LOCUS       NZ_CP044322            11825 bp    DNA     circular CON 13-OCT-2023
DEFINITION  Enterococcus faecium strain 15-307-1 plasmid p15-307-1_06, complete
            sequence.
ACCESSION   NZ_CP044322
VERSION     NZ_CP044322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08093650
            Assembly: GCF_002973755.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Guerin,F., Sassi,M., Dejoies,L., Zouari,A., Schutz,S., Potrel,S.,
            Auzou,M., Collet,A., Auger,G. and Cattoir,V.
  TITLE     Cfr(D), a new plasmid-mediated linezolid resistance determinant in
            Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   Guerin,F., Sassi,M., Dejoies,L., Zouari,A., Schutz,S., Potrel,S.,
            Auzou,M., Collet,A., Auger,G. and Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
REFERENCE   3  (bases 1 to 11825)
  AUTHORS   Sassi,M., Guerin,F., Zouari,A., Auzou,M., Fines-Guyon,M., Potrel,S.,
            Collet,A., Auger,G., Bonnet,R. and Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2017) Microbiology, CHU de Rennes, 2 rue Henri Le
            Guilloux, Rennes 35033, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-SEP-2019
            Assembly Method :: Unicycler v. v0.4.8; canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 450.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/13/2023 02:06:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,206
            Genes (coding)                    :: 3,060
            CDSs (with protein)               :: 3,060
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 86 of 146
            Pseudo Genes (internal stop)      :: 31 of 146
            Pseudo Genes (multiple problems)  :: 20 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044322.1.
            Bacteria and source DNA available from NRC for Enterococci.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="15-307-1"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p15-307-1_06"
                     /geo_loc_name="France: Corbeille-Essonnes"
                     /lat_lon="48.6103 N 2.4748 E"
                     /collection_date="2015"
                     /collected_by="NRC for Enterococci"
     gene            1..933
                     /locus_tag="CWC51_RS16200"
                     /old_locus_tag="CWC51_016640"
     CDS             1..933
                     /locus_tag="CWC51_RS16200"
                     /old_locus_tag="CWC51_016640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="CWC51_RS16205"
                     /old_locus_tag="CWC51_016645"
     CDS             950..1420
                     /locus_tag="CWC51_RS16205"
                     /old_locus_tag="CWC51_016645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="CWC51_RS16210"
                     /old_locus_tag="CWC51_016650"
     CDS             1504..1956
                     /locus_tag="CWC51_RS16210"
                     /old_locus_tag="CWC51_016650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="CWC51_RS16215"
                     /old_locus_tag="CWC51_016655"
     CDS             2083..3264
                     /locus_tag="CWC51_RS16215"
                     /old_locus_tag="CWC51_016655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..>3530
                     /locus_tag="CWC51_RS16220"
                     /old_locus_tag="CWC51_016660"
                     /pseudo=""
     CDS             3354..>3530
                     /locus_tag="CWC51_RS16220"
                     /old_locus_tag="CWC51_016660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /name="bacteriocin"
     gene            complement(3680..4828)
                     /gene="tnpB"
                     /locus_tag="CWC51_RS16225"
                     /old_locus_tag="CWC51_016665"
     CDS             complement(3680..4828)
                     /gene="tnpB"
                     /locus_tag="CWC51_RS16225"
                     /old_locus_tag="CWC51_016665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4845..5249)
                     /gene="tnpA"
                     /locus_tag="CWC51_RS16230"
                     /old_locus_tag="CWC51_016670"
     CDS             complement(4845..5249)
                     /gene="tnpA"
                     /locus_tag="CWC51_RS16230"
                     /old_locus_tag="CWC51_016670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            <5398..5514
                     /locus_tag="CWC51_RS16795"
                     /old_locus_tag="CWC51_016675"
                     /pseudo=""
     CDS             <5398..5514
                     /locus_tag="CWC51_RS16795"
                     /old_locus_tag="CWC51_016675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /name="bacteriocin"
     gene            6320..6493
                     /locus_tag="CWC51_RS16235"
     CDS             6320..6493
                     /locus_tag="CWC51_RS16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6897..8051
                     /locus_tag="CWC51_RS16240"
                     /old_locus_tag="CWC51_016680"
     CDS             6897..8051
                     /locus_tag="CWC51_RS16240"
                     /old_locus_tag="CWC51_016680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_060807324.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            8024..8467
                     /locus_tag="CWC51_RS16245"
                     /old_locus_tag="CWC51_016685"
     CDS             8024..8467
                     /locus_tag="CWC51_RS16245"
                     /old_locus_tag="CWC51_016685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8769..9149
                     /locus_tag="CWC51_RS16250"
                     /old_locus_tag="CWC51_016690"
     CDS             8769..9149
                     /locus_tag="CWC51_RS16250"
                     /old_locus_tag="CWC51_016690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9131..10045
                     /locus_tag="CWC51_RS16255"
                     /old_locus_tag="CWC51_016695"
     CDS             9131..10045
                     /locus_tag="CWC51_RS16255"
                     /old_locus_tag="CWC51_016695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10005..10283
                     /locus_tag="CWC51_RS16800"
                     /old_locus_tag="CWC51_016700"
     CDS             10005..10283
                     /locus_tag="CWC51_RS16800"
                     /old_locus_tag="CWC51_016700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10285..10974
                     /locus_tag="CWC51_RS16260"
                     /old_locus_tag="CWC51_016705"
     CDS             10285..10974
                     /locus_tag="CWC51_RS16260"
                     /old_locus_tag="CWC51_016705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9132..10042
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagg tgaactactc accacttagc
     3541 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
     3601 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
     3661 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
     3721 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
     3781 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
     3841 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
     3901 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
     3961 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
     4021 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
     4081 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
     4141 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
     4201 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
     4261 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
     4321 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
     4381 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
     4441 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
     4501 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     4561 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     4621 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     4681 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     4741 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     4801 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     4861 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     4921 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     4981 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     5041 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     5101 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     5161 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     5221 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     5281 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     5341 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     5401 cctaattctg ttaaaatgtt agtaaatatt agaggtaaac aaactatgcg tacttgttac
     5461 cttatgagtt ggaatgctag tagtagaaca gccaaatatt attactacat ttagtaaaaa
     5521 caaaacattc tttctataca ataaaatagc gtatagagag gatgttttta atttgaaaag
     5581 gagtggttat ctttatgaaa aatagatcat ttgctatatt agtattatcc gtaattttat
     5641 tagatatatt gttaataaca ttagctttaa aaactttaaa tttagtaaca agttttgtca
     5701 ttataccagt aattatagga atgtctattt atttcatcaa aaaaaataaa caattttttt
     5761 gaatgaaatt acgggcgaaa ataatcactc aaaattatta ataaaaacga atcaactact
     5821 ttttctgaag aaacatacaa ccgtaaacaa caattgttgt ttacggttgt aaattatcaa
     5881 actatatggt gacttgttgc ttaaaatagg ttgaaatttc gtttattttt tttgagaaat
     5941 tttacccacg tactacccat gtacctatcc acgtattacc catgtacccc cccatgacaa
     6001 atgggcggag gggcttgcgg cgcttggtat taaaggcatt atccgcctcc caggtttctg
     6061 accattttct ctcataagtg gggtttttac cgcgttgaga gaaaatggtc agaaacctaa
     6121 acgatcggca aagaaaacat accaagcgcc acaagcaaaa aaagttagag taacttccag
     6181 aaaagaaaaa tttttagaac aacttttgaa aatttgattt tgatttatat ccgttcaatt
     6241 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     6301 aaaagtggag gtataaccta tgttaaaaaa aactgtgttg gtactgggtt ctatttctct
     6361 aggaatatta ggttttagcc ccatgctatt atccgtaatt ggaaaaaaca attccaacag
     6421 agaacttaaa ttaaattcaa attattacaa cagtcctaac gaagaattgt ttgtaaaaca
     6481 aacggataaa taataaaaaa gactgctctt tcgagcagcc agttaaaaaa taatgagtgt
     6541 ttattcttga aaaagcacgc gcttatgaag cgctaccaaa aatttaccat attttacaaa
     6601 aaataacaag tcttaaaaaa atgacagata aaaatgcaca aagaattaga tatagataac
     6661 cgcgaaaaat caagctttag aaagttgctt ttagctagac caattaaaaa tcacaaagtg
     6721 agcgtctcta tccattggta gaggggctac ttggcatagt gtacattatg ttaactaacg
     6781 ctataaataa cgattatgtt cccaaaacaa tattttatga actataacaa aaatttgtta
     6841 tcgcatattg tatattttaa ttataggtgt ataccactta aatttaacac aaaggcgtga
     6901 ttattttgaa acccaaaaag aaaatttatc taggtctcct cttttccttg atgattggaa
     6961 tttttagtat aggaattaca aatacctatg cttcagaaaa ttctaatttc aatatcaact
     7021 cccaaccaat tgaagaacaa gcaattaatt ttgcgaaaac tgattttgaa cactccataa
     7081 cttcaattct tttaatggaa aataacaccg aaactgatat taactatact cttggttcgc
     7141 catttaaaat gctcgatgga gaaaatacgg caaatagttc tattttcttt ccagtatttt
     7201 cagacgctga taatacgatt aaatatattt ataccgtcaa aaaaaataca aatggagaat
     7261 attcaggaag tatttcacaa tttttagcaa aagaacttca aaatctagta gatagtaata
     7321 taacagatac aaccttattt tttcaagata atagcattta ttattccgat aatggacaaa
     7381 ttaaactact ttggaaatca cctttaagta cagaaaaaaa tggtgtttct tcctttacag
     7441 aaaaaaatga tcttatatcc gtaaatataa aaaataaaat taaaaatttc tctaaaaaac
     7501 aaaattttcc tgcgattaaa cgtgtaaaaa gtgtctcctc cggagataac tttattatga
     7561 ttaattggcg tatagcagaa acccaaacaa gttttccgtg gtgtgctgct tacgtagaag
     7621 ctgctatctt atgtaacaaa acagatgtac atgctacttc agctagagcc attatgcatt
     7681 atacctatcc taatttaaac aacagtcaaa ttgaatcaaa aagtcttaat caagatcaaa
     7741 taataaaata tgcaaattac tgtggtgttt atcctaaaaa agtagcacga atactaaacc
     7801 tttcagaggt ccaaacacaa ctaagaaaag gaaatgccat ttatatggga actgctggtt
     7861 atggaaatta tagtaacagc cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg
     7921 gttatcaaac ttactatgtg tggaatcctt ggtataacta cccaactgta ataagtgcaa
     7981 atgcaggacc agttacaatt cctgtacctg gaggtggttt tacttgggac aataccatta
     8041 caaattggta aataaaccca ttgctttagt tatgggtcta gtgttagttc ttttcttaac
     8101 tagttgttca aaaaaacaag aaatacaaaa gactgatctg attccttttc taggaacttg
     8161 gcaaactaaa gatcattcag aacgtctagt aatcactaat ttaaatactt caaaaaatat
     8221 agaacaatat accatcttag taaaagagaa caatcataaa attcaaatca ttgataaaaa
     8281 tttaaatata aatgaacaag ttttcattta caaagcaaca aataatttac taacactgcg
     8341 ctctaataaa aaacaattag attacacttt tagtttggat tctccaaaaa agctcgtatt
     8401 tattttgaca acgaatgatg ggacagaagg gacagctaaa ccaattgaat tttttaaagc
     8461 tgattgatga atagttgttt gatattacaa aacaataatt tcaccgaagg tgaaacaagc
     8521 gaaactttac aaaaagtggc tagcatttgc tagccttttt tgtattgtgg agcgtgggtt
     8581 ttgaggggga ttcaaggggg agcttgcgcc cctttgaaaa gcggttattt gcaccaaata
     8641 cgtaggtatt tggctagcaa atatagagct tgccaaaccc ttttagtgag ctttactcat
     8701 tgtacctttt taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg
     8761 tgggacttat gagcgaacaa aatcaaaatt tggctagcga tcattctaaa aaatatacct
     8821 atcgatccga gccaaaacaa atcagttttc gagtgagcga atctgaattt gcaaagctga
     8881 agcagtcagc tgaagctttg caaatgagtg tgccggcttt cgtaaaagcc aaggcacaaa
     8941 acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt
     9001 tagccaaagc tggagggaat atcaatcaaa ttgctaagtg gtgtaataca caccaacacg
     9061 acgttgctcc tggagacgcc caacgcttat ccgaaaacct aaaaatcatg caaaaggagc
     9121 tacaaaagat atggcaacag ttaaagtaag ccgaaccact tcttgtagtc gtgcaattaa
     9181 ttacgctgaa ccacgagcaa ccattaaaac aggaataaac tgtgacgtcg attatgccaa
     9241 aagtgaaatg aaacaaattc gcatgctgta tggaaaagac gaccgtgtac aggcacactt
     9301 attgattcaa tcttttcgac caggagaaat taccgctgaa aaagctaatc aactaggggc
     9361 agagtatgca aaaaaaatag cccccgatca ccaaatagct atttacacac atacagacaa
     9421 agaccatatt cataatcaca ttgtcattaa ctccgttaat ctggagactg gaaaaaaatt
     9481 tcaagctcat ggtcaagcat ttctaaacaa atgttatgac ctaaatgacg agatttgtct
     9541 tgcacataac ttaccaatca ctgagcgagg aaagaaacca gaaaaacgaa ccatgtctga
     9601 aataaaatta aaagaaaaaa atgaacctgt ttggaaagac gaaattcgga ttgcgatcga
     9661 tcaaacaatg aaaaacaaaa aaaccagaac ctatgaccaa ttttgtaatt ccctcaaaac
     9721 attggggatt cattgcttca atcgcgggaa aaatttcact tacgaattga tccacaaaaa
     9781 gaaaaaagta cgatccaaca gactaggaaa ggactacgaa aaggagacga ttttacatga
     9841 gctggacaga cgagaacgaa cctataacca acagcgaacc aacgagctac gaagagctcg
     9901 agctagcatt cgcgaaactg cagaagaagt acgaagagaa caacgaactc ttacgcctga
     9961 acaaccggaa attagaccgg ctcgaagaaa ccatgaacaa cacgttgaaa gaggaacaga
    10021 ccaccaacaa gaattatcgc tctaaaacgt taaaagacat agaaaacgaa ataaagaaac
    10081 aacgtgcttt tctcgttcct tatacgcaag aaaggataca aactacgatt gatttacaag
    10141 ttaaaaaact ccatcaagag ctattagacc agcgaaataa ggaacagaaa aaggtgctta
    10201 ttcaacgatc attggatttt cttgccccct tatgttgctt agtcctgaca ctgttcgcat
    10261 tttggttagt atggggaggc taaaatggca ttttatttac cctatctact catttttgtt
    10321 agtatttcag gaagtatttg gctcatctat aaaatttttc aaacaagata ttcattaaaa
    10381 ggctctaaaa ttcgatttaa gcgatttttc tttcttggct gtatattttc cttgataatc
    10441 gtttcaagcg gcttattcgg cgttttagag ggaaataagc gcgtttctag aagtatttta
    10501 ttaggaaatg tcactcaaaa gtatgaatca gcgcgtgata aaaagaaaaa agagcaagta
    10561 ttagcacaaa aaatagaaaa atttaccgct tgttatgagg acatgaatga tatttttgtg
    10621 aagcaagaaa agcgtttgac ggataaaaat atggaaacac tcactcgact ttatcggaat
    10681 ttgccagaag aacagcaaaa agagtaccaa gaaaaatatg aacaagtaaa aaaagacgtg
    10741 caatatgtga aagatacgaa agtggaagaa gcttgtagtg atttatttgg agatacaatt
    10801 cctttttcaa ctagcgaaca agaacgaaaa gaacgacaac aatcagtcac atatgaacgc
    10861 tatgaaatct tgcttcaaca agcaattaac atacaaaatc tgactaaaaa agaaacagct
    10921 ttaaattatt taaaaagcgt caaggaatgg ttagatcagc aacagcaaaa ttgaatttga
    10981 ttgaaattca tgttaaacta aacatgcaaa tgttcaaaca ttagatacac gaaagcccca
    11041 gagaggtcgg attctctggg gcttttttta ggttgatgtc gcctttactg attttacttc
    11101 ttgtttcggt tattcaacca ataactgaat acagagatac aaactcctac aagaaggggg
    11161 caaataaaca gttcaaacat cagatacacc tccttccagt cgcaagactg tcggataggc
    11221 gacaagttat tataccctac aaaattaaat ctgtgtggca attttaagta tctcttattt
    11281 ccgggttgtt agggcttgcc ctgaccgcct gtagggcgct ttaaatgcca gataaaggtt
    11341 tttatactaa gactgtctta aaacggctta gacaagctct tagagcctca accctccttt
    11401 ttgaacaatg gttttagcaa aggagctgga ggcgactgcg caggtataat gtgagagcgt
    11461 agcgatcaca cctttttggc gaccgtaggg agcgaggttt tgaattagca gtttagaaaa
    11521 gttcgcgaac ctgtttaaag aaaaaataat tgttttttta ggtgggaaat tttgtctccc
    11581 tcttttataa tcttttttta aacctctttt taaacctctt taccgtcggc tgtgtcctta
    11641 ctcccccaag ggatacaaaa ttttataggc atgaaattat ctgtttatag gcatgaaatt
    11701 atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt tttaggtatt
    11761 gtttttagac ataatatgtc ctaaaatgat gtcaataaaa tcatttagac tggagtaaca
    11821 actaa
//
