LOCUS       NZ_CP044442             4665 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli strain HeN100 plasmid pHeN100_05, complete
            sequence.
ACCESSION   NZ_CP044442
VERSION     NZ_CP044442.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12868540
            Assembly: GCF_026427755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Peng,Z., Hu,Z., Li,Z., Li,X., Jia,C., Zhang,X., Wu,B., Chen,H. and
            Wang,A.X.
  TITLE     Characteristics of a Colistin-Resistant Escherichia coli ST695
            Harboring the Chromosomally-Encoded mcr-1 Gene
  JOURNAL   Microorganisms 7 (11), 558 (2019)
   PUBMED   31726755
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Peng,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2019) College of Veterinary Medicine, Huazhong
            Agricultural University, NO.1 Shizishan Street, Wuhan, Hubei 430070,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026427755.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 05:55:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,874
            CDSs (total)                      :: 4,752
            Genes (coding)                    :: 4,500
            CDSs (with protein)               :: 4,500
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 63 of 252
            Pseudo Genes (incomplete)         :: 195 of 252
            Pseudo Genes (internal stop)      :: 45 of 252
            Pseudo Genes (multiple problems)  :: 47 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044442.1.
            Bacteria and source DNA available from Xiangru Wang, College of
            Veterinary Medicine Huazhong Agricultural University, China.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HeN100"
                     /isolation_source="ENVO:00010483"
                     /db_xref="taxon:562"
                     /plasmid="pHeN100_05"
                     /geo_loc_name="China: Henan"
                     /lat_lon="38.98 N 77.11 E"
                     /collection_date="2018-10"
     gene            complement(join(3666..4665,1..500))
                     /locus_tag="HeN100_RS01735"
     CDS             complement(join(3666..4665,1..500))
                     /locus_tag="HeN100_RS01735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_112921167.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPSGQREKLMASFERVLMPGLDKDQYSILWVEHRDKGRLE
                     LNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
                     PKAKQEAAEAITCGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRH
                     PRPRPETVLSHEPAHERHGAHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHP
                     GAEEHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAA
                     PVVERLNAVELHREQERVVQHQKALELERSQRQRTYNSPSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(490..813)
                     /locus_tag="HeN100_RS01740"
                     /old_locus_tag="HeN100_01910"
     CDS             complement(490..813)
                     /locus_tag="HeN100_RS01740"
                     /old_locus_tag="HeN100_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1871..3427)
                     /locus_tag="HeN100_RS01745"
                     /old_locus_tag="HeN100_01925"
     CDS             complement(1871..3427)
                     /locus_tag="HeN100_RS01745"
                     /old_locus_tag="HeN100_01925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_226426401.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGARKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGAFFSSGNRYLLGYAHMKNPAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEMALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          complement(927..1099)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          1024..1576
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(7..500)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="99.0"
                     /evalue="1.93e-100"
     Typing          complement(497..813)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          complement(3670..4664)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgctttag gcagtgcaga cggtgtgacc agtgcccgcc ggttctccgg tgcgttcggg
       61 tcgtgcagcc ccagtctgcc gttcacgatg gtctgccagg catcgatgcg tggacggtcg
      121 gcgcgatcgt aatacggctg gagccgtctg ccggtcagca gctccgtgtt cgggatcaga
      181 aaattcagct caagccgccc cttgtcacga tgctccaccc acaggatgct gtactggtct
      241 ttatcaagtc cgggcatcag aacccgctca aagcttgcca tcagcttttc ccgctggccg
      301 gaaggtaaat ccgcttccgc aaacgacagt accccggagg tgtatttctt aacgtaaggc
      361 gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacggt ggcgccttca
      421 cgctgccggt ctttacccag cagatagtcc accggcccgg cgccaccgcc gcgaccgcgt
      481 ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc gcagccgctc
      541 aagtccggca tcgatggcca tcagcgccgc aacaatctgc accctgtcat ggccggtacc
      601 gccgccggcg ttaacacggc gggctatctg gttcaggtta ttgcccatgc cggcaagctg
      661 gcgcagcagc gccggcgaga ttgagggaag ctttccggaa cgtgccggct tttcgtccag
      721 gcaggtctgc cgcatccacg ctgccagctg cctgccgtcg cagcgctcaa gcagccgccg
      781 gtgctcgtcc tccgtcaccc acatggtgag catcttgttc cgtttctctg acaaaccggc
      841 ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg gttggacaag
      901 ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag ccctgaacca gtcacgtagc
      961 gctagcggag tgtatactgg cttactatgt tggcacggat gcgagtgtca gtgaagtgct
     1021 tcatgtggca ggagaaaaat gcagcagcgg agcgtcagca gaatatgtga tacaggatat
     1081 attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtac
     1141 cggcttacag gcggggcgga aatttcctgg gagatgccag gaagaagctt aacagggaag
     1201 cgataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     1261 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1321 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccgatgtcaa
     1381 tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcggttcc gggaaggcag
     1441 ttcgctccaa gctggactgt atgcacgaac cccccgttca gcccgactac cacgttgtcc
     1501 cggtaactat catcttgagt ccaacccgga acagacacga aaaatcgcca gtggcggtag
     1561 ccattagtaa gaggattcac ggagcggagt agtagagtgt tctacagagt tcttgaagtg
     1621 gtggcctgac tgcggctaca ctggaaggac agttttggta cctgcgctcc gtgaaagcca
     1681 gttaccttcg gaagaaagag ttggtagctc tgaattccgg caaacgaacc accgttggta
     1741 gcggtggttt ttttgtttac agggtatgag attacgacga catcgaaggg ataacaagag
     1801 gatccgcttc ttgcccactc ttcgccacaa aaaaaacgac acactagcgc gtaaagcatc
     1861 atcaaaaaac tcaaatagcg ctatatacaa tcactttatt acgcccctcc cttttggctt
     1921 gataaagagc atcgtcagca tttttcagca aaaaatcgat cctaccaaga ggatcatctt
     1981 gtgttgccac accagcagat atggtgaccc gcaagttatt agggaagaca gtactttcaa
     2041 taacactcct aatacgctcc gcaacacact tagctgttac cacagatgta ttcggcagga
     2101 aaacaacaaa ctcttcgcca ccaaaccgac aaacaacatc ttcactacgg caacaggaat
     2161 taataatatg agccaaagaa acaagcacct catctccgac cccatgcccg aaactgtcat
     2221 ttatctcttt aaaaaaatca acatcaatta caataactga ataccctgaa tcccgcttat
     2281 gattctgtat tttacttaaa aaccccaatc tattgttcac gccggtaagg ggatctttaa
     2341 gcgccatctc atttaaagaa tcaacccttt ccatcattac cttaacatgc gtaaaaagtg
     2401 cctttttaag tcgatcagct tcggcatacc aagcattgat agctttaata taaactaacg
     2461 aagcccctat atctttagca ttggtagata tagcaagctt ttctaaagga agtgaaatac
     2521 gggaggcaat gaaatatgaa agaagtgaaa atgtaataat tattacaatt agcagaacaa
     2581 gtacactttt agcataagta ataagaatag tagtcgcgct gtcagcatgg ctataaacca
     2641 acacattcca agcaggattt ttcatatgag cataacccag aagatatcta ttccccgaag
     2701 aaaaaaatgc cccctcacct ctatcagact ccgttaactt tgatttcaaa tcatcagaca
     2761 taaccatggg ccgaccaaca gcactgttat cgttattaaa gatcacagtt ccatcatcac
     2821 taacaatgct aatttcagtg tcgctcttga aaaaatgcct gctcaaaaca tcgctaaata
     2881 aactatgttt ttttagatat atagacccac cgacatgccc tatgtaattg ccattcttat
     2941 cataaatagg atgtgacaac aaaataatca aattcccagt aactgatttg aacggtgaag
     3001 atatgaatgg tttcttttcc tcaatcgcca atttgctggc acttgaattc aagcgggtac
     3061 caactaaatc aagagattct ggcgaagtcg ccagaacaac agcatcatta ctaacaacaa
     3121 aaactgaatt aaacatacca gactgcaatc ttaacctatc cgcctcatct ttaagtgagt
     3181 caatatcatt agttgatttt atttttcttg ctccatatgc taattcacct tgagccattt
     3241 caatataacc actcattaca tctgacattt tcatagcgta cgatgaatta gcataaagca
     3301 aatctttttc tatattttcg cgctgaaaaa ccataataac ggcaatcaga aaaatagaag
     3361 ttaacaaaat cccaccaacg gacaaggtta tgactatcgg acgaaggtga ggctttttaa
     3421 ttctcataaa aatacactcc atataataaa aaatgccgca tttaccataa catcccttat
     3481 cagcaccgcc ccggacgggt tcggaacaaa tgcggcgtca gcccggtaaa accggcagca
     3541 aactctccgc taaaccgcgc attctgcgcc agataaattt cacccggctg catggctatg
     3601 cagtcgggtg aatggctccg gctttcagcg cagatgtccg ccgaaagccc cgttttatca
     3661 ggttatcaca gtgaagggct gttatacgtc cgctgtcgct gagatcgctc cagctccagc
     3721 gctttctgat gctgcaccac gcgctcctgc tcccggtgca gctcgacggc attcagtcgc
     3781 tcgacaaccg gcgcagctgc tcgctcaaac tctgcacctg cctgctcaag cccgtgacgc
     3841 gctcgctcag cgctgcgttc tcccgttgca taagaccaga catcgtccgc cattccgcga
     3901 aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc
     3961 tcagcaactg tttttccagc tccgtcatgt gctatttccc cgccagtatc atccagctcg
     4021 aatcctctcc gaaccgtccc gcttccgggt tcaccttcac gcagcggcgt ctctgctcgc
     4081 cgcaggtcga gagctgcacg ccgctgttcc tctctgacag catggcctgc gtgctcttct
     4141 gctcccggat ggtcgtgtag ttgtcgagta tctgctgccc ctgccaccac agaatgcccg
     4201 cgctcgaggc gatcagcagc gcggagacca ggacgatggt cagccacgtc tggctgacca
     4261 tgcgcaccat gcctttcgtg cgctggctca tggctgagga cagtttccgg tcgtggtcga
     4321 ggatggcggc gctgattctc ttcgcgcttt cgttcagttc cgcttttaca gatttctcgt
     4381 gctcgctgaa tgcggctttc agcatctcgc cggtattctg ctgctgcgct ttcgatttct
     4441 gctctaagtc cttcgccagc gttaaaagac tgttcataaa tggctccctt cagtcggatg
     4501 tttcgccccc cgtccgggtc ggcaatgctg atactgcttt ttgtggtgcg gaccacctca
     4561 aaacctgcgc tttccagcgc ctcagtgaca tcctgacggg ttttcagctc cccggatgag
     4621 gcaagagcaa gcagaccgca tgtaatcgct tctgcagcct cctgc
//
