LOCUS       NZ_CP044327             2056 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Enterococcus faecium strain 15-307-1 plasmid p15-307-1_11, complete
            sequence.
ACCESSION   NZ_CP044327
VERSION     NZ_CP044327.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08093650
            Assembly: GCF_002973755.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Guerin,F., Sassi,M., Dejoies,L., Zouari,A., Schutz,S., Potrel,S.,
            Auzou,M., Collet,A., Auger,G. and Cattoir,V.
  TITLE     Cfr(D), a new plasmid-mediated linezolid resistance determinant in
            Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Guerin,F., Sassi,M., Dejoies,L., Zouari,A., Schutz,S., Potrel,S.,
            Auzou,M., Collet,A., Auger,G. and Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
REFERENCE   3  (bases 1 to 2056)
  AUTHORS   Sassi,M., Guerin,F., Zouari,A., Auzou,M., Fines-Guyon,M., Potrel,S.,
            Collet,A., Auger,G., Bonnet,R. and Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2017) Microbiology, CHU de Rennes, 2 rue Henri Le
            Guilloux, Rennes 35033, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-SEP-2019
            Assembly Method :: Unicycler v. v0.4.8; canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 450.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:06:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,060
            CDSs (with protein)               :: 3,060
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 86 of 146
            Pseudo Genes (internal stop)      :: 31 of 146
            Pseudo Genes (multiple problems)  :: 20 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044327.1.
            Bacteria and source DNA available from NRC for Enterococci.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="15-307-1"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p15-307-1_11"
                     /geo_loc_name="France: Corbeille-Essonnes"
                     /lat_lon="48.6103 N 2.4748 E"
                     /collection_date="2015"
                     /collected_by="NRC for Enterococci"
     gene            303..458
                     /locus_tag="CWC51_RS16405"
                     /old_locus_tag="CWC51_016860"
     CDS             303..458
                     /locus_tag="CWC51_RS16405"
                     /old_locus_tag="CWC51_016860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            525..1127
                     /locus_tag="CWC51_RS16410"
                     /old_locus_tag="CWC51_016865"
     CDS             525..1127
                     /locus_tag="CWC51_RS16410"
                     /old_locus_tag="CWC51_016865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1263..1520)
                     /locus_tag="CWC51_RS16415"
                     /old_locus_tag="CWC51_016870"
     CDS             complement(1263..1520)
                     /locus_tag="CWC51_RS16415"
                     /old_locus_tag="CWC51_016870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1521..1742)
                     /locus_tag="CWC51_RS16420"
                     /old_locus_tag="CWC51_016875"
     CDS             complement(1521..1742)
                     /locus_tag="CWC51_RS16420"
                     /old_locus_tag="CWC51_016875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          complement(1186..1361)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          526..1055
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tatatttcgg tagaaggagg tcggggttcg gggcggagcc ctgaggtttg ttttttggtt
       61 ttggtgttta tgaattgggc ggaggagggg gggactacga cacccccctt attggtcatt
      121 ggtcattggt cgatagctgt ttagccttac tctcccaagg gtttcagcgt ttttttttga
      181 caaaatcagt aatgccaagg gtttaaggcc tattattcgc taattagtat ctaaactgtt
      241 gcaccttagt tacttttttg ttactatggt ttttaagaaa aagtcgttgg aggatacgaa
      301 ttatgatcag taatgaaaaa aagagagtcg ttgtaacgct tactaaggaa gtggcagata
      361 atcttgataa aattgcgaaa caaatgggtc ttagtaagtc tggtcttatt acaatttggg
      421 ttaatgctaa cggaaaggaa aatgagcaaa aaaaatagca aaagcattag tgttggcgca
      481 ctgcttttgc tgttagactg ttgttcaatt aaggtaatta tagcatgacg agtagaaaaa
      541 ctaaagatga acgcacaagg aattggacgt ttgtagtcta tcctgaatcg gctcctgaaa
      601 attggaggga gtttttagat gagttacatg ttccttgggt tgagagtcca cttcatgata
      661 aagatgtgaa tcctgacggt gaattgaaaa agcctcattg gcatgttttg ataatgttta
      721 gcggtaaaaa gacgtatgat caaatacgtg agataactca aaagcttcgg tctccaaacc
      781 cgcaaaaatg tgctaatgca aaaggtatgg tgcgatattt tgctcatatg gataatccag
      841 aaaaatttca atatgctaaa agtgatatta tcgctcatgg tggtgcagaa attgctagtt
      901 atttgtcagt aacgagtgca gaacgatatg agttgattag agagatgatg tcgtttgttg
      961 attctaagaa tatcactgaa atcaaagatt taattgacta tgcaatgtca gaaagatttg
     1021 atgattggtt tccgcttctt tgtgataatt cagcttatat tatcgggcaa tatataaaat
     1081 cgaatcgtca tggtggaagt gtaaattcaa aaataaataa aggataagag ctgttaattt
     1141 cagctctttt tttgtgcgtg aaagtcttag tcactatgac caaaactacg gacgaaacta
     1201 tgaccaaaag taggtactat attatgaccg aaaataggtc caaaatggat ttttttgtga
     1261 tatcaaaaaa tataaggttg ttttgtaaga aaaccagttg gcacagccga cttagccacc
     1321 ttcctgtagg aaggtgctat cagttttccc ttatgctctt tttgttcttc agagtttaat
     1381 tccagaatat ttgtgagaag taggtcaaag aggtcggtgg tttcttcgac ttttccgccg
     1441 gtcaattcga taaaatcatt ggaatatgat tctagcaatt tttctagttg ttcaacgttg
     1501 atagttgttt ctttaggcat ttactcacct tctttggtgg ttatgacttt ttctagtttt
     1561 ttaagggctt ctgggctgaa aagatagttt cttctaccgg cagagcgata ttcttttcct
     1621 tcagtcagat gttctttcgc taagcgcaaa acgtaagctt ggttgtaatc aagttcttgt
     1681 gcaagttcgc tggtcgtcat aatttcggcc aaagtgaatt tttctgattt tatcactctc
     1741 atttatgctc cttccattta acgtccgtct atattacgtg agtacgtcgg acttacgaca
     1801 taagcttata atataagttt tctgtgttgt ggtcaatttt atattatcga aatgactagt
     1861 agaaaaaatc aatgctctaa aaagggtcta ttttgcgttt taagcgattt agcgtctatt
     1921 tcgtttagtt atcggcgcaa tcgttaaaac acgtaagaga agcgagaatg gagcctgtaa
     1981 ggcgactggt agcgaaacgg tcgtgttaga ttgcaaaagc cgatagctga acgaaatagt
     2041 aagcgtggcg cctgca
//
