LOCUS       NZ_CP044531             1180 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella grimontii strain SS141 plasmid plamid_3.2.
ACCESSION   NZ_CP044531
VERSION     NZ_CP044531.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10462700
            Assembly: GCF_004104525.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Hubbard,A.T.M., Reine,J., Newire,E., Botelho,J., Wright,E.,
            Murphy,E.A., Hutton,W. and Roberts,A.P.
  TITLE     Isolation of an antimicrobial resistant, biofilm forming, Klebsiella
            grimontii isolate from a re-usable water bottle
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Hubbard,A.T.M., Reine,J., Newire,E., Botelho,J., Wright,E.,
            Murphy,E.A., Hutton,W. and Roberts,A.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2019) Department of Tropical Disease Biology,
            LSTM, Liverpool School of Tropical Medicine, Pembroke Place,
            Liverpool, England L3 5QA, United Kingdom
REFERENCE   3  (bases 1 to 1180)
  AUTHORS   Hubbard,A.T.M., Newire,E., Murphy,E.A., Hutton,W. and Roberts,A.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2018) Parasitology, Liverpool School of Tropical
            Medicine, Pembroke Place, Liverpool, England L3 5QA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-SEP-2019
            Assembly Method                   :: Unicycler v. 0.4.6.0
            Assembly Name                     :: K_grimontii
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,980
            CDSs (total)                      :: 5,860
            Genes (coding)                    :: 5,725
            CDSs (with protein)               :: 5,725
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 54 of 135
            Pseudo Genes (incomplete)         :: 88 of 135
            Pseudo Genes (internal stop)      :: 21 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044531.1.
            Bacteria and source DNA available from Dr Adam Robets.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="SS141"
                     /isolation_source="Coffee Cup"
                     /db_xref="taxon:2058152"
                     /plasmid="plamid_3.2"
                     /geo_loc_name="United Kingdom"
                     /lat_lon="51.5074 N 0.1278 W"
                     /collection_date="2018-03-01"
                     /collected_by="Dr Adam Roberts"
     gene            503..694
                     /locus_tag="EHW69_RS30295"
     CDS             503..694
                     /locus_tag="EHW69_RS30295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045358396.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            694..1095
                     /locus_tag="EHW69_RS30300"
     CDS             694..1095
                     /locus_tag="EHW69_RS30300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(5..455)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.931"
                     /coverage="88.0"
                     /evalue="4.71e-148"
ORIGIN
        1 accgccaatg gcgatttgtc gtgccttttc gggttggact caagacgata gttaccgaaa
       61 agagcgtggt agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
      121 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat
      181 gacaccggta aaccgaatgg caggaacagg agagcgcacg agggagccat cagggggaaa
      241 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
      301 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc cgctgtgctg ccttctccct
      361 ctccgagctg tctgctctat gtcgttatgt gcgcgttgcg accgctcgcc gcagccgaac
      421 ggcaggagcg tagcgactga gtgagcgagg aagcggaaaa gagatctgtc tgacattgag
      481 cactgacgct ctgaggccct ttatgacgaa acaacagaaa acagcgctga acatggctaa
      541 attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg atctcgatgt
      601 cgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttct gtacactgac
      661 cgcgagactg gatgcattac aggatggtaa ctgatggcca gagtgtctat tagtgaggct
      721 gcccgactga cgggtaagag tagaacaaca ctgcacaggt taattaaagc aggtgacctg
      781 tccacctgta gcggtgaacg taatgcaaaa atggtagata cttcagagct tttacgtgtc
      841 tttggcccct ttgaacaact gaagggtgaa caccattctg gacaggtctc tgaacagcgt
      901 gatacagcct ctgctgacca gagtgaacag gtgattcgtc atctgaaaca ggagattgaa
      961 cacctgaaca ccttactttt gtccaaggat tcgcacattg acagcctgaa acaggccatg
     1021 ctccttcttg aaaatcacca ggcaaaatta actgaatccc cggcgccatg gtggaaattc
     1081 tggaaaaaat cctgaccccg tcatgcttca gttttcctct ccggccatcc cagggagttc
     1141 ctgaaccttc ccaaaaatcg tcagatctct gaacgctcct
//
