LOCUS       NZ_CP044277            12527 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Enterococcus faecium strain V2937 plasmid pHVH-V2937-3, complete
            sequence.
ACCESSION   NZ_CP044277
VERSION     NZ_CP044277.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12818041
            Assembly: GCF_008728475.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     The association between vancomycin-resistant Enterococcus faecium
            colonization and infection: A retrospective WGS study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12527)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2019) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard alle 30,
            Hvidovre, Copenhagen 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2019
            Assembly Method                   :: Unicycler v. Version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 05:32:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,940
            CDSs (with protein)               :: 2,940
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 44 of 110
            Pseudo Genes (incomplete)         :: 65 of 110
            Pseudo Genes (internal stop)      :: 22 of 110
            Pseudo Genes (multiple problems)  :: 16 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044277.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Copenhagen University Hospital Hvidovre, Copenhagen,
            Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="V2937"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pHVH-V2937-3"
                     /geo_loc_name="Denmark:Copenhagen"
                     /lat_lon="55.67 N 12.56 E"
                     /collection_date="2018"
                     /collected_by="Copenhagen University Hospital Hvidovre"
     gene            complement(138..1286)
                     /gene="tnpB"
                     /locus_tag="F6440_RS15870"
                     /old_locus_tag="F6440_15870"
     CDS             complement(138..1286)
                     /gene="tnpB"
                     /locus_tag="F6440_RS15870"
                     /old_locus_tag="F6440_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(1303..1707)
                     /gene="tnpA"
                     /locus_tag="F6440_RS15875"
                     /old_locus_tag="F6440_15875"
     CDS             complement(1303..1707)
                     /gene="tnpA"
                     /locus_tag="F6440_RS15875"
                     /old_locus_tag="F6440_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(1793..2119)
                     /locus_tag="F6440_RS15880"
                     /old_locus_tag="F6440_15880"
     CDS             complement(1793..2119)
                     /locus_tag="F6440_RS15880"
                     /old_locus_tag="F6440_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_080440585.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNRIRQKSPILYRWVMNAVRYE
                     GYRW"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2252..2626
                     /locus_tag="F6440_RS15885"
                     /old_locus_tag="F6440_15885"
     CDS             2252..2626
                     /locus_tag="F6440_RS15885"
                     /old_locus_tag="F6440_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            2680..4566
                     /locus_tag="F6440_RS15890"
                     /old_locus_tag="F6440_15890"
     CDS             2680..4566
                     /locus_tag="F6440_RS15890"
                     /old_locus_tag="F6440_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151061531.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQVKSRLLCKMLYNVFTISTYQN"
                     /name="hypothetical protein"
     gene            complement(4550..5722)
                     /locus_tag="F6440_RS15895"
                     /old_locus_tag="F6440_15895"
     CDS             complement(4550..5722)
                     /locus_tag="F6440_RS15895"
                     /old_locus_tag="F6440_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="WP_000195429.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEY
                     IQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKALM
                     ASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPY
                     LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER
                     GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGI
                     FKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLI
                     ERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
                     /name="IS256-like element IS256 family transposase"
     gene            6135..6755
                     /locus_tag="F6440_RS15910"
                     /old_locus_tag="F6440_15910"
     CDS             6135..6755
                     /locus_tag="F6440_RS15910"
                     /old_locus_tag="F6440_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            6869..7006
                     /locus_tag="F6440_RS16290"
     CDS             6869..7006
                     /locus_tag="F6440_RS16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            7448..7815
                     /gene="mobC"
                     /locus_tag="F6440_RS15915"
                     /old_locus_tag="F6440_15915"
                     /pseudo=""
     CDS             7448..7815
                     /gene="mobC"
                     /locus_tag="F6440_RS15915"
                     /old_locus_tag="F6440_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="mobC"
     gene            7797..8711
                     /locus_tag="F6440_RS15920"
                     /old_locus_tag="F6440_15920"
     CDS             7797..8711
                     /locus_tag="F6440_RS15920"
                     /old_locus_tag="F6440_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8671..8949
                     /locus_tag="F6440_RS16600"
                     /old_locus_tag="F6440_15925"
     CDS             8671..8949
                     /locus_tag="F6440_RS16600"
                     /old_locus_tag="F6440_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            8951..9640
                     /locus_tag="F6440_RS15930"
                     /old_locus_tag="F6440_15930"
     CDS             8951..9640
                     /locus_tag="F6440_RS15930"
                     /old_locus_tag="F6440_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(9761..9847)
                     /locus_tag="F6440_RS15935"
                     /old_locus_tag="F6440_15935"
     CDS             complement(9761..9847)
                     /locus_tag="F6440_RS15935"
                     /old_locus_tag="F6440_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            10492..11229
                     /locus_tag="F6440_RS15940"
                     /old_locus_tag="F6440_15940"
     CDS             10492..11229
                     /locus_tag="F6440_RS15940"
                     /old_locus_tag="F6440_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            11257..11796
                     /locus_tag="F6440_RS15945"
                     /old_locus_tag="F6440_15945"
     CDS             11257..11796
                     /locus_tag="F6440_RS15945"
                     /old_locus_tag="F6440_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            11812..11982
                     /locus_tag="F6440_RS16295"
     CDS             11812..11982
                     /locus_tag="F6440_RS16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     BGC             1..7626
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          10493..11229
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7798..8708
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacctgacgg ttcttaacgc ttgaagtggg agcttcttgg gaatagagca tacttgatag
       61 cgtatttctt tactaacagc ggtgtctctt atttaccaag ctatccccgt agttcctacg
      121 gttcttgatg attggatcta agccaatccc atagaatgta gtcttagacc ttcggtcaga
      181 atattgatac tagcattgat atcacgatca tgatgagtat gacaaacagg acaagtccat
      241 tctcgaattt caagagattt cttgccatct ttatgtccgc actctgaaca aatctgacta
      301 gacggaaacc atttatctac tttgatgatt tttcgtccat accagtcagc cttgtattgt
      361 aatttagtca caaactttga ccaagaaaca tcagaaatac ttttagctaa tttatgattt
      421 cgcaacatac cttttgtgtt taagtcttca atacagataa tatcgtgatt tttgatcatt
      481 tctgtactca acttattcag aaaatcagta cgttgattca ttactttttc atgcaatcta
      541 gctactttgc gtttttgttt ttgataattt ttagcttcaa atagattggt acctttattt
      601 ttagccaata aagcacgcct agacaatttg cgttgttcac gctttagttt cttttccatt
      661 ttggacgtga atttattatt atcaattttt tgtccatcag aaagaatcgc aaagtccata
      721 atacctaagt caataccaat tgtggaatta gttttaggta attcgataat ttcttcttta
      781 cacaacaaag aaatatagta tttaccgcta gaatgacgtg atattgtagc agatttgata
      841 atcccttttg gttgtctatg aagcttaatt cttactaatg acttcaattt aggaactttg
      901 atgaatttac tatcaatcaa agcaactgta ccattttgat tattcgttgt ataactctga
      961 acaggatttt tcttactttt gaaacgtgga aatccaacgg atttatcccg aaaaaaattc
     1021 ttgtatgctt tatctaaatt aagttgggca ttggctaaag caaggctatc aatttctttc
     1081 aaaaacggaa attctttctt gtatttagct ggtgtcggaa aagtcatttt tttagaagaa
     1141 tcatttttaa cttcttcata cgcctttttt cggtcatcaa gcattagatt gtagacctta
     1201 cggacacaac caaaagattt ggcaaagaag atttcttgtt cctctgttgg atagattctg
     1261 aatttgtatg cttttagttg ttccataaag ttcacctact ttctatttat gattgtttcc
     1321 ttgattttgg atatacttct taatcacatc aattggtgcg ccaccagttg tcagaagaca
     1381 aaaactttta gaccagaaca tttctttcca aagaaattgt ttgactcttg gaaagtcacg
     1441 ttttatcaat cttgagctgg cacttttata ggcattgatg aattttgtca attctgtttt
     1501 aggttgagct ttgaacataa tatgaacgtg gtctttatca tgattccact ccactaaagt
     1561 aatgtgatac gattctgaaa ttctttcaaa agtagttttg gcataatcag atatttcatc
     1621 atcaatcact tgtctacgat atttcgttac taaaacaagg tggtaataaa gaagaaatac
     1681 tgaatgatta tttgtatcta attccatgtt atatcaactc atttcttgta tagactgatt
     1741 gtaacatgga acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac
     1801 ggtaaccctc ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc
     1861 taattctgtt aaaaataata gctgtcaata ccagtaaatt atttttttta ttttttgaat
     1921 tatcttcatt taaaatagaa tttatgttag tactataaaa ttcacaaatt ttttttagtg
     1981 tctctatatc tggtgttgtt ttgttatttt cccactttga aatagtttga cgtgtaacgt
     2041 taaattttct tgccacatat tcttgagtat accctttttt cactcttaat tctcttagct
     2101 tagatcctaa atcctccatt ttttctccta tgtaaacgat tatttacata aaattttatc
     2161 ataaaaatac taacgcgcca aaaaagttct ttattttata aaacacacta aaataaaata
     2221 attctagtaa aaaagatagg agaaataagt tatgaaaaaa tttttatgtc tttctgcttt
     2281 aatgggtgtc ttattaacta gtggaggaat agctagtgcc acagaagctt taaatctaga
     2341 tgtacccgaa gaccataccg ccatttacgg aggaggaatg gaagctacag aagaaggatt
     2401 ttcttctagt aaattgagat atgctgctgg cggcggggat ttcaactgtg gggttaatgg
     2461 atttaaagtt tatgcaaatt actaccatgc tagagctaaa catagtgcaa cagctaaaaa
     2521 tggacgaggc ggtcaagtac gatcagttca gaaagctgga gtaagagctt atgcaacatg
     2581 caatgccaca cttactggaa atactggttg gtggaatgtt tattaaaaaa gagagtttaa
     2641 actctctttt ttatttacat ttgaaaggaa attcaaaata tgttaaaaaa aataaacgct
     2701 gttttatcac tttttgtctg cattacaata ctattttttt ctactaatgt tataaatggt
     2761 ataaagactt ctgaagcgct atataaaaat accgctgaaa ttcatatgca atataaagat
     2821 gatatggact ttaacagtta tataaacgct ttgtatgaaa tatctacaca agaaaatatt
     2881 aatatttctc aatatagttt tactgctgtt aaccaactaa ctattcttgc tacaaaccct
     2941 aacgctaaca aacactggaa attttctaaa aaaaatatat tagcaagaaa cgattctaaa
     3001 gtccattata ctaataacaa aaattctaag taccaattaa aacttccaaa taatttttta
     3061 aatataaata tttatccttt ttctcatgtg gaaaatattg gattaagtga aatattatat
     3121 gtacaaggaa actcaaacaa attaattcct gtactgaaaa aatatggaac acctactatt
     3181 tccaatatca atcaatcaga tacttttcaa attaatacta atattgttct tgttatctgc
     3241 tatcttagta tatttattgt aattactaca attatatttg ctttttcaaa aatgaaagaa
     3301 atcactctaa aaaaaatgtt aggctataac tcttttgata ctataaaaga ttctattaaa
     3361 gagttaaacc tcttattttg ttctttgatt ataactttca ttttatctat ttttttgagc
     3421 atatattaca gtaacgaaaa ttttttccta tcatttcttc tatgtagtat ttttcatttt
     3481 ataattagtt ttttatttat catagttact ctaagtatct atcatttatc tagtataaaa
     3541 aattatctaa aaaattcacg accacttaag ttaaatcttt gtatattaaa tatctgtcta
     3601 ttcttaagca tgatccttct gttaatagct tctacaaaag taataaatac ccataatgaa
     3661 gccgaaaata attctcttaa atattgggaa agaactacta atttatacaa aacaaatatt
     3721 actaatcaat taaatagaaa caacaccgta gaagaaaata actacttgaa aaaagctagt
     3781 aaatttgttt ataaaataca gaagaattat aaaactttta ttatcgcgcc atacaattac
     3841 gcaactatcc aagagaataa taaagaattt tttattggac aaaaagttta tccaaatttt
     3901 gaagactatg ttagtcaacc tgctggtgca ggaatatgtg ttgatttaaa ctatctaaaa
     3961 tattacaatc ctatttcttt tgaagaatct actgacttaa atttaataaa ctctgatcca
     4021 ctaactactt acattctagt acctaaaaaa tataaagaat atgcaaaaat gattgaaaaa
     4081 aattatttag aagacataca gtttagactt gaggactctc ctcctcaagc tccttataaa
     4141 aatcctaatt tgaaaaattt aaaaataaaa ctaatatttg ttaataataa ccaaaagtat
     4201 tttagtttta ataccttata tggaaaggca aaagataact atacaataac tgatcccata
     4261 gttagagtta ttaatccaga aataacagaa tcattatttt ggggaaatat attgactagt
     4321 gatggtggat tattttttga tcagaaacat acttcaaacc aaaacttttt taatcaaatt
     4381 aatccgtata taaaagaatt taacctagaa aatatcataa atttttctta ttctgttttt
     4441 caagaaactg gtgaattatt atctcaaaaa aaagtaaata aattaaatgc atatctacaa
     4501 gtcaagtcca gactcctgtg taaaatgcta tacaatgttt ttaccatttc tacttatcaa
     4561 aattgatgta ttttcttgaa gaataaatcc attcatcatg taggtccata agaacggctc
     4621 caattaagcg attggctgat gtttgattgg ggaagatgcg aataatcttt tctcttctgc
     4681 gtacttcttg attcagtcgt tcaattagat tggtactctt tagtcgattg tgggaatttc
     4741 cttgtacggt atattgaaag gcgtcttcga atccatcatc caatgatgcg caagcttttg
     4801 aatattttgg ttgatcgata taatcatgaa tcaatcgatt tttagcctca cgcgctaagt
     4861 taatatctgt gaacttaaaa attcctttaa cagcttctct gaaagatttt gaattttttt
     4921 taggaatggt ggtaaagata tttcttagga agtgaacttg gcatctttgc caacttacgt
     4981 tggtgaagga ttttctaatg gcagagacta atcctttgtg cgcatcagaa ataacgagtt
     5041 ccgtaccttg taaaccgcgt tcttttaggt attcaaaaaa tgttgtccag gtctcttcgc
     5101 tttcgccact ttgaatcatg aagccgataa tttcacggtc gccatctttg gttattccaa
     5161 tcgctatatg acagcttttt gagagtactc gattttcttc tcgtactttt atatagagta
     5221 catcggtcat taagtaagga taattttttt ctgataataa acgattctgc cactcgttaa
     5281 ccataggttc tagctgttct gttaagctag aaacgaagga cttagagacg gatttaccac
     5341 aaagttcttc cacaattttt gatactttac gagttgaaac gcctgataca tacatttcca
     5401 acattgaagc catgagggct ttttcgtttc gttgataacg ttcaaacact gtgggtgaaa
     5461 aatggccatc acgtgttctg ggtactttta attctagcgt gcctacacgt gtcgtaaagc
     5521 tgcgctcata atagccattt cgttgacttt gtcggttttc tgttcgttca tattcttttg
     5581 cttgaatata ttctgttcgt tgattttcca ttagttgatt aaataccgtt gttaaaatat
     5641 ttttagaaac gtcatccttt acagaatatt caataatgct ttgaatctct tcgcttttca
     5701 gtgtaaaatg tacttgggtc atgtaaaagt cctcctgggt atgtttttgt cgttaaaaac
     5761 attgtaccgt aaaaggactg ttatatggcc tttttacttt tacacaatta tacggacttt
     5821 atctatctac aatatctttt aatattagga ttatttataa taattaattt tcaaatgatt
     5881 tttgttagtt ttactataaa ttcaaaaaaa atagctttaa aaaaaatatt tggatattca
     5941 ccaataagtc aagttttaga tttatgttta ctccctttta caatagagtt atttgctact
     6001 atgtgttttc aattgatatt ttcaaaaaac acttacttgt attttataat ttttatgatc
     6061 tttttaataa atctaattac ttctattatt atttataaaa aaaatagttt taaatatttt
     6121 aaggagaata ttttatgata ttatcagttg agaatttcac cctgtcaatt aataaaaaaa
     6181 agatcaaaca aaattggaac ttttcaatat cagaaaaaga ttttattata gtaacaggaa
     6241 aaagtggtag tggaaaaact actctgttaa ataatttaag cctaatagat aaacaatata
     6301 ccggtaactt attctatttt gaaaagaaag tttctaaaca tgatatacaa cgtctacgaa
     6361 gaaatcatat tagctattta tttcaaaact atgctcttct tgaaaatcaa acagtacaat
     6421 acaatttcga attagcaaaa aagtttaaca gtaatatttc caatgatcat atttataatt
     6481 tattaacaga tttttctttg ccaaaagata tcttaaaaga aaaaatcttt ctcttaagcg
     6541 gtggagaaca acaaagaata gcattaatta gaaatatgtt aaaaccattt gatatattat
     6601 ttgcagatga acctacagga aatttagata aagaaaactc aacttttata atgaactttc
     6661 tgaaaaattt aacagaaaaa gaaaaaaaag ccgttgtact agtaacacat gacttaaatg
     6721 ttctaaacta tggaactaat attatagaat tataattttt acccgtgatg ctagctgatt
     6781 taaaactagc accacgggta tttattttat attccatctc tcctatccca aaatttaaat
     6841 aatcacttaa taaaaaggag gcaccctagt gattttattg aaaaaagagt atgaaaaatt
     6901 ttatgataga cttatttatt ctttgcaatt gccaaaaaaa ttaaaccgtc aattacaaaa
     6961 gacttatgaa actaaatcaa ctgaagaatg ggagagttat tgctagattt ttggtagaag
     7021 taacaaaaag tggacatatt atcataatga ctcgccgaaa gtctgataaa aaattggcta
     7081 aaaatattgc tgaatgttta ataaaactat cagttttagc taaatagtta gtagtttaca
     7141 caaaaaagca tttatgtttt ttgttgcaaa gtaacaattt catcttcggt gaaataagcg
     7201 aaactttaca aaaagtggct agcatttgct agcttttttt gtattgtgga gcgtggcttt
     7261 tgagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg cactaaatac
     7321 gtaggtattt ggctagcaaa tatagagctt gccaaaccct tttagtgagc tttactcatt
     7381 gtaccttttt aggtacaatg gtattgcgaa cgatacgtcc gtaggacgta ctgaaagggt
     7441 gggacttatg agcgaacaaa atcaaaattt ggctagcaat tcttctaaaa aatataccta
     7501 tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa tccgaatttg caactctgaa
     7561 gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg
     7621 acaccgaaag tggcgccaga cattgcccag gcaatcgcgc gcgatttagc caaagctgga
     7681 gggaatatca atcaaattgc taagtggtgc aatacacacc aacatgacgt tgcttctggt
     7741 gacgctcaac gtttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg
     7801 caacaattaa ggtaagccga accacttctt gtagtcgtgc aattaattac gcagaaccac
     7861 gagcaaccat taaaacagga ataaactgtg acgtcgatta tgccaaaagt gaaatgaaac
     7921 aaattcgcat gctgtatgga aaagacgacc gtgtacaggc acacttattg attcaatctt
     7981 ttcgaccagg agaaattacc gctgaaaaag ctaatcaact aggggcagag tatgcaaaaa
     8041 aaatagcccc cgatcaccaa acagctattt acacacatac agacaaagac catattcata
     8101 atcacattgt cattaactcc gttaatctgg agactggaaa aaaatttcaa gctcatggtc
     8161 aagcatttct aaacaaatgt tatgacctaa atgacgagat ttgtcttgca cataacttac
     8221 caatcactga gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata aaattaaaag
     8281 aaaaaaatga acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa acaatgaaaa
     8341 acaaaaaaac cagaacctat gaccaatttt gtaattccct caaaacattg gggattcatt
     8401 gcttcaatcg cgggaaaaat ttcacttacg aattgatcca caaaaagaaa aaagtacgat
     8461 ccaacagact aggaaaggac tacgaaaagg agacgatttt acatgagctg gacagacgag
     8521 aacgaaccta taaccaacag cgaaccaacg agctacgaag agctcgagct agcattcgcg
     8581 aaactgcaga agaagtacga agagaacaac gaactcttac gcctgaacaa ccggaaatta
     8641 gaccggctcg aagaaaccat gaacaacacg ttgaaagagg aacagaccac caacaagaat
     8701 tatcgctcta aaacgttaaa agacatagaa aacgaaataa agaaacaacg tgcttttctc
     8761 gttccttata cgcaagaaag gatacaaact acgattgatt tacaagttaa aaaactccat
     8821 caagagctat tagaccagcg aaataaggaa cagaaaaagg tgcttattca acgatcattg
     8881 gattttcttg cccccttatg ttgcttagtc ctgacactgt tcgcattttg gttagtatgg
     8941 ggaggctaaa atggcatttt atttacccta tctactcatt tttgttagta tttcaggaag
     9001 tatttggctc atctataaaa tttttcaaac aagatattca ttaaaaggct ctaaaattcg
     9061 atttaagcga tttttctttc ttggctgtat attttccttg ataatcgttt caagcggctt
     9121 attcggcgtt ttagagggaa ataagcgcgt ttctagaagt attttattag gaaatgtcac
     9181 tcaaaagtat gaatcagcgc gtgataaaaa gaaaaaagag caagcattag cacaaaaaat
     9241 ggaagagttt accacttgtt atgaggaaat gaacgatatt tttgtgaatc aagaaaaacg
     9301 tttgacggat aaaaacatgg aaaaactcac tcgtctttat caaaacttgc cagaaaaaca
     9361 gcaaaaagag gttcaagaca actatgagcg aaccaaaaaa gacgtgcaat atgtaaaaga
     9421 tacaaagata gaagaagcct gtagtgatct atttggagac acaaaccctt ggtttgccag
     9481 tgaagaagaa aaaaaagaaa aacaacaatc tgtcacatat gaacgctatg aaatcttact
     9541 tcaacaagca actaacatgc aaaatccgac aaaaaaagaa accgccttaa actatctaaa
     9601 aagtgtcaag gaatggttag atcagcaaca acaaaattga atttgattta aaatcatgtt
     9661 aaactgaatg tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc
     9721 tctggggctt tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat
     9781 tcaaccaata actgaataaa gagacacaaa ctcctacaag aagggggcaa atgaacagtt
     9841 caaacattag atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat
     9901 atcttaaaaa attaaatttg tgtggtgttt ttaagtatca cttgttttag ggttgttagg
     9961 gcttgccctg accgcctgta aggcgcttct cgactagatt aagggaaatt aatcaagaat
    10021 tgtcttagaa cggcttagac aagctttcag agtctcacac ctcctctttg aacaacggtt
    10081 ttagcaaagg agccggaggc gactgcgcag tataatatga gagcatagcg atcacacctt
    10141 tttagcgacc gtagggagca agttttccag tttagaaaaa ttcgcgaacc tttttggagg
    10201 aaaaagtagg agttttttag gcgggaaatt caatgtgctc ttaagatata ctgttagata
    10261 tactgttaga tatactgtta ataataactg ttagggatta gcaaccttat gggagagtaa
    10321 gtattgacat aggtagtggg ggataaattg tcacactatg ggggataaat tgtcacacta
    10381 tgggggataa attgtcacac tatgggggat aaattgtcac actatggggg ataaattgtg
    10441 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
    10501 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
    10561 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
    10621 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
    10681 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
    10741 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
    10801 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
    10861 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
    10921 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
    10981 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
    11041 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
    11101 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
    11161 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
    11221 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
    11281 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
    11341 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
    11401 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
    11461 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
    11521 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
    11581 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
    11641 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
    11701 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
    11761 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
    11821 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
    11881 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
    11941 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
    12001 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
    12061 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
    12121 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
    12181 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
    12241 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
    12301 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
    12361 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
    12421 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
    12481 aaacctaata tagtaacaaa tatagtgaac tactcaccac ttagcta
//
