Name                     :: Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:02:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,874
            Genes (coding)                    :: 2,797
            CDSs (with protein)               :: 2,797
            Genes (RNA)                       :: 106
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 28 of 77
            Pseudo Genes (incomplete)         :: 51 of 77
            Pseudo Genes (internal stop)      :: 14 of 77
            Pseudo Genes (multiple problems)  :: 15 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="Acinetobacter indicus"
                     /mol_type="genomic DNA"
                     /strain="TQ18"
                     /isolation_source="cow feces"
                     /host="cow"
                     /db_xref="taxon:756892"
                     /plasmid="p18TQ-13k"
                     /geo_loc_name="China: Jiangsu"
                     /collection_date="2017-05-21"
                     /collected_by="Tao He"
     gene            complement(join(13542..13580,1..429))
                     /locus_tag="GA721_RS15085"
     CDS             complement(join(13542..13580,1..429))
                     /locus_tag="GA721_RS15085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228277949.1"
                     /translation="MCCKKPYLKLDFGSGKYVELVRLSHSSKNSKKYASNHDKSAKNYT
                     WTWRYDEPTVHLIEKKYKEIINDLISNPNKSVGIGKWQQLNADLRHYTVFKGNRHQVGR
                     LFTQAVGYHYKKGQSNLRNAEYYQPLTLSYLPRQENYAERLHAIRDFKTSF"
                     /name="hypothetical protein"
     gene            complement(519..821)
                     /locus_tag="GA721_RS15090"
     CDS             complement(519..821)
                     /locus_tag="GA721_RS15090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228277950.1"
                     /translation="MALIFYSHFSVDGENNLFFPKIDNLIKKLTDKYHLNLNVRQRSYR
                     LNTKKEPIADLIVQKRVGSPVFDFWLFITTPNTHKFNTAAKPNQLETTSIWTTSS"
                     /name="hypothetical protein"
     gene            3338..4339
                     /locus_tag="GA721_RS14790"
     CDS             3338..4339
                     /locus_tag="GA721_RS14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020928.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_160243714.1"
                     /translation="MAISLKKAPLTTNFSARHTELFPLVQPEKKYPKELAVFQNIFWKL
                     THEKPLKDDLLKLLFFFSPKVRQVENDLLSERYVFSITATEYSELTGLKKDSCYVALSR
                     AVDTLYEHSVKFYHEETKEIIRTRLISYCGYKEGTFSVSFTHYALHIMSVFNKDNPFTQ
                     LKIRSVMPLSGYALKMYPLFLQNEFRKVFEVDLINLKSALNIEPDAYSDFKDFKKKVLK
                     PSIDLINVKTELSISYKAIKKGGRKASHVEFTIGKVSPVKIKSESTKLAPEPAKKIGAI
                     TIFKALTAYNLLERFIEHGESSDDLINRIKHDIKNDKTDYWIYKLKEFGITL"
                     /name="replication initiation protein"
     gene            4530..5093
                     /locus_tag="GA721_RS14795"
     CDS             4530..5093
                     /locus_tag="GA721_RS14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_160243715.1"
                     /translation="MNTATKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGFIDFSEL
                     LRVYGEPFGSKQVKRIDTSSIQNQNTPEYIDKTVVELKNQIDFLKKQLEKAEDREAKAN
                     ARIDTLLTLIEMKSPQANMTTFTNQEQPQPNAAQVQPEPSTDDTKHDGLTTVEPKRISI
                     PEHVESGPKKRGLLGRLTKAFFED"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5283..5801)
                     /locus_tag="GA721_RS14800"
     CDS             complement(5283..5801)
                     /locus_tag="GA721_RS14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_160243721.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSAMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            6099..7231
                     /locus_tag="GA721_RS14805"
     CDS             join(6099..6351,6351..7231)
                     /locus_tag="GA721_RS14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032072965.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba14 family transposase"
                     /protein_id="WP_225530522.1"
                     /translation="MARRPRRNHSNDFKAKVALAAIKAEKTLAELSSEFDVHQNQIIDW
                     KNQLISASSQAFDQSKAPSEPPIDLKKLHAKIGEQALEIGFFRRCVEETGPLQPQKLID
                     HSLQISVSKQAKLLKVSRGCYYYRPKPVSSSDLKLMRCIDELHMQYPFAGSRMMRDLLN
                     RQGHHIGRRHTRTLMKKMGIQALYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDI
                     TYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGSPEILNTDQG
                     SQFTSDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQ
                     LSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA"
                     /name="IS3-like element ISAba14 family transposase"
     gene            7338..7739
                     /locus_tag="GA721_RS14810"
     CDS             7338..7739
                     /locus_tag="GA721_RS14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000660211.1"
                     /translation="MKEKLRWIILVVSLAFLYGVTYFIFINTSTGKPIFGGIPEILAYI
                     VPAYFITSILIEVLLNDIPNKNVKLMLGKALILLLSFLAFTVIYWLFYKNIIIANELEN
                     TFIGKNSHFIFIWLMIFFGIKFANKLYFK"
                     /name="hypothetical protein"
     gene            8279..8533
                     /locus_tag="GA721_RS14815"
     CDS             8279..8533
                     /locus_tag="GA721_RS14815"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_160243716.1"
                     /translation="MKQSKDKSLAVAYFLLVTCGFLGIHRFYLGKNISGILFIILSYSY
                     LPFTPAIIFVGIWLIIDIFLLPTLVKSTNSLKHATCDPI"
                     /name="TM2 domain-containing protein"
     gene            9142..10572
                     /locus_tag="GA721_RS14820"
     CDS             9142..10572
                     /locus_tag="GA721_RS14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397137.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_160243717.1"
                     /translation="MNLMTVEHIRQTTNAKLNPKKKSELGQFMTPSVIAEYMASLFDDY
                     KKEVKLLDCGAGIGSLTISAAKKLKNIKLADLWEIDPIMREQLEINMHAIKLNFFIYAQ
                     DFILGAVENISSDKGERYTHAIINPPYKKINSNSEHRRELRKVGIETVNLYSAFVALTI
                     QLMEKDGQIVAIIPRSFCNGPYYKPFRELILKECSIEHIHIFESRDMAFKDDDVLQENI
                     IIKLTKGKQQSDVQISQSNDQTFRDYQSKTVPFVEVIKPNDPELFIRIATNEQPQEENN
                     TFFAVSLNELGISVSTGPIVSHRMKEFLEQSPQDGAVPLFYPHHFVDKQLQYPKEHKKP
                     NAIRVTPDSQKWLLPNNGFYVIVRRFSSKEEKRRIVANVIDPNMIDTNWIGFDNCWNVF
                     HIKKQGFDYETAMGLACFLNSSLLDNYFRIFSGHTQVNATDLRNMKYPSLQNLQLLGKK
                     YNIKMDQKQIDNLLGEIT"
                     /name="N-6 DNA methylase"
     gene            10569..11513
                     /locus_tag="GA721_RS14825"
     CDS             10569..11513
                     /locus_tag="GA721_RS14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="WP_160243718.1"
                     /translation="MNNQDTKLQQAYDILVQLGMPRQQLNERTALCLLCLLDMTPEKSW
                     NQASNPLVGITPIMNWARDHYQREYAPNTRETFRRQSMHQFIEAGVCLYNPDKPDRPVN
                     SPKAVYQIEFNLLQVLQSYGTEQYETMLQNYLQQRQTLAQMYAREREMNMIPLQLTDGQ
                     TILLSAGDHSQLIKDIVIDFGARYVPGGVLVYAGDTGNKHGFFDVELLASLGVQLDNHG
                     KLPDVVIYNPEKNWLFLIEAVTSHGPVDHKRYGELNTLFQNCTAGLVFVSAFPDTKTYV
                     KYSGVIAWETEVWMADNPSHMIHFNGSRFMGPY"
                     /name="BsuBI/PstI family type II restriction endonuclease"
     gene            11546..12955
                     /locus_tag="GA721_RS14830"
     CDS             11546..12955
                     /locus_tag="GA721_RS14830"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_160243719.1"
                     /translation="MSFSNIDPKSDYEFSKILNKFVTPSEPITTPERLFGREDELKRAS
                     RALLDPGKQIFIIGERGVGKTSLANIVASFHQSSDAEPLKTSCAEDTTFRTLISDIVIK
                     ALNRSKFKEISTKQTKGISFPLLSKISANLGQEVSVKEKVILDQIDTVSDALTILQEIS
                     ELHSENPVIVVDELDRMNKDNIRPLSDIIKGISDQRINIKFIFTAVAQTYELIFGSHES
                     TRRQLEIIELPVLSWDSRMELIEKTLNNFDISIDRTTSIRISAISDGFPHYIHFILKQL
                     LWNIFETKEVVTEIDLSSYNIALNDVVKQLSPEYSLKYHRAINTRHIDCELVLWSTSIG
                     EWIINSLDEMFEAYLEIFYLLRDEQDIPLEKTKFNSMVRDLTKAQRGKILVSSHSGKRG
                     EYEFSEKIFKGYIRIQAELHSINIKGIDEKGLITPILTQWSNIPLARTGYHQSKPPQGY
                     PRKSSVFGNYM"
                     /name="ATP-binding protein"
     gene            complement(13046..13378)
                     /locus_tag="GA721_RS14835"
     CDS             complement(13046..13378)
                     /locus_tag="GA721_RS14835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160243720.1"
                     /translation="MPKPFLGSADDLAKFLDKHFRLGVKENGSSSFKFLNKREAYSVLK
                     YVARDVINEEVERLKVEDPKAVARYNPGPSKIRLLDPLKPSTTGYIHMVLKRRYGSGNY
                     CIRPIR"
                     /name="hypothetical protein"
ORIGIN
        1 ttccgcataa ttttcttgtc taggcaaata cgataacgtc aacggctgat aatattcagc
       61 gttcctcaaa ttcgattgtc ctttcttgta gtgatagccg acagcttgcg tgaacagccg
      121 cccgacctga tgacgattac ctttgaacac ggtgtaatgt cgtaaatcag catttaactg
      181 ctgccatttc ccgatgccta ctgatttatt agggttagaa atcaaatcat taataatttc
      241 tttgtatttc ttctcgatta aatggactgt tggttcgtca taccgccatg tccaagtgta
      301 gttttttgcg ctcttgtcat ggtttgaagc atattttttt gagtttttag acgaatgtga
      361 aaggcgaacc agctcaacat acttaccact cccaaaatcg agttttaaat agggtttttt
      421 gcagcacaaa cttgaacttt tcctgatctc gaaaatagtc ctggatgatg cttatttctt
      481 tttgttcatt ctcacgattc caaacaatag tttcagcctc agctactcgt tgtccagata
      541 gacgtggttt caagctgatt tggcttagct gcggtgttaa atttgtgagt attcggcgta
      601 gtaatgaaaa gccaaaaatc aaaaactggg ctgccaacac gtttttgcac aattagatcg
      661 gcaataggct cttttttagt gttcagtcgg taagatctct gtctcacatt gagatttaag
      721 tgatatttat ccgttaattt cttaatcaga ttatctattt taggaaaaaa gaggttgttt
      781 tccccatcaa cagaaaaatg agaataaaaa ataagcgcca tgcccgacct tatctacaat
      841 tttttgcgct gtataacgac tgttcgggct gtaatgaatc attttattgt taccaagcaa
      901 tatttgttca attgtcatca aacaatattc taaaaagtcc tatgtaatca agcaatattt
      961 atgaaagttt aacatcttat tttataagac tttcataaaa atttagtggc gaagccactt
     1021 ttaccaatgc caaaggcatt ggtcggactt aattgacaca atttgtcatc gtgcaatcaa
     1081 gtggttccta ggtgacgata tttacacctg tttttacctg ctatatgatg gacttattca
     1141 ttgacttgaa gcagtctgac caactgcctg accattttcg ttttagcgat tttgagcagg
     1201 caacagcctg cttaaagcca tgttttttta gatagcgcac gttccactac tagccatttt
     1261 gggatgaccg attgagcgta ctacctctga aggggggttc ggtacgatct gatgggcgct
     1321 ggagtttact ctttcgatcc aatccaagct gtgatttttc tttatgactt ttcacggggt
     1381 tgggcgacaa ggacacggga gcctataaac tgactttttt cagtcgggga agtggtcgtt
     1441 aggttagata tagcctagct aggtgtttta ttacactcga aaccctgttc tagtgattac
     1501 cgtgagaacc tgacaggacg ttaaggttcg ttaataacgg ttatgtattg cggctcatgc
     1561 acgttggcaa caacggatag gcgcagttca taagcacaac tgtaggcgtt tgaggtaaca
     1621 gcgatgttat cggctgcgga gaggaaactt ggaagcagcg gcaccactga gtgagatcat
     1681 ctcaagcaag gtgggtcaac ggatagctta agcagtatac atcgggatgc atgaacccga
     1741 gatacgtgta gatcaagagc catacgctca gattttttct gatttgccat atccccattt
     1801 ttttaaggaa ccgcgttggg gggaaactgg aagtgcaggt caggagagag agcattggcg
     1861 gtctggtcgt taagattgct agaaataact tgggaatctc ttagctgctg ctgtcatggc
     1921 actggtaggt taagagaggg agagacgatg agtagggcgt ataaggcata taacttgtta
     1981 tatgttgaag tcgtggcaaa tattgctaaa acaataggcg aactcaattg cgactggcag
     2041 tcgtaattgg taaagggagt ctaaactcta tgtgatgttt acgtggagcc gtttccgaaa
     2101 gtttgagtcg gttttatgtt gttatctaaa aaagcgaaaa actcgttaaa gatggtttgg
     2161 tcgccatcgt tgttttgctt gggtcaggat tggtctcctg acttatgcaa gactaaacaa
     2221 cagtctaatg aaagaatgtg atgtgatatt tagccacttt tagagtgcaa cagtcttgtt
     2281 aatcaccgct ctgttcaata gtgccaggtg ctatttctct ggatagtttt aaagtttaga
     2341 aaaactgatg ttagcagaca ttccaatctg tgagttttta ctctttttta gtaaagaaag
     2401 ggcgtactgt gatgattctt aggctctgcc gacctaagtt attcgataaa cagcgtggta
     2461 gttacgtcag tgcactatag ccccctcggt tttctttggc aaagaaagcg aaacgaggtg
     2521 ggtgtcttaa tattccctca tttgcgtttt aagcaccctt ttacatttga tataggattc
     2581 attggtcatc agtgaaaaaa tcgcaaatac cccctatttt tttgcatcta atagttattt
     2641 cagagcgagt gtctacgagc gaacagacac aatcaaaatt ctcgctatgc tttttccctg
     2701 attacaagct cccccttgat tctaaaactt agatacagag catcccgaag ggtgcgaaca
     2761 aacttaattt gattctagat cgagcgaagc gagcataaaa aacttatgag cgaagcgaat
     2821 tccgagttgc ttttgctttt tatggaattc cagcaggtat ttgctaaaaa ttgccccaac
     2881 gaatcgagcg agagcgagat tcaatagagt ttgagcgtag cgaaaaccaa gggcaatttt
     2941 aaaacatttc tatttttaat cttttataaa atctttaaga gcttttaaaa gctttataaa
     3001 agctttaaga gcttttaaaa gctttataaa agctttacag gggtgctaaa acccttatga
     3061 atagatgatt tcagagcttt agggggggac tgattgttag gtaggggggg actgattgtt
     3121 aggtttttta acttaaaggg ggactgattg ttaggtaggg ggggactgat tgttaggtag
     3181 gggggactga ttgttaggta ggggggactg attgttaggt agggggggac tgattgttag
     3241 gtagggtttt gagtgaattg gataaaaaga aagcgtattg catgtttaaa gggggatgtg
     3301 tatattaagt tattccccct catttctact aaaaattatg gcgatttcac taaaaaaagc
     3361 cccactaact actaattttt cagctcgtca tactgaatta tttccactag ttcagcctga
     3421 gaaaaaatac ccaaaagaac ttgctgtttt tcaaaatatc ttttggaagc ttactcacga
     3481 aaagccacta aaagatgacc ttttaaaatt actttttttc tttagtccta aagttcgcca
     3541 agttgaaaat gatctacttt ctgaaagata tgttttttca atcacagcca cagaatattc
     3601 agaattgaca ggactaaaaa aagacagctg ttacgttgct ctgagtcggg ccgtagatac
     3661 tctttatgag catagtgtta agttttacca tgaagaaacg aaggaaatta tccgtactcg
     3721 tttaatcagt tactgcggtt ataaagaggg gactttctct gtttccttta cccattatgc
     3781 acttcatatt atgagcgtat ttaataagga taaccctttc acacagctta aaatcagatc
     3841 tgtcatgcct ttaagcggat acgctttgaa aatgtatcct ctattccttc aaaatgaatt
     3901 tcgaaaagtt tttgaagtag atttaatcaa tctaaaatca gctttaaata ttgagcctga
     3961 tgcgtattct gatttcaaag actttaaaaa aaaggttctc aaaccaagta ttgatttgat
     4021 taacgttaaa acggaacttt ctataagcta taaggcaatt aagaaaggcg gacgtaaagc
     4081 aagccatgta gaatttacta ttggaaaagt aagtccagta aaaattaaat cggaatcaac
     4141 taaattagcc cctgaaccag ctaaaaaaat tggtgcaatc acaattttta aagctttgac
     4201 agcatataat ttacttgaac gcttcataga acacggtgaa agctcagacg acctaataaa
     4261 tagaattaaa catgatatta aaaatgataa aactgactat tggatctata agctaaaaga
     4321 gtttggcata accttataat tttattctgc atatgattga gacagaaaat tatgagcgtt
     4381 agcattaaaa atcaaagaaa tgcttaaata ttcgaaacaa cagaagcact tttaccttac
     4441 ttacagagct taggcttcaa accttgagtg tattgaagaa tacacatatt gtattcttca
     4501 atacgtttta gaatacacag caatacacta tgaatacagc tacaaaatac agtgttagtg
     4561 acgctgccac actgtataaa aaatctcgag ctacactcta taaagatata aaaaacgggg
     4621 tattaagtcg tgatcatgat gggtttatag acttttcaga attacttagg gtttatgggg
     4681 agccatttgg atcaaaacaa gtaaaacgta tcgatacgtc ttcaatacaa aatcaaaata
     4741 cacccgaata catagataaa acagttgtag aactcaagaa tcagatcgat ttcttgaaga
     4801 aacaattaga gaaagccgaa gatcgggaag ccaaagcgaa tgctcgtatc gatacgcttc
     4861 ttacactcat tgaaatgaag tccccacaag ccaatatgac tacgtttacc aatcaagaac
     4921 aacctcaacc gaatgcagct caagtacaac cagaacctag taccgatgat acgaaacatg
     4981 acggattgac tactgtcgag cctaaacgga tttcgatccc tgaacatgtg gaatctggac
     5041 cgaagaaaag agggttatta ggacgtttaa cgaaggcctt ttttgaggac tgattattca
     5101 cctacgctaa agaaaaccgt ggaacattgt tcagttattg atcgtggaac atgagcaatc
     5161 ctaaaaaaag cccaccttgg ggaagatggg ctatcaacta gaaactacag cgttgaatta
     5221 taagggtaat tataacgcat ttcgcataac gtgtattatg ttaattttag gaaatattaa
     5281 aactaatttt cagacttatc ccaccaactt tgagctaaat cagtattttt aggaatacct
     5341 ataccatcac gatacatagc ggaaagactt cgcattgctt tcttattacc taactttgca
     5401 gccttttcac gaagttcaaa agctttatta atattaactt ctattccgct atctgcacga
     5461 gtatatagat cagctaactc aaccattgat tcagaatggc cattttctga ggctttctga
     5521 aaccacttaa aagctttttg gaaatgttct aaagaatcgt ttttgtcatc atcttccatt
     5581 aatctagcat aggtaacata tccatcagct aaccaaaaca ttgctgctac atttccattt
     5641 tctgctgatt taagagccca aacttcagct tgattttcat ctgaatcaac accatgacca
     5701 tatagataaa tctcggctaa ttctaactga gcagaagtat ctccattttc tgctttcgct
     5761 aataaatcat tagaaaaaac aggttctgca aatgtttcca ttgaaaggca aactcctaaa
     5821 gccataaaaa tatttgttaa tttattcaat ttttaacctt taagctaaac tgttaggctt
     5881 gtatattttt atatcatacc taaaagaaaa ctctaaaatt agagtatgtc ttatctctat
     5941 ttaacataat ggctgttata cgaatttcct cccaaaaaat tataaattaa taaatattaa
     6001 atacttataa agagatggat ccggaaattt gaacaactcc tataagtgat actctgctcc
     6061 tcaaatgatg ttataaacat caatatatgg agtattttat ggcacgtaga ccaagaagaa
     6121 atcattcaaa cgactttaag gctaaggtag cacttgctgc gatcaaagca gaaaaaacac
     6181 ttgctgaatt gagttctgag tttgatgttc atcaaaacca aattattgac tggaaaaatc
     6241 aattgatctc agcttcctca caagctttcg atcaatcaaa agctccatca gaacctccca
     6301 ttgatcttaa aaagttacat gcaaaaatcg gtgagcaggc attagaaatt gattttttag
     6361 aaggtgtgtt gaagaaactg ggccgcttca accacaaaag ttaatcgatc actcacttca
     6421 gatttcagta tctaaacaag ctaagttact gaaagtctct cgtggttgtt attattatcg
     6481 cccaaaacct gttagctcat cagatctgaa gctgatgcga tgtattgatg aattacatat
     6541 gcaatatcct tttgcaggca gtcgtatgat gcgtgatttg ttgaatcgtc aagggcatca
     6601 tataggacga cgtcatacac gtactttaat gaagaaaatg ggtattcagg cgttatattg
     6661 caaaccaaat ttaagccagg ctaatcaagc tcaccgtaaa tatccatatc tgctcaaagg
     6721 attggctatt cagcgcagta atcaagtgtg gtctacggat ataacgtata tccctatggc
     6781 aaaaggcttt gtttatttat gtgctgtgat tgattggcat agccgcaagg tacttgcgca
     6841 tagggtatcg attagtatgg aggtggattt ttgtatttcg gctttaaatg aagcgattga
     6901 aaaatatgga tcacctgaaa tattgaatac agaccaaggc agtcagttca ccagtgatgc
     6961 atttattgat gtattgaaat caaatggcat tcaaatcagt atggatggta aaggtcgatg
     7021 ggtagataat gtgatggttg aacgattatg gcggagcgtt aaatatgaag aggtgtatct
     7081 caaagcttat agcagtgtca cagatgcgaa aaagcaattg agtgcatatt ttgagtttta
     7141 taatctgaaa cgacctcatt cgagtctaga caaaatgaca ccaaatgagt tttactatga
     7201 tcagctaccc caacaaaaca aggtggctta actagagcgg aatatcactt ataaatacgc
     7261 ttttagttgt tcaaacaagt ggaaccacct cttaaaacct gttaatattg taaataattc
     7321 ttattcactt taataaaatg aaagaaaaac tccgatggat cattcttgtt gtaagtttag
     7381 cttttttata tggggttact tactttatat ttatcaatac ttctactgga aagcccattt
     7441 ttggggggat acccgaaatt ttggcatata tagttcctgc atactttatt acaagcattc
     7501 tcatcgaagt tctacttaat gatataccta ataaaaatgt aaaattaatg cttggtaaag
     7561 cacttatact tttgctttct tttttagcct ttacagtaat ttattggcta ttttacaaaa
     7621 acatcattat tgctaatgag ttagaaaaca cgtttattgg gaaaaactca cacttcattt
     7681 ttatttggct gatgattttc ttcggtatca aatttgccaa caaattgtac tttaaatgaa
     7741 taacatggcg ctactatgaa tcacttatca ataaatactc agaataaccg gttaattaaa
     7801 tcttggtttg gatttttttt agggttaatt actgttggac ttagtgtgaa tgggctatct
     7861 gaatctttta cattatctag aattctaagc actttaggat ttttatgttt ttggtatccg
     7921 tggactcaac ttacttggaa tcaatcaatg aaagagctta ttcaaattaa tagccaatct
     7981 atgggcaaat catgtattta cttaggattc attgccttga tcttgttctt aagttctgca
     8041 cttgtacgtt tactatgaag acatctaatc tccatttaac ataaaggctg ttatacgcaa
     8101 taaaaaaacc tgggcaccaa cccaggccta aaatttaaaa aacagataaa tatcagtgca
     8161 ataaactcat ttttctaatt tcgcataatg tatattatgt aaattttagg aaaacttaaa
     8221 ttttaatcac ttagtgattt tgatattatt gataataatt ctcaattgaa gaatttaaat
     8281 gaagcaatca aaagataaaa gtttagctgt agcatatttt ttacttgtta catgtggatt
     8341 cttaggtatc catagatttt acctaggaaa aaacattagc ggtattctat ttattattct
     8401 ttcttattca tatttacctt ttacaccagc aatcatattc gttggtattt ggctaataat
     8461 agatattttt ttattaccta cattagttaa gtcaacaaac tctttaaaac atgccacatg
     8521 tgatcctatt taacataatg gtggttatac gcaatacact tgtatattaa tataaaaatc
     8581 aatcgcttaa aaacttcacc ttggtcgcaa aaacagtaaa aagccgactt tgaaacaagt
     8641 cggctttttt atgagcagtt ttgatatttt ttgccaacat ttttgctcaa aaaacactca
     8701 aaacagaaaa tcacctacaa tctataaggc tttcagcctg aaaaaaatcc acaatttctg
     8761 cggataaatc tggggctatt tttaagccat ttttgtacgc tttgacgtac agtaatttaa
     8821 ggaatctgct tctagctgat caaagcgtta tttcttatga tgaagtcata gagagcagga
     8881 agctccaaac aataagaata agaaagatca gcacaaggat ggttaggtac gacaggagtt
     8941 ataccttgca accaaacacg aaaaaccccg acaggatgtc ggggtttttt attgctcaga
     9001 tatttataaa tcaggtttat aaaaaatgat tgaatgatga attttatagc atttaaaccc
     9061 taataaaact gctatcaaca ataagcatcc ctttaagttt atacttgcga tacttttgtt
     9121 tcactaggtt agctacgaaa tatgaatctt atgactgttg agcacataag acagactacg
     9181 aatgctaagt taaatcccaa gaaaaagtct gaacttgggc agttcatgac gcctagtgtt
     9241 attgctgaat atatggcaag ccttttcgat gactataaaa aagaagtaaa acttctagac
     9301 tgtggcgcag gtataggttc attaactatt tctgctgcaa aaaagcttaa aaatatcaag
     9361 ctagcagatc tttgggaaat tgatcccatc atgcgagaac aattagaaat taatatgcat
     9421 gctataaagc ttaatttctt catctatgct caagacttca ttttaggagc tgttgagaac
     9481 atctcttcag ataaaggaga gcgatacact catgcaatta ttaaccctcc ttataaaaaa
     9541 attaatagta attcagaaca tcggagagaa ttaagaaagg ttggaatcga aactgttaat
     9601 ctctattcag catttgttgc actaacaatt caacttatgg aaaaagatgg acaaattgta
     9661 gcgattattc caagatcatt ttgcaatggt ccttattaca agccattcag agaattaatt
     9721 ctgaaggagt gctctataga gcacattcat atctttgaaa gtagagacat ggcttttaaa
     9781 gatgatgatg tcttacaaga aaacatcatc attaaactga ctaaaggtaa acagcagtca
     9841 gatgttcaaa tttctcaatc taatgatcag accttccgtg actaccaaag caaaaccgtc
     9901 ccttttgttg aagtcattaa acctaatgat ccagaactat ttatccgcat tgctacgaac
     9961 gagcagcctc aagaagaaaa taatacgttt tttgccgttt cattaaatga actgggaata
    10021 tctgtaagca caggaccaat tgtgtctcac agaatgaaag agttcttgga gcaatcgcct
    10081 caagatggag ctgttccatt gttttaccct catcatttcg tagacaagca acttcaatat
    10141 ccaaaagagc ataagaagcc aaatgctatt agggttacac cagattcaca gaaatggctg
    10201 ctaccgaata atggctttta cgtgattgtc agacgattca gttctaaaga agaaaaacga
    10261 cgaattgttg ctaatgtcat agatcccaat atgattgata caaattggat cgggtttgat
    10321 aactgttgga atgtatttca tataaaaaaa caaggctttg actatgaaac agcaatggga
    10381 ttggcttgtt ttctgaatag tagcttatta gacaactatt tccgtatttt ctcaggtcat
    10441 acccaagtaa atgcaacaga tctcagaaat atgaaatatc catcacttca gaatttgcag
    10501 cttttaggca aaaaatacaa cattaaaatg gatcaaaaac agattgataa ccttctcggg
    10561 gagattacgt gaataatcaa gatacaaagc tgcagcaagc atacgatatc cttgtccaat
    10621 taggcatgcc gcgccaacaa cttaatgaac gtactgctct ctgtctactt tgtttacttg
    10681 atatgacacc tgaaaaatca tggaatcagg caagtaaccc cttagtaggt attacaccta
    10741 tcatgaactg ggcacgtgac cattatcaaa gagaatacgc tccaaataca cgagagacat
    10801 tccgtcgaca atcgatgcac caatttattg aagcaggagt ttgtctttat aacccagata
    10861 agcctgatag acccgttaat agcccaaaag ctgtatacca aattgaattc aatttgcttc
    10921 aagttcttca atcttatgga acagagcaat atgaaaccat gctacagaat tacctgcagc
    10981 aaagacaaac tcttgctcaa atgtatgcac gagagcgtga aatgaatatg atcccattac
    11041 aactaacaga tggtcagact attctacttt cagcaggtga ccattctcag ctcatcaaag
    11101 atattgtcat cgattttggt gctcgatatg ttcctggggg agtattagtt tatgcaggtg
    11161 atacaggtaa taaacatggg ttctttgatg ttgagctact agcatctttg ggtgtgcaat
    11221 tagacaacca cgggaagcta ccagatgttg ttatctacaa tcctgagaaa aattggttat
    11281 tcttaatcga agctgttacc agtcatgggc ctgtagacca taaaagatat ggggaattaa
    11341 atacactatt ccaaaactgt accgcaggtt tagtttttgt ctctgcattt cctgatacaa
    11401 agacctatgt aaagtatagt ggtgttattg catgggaaac cgaagtatgg atggctgaca
    11461 atccttccca tatgattcat ttcaatggtt ctagatttat gggaccatac taaaaattaa
    11521 atatatttta ggatgattaa atacattgtc tttttctaat atcgatccaa aaagtgatta
    11581 tgagttttca aaaattttaa acaaatttgt aactccttct gaaccaataa caactccaga
    11641 aaggttattt ggaagagaag atgagttaaa gagagcatca agagctctat tagatccggg
    11701 taaacaaata tttattattg gagagagggg agttggtaaa acatctttag ctaatattgt
    11761 agcaagtttt catcaaagtt cagatgctga acccctaaaa actagttgtg ctgaagatac
    11821 tacttttaga actttaattt ctgatatcgt aataaaagca ttaaatagat caaaatttaa
    11881 agagatatct actaaacaaa caaaaggtat ttcatttcct ctattatcga agatatccgc
    11941 taatcttggt caggaagtaa gtgtaaaaga gaaagttatt ttagatcaaa tagatactgt
    12001 atcagatgct ttaacaattc ttcaagaaat atcagaactt cactcagaga atccagttat
    12061 tgttgttgat gaattagatc gtatgaataa agataatata cgaccattat cagacataat
    12121 aaaaggaatt agtgatcaaa gaattaatat taagtttata tttacagcag tagctcagac
    12181 ttatgaacta atatttggat cacatgagtc gactagaaga caattagaaa ttatagaatt
    12241 accagtttta agctgggatt ctcgaatgga gcttattgaa aaaaccttaa ataattttga
    12301 tattagtatt gatagaacca ccagtataag gatatctgct ataagtgatg gatttcctca
    12361 ttatattcat tttatattaa agcaattatt atggaatatc tttgaaacta aagaggtagt
    12421 gactgaaata gatttatctt catataacat tgctttaaat gatgttgtaa aacaattaag
    12481 tcctgaatac tccttaaaat atcatagagc aattaataca agacacatag attgtgaact
    12541 agttctttgg tcaacctcta ttggtgagtg gattattaac tcattagatg aaatgtttga
    12601 agcatattta gaaatatttt atttattacg tgatgaacaa gatatccctc tagaaaaaac
    12661 aaaatttaat agcatggtta gagacctaac aaaagctcaa cgaggaaaga tcttagtttc
    12721 atctcactca ggcaaaagag gtgaatatga gttttcggaa aagatattta agggctatat
    12781 aagaatacaa gcagaactac attctattaa tataaaaggt atagatgaga agggccttat
    12841 aactccaatt ctaacacaat ggtcaaatat ccctttagca agaacaggat atcatcagtc
    12901 aaaaccgcct caaggctatc caagaaaaag tagtgttttt ggaaattata tgtaatttaa
    12961 tataagagaa gaaaaagtta attatttaac ataaaatctc ttatgcgaaa ttgaattgaa
    13021 ttgaagtgtg agaactcatt tctagctatc ttataggcct aatgcagtag tttccgctgc
    13081 catatcttcg cttaagtacc atatgtatat acccagttgt agaaggtttt agaggatcca
    13141 ataacctgat tttagaaggc cccggattgt aacgagccac tgctttcgga tcttcaactt
    13201 ttaaacgctc tacttcttca ttgataacat cacgggccac gtactttaaa acactataag
    13261 cctctctttt attaagaaat ttgaaagagg aacttccatt ttcctttact cctaaacgga
    13321 agtgcttatc taaaaattta gctaaatcat cagcagaacc taaaaaaggt ttcggcataa
    13381 tattctcctt aaactaattc ttatattatt cagtattaga actgcaaact tttttaagta
    13441 tcaatcaatt aacaacttta agttaaataa gatattgatt aatcaattga ttataatttt
    13501 cttcatacac attttcctta ccaaactctc gtcctgtttc ttcaaaaaga cgtcttaaaa
    13561 tcacgaattg catgaagtct
//
