LOCUS       NZ_CP044441             5277 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli strain HeN100 plasmid pHeN100_04, complete
            sequence.
ACCESSION   NZ_CP044441
VERSION     NZ_CP044441.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12868540
            Assembly: GCF_026427755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Peng,Z., Hu,Z., Li,Z., Li,X., Jia,C., Zhang,X., Wu,B., Chen,H. and
            Wang,A.X.
  TITLE     Characteristics of a Colistin-Resistant Escherichia coli ST695
            Harboring the Chromosomally-Encoded mcr-1 Gene
  JOURNAL   Microorganisms 7 (11), 558 (2019)
   PUBMED   31726755
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Peng,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2019) College of Veterinary Medicine, Huazhong
            Agricultural University, NO.1 Shizishan Street, Wuhan, Hubei 430070,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026427755.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 05:55:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,874
            CDSs (total)                      :: 4,752
            Genes (coding)                    :: 4,500
            CDSs (with protein)               :: 4,500
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 63 of 252
            Pseudo Genes (incomplete)         :: 195 of 252
            Pseudo Genes (internal stop)      :: 45 of 252
            Pseudo Genes (multiple problems)  :: 47 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044441.1.
            Bacteria and source DNA available from Xiangru Wang, College of
            Veterinary Medicine Huazhong Agricultural University, China.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HeN100"
                     /isolation_source="ENVO:00010483"
                     /db_xref="taxon:562"
                     /plasmid="pHeN100_04"
                     /geo_loc_name="China: Henan"
                     /lat_lon="38.98 N 77.11 E"
                     /collection_date="2018-10"
     gene            complement(<73..654)
                     /locus_tag="HeN100_RS24355"
                     /old_locus_tag="HeN100_01850"
                     /pseudo=""
     CDS             complement(<73..654)
                     /locus_tag="HeN100_RS24355"
                     /old_locus_tag="HeN100_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(644..967)
                     /locus_tag="HeN100_RS01705"
                     /old_locus_tag="HeN100_01855"
     CDS             complement(644..967)
                     /locus_tag="HeN100_RS01705"
                     /old_locus_tag="HeN100_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1033..1224
                     /locus_tag="HeN100_RS01710"
                     /old_locus_tag="HeN100_01860"
     CDS             1033..1224
                     /locus_tag="HeN100_RS01710"
                     /old_locus_tag="HeN100_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2742..3278
                     /locus_tag="HeN100_RS01715"
                     /old_locus_tag="HeN100_01880"
     CDS             2742..3278
                     /locus_tag="HeN100_RS01715"
                     /old_locus_tag="HeN100_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024218429.1"
                     /translation="MKDDKAVVVFTGKDLNIMRAEGGSGYWLAKAERLNDAKYLIAVRN
                     RRETWAVKDLEHGTGFLIAKITGCFKNPDYDDRNVITFEEYAEISVKKAWKMLTGGQRY
                     PVAYLTAEEAFSRIGITLEQLEWKKLQTSTLSQPIPSAEAPKKLSLNEAVERAKQDISN
                     ATDIDVSSITITINI"
                     /name="hypothetical protein"
     gene            3334..4158
                     /locus_tag="HeN100_RS01720"
                     /old_locus_tag="HeN100_01885"
     CDS             3334..4158
                     /locus_tag="HeN100_RS01720"
                     /old_locus_tag="HeN100_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000213997.1"
                     /translation="MTTTIYREFEFAACDSKWSVDGHLRRDVIEKKAIHIDFKGKEAIS
                     LMCGDHPMIVLMQAMQLKVITPEQYASLMSLYMDVEDFTFGMVVLDKHSGAILVGMYTP
                     PMIAIKSKQIVYDLGSTGGEFAAHCLYYAEKRLRKKKRHRGYSTSSVGCKITSAVKYAY
                     RRDRYSGGKVYKIIWKNNGIMYNNIPLSNNSVKNHYRRILKETLKIIHSRYEEKINMRQ
                     NAQLNTAAASLSQTGQPKTQSQPHNQAPSASADKSHQMTMSRAINYARLFSD"
                     /name="hypothetical protein"
     gene            complement(4485..4718)
                     /locus_tag="HeN100_RS01725"
                     /old_locus_tag="HeN100_01895"
     CDS             complement(4485..4718)
                     /locus_tag="HeN100_RS01725"
                     /old_locus_tag="HeN100_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4725..5243)
                     /locus_tag="HeN100_RS01730"
                     /old_locus_tag="HeN100_01900"
     CDS             complement(4725..5243)
                     /locus_tag="HeN100_RS01730"
                     /old_locus_tag="HeN100_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_115722884.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGSSGMRLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQDGRRKFLVLPYGKSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1310..1395)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          1410..1944
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.331"
                     /coverage="99.0"
                     /evalue="3.84e-164"
     Typing          complement(2..654)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.165"
                     /coverage="100.0"
                     /evalue="7.129999999999999e-138"
ORIGIN
        1 gttcgggtct gcaatgctga tactgcttcg cgtcaccctg accacttcaa gccccgcctc
       61 tgtgagcgtc tgaatcacat cctgacggcc ttttatctcc ccggcatggt aaagcacctc
      121 taggcctcgt gtaacgctct cagcaagcgc ctgtttcgtt tccggcaggt tatcagggag
      181 ggtcagcgtt ctgcggttct ccggggcgtt ggggtcatgc agcccgtaat ggtgattcac
      241 cagtgtctgc caggcatcaa ttctcggcct gtctgcccgg tcgtagtacg gctggaggcg
      301 ttttccgctc tgtagctcca tgttcggaat gacaaaatta agctcaagcc gccccttgtc
      361 ctggtgctcc acccacagga tgctgtactg atttttttcg agaccgggca tcagtacgcg
      421 ctcaaagctc tccatcactt tttcacgtcc tcccggcggc agttccttct ctgcgaacga
      481 cagaacaccg gacgtgtatt tcttcgcaaa tggcgtggca tcaataagct cccggacttc
      541 ttccgggtta ccctgaagta ccgttgcacc ttcccggtta cgctccctcc ccagaaggta
      601 atcaaccgga ccactgccac caccttttcc ccgggcatga aacttaacta tcatcccgcg
      661 ctccctgttc cctgacgacc tgccgtaagc tgcgcaactc tctctcgatg gccatcagtg
      721 cggccaccac ctgaacccgg tcgccggaag accactgccc gctgttcacc ttcctcgccg
      781 tctgattcag gttattcccg atggcagcca gctgacgcag taacggcggt gccagtgtcg
      841 gcagcttccc ggaacgggca accggctcac ccaggcagac ccgccgcatc cataccgcca
      901 gttgtttacc ctcacagcgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
      961 tgagcatcct ctcgcgtttc atcggtatca ttacccccat gaacagaaat ccccccttac
     1021 acggaggcat cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     1081 agccagacat taacgctgct ggagaaactc aacgaactgg acgcggatga acaggcagac
     1141 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     1201 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgcctg
     1261 tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcaggttt tagcgggtgt
     1321 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaatcatg
     1381 cggcatcagt gcagattgta agaaaagagc gccatatgcg gtgtgaaatg ccgcacagat
     1441 gcgtaaggag aaaatgcacg ttccggcgct cttccgcttc ctcgctcact gactcgctac
     1501 gctcggtcgt tcggctgcgg cgagcggagt cggctcactc aaaggcggta atacggttat
     1561 ccacagaatc aggggataac gccaaaagaa acatgtgagc aaaaaacgag aacccggaaa
     1621 aggccacgcc gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaatcg
     1681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1741 tggaagctcc ctcgtgcgct ctcctgttcc tacctttcgg tttactggtg tcactccgct
     1801 gttatggccg cgtttgtctc attccacgcc tgacactcgg ttccgggaag gcagttcgct
     1861 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgtc ttatccggta
     1921 actatcgact tgagtccaac ccggaaagac acgacaaaac gccactggca gcggccattg
     1981 gtaaacagga tgtgcgagag atgtatcgag agttcttgaa gtggtgccct tagtgcggct
     2041 acactggaag gacagatttg gtggcatgtc tcgcacaaga ctgttaccac ggttagagaa
     2101 tctgccagga ttaactaacc ttcgaaaaac cacctgccag ggtggttttt tcgttttcag
     2161 agctagagat tacgcgtaga aaaaaaggat ctcaagaaga tcctttttgt agacagctta
     2221 tagatgattc ccgtgaactg aacgcgtata tccaatagct atattaaaca tttcagcttg
     2281 acctggattc agatagcacg ttctcagatc tactctcaca agcaacccat taccttcccg
     2341 ctcagcatag ataccacgag acctgcaaag atctattgat tgctctacca gcttttcagt
     2401 aatgcgctgt ctaccgggag agcgcaggtc aagatagaaa ttactaacca tcgtaccagc
     2461 ttcgctagaa agacgattga tggtcgtttc aatacatgaa tctatttgct gcatagatat
     2521 cgacatataa cccccaaaaa gaataacatc aaaataagat acacataaaa aaatataaaa
     2581 tcaaataata acaacttaag aacaaaataa gcaaaaataa gaaagagata agcacagaat
     2641 ttgtagactt gcttttatcg cataaataaa accccacccc ctcatttaat cgtatataat
     2701 caaaatcatg acgataaaaa actaaagcat gggatttcta tatgaaagat gataaagcag
     2761 ttgtcgtatt tacaggcaaa gatctaaaca taatgagagc tgagggaggt tcaggttatt
     2821 ggctagcaaa agcagaacgg cttaatgacg ccaaatatct catcgcagta agaaaccgga
     2881 gagaaacatg ggcggtgaaa gacctcgaac acggaactgg ctttctgatt gcaaagatca
     2941 ccggatgctt taaaaaccct gattacgacg acagaaacgt aattacattt gaggaatatg
     3001 ctgaaatttc agtgaaaaag gcatggaaaa tgctaactgg cggtcagcgt tatccggttg
     3061 cttatctgac agcagaggaa gctttttccc gtatagggat aacgctagag cagctggagt
     3121 ggaaaaaact ccagacttca acactatctc agccaatccc ctctgcagaa gcgccgaaaa
     3181 aactaagcct caacgaggca gttgaaagag ctaagcaaga tataagcaat gcaactgata
     3241 tcgatgtatc ttctattacg ataactatca atatctgatt ttttcgtata tctggtatct
     3301 taacgatagt cgaaaaagaa ttaagccatt gatatgacaa caacaatata cagagagttt
     3361 gaatttgctg cttgtgatag caaatggtca gttgatggac atcttcgaag agatgttatt
     3421 gaaaaaaaag caatacatat agatttcaaa ggaaaagaag caatatccct tatgtgtggt
     3481 gatcatccga tgatcgtcct tatgcaagct atgcagctga aagtcataac tccagagcag
     3541 tacgctagct taatgagtct ctacatggat gtagaagact tcaccttcgg catggtggtc
     3601 ctcgataaac actcaggtgc tatattagta ggtatgtata cccccccgat gattgcgata
     3661 aaaagcaaac aaatagttta cgatttaggt tcaactggcg gcgaatttgc agcacattgt
     3721 ctctactatg cggaaaaaag gcttagaaaa aaaaagcgtc atcgtggata ctctacatcg
     3781 tcagtgggat gtaagatcac cagcgctgtc aaatatgcat acagacgaga tcgctactcc
     3841 ggtggtaaag tatacaaaat catttggaaa aataacggca tcatgtacaa caacataccg
     3901 ttatctaaca actcggtaaa aaaccattac cggcggattt taaaagaaac attaaagatc
     3961 attcatagca ggtacgaaga gaaaataaac atgagacaga atgcccagtt aaatacagcg
     4021 gcagcctcgc tctctcagac gggtcagccg aaaacacaaa gtcagccaca taaccaagca
     4081 cctagtgcga gtgctgataa aagccatcag atgactatgt ctcgagccat taactatgct
     4141 cgcctttttt ctgactgata ctgtacagct agatttacca taatccctta ttgtacgcac
     4201 cgctaaaacg cgttcatcgc gatcacggca gcagacaggt aaaaatggca acaaaccacc
     4261 cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgc atgaatacct atgcaaccag
     4321 atgatacagg ccacattaac ccctcttaat ccactgaagc tgccattttt catggtttca
     4381 ccatcccagc gaaggaccat ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc
     4441 cagggataac gcatgttcgc gctcctgtat cagccgttcc tctcttatct ccagttctcg
     4501 ctgtataact ggctcaagcg ttctgtcctc tcgctcaagt gctgcacctg ctgactcaac
     4561 tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa catcttctgc
     4621 aattccacga aggcgctctc ccattcgttc agccgctgca tataatcctg ttgcagctgc
     4681 tctaaggcgt tcagcaaatg tgtttccagc tctgtcattc cgtatcactc cgtcagttgt
     4741 atccagtcct tcccctgata gtgagtcacc tctgctgatt tcccgtacgg aaggaccaga
     4801 aatttccttc tcccgtcctg cacaaactcc acgccccatg tcttcgcgtt cagtttccgc
     4861 aatgtctctt cctgtaccct gatttcttcc aggttcgtct gtatcctccc gccgagatac
     4921 cagagcgtcc cgccactcgc ggtaaacagg agaaagacta tccccagcaa cctcatgccc
     4981 gaactccctg ccagctttaa catgtccttc cggtgctgca tcatcgcctc tttcacccct
     5041 tcccggtgtt ttttcagcga ttcctccgtc gaggccgtaa actgggctat agcgcctttg
     5101 attttcgtct cgtttgaggt cacagcctcg cttacagatt cgccgagcct gctgaattcg
     5161 tcgttcagca ttttctctgt agattcggct ctctctttca gctttttctc gaactccgcg
     5221 cccgtccgta aaagattgct cataaaacgc tcctttcagt ctgatattcc tcccgcc
//
