LOCUS       NZ_CP044282             2056 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Enterococcus faecium strain V2937 plasmid pHVH-V2937-8, complete
            sequence.
ACCESSION   NZ_CP044282
VERSION     NZ_CP044282.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12818041
            Assembly: GCF_008728475.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     The association between vancomycin-resistant Enterococcus faecium
            colonization and infection: A retrospective WGS study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2019) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard alle 30,
            Hvidovre, Copenhagen 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2019
            Assembly Method                   :: Unicycler v. Version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 05:32:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 44 of 110
            Pseudo Genes (incomplete)         :: 65 of 110
            Pseudo Genes (internal stop)      :: 22 of 110
            Pseudo Genes (multiple problems)  :: 16 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044282.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Copenhagen University Hospital Hvidovre, Copenhagen,
            Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="V2937"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pHVH-V2937-8"
                     /geo_loc_name="Denmark:Copenhagen"
                     /lat_lon="55.67 N 12.56 E"
                     /collection_date="2018"
                     /collected_by="Copenhagen University Hospital Hvidovre"
     gene            498..719
                     /locus_tag="F6440_RS16070"
                     /old_locus_tag="F6440_16070"
     CDS             498..719
                     /locus_tag="F6440_RS16070"
                     /old_locus_tag="F6440_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            720..977
                     /locus_tag="F6440_RS16075"
                     /old_locus_tag="F6440_16075"
     CDS             720..977
                     /locus_tag="F6440_RS16075"
                     /old_locus_tag="F6440_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1113..1715)
                     /locus_tag="F6440_RS16080"
                     /old_locus_tag="F6440_16080"
     CDS             complement(1113..1715)
                     /locus_tag="F6440_RS16080"
                     /old_locus_tag="F6440_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1782..1937)
                     /locus_tag="F6440_RS16085"
                     /old_locus_tag="F6440_16085"
     CDS             complement(1782..1937)
                     /locus_tag="F6440_RS16085"
                     /old_locus_tag="F6440_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     Typing          880..1055
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(1186..1715)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtcaaaaa aaaacgctga aacccttggg agagtaaggc taaacagcta tcgaccaatg
       61 accaatgacc aataaggggg gtgtcgtagt ccccccctcc tccgcccaat tcataaacac
      121 caaaaccaaa aaacaaacct cagggctccg ccccgaaccc cgacctcctt ctaccgaaat
      181 atatgcaggc gccacgctta ctatttcgtt cagctatcgg cttttgcaat ctaacacgac
      241 cgtttcgcta ccagtcgcct tacaggctcc attctcgctt ctcttacgtg ttttaacgat
      301 tgcgccgata actaaacgaa atagacgcta aatcgcttaa aacgcaaaat agaccctttt
      361 tagagcattg attttttcta ctagtcattt cgataatata aaattgacca caacacagaa
      421 aacttatatt ataagcttat gtcgtaagtc cgacgtactc acgtaatata gacggacgtt
      481 aaatggaagg agcataaatg agagtgataa aatcagaaaa attcactttg gccgaaatta
      541 tgacgaccag cgaacttgca caagaacttg attacaacca agcttacgtt ttgcgcttag
      601 cgaaagaaca tctgactgaa ggaaaagaat atcgctctgc cggtagaaga aactatcttt
      661 tcagcccaga agcccttaaa aaactagaaa aagtcataac caccaaagaa ggtgagtaaa
      721 tgcctaaaga aacaactatc aacgttgaac aactagaaaa attgctagaa tcatattcca
      781 atgattttat cgaattgacc ggcggaaaag tcgaagaaac caccgacctc tttgacctac
      841 ttctcacaaa tattctggaa ttaaactctg aagaacaaaa agagcataag ggaaaactga
      901 tagcaccttc ctacaggaag gtggctaagt cggctgtgcc aactggtttt cttacaaaac
      961 aaccttatat tttttgatat cacaaaaaaa tccattttgg acctattttc ggtcataata
     1021 tagtacctac ttttggtcat agtttcgtcc gtagttttgg tcatagtgac taagactttc
     1081 acgcacaaaa aaagagctga aattaacagc tcttatcctt tatttatttt tgaatttaca
     1141 cttccaccat gacgattcga ttttatatat tgcccgataa tataagctga attatcacaa
     1201 agaagcggaa accaatcatc aaatctttct gacattgcat agtcaattaa atctttgatt
     1261 tcagtgatat tcttagaatc aacaaacgac atcatctctc taatcaactc atatcgttct
     1321 gcactcgtta ctgacaaata actagcaatt tctgcaccac catgagcgat aatatcactt
     1381 ttagcatatt gaaatttttc tggattatcc atatgagcaa aatatcgcac catacctttt
     1441 gcattagcac atttttgcgg gtttggagac cgaagctttt gagttatctc acgtatttga
     1501 tcatacgtct ttttaccgct aaacattatc aaaacatgcc aatgaggctt tttcaattca
     1561 ccgtcaggat tcacatcttt atcatgaagt ggactctcaa cccaaggaac atgtaactca
     1621 tctaaaaact ccctccaatt ttcaggagcc gattcaggat agactacaaa cgtccaattc
     1681 cttgtgcgtt catctttagt ttttctactc gtcatgctat aattacctta attgaacaac
     1741 agtctaacag caaaagcagt gcgccaacac taatgctttt gctatttttt ttgctcattt
     1801 tcctttccgt tagcattaac ccaaattgta ataagaccag acttactaag acccatttgt
     1861 ttcgcaattt tatcaagatt atctgccact tccttagtaa gcgttacaac gactctcttt
     1921 ttttcattac tgatcataat tcgtatcctc caacgacttt ttcttaaaaa ccatagtaac
     1981 aaaaaagtaa ctaaggtgca acagtttaga tactaattag cgaataatag gccttaaacc
     2041 cttggcatta ctgatt
//
