LOCUS       NZ_CP044467            12109 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter schindleri strain HZE23-1 plasmid pHZE23-1-4, complete
            sequence.
ACCESSION   NZ_CP044467
VERSION     NZ_CP044467.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12478518
            Assembly: GCF_010918635.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter schindleri
  ORGANISM  Acinetobacter schindleri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Non-baumannii Acinetobacter spp. carrying blaNDM-1 isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) College of Veterinary Medicine, South China
            Agricultural University, 483 Wushan Street, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:25:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 28 of 96
            Pseudo Genes (incomplete)         :: 72 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 23 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044467.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Acinetobacter schindleri"
                     /mol_type="genomic DNA"
                     /strain="HZE23-1"
                     /isolation_source="stool"
                     /host="goose"
                     /db_xref="taxon:108981"
                     /plasmid="pHZE23-1-4"
                     /geo_loc_name="China:Huizhou City, Guangdong Province"
                     /lat_lon="23.12 N 114.42 E"
                     /collection_date="2018-01-20"
                     /collected_by="chaoyuecui"
     gene            1..951
                     /gene="repM"
                     /locus_tag="FSC10_RS16030"
                     /old_locus_tag="FSC10_16145"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="FSC10_RS16030"
                     /old_locus_tag="FSC10_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_163172873.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKKGRSISGFS
                     FTFKQKKKDNPSIERDPNTLDLFSKMTDAQRHIFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDIVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="FSC10_RS16035"
                     /old_locus_tag="FSC10_16150"
     CDS             944..1519
                     /locus_tag="FSC10_RS16035"
                     /old_locus_tag="FSC10_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_163172875.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQTSQQIESSTSDSDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQDNDNKKNNELNIVENVAVPQQETTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDHD"
                     /name="plasmid replication DNA-binding protein"
     gene            1940..2284
                     /locus_tag="FSC10_RS16040"
                     /old_locus_tag="FSC10_16155"
     CDS             1940..2284
                     /locus_tag="FSC10_RS16040"
                     /old_locus_tag="FSC10_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146112.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="WP_005146112.1"
                     /translation="MNSVLLAYVLLGFAIVSEVIGSSFLVKSEGFTKLFPSVMVFIFFS
                     TAFYLLSQVIKTIPLGIAYAIWAGVGIVLTAVVGYFVFKQTLDNPALIGIGFIVSGVII
                     INLFSQSAGH"
                     /name="SMR family transporter"
     gene            2285..2842
                     /locus_tag="FSC10_RS16045"
                     /old_locus_tag="FSC10_16160"
     CDS             2285..2842
                     /locus_tag="FSC10_RS16045"
                     /old_locus_tag="FSC10_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_005146114.1"
                     /translation="MKEIRQQKKQPEVIRKRILEQATILVSQKGISGVSIQNIATAVGI
                     TKGGVFHHFPNKKNLIEEMFNQIILDLDQTVEYLIKRDPTEYGKFTRAYIHSSFIHQID
                     GVVSAWSALSMTMITEPTFNKIWMEWLKNRLSQHAETDSHPDLELLRLAADGLWLQSIT
                     GVIDDEQCSKLEDDLLNRTYLT"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2965..3669
                     /locus_tag="FSC10_RS16050"
                     /old_locus_tag="FSC10_16165"
     CDS             2965..3669
                     /locus_tag="FSC10_RS16050"
                     /old_locus_tag="FSC10_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067784.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_005028420.1"
                     /translation="MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHT
                     TIYRWVQRYAPEMEKRLRWYWRNPTDRHLWHLDETYVKVNGKWAYLYRAVDQRGHTLDF
                     YLSARRNTHSAYCFLGKILNNVKKWQIPQVINTDKAPTYGRALSRLKREGKCPPDLEHR
                     QIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIKGIEVMRALRKGQASSFYYGQPQ
                     GEVCLINRVFGL"
                     /name="IS6 family transposase"
     gene            complement(<3723..3971)
                     /locus_tag="FSC10_RS16055"
                     /old_locus_tag="FSC10_16170"
                     /pseudo=""
     CDS             complement(<3723..3971)
                     /locus_tag="FSC10_RS16055"
                     /old_locus_tag="FSC10_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253717.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(3999..5213)
                     /gene="floR"
                     /locus_tag="FSC10_RS16060"
                     /old_locus_tag="FSC10_16175"
     ARG             complement(4003..5213)
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5430..>5561)
                     /locus_tag="FSC10_RS16065"
                     /old_locus_tag="FSC10_16180"
                     /pseudo=""
     CDS             complement(5430..>5561)
                     /locus_tag="FSC10_RS16065"
                     /old_locus_tag="FSC10_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /name="DUF3363 domain-containing protein"
     gene            complement(<5560..6589)
                     /locus_tag="FSC10_RS16070"
                     /old_locus_tag="FSC10_16185"
                     /pseudo=""
     CDS             complement(<5560..6589)
                     /locus_tag="FSC10_RS16070"
                     /old_locus_tag="FSC10_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612089.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     misc_feature    complement(6184..6300)
                     /locus_tag="FSC10_RS16070"
                     /old_locus_tag="FSC10_16185"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo=""
                     /db_xref="RFAM:RF01497"
     gene            complement(6772..7338)
                     /locus_tag="FSC10_RS16075"
                     /old_locus_tag="FSC10_16195"
     CDS             complement(6772..7338)
                     /locus_tag="FSC10_RS16075"
                     /old_locus_tag="FSC10_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581699.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_006581699.1"
                     /translation="MPNQVGYIRVSSTEQNTERQLDGISLDKTFTEKASGGSRERPQLN
                     AMLDYLREGDTVHVHSIDRLARNTNDLNEIVNSLNDRGITIIFHKENLTFSHDVAQSAI
                     NKLMFQMLAAFAEFERSMIRERQKEGIAKAKAKGLYKGRKRKVDYVEIRKAMAEENSTF
                     RGVAEKFKVGIATVQRALKEETNNQ"
                     /name="recombinase family protein"
     gene            complement(7516..7779)
                     /locus_tag="FSC10_RS16080"
                     /old_locus_tag="FSC10_16200"
     CDS             complement(7516..7779)
                     /locus_tag="FSC10_RS16080"
                     /old_locus_tag="FSC10_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127801456.1"
                     /translation="MSTVDLIDEDDNESGIAAKAANVLRDRFIAAAQRGTVLYVENDNL
                     MSKTPNGVPILVKHLDGRNPDLAYRLAGRRTFKIKKRKINSN"
                     /name="hypothetical protein"
     gene            8281..8604
                     /locus_tag="FSC10_RS16085"
                     /old_locus_tag="FSC10_16205"
     CDS             8281..8604
                     /locus_tag="FSC10_RS16085"
                     /old_locus_tag="FSC10_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104442387.1"
                     /translation="MNPITFQKLEKMNKHCKACVKAYALLTLLSLVTFFIYFFNRFTLE
                     ILVTDTTALFPVIKMEALALGIMHIVYCFFFKYVDKKLQIFGLDSNNLNEYIQRNQAFF
                     QKY"
                     /name="hypothetical protein"
     gene            complement(8601..8894)
                     /locus_tag="FSC10_RS16090"
                     /old_locus_tag="FSC10_16210"
     CDS             complement(8601..8894)
                     /locus_tag="FSC10_RS16090"
                     /old_locus_tag="FSC10_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="WP_080548207.1"
                     /translation="MQKYPDDYYLSRQEIDDLRAEAKHAAKYMKAFFDFKTEARYLFSV
                     TWSAQDGEFIGLCAEFPSLSWLDPDRDKARSGIERLVFDVLQDMSSTGEAIP"
                     /name="antitoxin HicB"
     gene            complement(8937..9929)
                     /locus_tag="FSC10_RS16095"
                     /old_locus_tag="FSC10_16215"
     CDS             complement(8937..9929)
                     /locus_tag="FSC10_RS16095"
                     /old_locus_tag="FSC10_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227554861.1"
                     /translation="MIYYSYVPKDFTKHVMGMMTNEYDLVAKKFILNMNTDEASRMISK
                     WIERYHLLETAQQTYRRRLNSEPVFSLLVHFTYSYLPGLSESECWEKFDKNEPAFLVQV
                     EAYLFCRTSDAFLLDEKTQKVLSKTDKQDLLKINRKIFEICPSSESFSYIGEVNPISCG
                     RYELVRLTKPKKSIKELQAKNWTNEKHVTDWTWRLTDKAYKEQLEQGKRVVLRFQSLIE
                     KNASLDEKKAYFERHFRALEGYLGYRGVRQQIGNLYHLEKRLFKDKYNQPWFDHGARTL
                     KLSYMKKLKSSIVNNSSYQEAEAHFRSVLTEDLNKKYEKWKAKSNKTEV"
                     /name="hypothetical protein"
     ARG             1941..2284
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="46.23"
                     /coverage="106.54"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.425"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa agacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa cccgactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa aaggtcgatc aatttcaggt
      661 ttttctttta cgtttaaaca gaagaaaaag gataatccat caatagaaag agatccgaat
      721 accttagacc ttttttcaaa gatgaccgat gctcaacggc atatttttgc aaacaaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacatag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttacagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaacat ctcaacaaat tgaatcttct acatcagata gcgatgaatc
     1141 tacaaaaaaa ctcattattc atttagaaaa tgaagttaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caagacaatg ataataaaaa gaataatgaa ttaaatattg tagaaaacgt
     1381 ggcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaaatgtg gcagtgccac aacacaaaaa acgtggatta tttggccgtg tgctaaatgc
     1501 tgtttttgat catgactgag caatctaggg agagaaatca ttccgaaact gaaagacatt
     1561 gccctgggaa ttattgtggc cccgctgctg atcccgatca tgctgattgc atcgtaccaa
     1621 gataaaaagg cactcaaaaa agagttggat gaacgccaaa aagaaaaaga ccaggcatcc
     1681 tgattattca attgagctaa tcaaaagtgt ggaacattgc tcacttatta agcgtggaac
     1741 gtgaaaagtc ataaaaaaag cccaaacagt gatgtttggg ctataaactt tgaaataagt
     1801 atttgaattt caaaataaat tataacacat ttcgtataag gtgtattatg ttaattttag
     1861 aaaatctaaa ataattattg ataatttttt tttgaaaatt taacatacct tccgattggt
     1921 attttaggta tgattactta tgaacagtgt gttactcgct tatgtattac ttggatttgc
     1981 aatagtttcc gaagtgattg gaagtagttt tttagttaaa tcggaagggt ttacaaaact
     2041 ttttccaagc gtgatggtct ttattttttt tagcacggca ttttatttat tatcacaagt
     2101 tattaaaaca atccctttag gtatcgcata cgctatatgg gctggtgttg ggattgttct
     2161 aactgctgta gttggctact ttgtttttaa acagacacta gataatcctg ctttaattgg
     2221 aattggtttt atcgtatctg gcgtaataat tattaattta ttttcgcaat cagcaggtca
     2281 ttaagtgaaa gaaattcggc aacaaaagaa gcaaccagag gttattcgaa agcgcatcct
     2341 tgaacaggca actattcttg tttctcaaaa gggtatttct ggtgtttcta ttcaaaatat
     2401 tgctacagca gtaggtataa caaaaggggg ggtatttcac catttcccga ataaaaaaaa
     2461 tttgatagaa gaaatgttca atcaaataat tcttgattta gatcaaacag ttgaatactt
     2521 gattaaacgc gatccaacag aatatggtaa attcacacga gcttatattc atagttcttt
     2581 tatccatcaa atagatgggg tggtttctgc ttggtcagcg ttatcaatga ctatgatcac
     2641 agagcctacc tttaataaaa tttggatgga atggctaaaa aatagattat ctcaacatgc
     2701 agaaacggat agtcatcctg atttagagtt acttcgttta gcagcagatg ggttgtggct
     2761 acaaagtatt accggagtca ttgatgatga gcaatgttca aaattagaag acgatctgct
     2821 taatcgaacc tatttaacat aaaatctctt attcgaaatt tgaactgtaa gccccagata
     2881 gaaaaatcca gtgcatagaa cggcactgtt gcaaataggc tgacatgata agctaaatat
     2941 cttatttatt tcgagataca gcagatgaat cccttccacg gtcggcactt tcaaggtgaa
     3001 atcattcttt gggctgttcg ttggtattgt aaatatggca tcagctatcg tgaacttcag
     3061 gaaatgctgg ccgaacgtgg agtgaatgtt gatcacacca caatttatcg ctgggttcag
     3121 cgttatgctc cagaaatgga aaaacgttta cgttggtatt ggcgtaatcc tacagatcga
     3181 catttatggc atctggatga aacttacgta aaagtgaatg gcaaatgggc atatctatac
     3241 cgtgcagttg accaacgtgg ccatactctt gatttctatc tttctgctag acgaaatacc
     3301 cattcagctt attgttttct tggaaaaatt ttaaataatg tgaagaagtg gcaaattcca
     3361 caagtgatca acacggataa agcacccaca tatggacgtg ctttatcacg gttaaaacgg
     3421 gaaggtaaat gtccaccaga ccttgagcac aggcagatta agtataaaaa taacgtgatt
     3481 gaatgtgatc atggcaagct aaagcggatc atcagggcca cattaggatt caaatctatg
     3541 aagacggctt atgccacaat taaaggtatt gaagtcatgc gtgcactacg taaaggacaa
     3601 gcatcgtcat tttattatgg tcagcctcag ggtgaagtgt gtctaatcaa cagggttttc
     3661 ggtctctaag tactttttaa agggaacatc atcgactcaa atctctattt gcaacagtgc
     3721 cccatgaagt gcctgcctgc gtctgttagc cgaacgcccc gcgcatggcg ctcaaatagc
     3781 aggacaccaa ggttatcctc cagcgctttc acacgcgcgc tgacgctcga ctagctgata
     3841 ccaagtgcct tggccgcatg ccgaaaattc agatgctcgg cgacggcgat gaactgaaca
     3901 agggaaatga gcggtatcct gccagacagg ataccgacat tcacgaggtt tcgatggtta
     3961 gtgcgccgca tcggagcggg cctgctacca gtcgtcggtt agacgactgg cgacttctcg
     4021 gtggcagccc cacggagccg aaggagcacc agccccaacg aaaccagtac cgccatcgcc
     4081 gtggcgtaac agatcacggg ccacgctgtg tcaccgttta aaagtgccac cgccaatgtc
     4141 ccgacaatgc tgactatcag gctttgaacg cagaagtaga acgcgaccgc tgatcccgcg
     4201 atgtcgtcga actctgccaa agcgccgttc gcggtaacgg acaccgtgaa gacaataccg
     4261 accgcgacaa cccacatcgg taggatgaag gtgaggaatg acggcgagcc gtaaagttcg
     4321 ccgatcccca acaggaccgc tccgcaaaca agcaacgcca tcccacgcgc cacgcatcct
     4381 gcgatgcccc atctggcgac aaaggacttc gcgaaacggg ttgtcacgat cattacaagc
     4441 gcgacagtgg cgaaggcaaa gctgaatccg atctcggaat attccgcttg gcctatgagc
     4501 acacggggag ccgtcgagaa gaagacgaag taggtgccca taccggcgct aaagccgaca
     4561 gtgtaaaccc aaaaagccgg actcgcgaag atcggcaaga cagatcggcg cgtcttgact
     4621 tgatccagag ggcgggtttc gtgccacctg aaacccgcat ttaggagtgc gagcatcgcc
     4681 agtatagcca aagtaatgaa tatcgcctgc catcccaaga actcgccgat caatactccg
     4741 gcgatagggc cgagcgcagg cacgaacgcc agcatcgaac tgaaaaggcc gtagatgacg
     4801 acaccctcag gacggttggc ataaacgtcg cgaaccgtcg cgaacgtcgc caccagcatg
     4861 gccgacgcgc ccactgcttg aagtagacgg aaagcgacaa aggccggtgc agttgaagac
     4921 caagctgctc ccagagacgc aatgacgaaa gccgttgcgc ccgcaagtag aattggccgt
     4981 cgcccgattc tgtctgagag cggaccaaaa atcacctggc ccacgccgag catcaccata
     5041 tagaggctca acgtgagttg gatcatagcg ggcgtcgtgt tcaggatgcc gggcatcgct
     5101 ggaacgacag ggagataaat atccatcgcc agtgaagcga ggatgtcgaa aggagccatc
     5161 agcagcagtg ctgccggcag cgtataggcc cacgcggggc gtgtggtggt catgacgaat
     5221 caaccctcga ttaaggaata ccgggcgacg tctgctcgtc agcaatcaga tgagactagc
     5281 cttacagagc gccgcaacaa caatactggt tgttgcggct tacttgtctg ctgacttgga
     5341 atttcccatc tgattactcc acgcttacga atatgaattg ctacatttta tccgattatc
     5401 ttttgcttcg caatgcggca tgggcgcact cagcgttcca gccccctctg ccgccctaac
     5461 tgccacgaca ccgacccgcc ctgcacgatg cccataacct cgcggccgag ttggcggtcg
     5521 atgatcggcc gccacgggac aagggtgaac tcatgggatt tcagccaaag cattatcgta
     5581 tgaatcacca gtcgtaccga ctgatgctcg taaatttgct gcttctaaac gattggtata
     5641 gcgaatggaa agatattgca cacctctgtc ggaatgatga atcacattct ttggcatgcc
     5701 tcgatcatgc aatgcttgct ccaatgcatc gagcaccata tctgtattca tccgtgtcga
     5761 tactttccat ccaacaattg ctcgtgagaa cacatcaata ataaatgcag tataaaccca
     5821 gcctgaattt gtttgaatat acgtaaagtc actcacccac agttggtcag gatgatcagc
     5881 agtaaaatta cgttttacca agtcatccgc tcgtttttga tcatctcggc tacgggtggt
     5941 ttgtttgttc ttaccacgcc aaacaccttt tatacctagc ttttgcatta atcgagcaac
     6001 agtacagcgc gcaataatat agccttcacg tttcagtttt tgccagactt tacgtacacc
     6061 ataccgacct gaactttcct tccaaattcg tttaatctcc tcagcatgat gcaagtcatg
     6121 taaatctcgc tttgctcgat gttctggatt ttcacagaga tctagagtcc ggtaataggt
     6181 tgaaggtgcg atcggtaaaa ttctacaaat cgcctcgact ccgtacagct ctttattatt
     6241 atggataaaa tccaccatta tttgtgtggg cggtcgagct ccgcctgggc gaaaaaagcg
     6301 gctgctttac gtagaatttc attggcgcgt tgcagttctt tattttcgcg ttcgagttgt
     6361 ttgatacgtt cttggtctga aagctgctgt actttaactg gattttgttt atctaaatat
     6421 ttttgatacc aaacacgtag tgtttcagga gtacaaccct tgcgcagtat actgctcagg
     6481 gagcaatagc ggtgatcgca gcccaattcg atggataatc tttctcggat tcaatcaata
     6541 attgaaccgc tctttctctg atttcagggg tatattttac ttttttcatc gggacattct
     6601 ctcagaaagc ttggtctccg acaaacccgg tacggttcaa aaatcttcca ttgatttatt
     6661 tatatctgtt aaaccagtcg cattatgaaa tgtaatataa gtagaaattt cagtataaat
     6721 ggtcatgatc ttttctaaat ttgaatatct aagcgtcttc attaatcaac cttattgatt
     6781 attagtttct tcttttaaag ctctttgtac tgttgctatc cctactttaa acttttctgc
     6841 aacacctcta aatgttgaat tttcttcagc cattgctttt cgtatttcaa catagtcaac
     6901 cttccgcttt cttcccttat ataagccctt cgctttggct tttgcaattc cttccttttg
     6961 acgctcacgg atcatggagc gttcaaactc tgcaaatgca gcaagcatct gaaacatgag
     7021 tttatttatt gccgattgtg ctacatcatg actgaaggtc aaattctctt tatgaaaaat
     7081 aatggttata ccacgatcat tcagactatt cacaatttcg ttcagatcat ttgtattacg
     7141 tgccagacga tcaatactat gaacatgcac cgtatcacct tcacgcagat agtcgagcat
     7201 tgcatttaac tgaggacgtt cacggctacc accgcttgcc ttttcagtaa aggtcttatc
     7261 caggctgatt ccatctaatt gacgttcagt attttgttca gttgaactga ctcgaatata
     7321 tccgacctga ttaggcattc tgtaccgtta gagtttagat aattaaatac atattgtacc
     7381 gaaattaaat attaagtcta taaatacggt ttttgagttg ctttttaaga ctttattttg
     7441 ttccgttagg ctatactcta aatttacaga acatctctta ttcgaaattc gatcgaatgg
     7501 gagtaaaagc ctgcctcaat tcgaattaat tttacgtttt ttaattttaa aagttcgtcg
     7561 cccagcaagt cggtaagcaa ggtcaggatt ccggccgtcc aagtgtttaa ccaaaattgg
     7621 cacaccattc ggtgttttac tcattaaatt atcattttct acatacagca ctgttcctct
     7681 ttgcgctgcg gcaataaagc ggtctcgaag tacatttgca gctttggcag caatacctga
     7741 ttcattgtca tcttcatcaa ttagatctac tgttgacata gtgccgtact cccaattagc
     7801 tgatatatga atcatattac aagtcgggta gacaggataa agaactagag acctttctac
     7861 atataatgct ctgatcgaca cacacaatag cttctttcca catcaaggta ggtattatgt
     7921 gtgatctgaa ttttatattt aatttcaata acttataaat attaatgttt gagttttatt
     7981 tttgccacat aaacaaaata atatttaaaa tcaaatgctt atattttata aaaaatttaa
     8041 agcttagcat cacacatgat agctacctaa attagctcca ttaggtagct atcatgtgtg
     8101 tcgatattaa aaattatgtt taatttcaat gtattaatag aattcaactc tttataattt
     8161 tgatattttt tttacaaagc acgggatcat ctaacagaaa ttttttccct tttttaatcc
     8221 tggatattta gaatcggtag aaatttcttc acttaaaaat ttaactgcct agagagcaaa
     8281 atgaatccta taacttttca gaaactggaa aaaatgaata agcactgcaa ggcttgtgtt
     8341 aaagcctatg cacttttgac tttgttgagt ctggtgactt ttttcatcta tttttttaat
     8401 cgtttcacat tagaaattct cgttacggac acgactgctc tatttccggt tatcaaaatg
     8461 gaagcgttgg cacttgggat aatgcacatt gtttactgct tcttttttaa atatgtcgat
     8521 aaaaaacttc aaatattcgg cttagattca aataatctga atgaatacat ccagcggaat
     8581 caggcatttt tccaaaaata ttaaggtatg gcttctccag tggaactcat gtcttgtaat
     8641 acatcaaaga caagtctctc aatgcctgag cgtgctttat cacggtctgg atctagccag
     8701 gatagcgacg ggaattcggc acagagtcca ataaattccc cgtcttgggc ggaccatgtc
     8761 acactaaata aataacgagc ttcggtctta aaatcgaaaa aagctttcat atattttgcc
     8821 gcgtgtttag cctcagctct caaatcgtca atttcttggc ggctgagata ataatcatca
     8881 ggatatttct gcatgagtta tcaccattgt taattgagat tgatttaaaa aacatcctat
     8941 acctcggttt tattggactt cgccttccat ttttcgtatt ttttgttgag atcttccgtt
     9001 aatacactcc taaaatgagc ttctgcttct tgatatgatg aattatttac aatcgaactt
     9061 ttcagttttt tcatatatga aagttttaaa gtccgggcac catgatcaaa ccagggctga
     9121 ttatatttat ccttgaataa gcgtttttct aggtgataca aatttccgat ctgctgacgt
     9181 accccacggt agcctaaata gccctccaag gcccggaaat ggcgttcaaa ataggctttc
     9241 ttctcatcca gactggcatt tttttcaatc aggctctgaa accgcaaaac gacccttttc
     9301 ccttgttcta gttgttcctt ataggcttta tcagtcaaac gccaggtcca atctgtgaca
     9361 tgtttttcat tggtccaatt cttggcttga agctctttaa tgcttttctt cggcttggtc
     9421 agacgtacca gttcatatct accacagctg attggattaa cctcaccaat gtaactaaaa
     9481 gactctgaac ttggacagat ctcaaagatt ttacggttaa tcttcaataa atcttgtttg
     9541 tctgttttgc tcaaaacttt ttgagttttt tcatccaata agaaagcatc actggttcga
     9601 caaaacaggt aagcctctac ttgcaccaaa aaagcaggtt catttttatc aaatttttcc
     9661 cagcattcac tctcactcag tcctggtaaa taactataag tgaaatgaac cagcaagcta
     9721 aaaactggct cactgttcaa tcttcttcga taggtttgtt gggctgtttc cagtaaatga
     9781 taacgctcaa tccattttga aatcatacgg cttgcttcat cagtattcat attcaatatg
     9841 aatttcttag caacaagatc atattcgttc gtcatcattc ccatgacatg ttttgtaaaa
     9901 tctttaggaa catagctgta ataaatcatt tttttattac tcttaaagtg attttaataa
     9961 agtgattcta taagagattt ataaaagata tttattaaaa attctataag aaattcttaa
    10021 agtctcaaaa atgcaatttt tgagacttta agcatgattg cgaatcacct tcggagattc
    10081 tataaatagc agttccccaa aatgggcttg acgaccgcaa cttgatcaaa tataaaactt
    10141 ttagggtctt tgattttttg cgtggactgc caatccatga gccaattctt ttctgtttgg
    10201 ttcctgggtt gcctacccat gaatcagaca cactccccct tcttgaggat tgccagtctt
    10261 tgagaagctt tagtggtgaa gctgcgtgcg tgaggaaact ctagctggcg cggctacact
    10321 aggggctggc gggtgtgact cccgctttct acctgttcac tcattttgat gaatgaacac
    10381 cctccactcc caaggtggag gtcagttggg cgacaaggac acgggatcac ggtgacgtgg
    10441 cggtgggagt aacttccctc ctaggctgaa agggccaaaa ccctgtatta aaacagacag
    10501 tcagaggact cacggaatga gtaacctcgt gaatagctga taaccagacg cttcaagtca
    10561 aaaacaaaaa taaagacaag ggcgtaggtt atacccacag gtgggctttt gagggcatcg
    10621 tgaggtgtca gggttgcaag gcgaaagctg gcgcaatcgg tactcgattt taggtagaac
    10681 agtgaatttg tcggaagaca ttctgttttg ctgtcgagat caatggatag ctcaagcctg
    10741 taaggtcgtc aacatggtgc tttttaggct gtgtatgtcg atatgggtaa ggcgtgaacg
    10801 tggatgatta ttaactacat attgcattgt ataaacctga tacgacttgg aactgcgatc
    10861 gggggagata ggaatgtagg tgttgtatgt aggagatcgg cacgggttct gccagttttc
    10921 aggcaactga aaacaactgt tttaatacta acttgggaag cagttttgtt taatgacgaa
    10981 gccgtattgc ttaaagattt ctctttaatt tgaagaggtc tttattcaat cggaggcaac
    11041 ttccgaaaac agaactgtgg gagagtgact tagtagggct ttgctgtaat ggcaaaggaa
    11101 gcatttcaat tcgagtgaaa ggcgagcgtt gaaatgagta acgaaaggac acgccattaa
    11161 gttggagtgt gtaagagaat tggaagaatg tggtttgagg atgaagtttg gcaacttcat
    11221 agacttggcg gtctaaaccc tccagccaca tttgtaaaaa tcgacctaga agatcagatt
    11281 cgagtggcat cggatccaga aattccaaga accttgagaa aacaagtcaa atcaaagtct
    11341 ttgatccgac tttttaaaaa cggttagtgg ggacctcact taaaaaatcc tgacgcagcc
    11401 gattctgctg aacgaaatta aaaatctgtg tgtccctcat cacacataaa taactgagat
    11461 caaagtcctg agtacgacta taaactgctt tccattttgc gctgatttgc gttttaagcc
    11521 attttctcta tttgatatag gtaatcttga taatcagtga aaaaacgcgt ctacccccta
    11581 ttttttgcct ctaatcgtta tatcagagcg agtgtctacg agcgaacatc aaaaagtcgt
    11641 gcctagactc cctgaaaaac agctttttag gctgtgaagt caaaaaaggc gtaaatcata
    11701 tgtagaaaat tgagcctaga ccgagcgaag cgagcaaaaa agtactatga gcgaagcgaa
    11761 ttccgagttg cttttgcttt ttctaaaact cacgaaaata ttaaccaaaa aattgcccct
    11821 cgaatcgagc gaaagcgaga ttcaatagag tttgagcgaa gcgaaaacca agggcaattt
    11881 ttcattccct tggcttttaa ttattttaaa gtttttaaat gcttttaaat ggcttgaaag
    11941 ccttatgaat aaagggtttt agctcttata tgtccacgtt taccttgcaa tatgtccacg
    12001 tttaccttgc aatatgtcca cgtttacctt gcaatatgtc cacgtttacc ttgcattagt
    12061 acacaaataa tattaacgtg tacttataca caataaaaaa atagtggct
//
