LOCUS       NZ_CP044468            11821 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter schindleri strain HZE23-1 plasmid pHZE23-1-5, complete
            sequence.
ACCESSION   NZ_CP044468
VERSION     NZ_CP044468.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12478518
            Assembly: GCF_010918635.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter schindleri
  ORGANISM  Acinetobacter schindleri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Non-baumannii Acinetobacter spp. carrying blaNDM-1 isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) College of Veterinary Medicine, South China
            Agricultural University, 483 Wushan Street, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:25:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 28 of 96
            Pseudo Genes (incomplete)         :: 72 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 23 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044468.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Acinetobacter schindleri"
                     /mol_type="genomic DNA"
                     /strain="HZE23-1"
                     /isolation_source="stool"
                     /host="goose"
                     /db_xref="taxon:108981"
                     /plasmid="pHZE23-1-5"
                     /geo_loc_name="China:Huizhou City, Guangdong Province"
                     /lat_lon="23.12 N 114.42 E"
                     /collection_date="2018-01-20"
                     /collected_by="chaoyuecui"
     gene            join(11319..11821,1..1429)
                     /locus_tag="FSC10_RS16100"
                     /old_locus_tag="FSC10_16225"
     CDS             join(11319..11821,1..1429)
                     /locus_tag="FSC10_RS16100"
                     /old_locus_tag="FSC10_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728972.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_154320142.1"
                     /translation="MIQLDQLSIRRGGRVLFQKASMQLHPGWKIGLTGVNGAGKSTLFS
                     ALLGGMESDTGSLTRPAVWTVAHMAQEIKALNMKAIDFVLSGDEEYWDIQQKLEQPDQL
                     DDAELAHLYGRFDEISGYSAPAKASQLMAGLGFFEHQSQLDVSSFSGGWRMRLNLARTL
                     MSRSDLLLLDEPTNHLDLDAILWLEDWLKAYEGTLVLISHDRDFLDAITDHILHIENQE
                     LILYTGNYSTFERTRSERLAQQQQAYEKQLETRAHLQKYIDRFKAKATKAKQAQSRIKQ
                     LERMQELSAAHVDTPFTFSFREPTKMSSPLLQLEHADIGYGDKLIVTNVNLQITPSSRI
                     GLLGMNGAGKSTLIKSLVGDLKLLQGLRKDSELLNIGYFAQHQMDALDGNASPMLQLAR
                     IADKKISEASLRSFLGSFGFSGERMDTPSESFSGGERARLALALIVWQRPNVLILDEPT
                     NHLDLDMRHALTMALQDFEGAVVLVSHERQLIASVCDELLLVHNGQCREFDGDLTAYAD
                     WLRQARIEMIKNGQKPAEPVKSQVEVKATISRLDKEAQRKEAARQRELSRPIRKNIEKC
                     ETQISKVTPRLAEIQSLLGDSALYDASRKNDLLKLMNEQTELKAKLETAEEQMLELMME
                     LESLEQSF"
                     /name="ATP-binding cassette domain-containing protein"
     gene            1694..2395
                     /locus_tag="FSC10_RS16105"
                     /old_locus_tag="FSC10_16230"
     CDS             1694..2395
                     /locus_tag="FSC10_RS16105"
                     /old_locus_tag="FSC10_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586042.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_154320143.1"
                     /translation="MRITLEIKCPACLSDSIKKNGTKVDGKQNYQCKICRRQFIGDHAL
                     SYQGCHSGIKTKILHLMVRGSGVRDIAEIERVSIGKVLRTLSQSKYQLRAQQSHYETLE
                     VDEFWTFVGHKQNKQWLIYAYHRETGEIVAYVWGNRDLATAKRLKLKLKQLGVSYTCIS
                     SDHWDSFVTTFKECKQLIGKFFTVGIEGNNCRLRHRIRRGFRRSCNFSKKLENHYKAFD
                     LAFFYINNGFV"
                     /name="IS1 family transposase"
     gene            complement(2628..3512)
                     /locus_tag="FSC10_RS16110"
                     /old_locus_tag="FSC10_16235"
     CDS             complement(2628..3512)
                     /locus_tag="FSC10_RS16110"
                     /old_locus_tag="FSC10_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(3568..5043)
                     /gene="msr(E)"
                     /locus_tag="FSC10_RS16115"
                     /old_locus_tag="FSC10_16240"
     ARG             complement(3572..5043)
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5714..6256)
                     /locus_tag="FSC10_RS16120"
                     /old_locus_tag="FSC10_16245"
     CDS             complement(5714..6256)
                     /locus_tag="FSC10_RS16120"
                     /old_locus_tag="FSC10_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_004896925.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFSEALRV
                     FGEPQKKEDTVNQSQSINSQNLTEVDLLKRQVDILEKQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLEAPKANMTTFTDQSFKQDIATDPRSELATNYDELTTPQQDNKRIPIPEHVE
                     PEQKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6323..7252)
                     /gene="repM"
                     /locus_tag="FSC10_RS16125"
                     /old_locus_tag="FSC10_16250"
     CDS             complement(6323..7252)
                     /gene="repM"
                     /locus_tag="FSC10_RS16125"
                     /old_locus_tag="FSC10_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068553070.1"
                     /translation="MKTELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNTINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITMYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDIIVKVEQHKTGRSITGF
                     SFSFKQKKSATHSVESKRDPNTLDLFSKITDKQRHLFANKLSELPEMSKYSQGTESYQQ
                     FAVRIAAMLQDAEKFKELLPLLRKLGFQ"
                     /name="repM"
     gene            complement(7455..7688)
                     /locus_tag="FSC10_RS16130"
                     /old_locus_tag="FSC10_16255"
     CDS             complement(7455..7688)
                     /locus_tag="FSC10_RS16130"
                     /old_locus_tag="FSC10_16255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068553069.1"
                     /translation="MGANFPNLIPKPSVARKKAHERSEFRAAFDLAFAFSQIHENMNQK
                     LPRRIERKRDSIEFERSENQGQFFTPWAFNYF"
                     /name="hypothetical protein"
     gene            complement(7790..9226)
                     /gene="mobQ"
                     /locus_tag="FSC10_RS16135"
                     /old_locus_tag="FSC10_16260"
     CDS             complement(7790..9226)
                     /gene="mobQ"
                     /locus_tag="FSC10_RS16135"
                     /old_locus_tag="FSC10_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_107111964.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNRERLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTQEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDLAGYTARVDHRSYKDQENGLEATLHEGPKVTELRRKG
                     IETEISRSNDEIKQRNQAQLQYSKNMDLLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLGKVLKREISAKDANLDLDFMQRNLKQAEITLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDAIYQEHKKLKGQHEHQK
                     KHGVKDLLENDKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVDEIKAEQQMKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     gene            9435..9701
                     /locus_tag="FSC10_RS16140"
                     /old_locus_tag="FSC10_16265"
     CDS             9435..9701
                     /locus_tag="FSC10_RS16140"
                     /old_locus_tag="FSC10_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_088749624.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="mobilization protein"
     gene            9719..10150
                     /locus_tag="FSC10_RS16145"
                     /old_locus_tag="FSC10_16270"
     CDS             9719..10150
                     /locus_tag="FSC10_RS16145"
                     /old_locus_tag="FSC10_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039090891.1"
                     /translation="MEDPTLNKLIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFVKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSIITIGFERDTAYLANALEVFLISKIQPVHNVVKVGM"
                     /name="hypothetical protein"
     gene            complement(10389..11090)
                     /locus_tag="FSC10_RS16150"
                     /old_locus_tag="FSC10_16275"
     CDS             complement(10389..11090)
                     /locus_tag="FSC10_RS16150"
                     /old_locus_tag="FSC10_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586042.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_004282341.1"
                     /translation="MRITLEIKCPACLSDSIKKNGTKVDGKQNYQCKICRRQFIGDHAL
                     SYQGCHSGIKTKILHLMVRGSGVRDIAEIERVSIGKVLRTLSQSKYQLRAQQSHYETLE
                     VDEFWTFVGHKQNKQWLIYAYHRETGEIVAYVWGNRDLATAKRLKLKLKQLGVSYTCIS
                     SDHWDSFVTTFKECKQLIGKFFTVGIEGNNCRLRHRIRRGFRRSCNFSKKLENHFKAFD
                     LVFFYINNGFV"
                     /name="IS1 family transposase"
     ARG             complement(2632..3512)
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3569..5043)
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2629..3512)
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6324..7252)
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.108"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7794..9226)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.05"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttactcctt cttgatgaac cgaccaacca tttagacctg gatgccattc tctggctcga
       61 agactggctg aaagcctatg aaggtacgct ggtgctgatt tcgcatgacc gggatttcct
      121 cgatgcgatt accgatcata ttttacatat tgaaaatcag gaactgattt tatataccgg
      181 taactactcg accttcgagc gcacccgcag tgaacgtctg gcgcagcagc aacaggctta
      241 tgaaaagcag ctggaaaccc gcgcacatct gcaaaaatat attgaccgtt ttaaagcgaa
      301 agccaccaaa gccaagcagg cacagagccg gatcaaacag ctggagcgta tgcaggaact
      361 gtctgctgcc catgttgata caccatttac cttcagtttc cgtgaaccga ccaaaatgag
      421 ttcgccttta ttgcaactgg aacatgccga tattggttat ggtgacaagc tgattgtcac
      481 caatgtcaat ttacagatca cgccaagcag ccggattggt ttgctcggga tgaatggtgc
      541 cggtaaatcc accctgatta aatcgctggt cggtgattta aaattgcttc agggtctgcg
      601 taaagattca gaactgctca atattggcta ctttgcccaa caccaaatgg atgcactgga
      661 tggcaatgcc agcccgatgt tacagctggc ccgtattgcc gataaaaaaa tcagtgaagc
      721 cagcctgcgt tctttcctgg gcagttttgg ttttagcggc gaacgcatgg atacgccaag
      781 tgaaagtttc tcaggcggtg aacgtgcccg tctggctctg gcattgattg tctggcagcg
      841 tccaaacgtg ttgattctgg atgagccgac caaccattta gatctggata tgcgtcatgc
      901 attgaccatg gccttgcagg attttgaagg tgctgtggtg ctggtatcgc atgagcgcca
      961 gctgattgcc agtgtctgtg atgaattgct gctggtacat aatggtcaat gccgtgaatt
     1021 tgacggtgat ctgaccgctt atgccgactg gttacgtcag gcccgcattg agatgatcaa
     1081 aaacgggcag aagccagccg agccggtgaa atctcaggtc gaagtcaaag caaccatttc
     1141 cagactggac aaagaagcac agcgtaagga agcggcacgc cagcgggaac tgagccgccc
     1201 gattcgtaaa aatatcgaaa aatgtgaaac acagatttca aaggttacgc cgcgactggc
     1261 tgaaattcaa agcttgctcg gagatagtgc tttatatgat gcctcacgta aaaatgattt
     1321 gctcaaactg atgaatgagc aaactgagct gaaagccaaa ctggaaacag ccgaagaaca
     1381 gatgctggaa ctaatgatgg aactggaaag tctggagcag tccttctaac actatttcaa
     1441 cacccccatt atctgagaga tgagtaacaa gcgttattca tctctttttt ttattaaaac
     1501 cacccatttt taatattttc ctcctgcttt tcatctccgt gtagttcctt gctattcttg
     1561 agtactgcat ctcttcttca aaacttgaca cattttgtcg tgaattacgc aagtgccata
     1621 taagtttatt ttttggtggt gtttcaaaaa gtatgctgaa aaaaagcagg ttggattttg
     1681 ataaaataga gaaatgcgaa taactctaga aatcaagtgt ccagcctgcc taagtgacag
     1741 tataaagaaa aatggcacaa aagtagatgg taagcaaaat taccaatgta aaatatgcag
     1801 acgtcaattt attggtgatc atgctctcag ttatcaaggc tgtcactcag gtatcaaaac
     1861 caaaatatta cacctcatgg tgcgaggcag tggtgtcagg gatatagcag aaattgaaag
     1921 agtaagtatc ggtaaagttc tacggacact tagccaatca aaatatcaac ttcgagcaca
     1981 gcaaagtcat tatgaaaccc ttgaagtaga tgagttttgg acttttgttg gtcataagca
     2041 aaataaacaa tggctaattt atgcctatca tcgagaaaca ggtgaaattg ttgcttatgt
     2101 atggggtaac cgagatttag ccactgcaaa acgacttaaa ttaaaattga agcagttagg
     2161 tgtaagctat acatgtatat caagtgacca ttgggatagc tttgttacga cctttaaaga
     2221 atgcaaacag ttgattggta aatttttcac tgtgggtata gaaggcaata attgtcgatt
     2281 acggcatcgg atcaggcgag gctttaggag aagctgcaac ttctccaaaa agcttgaaaa
     2341 tcattacaaa gcctttgatt tagcgttctt ttatatcaat aatggattcg tttaatgcca
     2401 gcatactttt tgaaacacca ccaaattttt tatatcaaaa tttcaaagtg tactgatgag
     2461 tacactttga atgggttcta atacccgaat ttcgtatatg aaattttatg ttaaatgatt
     2521 gttagaaatg ccccttgatc gaggcatttt ctataaagaa tactaaaaac taattaaagc
     2581 caaccaagtt tcaaaacaag gcagttctca gcaatcatat ttttaaatta tataactccc
     2641 aactgagctt ttgctccaac gataaggctt tcattttgag tttctaaggc aaataaacca
     2701 tacatcaaag gagaggccgc tgctctttct aaagtctgtt catatagttt attccaaact
     2761 ttacccccta gtttttcata ctcgatgatt agagttttga ggctttcttc tccaaacaaa
     2821 gttacatgcc cagcaaaatc aatcgctggg tcatctatat gggctgttga ccaatcaata
     2881 acgcctgaaa cagctccatc ctttgaagct agtacatgcc cagcatataa atcgccatgt
     2941 ataaattggg tgaaatctgc ccatagaaca tcattatcca accattttct gtagcgggtt
     3001 tccaattgct cacttatacc aatttcagat tttactaact gcaaattgtt tgctatttca
     3061 ggtcttaaat ctgaaggttt cataattttc aaatcatttt cccgaacttc tttttcagga
     3121 atactatgga tttcaaataa ggtttttgcc aaagatgtta tgtatttcgg gctatctttg
     3181 tccatattcc aaattatttc ataggtttca gcatccaaat ttaaaacagg attatcttta
     3241 agtatgggat aagccactaa ttctgtagat gaaattctcc aatcaggaac ctctacagaa
     3301 agatgttttt ttaccaattc taaaatgcgt ttttctttct tgatttgttc cctcatgcca
     3361 tcacgacgag gaatacgcag caaccattgt tgcccctttg tatcaagagc aaaaacgacc
     3421 ttaaaatcaa tgcccatttc attgaaattc attttgtccg taagcaacaa gccgtgtgct
     3481 tcagcaagtg attgaatatc ttgaattgtc atttttaatt tcctttaaag agttcaataa
     3541 ttaatgttcg gattagattg gctatcatta acaatctctc tcaaaagtct tgatgatttt
     3601 gtggtctttg atctcgtaga taatgtcagc aatattatcg accaattgct tgtcatgaga
     3661 tacgaagata atagttcctg cataggactt catcattgtt tctaatgcgg caatactttt
     3721 taggtcaaga tagtttcctg gttcatccat aagcaaaata ttatattttc ctaaaagcat
     3781 tttggataaa agcagtttga tgatttcacc tcccgataag tcggataagt ttttttgaat
     3841 atcattcgct ccgatcccca ttgaagccaa tactgcacga atttccgcaa ctgtgtactc
     3901 gcactcttcc tgcataaagg agagcacaga tttatgcgtg ttaaatttat atcctgtttg
     3961 tgtaaagtag ccaatttcag cttttggaga tatggttaat ccatcagcac gttctgatat
     4021 catttttaac aaggacgttt tccctgttcc attcgatcca gttatagcga ctttagcgcc
     4081 aagcggtatt ataaagttag cgtcatcaaa gatagtacgg ctaccaaatt ttaagctcag
     4141 accatctgcc gtaatcggga acttattgtg cagttctagg gctgaacttt gacgaaaacg
     4201 aatagaacgc aaatgctctg gtgcttgaat atcttctaat gcagccaaac gcttttccat
     4261 actcttagct gcctgataca gttttctttg cttggtgcca gtcatttttg catgcccaag
     4321 tcgtccagca ctttcggtag agtttttgga tttttctcct tttttcttat tgtctaatcg
     4381 attagcttgc tggcgttttt cttgcacagc agattctaat cgctcccgtt ccttcatcat
     4441 cagctcatat tctacggctt ggtgttgtcg ctcttcttct ttttgacgca agtaatccga
     4501 gtaaccaccc caatattccg taattttacc gtcttttaac tcccatatct tgtctacaac
     4561 catatcaaga aaatatcggt catgactgat aacaagtaat gctccatcaa atgctttaag
     4621 ttgaccaata agtagatcta ttccattgag atcaaggtgg ctggttggtt catccgctag
     4681 aatgccatgt acttgttggg aaaatgcggc agcaattttt gcacgagttt cctctccgcc
     4741 actcattgtg tcgttttgta cattggaaac accaaggcga gataacattg cccggtcttc
     4801 gaccgtttct atttcgattc cgcccagttg gctgatatgt gcaaaatcac caaaacgctg
     4861 taatgtcgct tcggctaaaa caatttcgcc attaagtact ttgagtaaac tactctttcc
     4921 tgctccgtta tcacccacaa gaccaatacg gtcataagag tgaatttcca attcatcaat
     4981 atccaaaaca tcacgcccag cataatccaa gcgtatgttt ctcgctttaa taattaaact
     5041 catttttatt tactcctgtt tagctcttga aattttttat gcagcaaaca ggatttaggt
     5101 gaaaacaaaa gctagcatca cagtgtcctc ccaaaaaaaa agctattcat ccacagggtg
     5161 gacaaatagc tagtcaatta agttataact ggaaactatg cactaaaagc atacttataa
     5221 attaagcata cttttacttt atatatccgc atatattctt aaagacacaa caaaagccca
     5281 ccattataaa atagtgtcac tatgcaaata gttgtgtctt aacgaatgcg gatagaatgc
     5341 ataaacttac ctaaaaaata aaattcagtt ttatgataac tttactgtta aaagaagtct
     5401 agacaacctt gttttatgct tggatataag gcttatttta aaataatagt agtgaagatt
     5461 ttctaaaatt aacataatac accttatacg aaatgcttga tatttctctt taaatatcat
     5521 tatattaacg taagccgttc tggagtatag ggcaccagaa cggcttttta ttgattattc
     5581 atgttccacg atcactaatt gagcgatgtt ccacggtttt attcagtttt aataaacatc
     5641 atccggtttt ttgaggagtc attatttcaa gtgcttgcac tgaaattggc tcttcaaaaa
     5701 actggagtct tggctaacca tatggaagga aaaagcggct taggaagcct ctcttcttct
     5761 gttctggttc aacatgctcc gggataggaa tacgcttatt gtcttgctga ggagtagtca
     5821 attcgtcata gttcgttgct agttccgacc gaggatccgt tgctatatcc tgtttaaagc
     5881 tctgatcggt aaaggtagtc atattggctt ttggtgcttc tagtaagcgc tgcatagctt
     5941 caatctgttc ttgataaaac gattcacgtt ctaaagattg attttctctc tgtattgcct
     6001 gatttaattg tttttccagt atgtcaactt gacgttttag taagtcaact tctgtcaagt
     6061 tttgactgtt aattgattga ctttgattga ctgtatcctc ttttttttgt ggttccccaa
     6121 aaactcttag ggcctctgaa aagtcaatta atccatcaga tcctttagat aaatttcctt
     6181 tgtttatatg tgcatatatg gcttgtcttg aatatccata aagcttagct aactctgaaa
     6241 ctgacagttt tttcattatg taaaccagtt ttcaacttgt gttaatacct gactgttaac
     6301 ttaacaattt caattaacaa tgtcattgaa atcctaattt tctgagtaaa ggaagtaatt
     6361 ccttaaattt ctctgcatct tgcagcatgg cagctatacg tacagcaaac tgctgatagc
     6421 tttctgtgcc ttgtgaatat ttactcatct caggaagctc tgagagcttg ttggcgaata
     6481 gatggcgttg tttatctgtt atttttgaaa agaggtctaa tgtattcgga tctcttttag
     6541 attcgactga atgcgtggct gattttttct gtttaaagct aaatgaaaaa ccagtaatag
     6601 atctacctgt tttatgctgt tcaactttga caataatatc ggtatgttca tttacttgtt
     6661 ttaatgcaat gtctaaaaca tattttttaa aatcatacat tcgtttgtat tcagtctcga
     6721 gtacacctat tttttgtcta aaatcataca tagttatgag aggcgttttt ccagtactac
     6781 gccatgcaat caatatttca tataaacgaa cagcataagc acttgttaaa ttacttattt
     6841 gttgtatttc atactttgta aattgttctt ctaacctagt aattaaaggc acaatagcag
     6901 gggcaaaaat aagtctaacg acagcttcat tatcaatata agccacctcg ctcacccatc
     6961 ttgatttgtg attaatagtg ttaccttttt cactaagact ctgataactg aattgtcttg
     7021 caaacaagtc atcgcaagca tcttttaacg cctgataagc cgtatttcga tgtacaccaa
     7081 actgattaat ataactttca gcatgaactg ttagtggatc attagcattt atccctttac
     7141 ccgattccct tgcttcaaca atagctagga gaattaacct ctgttcaact agatcgaggt
     7201 tataactggc attgattaag gcgttatctt taacaactag ttctgttttc atatgaaaag
     7261 attatatttt taattcaata agacgaggtt atataattat ctcgcttaat gcaaggtaaa
     7321 cctcgtttta atgcaaggta aacctcgttt taatgcaagg taaacctcgt tttaatgcaa
     7381 ggtaaacctc gttttaatat ctctagaacc attatgaata aaggattaca gcttaattaa
     7441 aagcatttaa aagcttaaaa ataattaaaa gcccagggag taaaaaattg cccttggttt
     7501 tcgcttcgct caaactctat tgaatctcgc tttcgctcga ttcgtcgggg caatttttgg
     7561 ttcatatttt cgtgaatttg agaaaaagca aaagcaagat caaaagcagc tcggaattcg
     7621 cttcgctcat gagctttttt tctcgctacg ctcggtttag gaatcaaatt tggaaagttc
     7681 gcacccattc gggatgctct gtaaagcagg tgattgatgg atagggagct tgtaatcagg
     7741 gaaagggatg ggcaaatttt ctcagttcgc tcgtagacac tcgctctgtt tagcgactca
     7801 tgccacgaga tttcatttta ggcgcatgtt gctgtgcttt gagctgttgt tcctgtctga
     7861 gtttcatctg ttgttctgct ttgatctcat ccacacattt tttaatgact tgataggatg
     7921 ggtatagagt ctggtattgc ttggctttgg ctggatgttg ttgttggtac tgcttccagg
     7981 catgttcttt gaatttgtca ttttccaata aatccttcac accatgtttt ttctggtgtt
     8041 catgctgacc tttcagcttt ttgtgctcct ggtaaatcgc atcacgttgg gcttcccagg
     8101 ctttttttcc aaacagtaag ggtttgttct gagttaattc gttgtgttga tcgaccaggc
     8161 tttgcaattt gtcatgactt tgcttgagtc cgcttttcac gatctcctgg gcaagctgct
     8221 gattgaactc attttgatgt ttgtggtgct tggtcagggt tatttcggct tgtttgagat
     8281 tacgctgcat gaaatccagg tctagattcg cgtcttttgc gctgatttcg cgttttagca
     8341 ctttgccaag ggtttccctc tgtttttcct caaacagggc tttttcgtca attggtggcg
     8401 ttttagcgct atttttgatt tggatctgct gttctgtttt cagtgtgctg agtttgatct
     8461 cattttcagc aatcagcaga tccatatttt tgctgtattg cagctgagcc tgatttctct
     8521 gtttgatttc gtcattgctt cggctgattt cggtctcaat cccttttcgg cgcagttcgg
     8581 tgactttcgg cccttcgtgc agcgtggctt ctaaaccatt ctcctggtct ttgtagctgc
     8641 ggtggtcgac tcgggcagtg tatccagcca gatccaggtg catattgcag atatcggcaa
     8701 agtgttcgcg ccagtgttcg atttctccac tgtgtttctg atccagttcc cgggttttct
     8761 cggtgaagcc ttcctgagta agtttgcgtg tgctcatcag gatatgcgcg tgataattgc
     8821 gttcatcact accagatcca gtatgcggtg catgaataca ggcatcgacg gctacctggt
     8881 gtctgtccac gatactggcg catagctctt cgagcatggc caggcgttgt tcctgattta
     8941 attcacttgg aaaagcgatt tcaaattctc gggcaactgt cgagtttttt cgggtttccc
     9001 gttgttcaac ttcgttccag agtctttccc ggttttttaa atgttctggt gcgccttcgg
     9061 gtaaataaat ttctttatat tccacacctg tttttcggct gtaatcatgt tctcggccct
     9121 cacgttcaca atagatcttt tcacctgccc gataggcaat cgcagcagta gcggaacgcc
     9181 ctgctgatcg tgctacggtt ttgactgaaa agtggtaaat cgccatgcct attttcgctt
     9241 ttaaattttc acgtagtgaa gtacaaccgt ggatacggtt gtcggggtat tggggagacc
     9301 ccaatcgtac ttgcaaacga agtttgcata agtgcgctct tcgaggaaaa cattactata
     9361 catatagcac cttgaatgca tattcaagtg cgcttatgtt agggatggtt tcccaggatg
     9421 atggtattgg attaatgagc gttactgaaa cactagacag caaaatcaaa gcacaggaag
     9481 aaaagctaaa gcagttaaag gctcaaagac aggcagcact ggcaagagaa cgtgctaaag
     9541 agaaagaaca agctcgaaag gatgatacca gacgcaaaat tttaattgga tcctgtatgc
     9601 taaagatcac tgaagatgat gaacaagcca gagctaaatt aattgctcag atggataagt
     9661 atttaacaga tgagcgtgac cgaaagcttt ttaatttata aattttgagg gataagtagt
     9721 ggaagatcca acacttaata agttaatacg taatacagaa ttattcttcg aacatcactg
     9781 gaatgtagac ctattaggaa agcctcctga atggtcacca gtacatttta attttggaaa
     9841 aattcctaat tacgataaac aaggtgtata tgcgtttgtc aaaggggatt tagtgactta
     9901 tatcggtgtt gggacttcta gaggtgctgg ccgttatcgt ggtaatggct taagcgccag
     9961 ggtaatgaaa tattgccgat ggaatgaaga taaaactgaa tatattgcga tagatccaag
    10021 actgaaagat gctggatcca tcattacaat aggctttgag cgtgatacgg cttatttagc
    10081 aaatgcccta gaagtttttc ttatctctaa aatccaacct gtccacaatg tggttaaagt
    10141 tggtatgtaa tttcgcataa gagattttat gttaaatttt ttatatcaaa atttcaaagt
    10201 gtactgatga gtacactttg aatgggttct aacacccgaa tttcgtatat gagattttat
    10261 gttaaatttt ttatatcaaa atttcaaagt gtactgatga gtacactttg aatgggttct
    10321 aacacccgaa tttcgtatat gagattttat gttaaatttt tggtggtgtt tcaaaaagta
    10381 tgctggcatt aaacgaatcc attattgata taaaagaaca ctaaatcaaa ggctttgaaa
    10441 tgattttcaa gctttttgga gaagttgcag cttctcctaa agcctcgcct gattcgatgc
    10501 cgtaatcgac aattattgcc ttctataccc acagtgaaaa atttaccaat caactgtttg
    10561 cattctttaa aggtcgtaac aaagctatcc caatggtcac ttgatataca tgtatagctt
    10621 acacctaact gcttcaattt taatttaagt cgttttgcag tggctaaatc tcggttaccc
    10681 catacataag caacaatttc acctgtttct cgatgatagg cataaattag ccattgttta
    10741 ttttgcttat gaccaacaaa agtccaaaac tcatctactt caagggtttc ataatgactt
    10801 tgctgtgctc gaagttgata ttttgattgg ctaagtgtcc gtagaacttt accgatactt
    10861 actctttcaa tttctgctat atccctgaca ccactgcctc gcaccatgag gtgtaatatt
    10921 ttggttttga tacctgagtg acagccttga taactgagag catgatcacc aataaattga
    10981 cgtctgcata ttttacattg gtaattttgc ttaccatcta cttttgtgcc atttttcttt
    11041 atactgtcac ttaggcaggc tggacacttg atttctagag ttattcgcat ttctctattt
    11101 tatcaaaatc caacctgctt tttttcagca tactttttga aacaccacca caactgttaa
    11161 tgctgaaaat agagcttata ctcaacttat tcgtccataa tatataaatt cacaatattt
    11221 aaaagctggc tcatttcagt aaaagtcgca agatttttca ttttcacaag gctttccttt
    11281 agaataggcg catcttattt cttagctttg atttctcaat gattcagtta gaccagttat
    11341 ctatacgtcg cggtggacgt gttttatttc aaaaagcgtc gatgcagtta cacccaggat
    11401 ggaaaattgg cctaaccggt gtcaatggtg ccgggaaatc gaccctgttt tcagctttgc
    11461 ttggtggcat ggagtcagac accggttcgt tgacccgtcc tgcggtatgg accgtggcgc
    11521 atatggcgca ggaaatcaaa gcgctgaata tgaaagccat tgatttcgtc ttgtccggcg
    11581 atgaagaata ctgggatatt caacagaaac tggagcagcc agatcagctg gatgatgcag
    11641 aactggccca tttatatggc cgttttgatg aaatttcagg ttattctgcg ccggccaaag
    11701 cttcgcaact gatggccggt ctgggctttt ttgaacatca atcccaactg gatgtttcaa
    11761 gtttttctgg tggctggcgc atgcgcctga atctggcgcg taccctgatg agccgttccg
    11821 a
//
