LOCUS       NZ_CP044466            13172 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter schindleri strain HZE23-1 plasmid pHZE23-1-3, complete
            sequence.
ACCESSION   NZ_CP044466
VERSION     NZ_CP044466.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12478518
            Assembly: GCF_010918635.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter schindleri
  ORGANISM  Acinetobacter schindleri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Non-baumannii Acinetobacter spp. carrying blaNDM-1 isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   Cui,C., Chen,C., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) College of Veterinary Medicine, South China
            Agricultural University, 483 Wushan Street, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:25:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,281
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 28 of 96
            Pseudo Genes (incomplete)         :: 72 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 23 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044466.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Acinetobacter schindleri"
                     /mol_type="genomic DNA"
                     /strain="HZE23-1"
                     /isolation_source="stool"
                     /host="goose"
                     /db_xref="taxon:108981"
                     /plasmid="pHZE23-1-3"
                     /geo_loc_name="China:Huizhou City, Guangdong Province"
                     /lat_lon="23.12 N 114.42 E"
                     /collection_date="2018-01-20"
                     /collected_by="chaoyuecui"
     gene            <3..296
                     /locus_tag="FSC10_RS16455"
                     /old_locus_tag="FSC10_16040"
                     /pseudo=""
     CDS             <3..296
                     /locus_tag="FSC10_RS16455"
                     /old_locus_tag="FSC10_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(316..>459)
                     /locus_tag="FSC10_RS15935"
                     /old_locus_tag="FSC10_16045"
                     /pseudo=""
     CDS             complement(316..>459)
                     /locus_tag="FSC10_RS15935"
                     /old_locus_tag="FSC10_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(515..829)
                     /locus_tag="FSC10_RS15940"
                     /old_locus_tag="FSC10_16050"
     CDS             complement(515..829)
                     /locus_tag="FSC10_RS15940"
                     /old_locus_tag="FSC10_16050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127800359.1"
                     /translation="MTIIKNVTDFPENFFGFHPEDLSPDEKNRPLGKIFIKLQKLRNDL
                     GLKCILIPGFAFKSISIINIEQDEFQTLLQKFGPENYNNSSFGARSFIFFAPHHLLNQQ
                     "
                     /name="hypothetical protein"
     gene            complement(1053..>1618)
                     /locus_tag="FSC10_RS15945"
                     /old_locus_tag="FSC10_16055"
                     /pseudo=""
     CDS             complement(1053..>1618)
                     /locus_tag="FSC10_RS15945"
                     /old_locus_tag="FSC10_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742756.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            1661..2611
                     /locus_tag="FSC10_RS15950"
                     /old_locus_tag="FSC10_16060"
     CDS             1661..2611
                     /locus_tag="FSC10_RS15950"
                     /old_locus_tag="FSC10_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635327.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="WP_163172865.1"
                     /translation="MGQVLHSSATTTQAVRRAIQNSQESLRTLAKRYGINQKTVAKWKK
                     RTSVDDLTTGPKNPRSSVLSPEDEAAIVAFRKRTLLPLDDCLYALQPSIPYLKRSSLHR
                     CLQRHGISRLPEVQGDRPVRKRFKSYPIGYFHIDIAEMRTAQGKLYLFVAIDRTSKFAF
                     TELHTKASKMAAAEFLRNLVKAVPYRIHTVLTDNGIQFTNRTCDRYASEHIFDRICTEC
                     GIEHRLTKVKHPWTNGQVERMNRTIKEATVKRFHYDDHTQLCIHLADFIAAYNFGRRLK
                     TLRGLTPYEFICKQWTDEPELFKIDPIHQMPGLNT"
                     /name="IS481 family transposase"
     gene            complement(2963..3265)
                     /locus_tag="FSC10_RS15955"
                     /old_locus_tag="FSC10_16065"
     CDS             complement(2963..3265)
                     /locus_tag="FSC10_RS15955"
                     /old_locus_tag="FSC10_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004830812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="WP_127800881.1"
                     /translation="MKIIQNLSLILAAVALTACANNPEQMPSQQPNTMTSCITDEATKL
                     VGQTGLSEAVIKAKTKAEIVRMVAPGQPVTLDYRSNRVTVVTDPQTKIIVQVSCG"
                     /name="I78 family peptidase inhibitor"
     gene            complement(3485..3787)
                     /locus_tag="FSC10_RS15960"
                     /old_locus_tag="FSC10_16070"
     CDS             complement(3485..3787)
                     /locus_tag="FSC10_RS15960"
                     /old_locus_tag="FSC10_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="WP_127800883.1"
                     /translation="MKIIYHVSLIIAAVTLTACAGTPNTDVVQKPEMMNKCVPESAAAL
                     ARTTLSSEADIKSKTNAKVVRLVAPGQPITKDYHPGRVTVIADPQTKTILKAFCG"
                     /name="I78 family peptidase inhibitor"
     gene            4103..>5100
                     /locus_tag="FSC10_RS15970"
                     /old_locus_tag="FSC10_16080"
                     /pseudo=""
     CDS             4103..>5100
                     /locus_tag="FSC10_RS15970"
                     /old_locus_tag="FSC10_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611389.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(5120..6210)
                     /locus_tag="FSC10_RS15975"
                     /old_locus_tag="FSC10_16085"
     CDS             complement(join(5120..5670,5670..6210))
                     /locus_tag="FSC10_RS15975"
                     /old_locus_tag="FSC10_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="WP_085940648.1"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKL
                     YISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
                     KKLIAVLAISFCWCYLTGEWQHNQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFG
                     KKEEFKEILAILRKQNPDRIRVL"
                     /name="IS4-like element ISAba1 family transposase"
     gene            6300..7115
                     /gene="sul2"
                     /locus_tag="FSC10_RS15980"
                     /old_locus_tag="FSC10_16090"
     ARG             6301..7112
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7202..>7444
                     /gene="glmM"
                     /locus_tag="FSC10_RS15985"
                     /old_locus_tag="FSC10_16095"
                     /pseudo=""
     CDS             7202..>7444
                     /gene="glmM"
                     /locus_tag="FSC10_RS15985"
                     /old_locus_tag="FSC10_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(7680..>8510)
                     /locus_tag="FSC10_RS15995"
                     /old_locus_tag="FSC10_16105"
                     /pseudo=""
     CDS             complement(7680..>8510)
                     /locus_tag="FSC10_RS15995"
                     /old_locus_tag="FSC10_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            8574..9278
                     /locus_tag="FSC10_RS16000"
                     /old_locus_tag="FSC10_16110"
     CDS             8574..9278
                     /locus_tag="FSC10_RS16000"
                     /old_locus_tag="FSC10_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067784.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1006 family transposase"
                     /protein_id="WP_001067784.1"
                     /translation="MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHT
                     TIYRWVQRYAPEIEKRLRWYWRNPTDLSSWHIDETYVKVNGRWSYLYRAVDQRGDTIDF
                     YLSSRRNTKSAYCFLGKILNNVKKWQIPQVINTDKAPTYGRALSRLKREGKCPPDLEHR
                     QIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIKGIEVMRALRKGQASSFYYGQPQ
                     GEVCLINRVFGL"
                     /name="IS6-like element IS1006 family transposase"
     gene            complement(9306..9773)
                     /locus_tag="FSC10_RS16005"
                     /old_locus_tag="FSC10_16115"
     CDS             complement(9306..9773)
                     /locus_tag="FSC10_RS16005"
                     /old_locus_tag="FSC10_16115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176773.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="WP_228722247.1"
                     /translation="MSKSALLVIDLQNEYLPTGKLPLVNIEQAAANAVKVIAKARKQGT
                     QVIHVQHIADAESPIFEPSSNGIEFQDTVKPQEDESVIIKNHINAFLNTNLKEILDSNQ
                     VTELVVIGAMSHMCVDAAVRAASDFGYKVKVIHDACATLDLEFKALLQIEI"
                     /name="cysteine hydrolase family protein"
     gene            complement(9858..10742)
                     /locus_tag="FSC10_RS16010"
                     /old_locus_tag="FSC10_16120"
     CDS             complement(9858..10742)
                     /locus_tag="FSC10_RS16010"
                     /old_locus_tag="FSC10_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010326910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_163172867.1"
                     /translation="MNKILSSSVIALSLAMASVHLYANDKVVQRDTSKVTHIQEIRNAT
                     IKVTYADTTFLIDPMFAKKGFYEGFPDTHRSYLRNPLVDLPIKPETILEGVDAVIVTHT
                     HLDHWDDAAQATIPKNMPLFVQNKEDQKIIQSQGFKDVRVLTQATFEGIKLTKTGGQHG
                     TDAMYRIPKLKAGLGEAMGVVFEAAGHETVYVAGDTIWRSEVDQAIQKFKPDVIVLNTG
                     NALVDGFKESIIMGKEDTYRATQKAPNAKVVAVHMDAINHMSVTRAELAEYVKDKGIQD
                     KVLIPIDGETLSF"
                     /name="MBL fold metallo-hydrolase"
     gene            10875..11846
                     /locus_tag="FSC10_RS16015"
                     /old_locus_tag="FSC10_16125"
     CDS             10875..11846
                     /locus_tag="FSC10_RS16015"
                     /old_locus_tag="FSC10_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733159.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_163172869.1"
                     /translation="MAIPVIGLFVYPNINPFHFSIPHIIFNIRIEDKRLFDLKIFSIDG
                     QPVKTEQSMMIIPDGGIELIKESDLIIIPGWDDFNHEPEEVLINALNCAYQTGIRIVGL
                     CYGTYALAYAGLLDHKKAATHWMAEQDFSERFPQVQLDNDALYVEDQRIVTSAGTGAAL
                     DCCLYLVREIYNARIANKVSRVMVIPPHREGGQAQFIEQPVAESSQDAQINVLLDFLRK
                     NLALQHNIESLAERTHMTRRTFTRHFKKATGMTLVDWLNTERIRLSYELLETTNLSIEK
                     ITELSGFNNTESFRKNFREKYGTSPQAWRKAFGIINENNPPI"
                     /name="helix-turn-helix domain-containing protein"
     gene            12373..12729
                     /locus_tag="FSC10_RS16020"
                     /old_locus_tag="FSC10_16135"
     CDS             12373..12729
                     /locus_tag="FSC10_RS16020"
                     /old_locus_tag="FSC10_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004668307.1"
                     /translation="MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAIVLCIADKGGKKRFYKDMLDIADEQ
                     YQLHLTTLGDKSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            12722..13024
                     /locus_tag="FSC10_RS16025"
                     /old_locus_tag="FSC10_16140"
     CDS             12722..13024
                     /locus_tag="FSC10_RS16025"
                     /old_locus_tag="FSC10_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140620.1"
                     /translation="MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(<13066..>13170)
                     /locus_tag="FSC10_RS16615"
                     /old_locus_tag="FSC10_16035"
                     /pseudo=""
     CDS             complement(<13066..>13170)
                     /locus_tag="FSC10_RS16615"
                     /old_locus_tag="FSC10_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472779.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
ORIGIN
        1 cggataaagc gcccacatat ggacgtgctt tatcacggtt aaaatgggaa ggtaaatgtc
       61 caccagacct tgagcacagg cagattaagt ataaaaataa cgtgattgaa tgtgatcatg
      121 gtaagctaaa gcggatcatc agggccacgt taggattcaa atctatgaag acggcttatg
      181 ccacaattaa aggtattgaa gtcatgcgtg cactacgtaa aggccaagct tcgtcatttt
      241 attatggtca gcctcagggt gaagtctatc tggtgaatag agttttcggt ctctaagcac
      301 tttttaaagg gaacatcatc gactcaaatt tctatttgca acagtgccct caggatttaa
      361 gtcagggcta tatttaggta gccatacaag gtaatgacct gctttctgaa gtaattcttg
      421 cagtctttta cctttatgga acgtcgcatt atctatgatt ggctgcttaa cctctgcttt
      481 taactttggt tatttatggg aggattaccc taagctattg ctggtttaat agatgatggg
      541 gagcaaaaaa gataaaactg cgtgcgccaa aagaagaatt attgtaattt tctggaccaa
      601 acttttgaag caaagtttga aattcatctt gttcaatatt gataatcgaa attgatttaa
      661 atgcgaatcc tggtatgaga atacatttta ggccaagatc atttctaagc ttctgtaatt
      721 ttatgaaaat ttttcctaga ggtctatttt tttcatctgg acttaagtcc tcagggtgaa
      781 agccaaaaaa attttctgga aagtcagtaa cgttcttgat aatagtcatt aatttcagag
      841 taataattcg ttaaataaaa ctttaattca aaaattaaaa aagctcaagc tattgttttt
      901 tatcaacata aaacattgac cgaaaaatat tagagttaat tgtccaaaat tatatttcta
      961 ttttggattt acacccgatg ttgttgttgt tattcccaag ggctttgttg cacaaagatt
     1021 tgaaatgcaa agcgccccta attgagccaa atttaaggcg taacttgggt aagtggtcga
     1081 cctaattccg taaatctgtt gaggactgct acacgtgcat ggatctcatt cacttggcta
     1141 tcaaaactcc ttgcactgag tttatctcct aataatttga tgcaatgcat cttagtttca
     1201 accaacttcg ccgatgatag cctgaccatt ttttccatag tgtcctgcct aaacgtttaa
     1261 ttgttcgaag taattcattt cgctctagcg agctactctt tgtatctttc catggtttcg
     1321 cattttttct aggtggaatc accgcatgtg cttgccgatc tgcaatgacc tgacggcatt
     1381 gcttggtgtc ataagctcca tcggtataaa cagagtcaat ctgctcatct tgtggaatct
     1441 gattaagtaa atcaccaagc acctgtgaat cactgacatt attggttgtg agctgaatag
     1501 cgcgtatttg tagggttttg gcatctatac caatatgtag tttacgccat tggcgacgat
     1561 attcagctcc atgtttcttg cgtttccatt tgccctcacc taggtgttta gtcccagatt
     1621 ttaatggtgc aatattctca gataaatgga aggaagcact atgggacaag ttctacacag
     1681 cagcgccaca acgacacagg cagtgcgtcg agcaatacaa aatagtcaag agagcttaag
     1741 aacgctagcc aagcgttacg gcatcaatca aaagaccgtc gcaaaatgga agaaacgtac
     1801 ttcagtagat gatcttacaa ccgggccaaa gaatcctcgc tcaagcgtgt tatcgcctga
     1861 agatgaggcg gcaattgttg cttttcgaaa acgcactttg ttgccgctag acgattgcct
     1921 ttatgcattg cagccgagta ttccttatct gaaacgctcg tcgttacacc gctgcttgca
     1981 gcggcatggt atttcacgac tgcctgaggt acaaggtgat cgccctgtca ggaagcgctt
     2041 caagagctac cccattgggt actttcatat cgacatcgct gagatgcgaa cagcacaggg
     2101 taagctttat ctcttcgttg ccattgatcg cacttccaag ttcgcgttca ctgaattgca
     2161 tactaaagcc agtaagatgg cagccgcgga gttcctgcgc aatctggtta aggctgtgcc
     2221 ctatagaatc catacggtgc taacagataa cggcatccag ttcacgaacc gcacctgtga
     2281 tcgctacgcc tctgagcaca tctttgatcg gatttgtact gaatgtggca ttgaacatcg
     2341 tcttaccaag gtcaagcatc catggaccaa tggacaggtc gagaggatga accgaacgat
     2401 taaagaagct actgtcaaac ggttccatta cgacgatcac actcagctat gcattcacct
     2461 tgctgatttt attgctgcat acaacttcgg tcgcagactc aaaacactca ggggcctgac
     2521 tccctacgaa ttcatctgca aacagtggac agatgaacct gaacttttta agattgatcc
     2581 gatccatcaa atgccgggac taaacaccta gctttactgg tgactaaatt ataagtgctc
     2641 ccattcttca ccattataac catcaagcag tcaccctgcc gatcttcttc atgcaaacac
     2701 tgcatgatga caatagtcgg tgtgtccggt gagttagtac gagactcaag cgtattctgg
     2761 agctacttga ttaccctcgc cttccttaat gggacaactt ctttcaaaca atcccgacaa
     2821 accttgattc ctccatcatt aattatgtaa tcgatttcag tcataccatg caggccgaat
     2881 aaacagcata ggaattgaga cattactctt ttccaaataa taaaaaacca tccgaagatg
     2941 gtttttgttt agtctttagc tattagccgc aggaaacttg aacgataatt ttagtttgtg
     3001 gatcggtgac taccgtaact ctatttgagc ggtaatctaa agttactggt tgacctggcg
     3061 caaccattcg tacaatttca gctttagtct tagctttaat tactgcttca gataaaccag
     3121 tttgtcccac aagctttgtt gcttcatcag ttatacagct agtcatcgta tttggttgtt
     3181 gagaaggcat ctgttcagga ttatttgcac atgctgtcaa tgcaactgcc gctagaatta
     3241 aagataaatt ctggataatt ttcataagct tttgtcttat caggattaaa aaggtaacca
     3301 taccaaatac gataacctcg actgtaggct taactacaat ctctatacat tctatttaaa
     3361 ccgaatattt aataagaaaa gtattgctct cttaaagtta aggtttaaaa aattccatac
     3421 agatttaatg ctcacaacta aaaacctccc gaaggaggtt ttgtacacta tagcatttaa
     3481 gtttttaacc acaaaatgcc ttaagaatag ttttagtttg tggatcagca attactgtta
     3541 ctcgtcctgg atgataatct ttagttatag gttgaccagg tgcaacaagt cttacgacct
     3601 tagcatttgt ttttgatttg atgtcagctt cactcgatag tgttgtacgg gctaatgctg
     3661 ctgcactttc aggtacgcat ttgttcatca tttcaggctt ctgaacaacg tctgtattag
     3721 gagtgccggc acaagcagtc aaagtcactg ctgcaataat aagcgataca tgataaataa
     3781 ttttcataag cttctatctt taacatataa aagcaggatc atacctagga aggcttagag
     3841 cagcctagtc atatcaacaa tgttaataca cttctttaaa tattgagatt taaaaaaacc
     3901 acttaactct cccaagttaa gcaggcacat gtcaggtcat caatcttttt tgctcttttt
     3961 ctccacaagc atcctaacaa ataacttatc tagccaaata gtgaatagcc actaattttg
     4021 tagacacctt ttagttagat atggactgcc accactctcc tagacaaatt tcgtctattc
     4081 tttttgcata ggagagaaca ttatgagtgg acaacgatac accccagaat ttaaagaaga
     4141 agctgttaaa ttgattactg aacgtggata ttctgtcact gatgtggcag aacgtttagg
     4201 tgtatcacag cacagcattt ataaatggct aaaggccgta cagcctttac gcaacaaccc
     4261 tgatgaacat gaactactcg aagcaaagaa agagatcctt cgtcttaaaa gtcagcttaa
     4321 gcaaactgaa gaagagcggg atatcttaaa aaaggccgca aggtactttg caagcctgcc
     4381 cgagtaaagt atcagtttat ccttgagtac agccatcaat ttaagattaa gacgatgtgt
     4441 cgggttctta agattgctcg tgctggctat tatgcctggc tacatgaacc cgaatcaggc
     4501 agaacaattg aagacaaacg gttattacaa ctgatccgtt cttcttatga tgccagttat
     4561 ggtatctatg gttatcgtcg catcacgctg gatcttaaag agctaggtga aagctgtgga
     4621 ccgaatcgtg tgctgaagat catgaagaac aatggcattg ccgctgttcg aggatataag
     4681 aagcataaaa gctatggtcg tggtcgtcct cagattgtgc cacctaacca tttaaatcga
     4741 gaatttgttg taaacacacc tgatacctcg tgggtgactg atattaccta catccgcact
     4801 tggcaaggct ggttatatct ggctgtggtt ctcgatttat attcaaggaa agtcatcggt
     4861 tggtcaatga aacctacgct tgccaaggat atcgttttgg atgccctttt aatggcggta
     4921 tggcgacgca gacccaatga acctgtgatc atacactcag accaaggctc acaatacagt
     4981 agcggagact ggcagaaatt ctgtcagaag cataatttag ttccgagtat gagtcgtcgt
     5041 ggaaactgtt gggacaatgc tgttgctgaa tcctttttta gcagcttaaa gaaggaaaga
     5101 actctgtaca cgacaaattt cacagaaccc ttatcctatc aggattctgc tttcttaaaa
     5161 ttgccaaaat ttccttaaac tcttcttttt tcccaaaacc aattaaacgc tgaatcgcca
     5221 tttgaacata gtctaaacca tagcgaaata aactcattga gagtcgtcca tgcttcttta
     5281 tttttatcgc ttttttttga ttatgttgcc attcacccgt taagtaacac caacagaagc
     5341 ttatagctaa caccgcaatc aattttttca ctcgtctagg gtctgtcaag cgcgtatttt
     5401 caagattaaa cccgcgtcct ttgagacaac tgaataaggt ttcaatttcc cagcgtaatg
     5461 cataatcctg aatagcattg gcattaaact gaggagaaac gacgagtaaa agctctccat
     5521 tttctaactg tagtgcactt atatatagtt tcacccgacc aaccaaaatc cgtcgtttac
     5581 gacattcaat ttgaccaact ttaagatggc gaaataaatc actaatttta tgattctttc
     5641 ctaaatgatt ggtgacaatg aagttttttt aacacgaatg cagaagttga tgtcttgttc
     5701 aattaaccat gtaaaccact gctcaccgat aaactctctg tctgcgaaca cattcacaat
     5761 acggtcttta ccaaaaatgg ctataaagcg ttgaatcaaa gcaatacgct ctttcgtatc
     5821 tgaatttcca cgtttattaa gcaatgtcca aaggataggt atcgctattc cacgataaac
     5881 gattgcgagc atcaggatat taatatttcg ttttccccat ttccaattgg ttctatctaa
     5941 agtcagttgc acttggtcga atgaaaacat attgaaaatc aactgagaaa tttgacgata
     6001 atcaaaatac tgacctgcaa agaagcgctg catacgtcga taaaatgatt gtggtaaaca
     6061 cttgatgggc aaggctttag atgcagaaga aagattacat gtttgcttta aaataatcac
     6121 aagcatgatg agcgcaaagc actttaaatg tgacttgttc cattttagag atttgtttaa
     6181 gataagatat aactcattga gatgtgtcat agtattcgtc gttagaaaac aattattgtg
     6241 acattatttc aatgagttat ctatttttgt cgtgtacaga gattattata gaagccccca
     6301 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
     6361 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
     6421 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
     6481 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
     6541 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     6601 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     6661 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     6721 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     6781 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     6841 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     6901 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     6961 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     7021 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     7081 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     7141 attcgcggtt cagcaggcat gtcccctttg agggcgaccc gacgacagga taatcgacct
     7201 tatggtgcgc aaatatttcg gcacagacgg tattcgtggc aaagccaacg aaggcgcgat
     7261 gacggcggaa accgccttgc gcgtcggcat ggcggctggc cgtgtctttc gtcgcggtga
     7321 ccaccgccat cgtgtcgtga tcggcaagga tacgcgcctg tcgggctata tgcttgaacc
     7381 cgcgctcaca gccggtttca cctcgatggg catggacgta ttcctttttg gcccgctgcc
     7441 gacaacgtat aggaagaata aacgcccttt tcacccaagt ccaacagctt tggaccgcag
     7501 ttgactcttt cgacacccct gcgatgcaac ccaatccggc tgacggggag ccagcaacgc
     7561 tgaaaattta ccctcctctt tcccactagc ggctcctttt ccgacaacca gcacggcgga
     7621 tccctgccgc ggcgctgtga acgcagcatt ttgattggta tcgttggcct tcaggctcgt
     7681 cagtcaaaca gacccaggag cagctcagcc ggtggcgccc ggctttcggg taacgccctg
     7741 gtcccgctgg tttcggcttt gtgcttcagg tgatcaagga tctgcttgat cactataggg
     7801 tcttcaatgc aggcgatgac tttcatggcg ccgccgcagc cgctgcaggt ctcgatgtcg
     7861 atattgaaaa cacgcttgag ccgttgcgcc catgtcatcg acgctcgccg ttgtgctggt
     7921 gttgccggtt catcagccac cctgaccttg ttgcccctgc cccgttttgc cggcgtgacc
     7981 aacgcccggt gccgactgtt gggtgcgaac accccgtgga agcgggttag gttgactctg
     8041 ggcttcggta ccagggcggc cagccttgca atgaaatcca atggttcgaa aatgacgtgc
     8101 gtggtgccgt cccggtacgg cgtcttgagc tggtagcgca cgttgccgcc tcgtgttaac
     8161 gacagccgct tctcggatac cgccgggcgg ctgatgtacc ggcacagccg ttcgagcttc
     8221 ttgcgttcat cggccctggc cgccacgccg gcgtgcaggc tggacccggc taccttgcca
     8281 atcccgtcac cgaacggatc accactggtc ggcagagttt gcaaagtgaa cacctttcgc
     8341 cccgcctgtg aaccgacagc gatacggtaa gtgatcgagt gccccagcag gggtgtcatc
     8401 gggtcgtcat ccaccgcatc cgaggccaga tagctgtttt cgacatcccg ttccagcagg
     8461 ccttgccgtt ccagatagcg acccacccgg tgggcgatgg tgtgcgtcag cggcactgtt
     8521 gcaaataacc acgaatggta agctgaagac tgttttagct aaaggtgcag catatgaatc
     8581 ctttccatgg tcggcatttt cagggcgaaa tcattctttg ggctgtgcgc tggtattgta
     8641 aatatggcat tagctatcgt gaactgcagg aaatgctggc cgaacggggt gtgaatgttg
     8701 atcacacgac tatttaccgt tgggttcaac gttatgctcc tgaaatagaa aaacgtttac
     8761 gctggtattg gcgtaatcct acagatctga gctcgtggca tattgatgaa acctatgtaa
     8821 aagtgaatgg acgatggtct tatctgtatc gtgcagtcga tcaacgtggc gataccattg
     8881 atttttatct ttcttctaga cgtaatacca aatcagcata ttgttttctt ggaaaaattt
     8941 taaataatgt gaagaagtgg caaattccac aagtgatcaa cacggataaa gcacccacat
     9001 atggacgtgc tttatcacgg ttaaaacggg aaggtaaatg tccaccagac cttgagcaca
     9061 ggcagattaa gtataaaaat aacgtgattg aatgtgatca tggcaagcta aagcggatca
     9121 tcagggccac attaggattc aaatctatga agacggctta tgccacaatt aaaggtattg
     9181 aagtcatgcg tgcactacgt aaaggacaag catcgtcatt ttattatggt cagcctcagg
     9241 gtgaagtgtg tctaatcaac agggttttcg gtctctaagt actttttaaa gggaacatca
     9301 tcgactcaaa tctctatttg caacagtgcc ttaaattcca agtctagtgt tgcacaagca
     9361 tcatgaataa cttttacctt ataaccaaaa tcagaagcag cacgaacagc agcatcaaca
     9421 cacatatggc tcatagcacc aatcacgact aactcagtca cttgattgct atctaaaatt
     9481 tctttgagat tcgtgtttaa gaacgcattg atgtgatttt taatgatcac agattcatct
     9541 tcttgcggct taactgtgtc ctgaaattca ataccatttg agctaggttc aaatatagga
     9601 gattcagcat cagcaatatg ttggacatga atcacttgag taccctgttt acgggccttc
     9661 gcaattactt tcactgcatt agcagcagct tgttcaatat ttaccaatgg taatttacct
     9721 gtaggtagat attcattttg taagtcgata actaataaag ctgattttga catatctatt
     9781 cctttactta actaatattt gttttgttct gcttaggatt ttaaagaagt ggaagtattc
     9841 cacttcttta agtttcttta gaaagaaagc gtttcaccat caataggaat taaaacctta
     9901 tcttgaatcc ctttgtcttt tacgtactct gcaagctcag cacgtgttac tgacatgtgg
     9961 ttaattgcat ccatgtgaac agcaaccact ttagcatttg gtgctttctg agttgcacga
    10021 taagtatctt ctttgcccat aataatggat tctttaaagc catcaaccaa ggcgttacct
    10081 gtatttaaga caatcacatc aggtttaaat ttttgaatcg cttgatcaac ttcagaacgc
    10141 cagatggtat ctcctgccac ataaacagtt tcatggccag cagcttcaaa taccacaccc
    10201 attgcttcac ctaatccagc tttaagctta ggaatacggt acatggcatc agtaccgtgt
    10261 tgaccaccag ttttagtgag cttaatccct tcaaaggtcg cttgagttaa tacacgaaca
    10321 tctttgaagc cttgtgactg aatgattttc tggtcttctt tattctgtac aaacaacggc
    10381 atgtttttcg gaatagtcgc ttgtgctgca tcatcccaat gatctaaatg ggtatgggtc
    10441 acgattaccg catctacacc ttctaaaatc gtttcgggct tgataggcaa atcgactaat
    10501 gggttccgta aatagctacg atgagtatca ggaaaacctt catagaatcc ttttttagca
    10561 aacattgggt caattaaaaa agtcgtatca gcataggtta ctttaatcgt tgcattgcga
    10621 atttcttgta tatgcgtaac tttagaagta tctcgctgaa ccaccttatc attcgcatat
    10681 aaatggactg aagccattgc cagagagagt gcaataacag agctacttaa aattttattc
    10741 atgttccaga tccaatcatt tagtcatact cgaatcatga taggaaaaat taagcaataa
    10801 aattgtcttt aaggacaatc atcgaaacta ttgggacaac atgctatatt tattatttta
    10861 gagaggtaga ccgaatggct ataccagtga ttggattgtt cgtttatccc aatataaatc
    10921 catttcactt ctctatcccc catatcattt tcaatatcag gattgaagat aaacgcttat
    10981 ttgatctgaa aattttttct atagatggcc aacccgtaaa aacagaacag tctatgatga
    11041 ttatccctga tggggggatc gaactcatta aagagtcgga tcttattatt attcctggat
    11101 gggatgattt taatcatgag cctgaagaag tacttatcaa tgccctaaat tgtgcatatc
    11161 agactggaat tagaattgta gggttatgct atggaactta tgcattggca tatgcagggc
    11221 tgcttgacca taaaaaagca gctacgcact ggatggctga acaagatttt agtgaacgat
    11281 ttccacaggt tcagcttgat aatgacgccc tgtatgtaga ggaccaaagg atcgtaacct
    11341 ctgctggaac aggcgcagcg ttagactgct gcttgtactt ggtacgtgaa atctataatg
    11401 cacgaattgc gaataaagtc tctagagtaa tggtgatccc gcctcaccgt gaaggtgggc
    11461 aagctcagtt tattgaacag ccagtcgcag aatcgagcca agatgcacaa attaatgtat
    11521 tacttgattt tttaagaaag aatttagctt tacaacataa tattgaaagc ctcgctgaac
    11581 ggacacatat gacacgcagg acgttcacac ggcattttaa gaaagcgaca ggcatgaccc
    11641 tagtcgattg gctgaatact gaaagaatca gattaagtta tgaactttta gaaacgacaa
    11701 acttgtctat cgaaaagata accgagcttt caggttttaa taataccgag tcatttagaa
    11761 aaaattttcg tgaaaaatat ggcaccagcc ctcaggcatg gagaaaagct tttgggatca
    11821 ttaatgaaaa taatccaccc atttaacata atggcggtta tacgaagtac acttgtatat
    11881 taatataaat atcaaccact taataaaaca agaaaaatcc aagaatgctc aaaaatacga
    11941 ttttgaaaca agtcggcttt tttatgagcg gttttgatag ttcttgccaa taattttgtt
    12001 taaaaagtag tcgattttgc gattttttaa attttatcaa tgcgtgaatg ttcaaaatta
    12061 tccccatttc ttgtggataa atttcaggca ctttgtacgc tttagcttac tcaaattcca
    12121 gttctttgcg gctatcagga tagctagaat atccctacga tagattcata caggaacagg
    12181 atgttccgga gcaaaaaaca acaatgataa gttctaacac taggattgtg aggtagagca
    12241 gcacctacca aagatcgcac aataaaaaac cccggcagga taccggggtt ttttattgcc
    12301 tatcctttct catttgcaaa acaaacataa tgcccctaaa atatagacta taatctatat
    12361 tctggttgtg gtatgtggac agtcattacg acagatctat ttaatcagtg gcttgaacag
    12421 caagatgaac ctacacaaga gaaggtccta gctgccctgg ttgttctaca gcagcaggga
    12481 ccgagtttag gccgtccttt agtagatact gtgtatgagt ctaaatttac caatatgaaa
    12541 gaactgcgtg ttcaacatcg cggtagaccc ttaagagctt tcttcgcatt tgatccctta
    12601 cgacaggcta ttgttctatg tattgctgat aaaggcggta aaaagcgttt ttataaagac
    12661 atgctggata ttgcagatga acaataccaa cttcatctca ctaccctagg agataaatct
    12721 aatggctaag actctgcaag aattgttagc tagccgctct ccagagagcc aagcccgtat
    12781 tcaaaaaatg gctgatgaat tactgctaga aaatcagctt catcttattc gtgaagaact
    12841 cgaaatttct caaaaagagc ttgctgaaac tttgggaata aaacagccat cgctttcagc
    12901 aatagaaaat cgtggcaatg atcttaagat ttcaaccatg aaaaagtatg ttgaggcaat
    12961 gggtgggaag ctccgtattg atgtagagct tccaacagga aaacatatag gattcaacgt
    13021 ttaacaataa aaatgggagc tataggctcc catttttaga ttcgtataac gttgaaccca
    13081 acggtaaata gtcgtgtgat caacattcgc accccgttcg gccagcattt cctgcagttc
    13141 acgatagcta atgccatatt tacaatacca gc
//
