LOCUS       NZ_CP044278             7670 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Enterococcus faecium strain V2937 plasmid pHVH-V2937-4, complete
            sequence.
ACCESSION   NZ_CP044278
VERSION     NZ_CP044278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12818041
            Assembly: GCF_008728475.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     The association between vancomycin-resistant Enterococcus faecium
            colonization and infection: A retrospective WGS study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2019) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard alle 30,
            Hvidovre, Copenhagen 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2019
            Assembly Method                   :: Unicycler v. Version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 05:32:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 44 of 110
            Pseudo Genes (incomplete)         :: 65 of 110
            Pseudo Genes (internal stop)      :: 22 of 110
            Pseudo Genes (multiple problems)  :: 16 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044278.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Copenhagen University Hospital Hvidovre, Copenhagen,
            Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="V2937"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pHVH-V2937-4"
                     /geo_loc_name="Denmark:Copenhagen"
                     /lat_lon="55.67 N 12.56 E"
                     /collection_date="2018"
                     /collected_by="Copenhagen University Hospital Hvidovre"
     gene            join(7262..7670,1..80)
                     /locus_tag="F6440_RS15950"
                     /old_locus_tag="F6440_15950"
     CDS             join(7262..7670,1..80)
                     /locus_tag="F6440_RS15950"
                     /old_locus_tag="F6440_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060763418.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKEA
                     VTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQQK
                     LLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            376..657
                     /locus_tag="F6440_RS15955"
                     /old_locus_tag="F6440_15955"
     CDS             376..657
                     /locus_tag="F6440_RS15955"
                     /old_locus_tag="F6440_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368064.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIENDF
                     DQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            659..1075
                     /locus_tag="F6440_RS15960"
                     /old_locus_tag="F6440_15960"
     CDS             659..1075
                     /locus_tag="F6440_RS15960"
                     /old_locus_tag="F6440_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368067.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1830..2978)
                     /gene="tnpB"
                     /locus_tag="F6440_RS15970"
                     /old_locus_tag="F6440_15970"
     CDS             complement(1830..2978)
                     /gene="tnpB"
                     /locus_tag="F6440_RS15970"
                     /old_locus_tag="F6440_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(2995..3399)
                     /gene="tnpA"
                     /locus_tag="F6440_RS15975"
                     /old_locus_tag="F6440_15975"
     CDS             complement(2995..3399)
                     /gene="tnpA"
                     /locus_tag="F6440_RS15975"
                     /old_locus_tag="F6440_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3634..4734
                     /locus_tag="F6440_RS15980"
                     /old_locus_tag="F6440_15980"
     CDS             3634..4734
                     /locus_tag="F6440_RS15980"
                     /old_locus_tag="F6440_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_222718176.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYVYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSLT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKIARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            4707..5150
                     /locus_tag="F6440_RS15985"
                     /old_locus_tag="F6440_15985"
     CDS             4707..5150
                     /locus_tag="F6440_RS15985"
                     /old_locus_tag="F6440_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917876.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            5451..>5596
                     /locus_tag="F6440_RS16495"
                     /pseudo=""
     CDS             5451..>5596
                     /locus_tag="F6440_RS16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            6284..7252
                     /locus_tag="F6440_RS16000"
                     /old_locus_tag="F6440_16000"
     CDS             6284..7252
                     /locus_tag="F6440_RS16000"
                     /old_locus_tag="F6440_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002368293.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIANFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLAE
                     LERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
                     /name="replication initiation protein"
     Typing          6285..7252
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaagaacc cctttctgtt actgaagaaa ctcaaaaagg acaaaacaag gagaaaaaat
       61 ggtggcaatt ctggcaataa cccaacaatt tttattcata aaaattgttg ggttactttc
      121 tttttttgct tttacaagca tagcgacatg atctcacgtc gctatttttt aaagagcata
      181 aggaaacgcg gtatcacttt cttacgaaag gtgctaggta ggctgtgcct accacgtttc
      241 taagaaaaaa atttgctatc actttttcgt cattgtggat agcttatgta tagtgtttcg
      301 gcaaaaaaca gctatacaaa tactatattt gtatgatata cataaattat acacaaatgc
      361 gaaaggaatt cttctatgga cattttagtt cggaatattg atccaaaagt tatttcagaa
      421 atagatagac gttgccaaga actcacaaca aaaactggca aaaaatggag ccgtaatgcg
      481 tacctaaaag ttctaatcga aaacgatttt gaccaggcac ttaggcatta caaaaaagat
      541 caattcgatt tattactcga aaaatttatc gatatacaag caaaaaatac tgaaacattg
      601 aacgactatg ttcatacaaa caaccaactc attgaaacac ttatcggagg aatataacat
      661 gagaaaaact tctgttcaaa aacacgttac cctttatccc agtcacattg acgttttaga
      721 ttccatcatc gaaaaaaatc cagatatcaa taactactcg gaagccattc gttttctatg
      781 tatgagttat gaacctaata ctaatacgac aactaccagc aaccaagcaa aactaaatgc
      841 gatgggaaaa gaaatttcta ttatgtccga aatcattgca gaatttgctg atttatatct
      901 aaaagatact ggtgttctta tcggtacaaa ttctaaagta tatcaagaag tgaaagaaaa
      961 aattgaaaat caactcaatc cctcccgacg aaatcaaccc aaaaatcgtc aaaaagaaac
     1021 caatcaaaca ttcacttcta gaaattttga cccaacaaac tttctaaaaa gatgaaaaag
     1081 aaaccgaaaa ttttttctat ttctcagttt cttttttagg gggcagtctt ccagcaagat
     1141 ttgcaagtta ccggtaactt gcacaaaaaa agaaaaatcc gcttgtgcga atccttcttt
     1201 ttgcattttg gggcgctgcc ccaatctcca aatatcggtt atttaatagc gtggtaactc
     1261 aaaaaaatag aacgtactct gaaataaaaa aagactgctc taacgagcag ccaataaaaa
     1321 ataatgagcg tttattcttg aaaaagcagc gcgcttatga agcgctgcca aaaatttacc
     1381 atattttatg aaaaataaca agtttctaaa aaggaatcgg ttaagattca caaagaatcg
     1441 taaacagaca atagcggaaa atcaagcttc cgaacgtacc ttttagctag accaatgaaa
     1501 aatcacaaag tgagcgtctc tatccattgg tagaggggct acttggcata gtgtacatta
     1561 tgtctactag ctcgataaat aacgattatg ttcccaaaac aatattttat gaactataac
     1621 aaaaatttgt tatcgcatat tgtatatttt aattataggt gtatatcacg tgaactactc
     1681 accacttagc taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag
     1741 catacttgat agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc
     1801 gtagttccta cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga
     1861 ccttcggtca gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca
     1921 ggacaagtcc attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa
     1981 caaatctgac tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca
     2041 gccttgtatt gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct
     2101 aatttatgat ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga
     2161 tttttgatca tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt
     2221 tcatgcaatc tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg
     2281 gtacctttat ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt
     2341 ttcttttcca ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc
     2401 gcaaagtcca taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata
     2461 atttcttctt tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta
     2521 gcagatttga taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat
     2581 ttaggaactt tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt
     2641 gtataactct gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc
     2701 cgaaaaaaat tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta
     2761 tcaatttctt tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt
     2821 tttttagaag aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga
     2881 ttgtagacct tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt
     2941 ggatagattc tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt
     3001 atgattgttt ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt
     3061 tgtcagaaga caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct
     3121 tggaaagtca cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt
     3181 caattctgtt ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca
     3241 ctccactaaa gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc
     3301 agatatttca tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata
     3361 aagaagaaat actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
     3421 tatagactga ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt
     3481 cgagttacca acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg
     3541 gcgacttctg cctaattctg ttaaatttaa cacaaaggcg tgattatttt gaaacccaaa
     3601 aagaaaattt atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt
     3661 acaaatacct atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa
     3721 caagcaatta attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg
     3781 gaaaataaca ccgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat
     3841 ggagaaaata cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg
     3901 attaaatatg tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca
     3961 caatttttag caaaagaact tcaaaatcta gtagatagta atataacaga tacaacctta
     4021 ttttttcaag ataatagcat ttattattcc gataatggac aaattaaact actttggaaa
     4081 tcacctttaa gtacagaaaa aaatggtgtt tcttccctta cagaaaaaaa tgatcttata
     4141 tccgtaaata taaaaaataa aattaaaaat ttttctaaaa aacaaaattt tcctgcggtt
     4201 aaacgtgtaa aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca
     4261 gaaacccaaa caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaac
     4321 aaaacagatg tacgtgctac ttcagctaga gccattatgc attataccta tcctaattta
     4381 aacaacagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
     4441 tactgtggtg tttatcctaa aaaaatagca cgaatactaa acctttcaga ggtccaaaca
     4501 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
     4561 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
     4621 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
     4681 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
     4741 tcattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     4801 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     4861 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     4921 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     4981 aagttttcat ttacaaagca acaaataatt tactaacact gcgctcgaat aaaaaacaat
     5041 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     5101 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     5161 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     5221 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg gttttgaggg ggattcaagg
     5281 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     5341 caaatataga gcttgccaag ccctttagtg tgttttgtag aaagggacct tatagtcact
     5401 ttcacattac aaacgatacg tcctatggac gtatcaaaag ggtgggactt atgagcaaat
     5461 taaagcaaaa tttggctagc aatccttcta aaaaatacac ctattgatca gagtcaaaat
     5521 aaatcagttt tcgagtgagc gaatccaaat ttgcaactct gaagctttgc aaatgagtgt
     5581 gccggcttcc gtaaaagtct aggtaacatt agtcgatata tacatagggc tctaatttgc
     5641 gttttaaggc gttttaaacg ttctggtatg taattatacc aaagcgactt taaaacgcct
     5701 taaaacgaat tttagaggtt ctgtagaacg ttttagggtt gttagggctt gccctgaccg
     5761 cctgtaaggc gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg
     5821 tagatgagcg ctcagagctt ctaaaaggac tccttacgaa ctgtttttaa gcaaaggagc
     5881 ctttaggcga ctgcgcagtt taatgtgaga gcgtagcgat cacaccttta aagcgaccgt
     5941 agggagcaga ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa
     6001 aattttaggc gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa
     6061 acctctttac cgtcggctgt atccttactc tcccaaggtg tgcagaatct tttaggggtc
     6121 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     6181 ggggtctttt tgtctgtttt taggggtctt taattagggg gttgaccccc attagaaagc
     6241 tctttataat tttgttaagt agttaatctt gaaggaggat tttatggcaa atgaaattgt
     6301 gaaataccac catgaattaa atacgattcc tttacgcaaa tttacgtcag tagaaatgaa
     6361 tttattcttc tctataatat ctagaatgcg tgatgtaggc gataaaaaag tacaatttac
     6421 attcgaacaa ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga
     6481 tgatttagaa acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga
     6541 cggtttgcaa cgtgaacgat ttgttttatt taatcaattt aaaatcgatg gaaaagctga
     6601 tattcctttt gctgaaatac aagtccatga gaaagcctta cccttactta ataatcttga
     6661 agaatgggtt cgatattctc tccaacaatt caatgaatta gaaagcagtt attccaaaac
     6721 catgtttaga ttattaaaac aatacagaac caaaggaatt gccaattttt caaaagaaga
     6781 ctttcatgaa ttacttgata tccctaaaag ttatagagaa agtgatataa ataaaaaaat
     6841 tataaagcct attcgccaag aactaaccgc aatcttcaaa ggtttgacaa tccaaaagag
     6901 atacggaaaa ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat
     6961 aaaaaaatca gacgacttta acaaacatgc tccaaccaaa gaagaacaaa ataaagcttt
     7021 ggaactagct aatcaacagg ttcaatctga aaaagaacct acttcattac aagaaaaaat
     7081 caaagaacaa aaacgaaaat tagaactttt acaacagtta gcagaattag aaagaaaaca
     7141 aaatacccca agtaagaaaa ttaacaaaca aatagcagat aacaattccg atattacccc
     7201 agaatttaaa gaaaacttac tagataatct ccaaaactta tttaaagaat aaggggtttt
     7261 tatgacagat aaaactatca aagaattagc agatattcta ggtgtttcaa aagataaagt
     7321 gaagtatcaa ctgaggaaat tacctagtaa ttttacctac aaaaaaggaa atattaccta
     7381 cctgaaaaaa gaagcagtaa caacaattac aaaggtttta tcaggtaatg aatcgggtaa
     7441 cttacctagt gaattacccg attcattacc taccgattac ctactcaagg agattgaaac
     7501 taagaataat catattgaag aaaaagatag gcaaattgaa aaacttcaaa tagctttaga
     7561 aacgcagcaa aaattactcg accagcaaca acaactcacc ctacaagcca atcaacaaat
     7621 caaaaaactt cacgaacaac tccagctaac ctatgcagaa gaatcttcag
//
