LOCUS       NZ_CP045021             5251 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain BK13048 plasmid
            pBK13048-7, complete sequence.
ACCESSION   NZ_CP045021
VERSION     NZ_CP045021.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12984737
            Assembly: GCF_009025895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Chen,L.
  TITLE     Genomic sequencing of K. pneumoniae strain BK13048
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2019) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAY-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:43:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,393
            Genes (coding)                    :: 5,265
            CDSs (with protein)               :: 5,265
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 60 of 128
            Pseudo Genes (incomplete)         :: 63 of 128
            Pseudo Genes (internal stop)      :: 16 of 128
            Pseudo Genes (multiple problems)  :: 11 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045021.1.
            Bacteria and source DNA available from HMH-CDI.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BK13048"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pBK13048-7"
                     /collection_date="2003"
                     /collected_by="CDI"
     gene            complement(join(4884..5251,1..118))
                     /locus_tag="F9865_RS27510"
                     /old_locus_tag="F9865_27525"
     CDS             complement(join(4884..5251,1..118))
                     /locus_tag="F9865_RS27510"
                     /old_locus_tag="F9865_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<57..833)
                     /locus_tag="F9865_RS27515"
                     /old_locus_tag="F9865_27530"
                     /pseudo=""
     CDS             complement(<57..833)
                     /locus_tag="F9865_RS27515"
                     /old_locus_tag="F9865_27530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(796..1116)
                     /locus_tag="F9865_RS27520"
                     /old_locus_tag="F9865_27535"
     CDS             complement(796..1116)
                     /locus_tag="F9865_RS27520"
                     /old_locus_tag="F9865_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1753..1914
                     /locus_tag="F9865_RS27530"
                     /old_locus_tag="F9865_27545"
     CDS             1753..1914
                     /locus_tag="F9865_RS27530"
                     /old_locus_tag="F9865_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(2067..2687)
                     /locus_tag="F9865_RS27535"
                     /old_locus_tag="F9865_27550"
     CDS             complement(2067..2687)
                     /locus_tag="F9865_RS27535"
                     /old_locus_tag="F9865_27550"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(2680..4035)
                     /locus_tag="F9865_RS27540"
                     /old_locus_tag="F9865_27555"
     CDS             complement(2680..4035)
                     /locus_tag="F9865_RS27540"
                     /old_locus_tag="F9865_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            complement(4668..4880)
                     /locus_tag="F9865_RS27545"
                     /old_locus_tag="F9865_27560"
     CDS             complement(4668..4880)
                     /locus_tag="F9865_RS27545"
                     /old_locus_tag="F9865_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1231..1403)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          1350..1863
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..806)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.045"
                     /coverage="99.0"
                     /evalue="1.6399999999999998e-152"
     Typing          complement(4553..5250)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.02e-142"
ORIGIN
        1 ccgcttttac agactgctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct
       61 gctgctgcgc tttcgattgc tgctccaagt ccttcgccag cgctaaaaga ctgctcataa
      121 atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttt
      181 tttgtggtgc ggaccacctc aaaacctgcc gctgtgagcg cccctgtgac gtcctcgcgc
      241 gttttgagct ccccggatga ggctaaggcc agcaacccac gtgtaatcgc ctgagcggct
      301 tccagcttcg tttccggcag tgcggacggc gtgaccagta accgtcggtt ctccggggcg
      361 tttggatcat gcagccccag cctgccgttc accacggtct gccaggcatc gatgcgcggc
      421 cggtcggcgc gatcgtagta cggctgcagg cgtttcccgg tcagcagttc agtgttgggg
      481 atcaggaaat tcagctccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac
      541 tggtctttat cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc
      601 tgcccgggcg gcagttcagc ctccgcgaac gacagcacgc cggaggtgta ctttttcgca
      661 tacggcgagg catcgataag ctcccggact tcctccggtt ttccctgcag cacggtcgcc
      721 ccctcgcgct gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgc
      781 ccccgggggt gaaatttaac gatcatcatc cccccctttt tccagcaccg catgccgcag
      841 ccgctccagt cccgcgtcga ttgccatcag tgcggccaca atctgcaccc ggtcatgacc
      901 ggcaccaccg gcattcactt tccgggcgat ctggttcagg ttgttcccca taccggcaag
      961 ctggcgcagc agcgccggcg atatcgacgg cagtctgccg gcacgcgccg gcttctcatc
     1021 gaggcaggtc tgacgcatcc acgccgccag ctgcttgccg tcgcaccgct cgagcagccg
     1081 ccggtgttcg tcttccgtca cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt
     1141 agcctcctga taacaccggg acgggcggga aatgtttccc ggtcggcacg gtgttggaca
     1201 agccgcaggc gcgtcagtcg cttttagcgg gtttcggggc gcagccctga accagtcacg
     1261 tagcgctagc ggagtgtata ctggcttatt atgttggcat gagtgcggta atcaggaaag
     1321 tgcttcatgt gacagaggaa aatgcagctg cgggcgtaag gcagaaaatg gaagttcaga
     1381 ggatatgacg cctcctcgct cactcagtcg ctgccgctcc tgccgttcgg ctgcggcggg
     1441 cgttacctgc acacggagaa gagcgaaggc atatggcatg acaaacgggg cagaaaaagg
     1501 atgcgccgct gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1561 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1621 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1681 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1741 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt ctccggtaac
     1801 tatcatcttg agtccaaccc ggaaagacac ggttcatcgc cactggcagc agccattggt
     1861 aacggattag aggaagtgag tcttgaagtt ctgcgccagt taaggctaaa ctgaaaggac
     1921 aatttttggt gactgcgctc cctctaagcc agttacccgg tacataagtt ggtagctcat
     1981 gtaccttcca aaaaccaccg ttggtagcgg tggtttttat ttttcataga ttacagcctg
     2041 taataagcca tggtcaatgg attacttcaa aagtcgtcaa tcgccttaag tagctcatgg
     2101 aatgatgtat aacatggaaa tagataaggt tcgtgcgtgt ttttataatt agttttagcg
     2161 taaatatcta gctttcttgc gttatctttc gcagtaccaa tcctctctct tatagcaagg
     2221 taagttagtt tactaccttt atcatagtta gttattaatt tttttagcgg ggagctagac
     2281 atcaaattat catacgacga aaaatctttg gttgtgaagc cgaaatgcaa caataaccat
     2341 aattcaaaac aaacacagga tattgctata tttatcccat gcctattagc tttattccat
     2401 gccttttgat gacatgaaat agacgaaggc gtcagatgct cttgatcata aacaacccaa
     2461 aagacgtcac cttctgcgga agatgatgaa ttctttttct ttatcgcctc atccaccaac
     2521 tgctcaggtg tatttttcct tgttttaggt attttaaata cacttaaagt tcttttagat
     2581 ctgtctgaaa tataagcctc aaggtataat ggttcggttt tctcaccctc acagtatata
     2641 tacattactt gtgggacttt cttattattt ccatgttttt tactcatcag aaagatcccc
     2701 gtcaaactct tcaaaaaaac catcagacaa ttctttgacg tcaatatccg gcattaccaa
     2761 ggatatatca ccagatataa atgaggctac ctttaagtag tttatgtcgg gaaccccacc
     2821 aaacctaccc tcatcatacc aagctgcgta aggtgttgtt gtttttatct ttttcttatc
     2881 aaaatcatct aagctataaa gagacgacgc acctttattt ttttcacaaa accatatctg
     2941 atctcttctc attttctctg gagccattaa ctgcatatta tgggttgaga atacaagttg
     3001 tgagcctttt ttattgacat caggatcatt aaataactta acaatcaagt cagcaatatg
     3061 cgggtgcaag ctattatcta actcatcaac aattacaacc atactatcat taaacgcaga
     3121 tctaattaga ggtatgattt caaataattt ttgggtgcct tcagactctc tccccatagg
     3181 gaaagaaaca ctcccccctt catcagtagg atgagagaac atataacgat atttataatc
     3241 ttcttcaatt atctccttta tttcttgagg gatttcctca tccattttta ttggtatttc
     3301 cgcctcaacc tcccgtacag atataccgga aacaccagtg tcaaccatgc ataaaacctt
     3361 ggccgtttca tttacgacgt cctccctctc cccaaaagaa gatattctta tcttttcatt
     3421 taaacctata tgtctaattc cctttctaaa aaagttataa gcttctttaa ttatgtctgg
     3481 tgatgcggca ttattacctg ctttcgctaa gtatgaatta ttagggaaga atggtatttt
     3541 tttaatacct cctttataat gcccaccaaa cttgattgtt tcccaggtgt cgctctcatc
     3601 tctagtaaat aaatttgctt taaccctaga tggataatag tctaatgatt caaataaaat
     3661 ccgattttta ataaaagcaa cttcataact gaaccgattc ccctcaacag aataaaattc
     3721 agcctcaaac tttacaggag cctcattcgt agccgaagaa agagcatatg gctcatagca
     3781 gggaatccgt ttaccttctt tcaaatcacc gctttctgtt gcgataaatt ttatagcttc
     3841 aaaagccatg agaacgttag atttaccaga cgcattagca ccataaatcc cagcactcat
     3901 gagcgttcca acattcattc catcattttt atagatatga gttgttaaat ggttactaga
     3961 accggatgcg ctcaagttta atgtcgattt ttccttaacc gacctaaaat tttcaatagt
     4021 caaataaaca aacatgcata cctcctaacg gggatttttc gccaaaaaag cgaaaaatca
     4081 atcataggaa agatatccta tatgttttaa ttttggaaca acatcttcct taaaaaacgt
     4141 tgtgctattt ataattacac gctatttacc ataacatctg ttgcccgcac cgctccggac
     4201 aggttcgcca caaacggggc ggcagtcagg taaaaccggc aacaaaccac ccgatacacc
     4261 gccccgttcg tgacagataa attttacccg gctgcatggt catgcagcca gatgcatacg
     4321 gtctgatttc aggccagaat ccggatgaac gccttaaaaa caggaaaatg cttctccagc
     4381 cagtcccaga tctccagcgc catgtacagg caattcagca ccgtctcaat cacgttaaac
     4441 agtctcataa tccctccgtt gtatacgctg tgatggaaac atcacgaccg tatttagcgg
     4501 gaaatttatg agcccttctt cttggggttt gtcactggat gcgcgcagtc agcccagcgt
     4561 cggaccgtca tagctccgct gccgggaacg ggtttccgcc agctcttttt catgctgttt
     4621 ctgctcgtat gcccgttcgg cggccaccgc catttcatgc cgctgcacta ctggctcaaa
     4681 cgctgtaact gcctgctcaa gttcgccacc tgctgactca agcgcgtgac gcgctcgttc
     4741 agcgccgcgt tctcccgttg cataagacca gacatcttct gccattccgc gaaggcgctc
     4801 tcccactcgt ccagcctttg cgagtagtcc tgatgtagct gctctaatgc ggtcagcaac
     4861 tgtttttcca gttccgtcat gggctatttc ctctccagta ccatccagtt cccttcctcc
     4921 ccgtaagctc ccgctttcgg gttcactttg acgcaggaca gtttcttctc cccgcagagc
     4981 gacagctgca cgccgtggtt cttctccgac agcatggcct gcgtccgctc ctgcgcccgg
     5041 atagtctggt aattcccggt gatctgctgc ccctgccacc acagaatgct cccgctcgtg
     5101 gcgatcagca gtaccgagac cagggtgatg gtcagccacg tccggccgac catacgcagc
     5161 acgctcagcc ggttcgactg catcgccgcg gtcataccct gctcgtgggc gctgatggcg
     5221 tcgctgattc tcttcgcgct tgcgctcagt t
//
