LOCUS       NZ_CP044267            13703 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Enterococcus faecium strain V1836 plasmid pHVH-V1836-3, complete
            sequence.
ACCESSION   NZ_CP044267
VERSION     NZ_CP044267.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12818007
            Assembly: GCF_008728455.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     The association between vancomycin-resistant Enterococcus faecium
            colonization and infection: A retrospective WGS study
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13703)
  AUTHORS   Rubin,I.M.C., Pedersen,M.S., Mollerup,S., Kaya,H., Petersen,A.M.,
            Westh,H. and Pinholt,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2019) Department of Clinical Microbiology,
            Copenhagen University Hospital Hvidovre, Kettegaard alle 30,
            Hvidovre, Copenhagen 2650, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2019
            Assembly Method                   :: Unicycler v. Version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 05:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,287
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 3,028
            CDSs (with protein)               :: 3,028
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 82 of 170
            Pseudo Genes (incomplete)         :: 89 of 170
            Pseudo Genes (internal stop)      :: 41 of 170
            Pseudo Genes (multiple problems)  :: 34 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044267.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Copenhagen University Hospital Hvidovre, Copenhagen,
            Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="V1836"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pHVH-V1836-3"
                     /geo_loc_name="Denmark:Copenhagen"
                     /lat_lon="55.67 N 12.56 E"
                     /collection_date="2017"
                     /collected_by="Copenhagen University Hospital Hvidovre"
     gene            complement(join(13474..13703,1..223))
                     /locus_tag="F6439_RS16385"
                     /old_locus_tag="F6439_16385"
     CDS             complement(join(13474..13703,1..223))
                     /locus_tag="F6439_RS16385"
                     /old_locus_tag="F6439_16385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_060805110.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLESI
                     TKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVNVI
                     IHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
                     /name="PTS sugar transporter subunit IIA"
     gene            complement(210..860)
                     /locus_tag="F6439_RS16390"
                     /old_locus_tag="F6439_16390"
     CDS             complement(210..860)
                     /locus_tag="F6439_RS16390"
                     /old_locus_tag="F6439_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="WP_060805109.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNNDD
                     FARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKERGV
                     VTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTIDNI
                     AAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
                     /name="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
     gene            complement(838..2949)
                     /locus_tag="F6439_RS16395"
                     /old_locus_tag="F6439_16395"
     CDS             complement(838..2949)
                     /locus_tag="F6439_RS16395"
                     /old_locus_tag="F6439_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012579.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_060805108.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDEL
                     SLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRYSE
                     LLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIESSMA
                     KLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLTKLYY
                     LIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTLLFLSF
                     NTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYRIRYELP
                     FEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCSELLNSSI
                     IETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVEDVDLIFSNQ
                     PLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRYSAVQNEESL
                     QTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTWQNAMELSCQP
                     LLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGCSLLISVDPIIF
                     PGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTEDEVRKKIKNFLRR
                     KQYVNNEFS"
                     /name="PTS sugar transporter subunit IIA"
     gene            3118..3303
                     /locus_tag="F6439_RS17155"
     CDS             3118..3303
                     /locus_tag="F6439_RS17155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060805107.1"
                     /translation="MYKLQTTVQLAFEDFNQPIGLKMNPSNRWIQKAELIPWAELEKDY
                     AKNFRKKKETSQNPFA"
                     /name="hypothetical protein"
     gene            3300..3389
                     /locus_tag="F6439_RS17160"
     CDS             3300..3389
                     /locus_tag="F6439_RS17160"
                     /inference="COORDINATES: protein motif:HMM:NF017416.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228780288.1"
                     /translation="MTFGALLIQKEYSYSDEETVLQIQENPYL"
                     /name="hypothetical protein"
     gene            3479..3709
                     /locus_tag="F6439_RS17165"
     CDS             3479..3709
                     /locus_tag="F6439_RS17165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224752214.1"
                     /translation="MIEINEKILVFDKKKDDKDNDSYDDFNSGTLILDATCAPQNIKYP
                     TDTELMNDTRTHAEKIIDVVCEGKKLKKPVL"
                     /name="hypothetical protein"
     gene            3824..4153
                     /locus_tag="F6439_RS16405"
                     /old_locus_tag="F6439_16405"
     CDS             3824..4153
                     /locus_tag="F6439_RS16405"
                     /old_locus_tag="F6439_16405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_060805105.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGRM
                     ANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLYKRFCCKVLNLL
                     SNKVE"
                     /name="transposase"
     gene            4181..4861
                     /locus_tag="F6439_RS16410"
                     /old_locus_tag="F6439_16410"
     CDS             4181..4861
                     /locus_tag="F6439_RS16410"
                     /old_locus_tag="F6439_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_060804980.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKQLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEDILGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(4891..6306)
                     /locus_tag="F6439_RS16415"
                     /old_locus_tag="F6439_16415"
     CDS             complement(4891..6306)
                     /locus_tag="F6439_RS16415"
                     /old_locus_tag="F6439_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434062.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="WP_060804982.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAGL
                     ENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWFQR
                     AVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINLIAN
                     TSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHWTIFG
                     LSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVITFIIIA
                     PITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLMANGGDY
                     IWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVNLKYKFPF
                     VVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASCVVSFGATY
                     LYGKNKVLLQSFK"
                     /name="PTS transporter subunit EIIC"
     gene            complement(6299..6601)
                     /locus_tag="F6439_RS16420"
                     /old_locus_tag="F6439_16420"
     CDS             complement(6299..6601)
                     /locus_tag="F6439_RS16420"
                     /old_locus_tag="F6439_16420"
                     /inference="COORDINATES: protein motif:HMM:NF025668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="WP_169038116.1"
                     /translation="MTTTVSNKGSRDGYEVVQLYIGAQYGRYIRPTKDLVAYKKVFVKS
                     GDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQALTNKEEIEVRINE"
                     /name="fibronectin type III-like domain-contianing protein"
     gene            complement(7754..7903)
                     /locus_tag="F6439_RS16430"
                     /old_locus_tag="F6439_16430"
     CDS             complement(7754..7903)
                     /locus_tag="F6439_RS16430"
                     /old_locus_tag="F6439_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="WP_074400109.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLE
                     NELR"
                     /name="type I restriction endonuclease subunit S"
     gene            7986..8667
                     /locus_tag="F6439_RS16440"
                     /old_locus_tag="F6439_16440"
     CDS             join(7986..8177,8179..8667)
                     /locus_tag="F6439_RS16440"
                     /old_locus_tag="F6439_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="WP_002319817.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCHT
                     TIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLN
                     NLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLM
                     GITA"
                     /name="IS6-like element ISS1N family transposase"
     gene            <8727..9113
                     /locus_tag="F6439_RS16445"
                     /old_locus_tag="F6439_16445"
                     /pseudo=""
     CDS             <8727..9113
                     /locus_tag="F6439_RS16445"
                     /old_locus_tag="F6439_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(9782..10684)
                     /locus_tag="F6439_RS16450"
                     /old_locus_tag="F6439_16450"
     CDS             complement(9782..10684)
                     /locus_tag="F6439_RS16450"
                     /old_locus_tag="F6439_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="WP_080441793.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHSI
                     AEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLTTL
                     NDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGIPII
                     THCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHIGREL
                     ANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEELLKVM
                     SEPTFNKMIEMNPINILSK"
                     /name="aryldialkylphosphatase"
     gene            complement(10696..11844)
                     /gene="dgoD"
                     /locus_tag="F6439_RS16455"
                     /old_locus_tag="F6439_16455"
     CDS             complement(10696..11844)
                     /gene="dgoD"
                     /locus_tag="F6439_RS16455"
                     /old_locus_tag="F6439_16455"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /GO_function="GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="WP_060805113.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAGA
                     YEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPVYE
                     LLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKVAEV
                     VERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEEHFSE
                     VANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAEAYDIA
                     VAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQYKDGFV
                     DLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
                     /name="dgoD"
     gene            complement(11863..13140)
                     /locus_tag="F6439_RS16460"
                     /old_locus_tag="F6439_16460"
     CDS             complement(11863..13140)
                     /locus_tag="F6439_RS16460"
                     /old_locus_tag="F6439_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743177.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="WP_151076511.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALTV
                     GIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMVFI
                     ILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIISFKL
                     ADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQERFG
                     FVGEPMFMGFVIGLVIGLLAKEPVDKVLILGMSTSATMVLTPKMMQILMEGLLPFANSV
                     KKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLPISDIAY
                     QAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQLPAGMTQ
                     ISTEDAGTHIIGFILMKIAEFISNIF"
                     /name="PTS transporter subunit IIC"
     gene            complement(13152..13457)
                     /locus_tag="F6439_RS16465"
                     /old_locus_tag="F6439_16465"
     CDS             complement(13152..13457)
                     /locus_tag="F6439_RS16465"
                     /old_locus_tag="F6439_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="WP_060805111.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFSE
                     VAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
                     /name="PTS lactose transporter subunit IIB"
ORIGIN
        1 catttgataa ctgataatct gtatgtggaa ttgcgattga gcgttcccca atatatagcc
       61 ccgttggata agttgtttct ctcttagtga ttgactcgag atatccctcc tttaccatgt
      121 tttccgtcat cagtttcttt gtaatctttt gaaagtattc ttcttggttt gaaaaacttt
      181 gatttaacaa aatatttttt tcttctaatt taatgagact catcttcttc acttcctgct
      241 tcagctagtt tatctaaata tgattgaatt gattttcgaa tagcttgccc attgcggttt
      301 tgtatatcag ctttttgaaa catgcttgat cctaccccaa agtacacaac tcctgctttt
      361 ttaaaagccg caatattatc aatcgtcact cctccaactg ccattaaagg ccgtatacca
      421 aaaggaccgg ccagttgttt aaagtaggtt ggccctacag tgactgctgg aaaaaccttt
      481 acaatatcgg caccttcatc aaacataact tgaatttccg atggcgtaaa agctgatggc
      541 acagttacca ccccacgttc tttagccaac gaaaaaattg gtgttgaaaa tttttttggt
      601 cctaataaaa atttcgcacc accagcaatt gcttgatctg cttgtgaaaa tgttaaaact
      661 gtccctgcac caatcaataa ttgttgccca aattttttgt tgccttctgt gatgatacgt
      721 gcaaagtcat cattattggt tgttacttca attcctaccg aagtgttcat cgcagccaat
      781 tcttccatga caaacatcgc ctcttcaaaa ctatatcctc ttaaaatgat ggttaattca
      841 ggaaaattcg ttattgacat attgcttcct ccttaaaaaa ttttttattt tttttctcac
      901 ttcatcttcc gtcgtgcaaa gaagtatttc ttgctgatat tcctcgtcct tagaaagttg
      961 tagtaattgg ttaatcgcaa gtaaataatt ttccttcttt ccaattgcaa aaggggaaat
     1021 aaaatggata ggcctatttc caggaaaaat aattggatca actgatataa gtaacgagca
     1081 accgccctta attaccccat tttctggcga tgaatgtgga atagcaatac gattattaag
     1141 aaaataataa gcctcttcat ccgccatttg actcattaaa atagtctgat agttctgatt
     1201 aattacctgc tcctttaata aaggctgaca agacaattcc atcgcgttct gccaagtaag
     1261 ttgtttcgta gatacaagaa taatcccttc agaaacaagc aattccacaa aagatatctt
     1321 ttgtgcctgt ttactttgac gatctagata tattagtgaa tttgcataat taattaaaaa
     1381 tctctcaata tcagtttgta gactttcttc attttgaaca gcagagtaac gcttaataat
     1441 ttcagtaagg ttcctagtct ctttgctgat attattcgta gaaaaatcaa tgagtacacg
     1501 gttccgcaag cggttttttt cttcttcgtt cataatcggt tgtataatat ataatggaat
     1561 ttcacttttc aatggttgat tgctaaaaat caaatcaaca tcttcgacaa acttatccat
     1621 ctctctgacc gaagaactag caacaaactg aatatctgga aaaatttctt tcaatgtttt
     1681 cgccattaca ttggccataa tcaatccatt gctacatacg acgattgcct tttttctaac
     1741 aggtgtttcg ataatagaag aatttaatag ctccgaacaa aaataaacag aaatcaaata
     1801 taattcagtt tcaggaatcg tcttacgcaa aaatgcttcc aaaggtacaa tggattcttt
     1861 aacaaaatta agtaccgcac taacttcatg gttttcctta acaatttcag ctaaatcatc
     1921 tatttcaaat ggtaattcgt agcgaattcg atagattgca ggtcgtaaat gattcatcaa
     1981 tcgattggtc aactcttctt tattactaat ccgaatagct gatttacttt gaaataaggt
     2041 aatcatctcc ataattactt gatagattct tgaatctaaa atttcaaact catttgaaat
     2101 tgtattgaat gataaaaaca gaagcgtcaa ccattcataa tctatagcta tctcagcatg
     2161 ttgataatgt tgatcaatgc agcttcgaat catttgaaac tcatctaatt cctgtaaatc
     2221 ataactaaaa tcaatctgtc ctttcgtcct tcttttaaaa ctagaaagtg ataaacctat
     2281 caaataataa agtttggtta gtgaattatc agaaaatttc aatttaaaag tttgttcgta
     2341 ggtgtgaata aaaaaaccaa caaattccct ttcttcagaa aagagtgact caaaacggtt
     2401 aactgttgca tgcctttcaa ataaataatt gaaaacttct aagagcaatg tatgaagctt
     2461 tgccatgctt gattcaatca tatagccatt ttggggatca aatatcaaag tgtagtctgg
     2521 tggcaactct ttttttattt tagaaaagtc gtttctagcc gtatttttac tgataccaat
     2581 aaacgccatt atttcttggg ttgtcacttt tttggggttg gctagaataa ataacagttc
     2641 cgaatatctt cttatctcac tataagccaa atcatcgttt atatattcag gaatcgcacc
     2701 ttctttaaaa accattgctt gattcatgta tatcaaaagt ttttgaagat cattggcttc
     2761 atataaccac tcccctgagt tacttcgctt tatcagactc aatccatgaa gggataattc
     2821 atcattaatt ttttttatag agtaagttat ctgcctagtc gaaaaatgta ctaatgattt
     2881 caattgttga gagtttgtca catgtgatcg tacaatggtt tctaacagtt ttaaatcttt
     2941 tgtattcaca ttcatctacc tccctgagtt aaatataacc gcttacagta ttaattggaa
     3001 tatccaaaat ttgtcaaacc ttggcttaaa tcagacaaat gacaggcact tctttaattt
     3061 tttatcttat gcaccatcag aatggtgtgg agtaccactc aattttggag gcttttcatg
     3121 tataaattac aaacaaccgt tcaattggct tttgaagatt tcaatcaacc gataggtctc
     3181 aaaatgaatc cgtctaaccg ttggattcaa aaagcagagc tcattccatg ggctgaactt
     3241 gaaaaagact acgctaaaaa ttttcgtaaa aaaaaggaaa cgtcgcaaaa ccctttcgca
     3301 tgaccttcgg agctctactg attcaaaagg aatatagcta tagcgacgaa gaaacggtct
     3361 tacaaattca agaaaacccg tatttatagt tcttcatcgg tttgccgggc tatcaagatg
     3421 ccaagccttt cgatgctcta caatggtcta tttccggaaa cgcttggacg aaacaacgtt
     3481 gatagaaatc aatgaaaaaa tactcgtctt cgacaagaaa aaagacgata aggacaacga
     3541 ttcttatgac gatttcaatt ccggaacatt gattttggat gccacttgtg ctccacaaaa
     3601 catcaaatat ccaaccgata cagaattaat gaatgatacc cgtacccatg ccgagaaaat
     3661 catcgatgtt gtttgtgaag ggaaaaaact gaaaaagccg gttctataat atttggtttt
     3721 gttactcaaa ctgagtagtg acgccaaata tttttttata acaaaaagac atctggttgt
     3781 cctataatta aatcggcaaa aacaatcata ggaggataac caaatgtcca taactaagaa
     3841 tatcaaatgt acggatgaaa agacaagtca ttccatcaaa caaattttga acatcaaaga
     3901 ttcccatatt accttttcaa aagatgccat tagtaaagag cgcgttcatg gccgtatggc
     3961 caatgtattt accggcttat taactggaga agcgcatcgt tgcgaatgtt gtgggtttga
     4021 ttctgtcatc cgtcatgctt atcatgatag ttggattcag ttgcttccgt atcaagaagt
     4081 accaacttat ctacacctgt acaaacggtt ctgttgcaaa gttttaaatc tactatcaaa
     4141 taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt ttaaaggaaa
     4201 gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt ataaccttag
     4261 ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc atacgacgat
     4321 ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa agaaaaataa
     4381 aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag gaaaatggca
     4441 ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt tacgtaaaaa
     4501 acgggacaca caagcagcct atgcttttct taagcagtta gtgaagcagt ttgatgaacc
     4561 gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga aactaaaaga
     4621 atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata atttgattga
     4681 acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac gcactgcctc
     4741 taccacgatt aaaggcatgg aagacattct aggattatat aagaaaaccc gaaaagaagg
     4801 cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta
     4861 aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca gaaccttatt
     4921 tttcccgtaa aggtaagttg ctccaaatga gacgacacaa cttgctagca tacaaataaa
     4981 gaatattggc cagaattcaa acggaataga caagaaacct ggaattccac tgataccaac
     5041 agaagttgca atgacgtgtt gtgacgccat aaatcctcca attattccag aggtcagaat
     5101 ggaaaccacg aatgggaatt tatactttag attaaccccg aacattgctg gctcagtgat
     5161 acccatataa gcagacaaac cggcagccag tccaacacct ttcaactttt tattttggat
     5221 taagaaattc attgcaatag cagcagaacc ttgtgccaaa cttgacatga ctccaatagg
     5281 ccaaatataa tctccaccgt tcgccatcaa ttgaatatga actggtaaaa acgcatgatg
     5341 catccctgtc acgactaatg gcgcaagacc aataccataa gcaaatccgc caataaatgg
     5401 aactttttgg aataaccata caatacctgc gacaatcgta ttaccaatag caattgtaat
     5461 cggcgctata ataataaatg ttataacact agtaaaaaga attgcaactg gtgcaacaac
     5521 caacagttgg attgtttcag gcacacgctt tttcaagaat agctctgttt tagctaacac
     5581 atagccggaa actagagctg gcaatacttg tgcttgtagt cctatcttag agatggacaa
     5641 tccaaagatt gtccaatgtg gaatatctcc cgtggttact gcttgcccgt aattccaggc
     5701 attcatcaaa tctgggtgta caaagactag acctaacaca atcccaagaa gaggatttcc
     5761 tttaaagtat tttacagttg accatgttat taacgcaggc agaaattgaa aggaagtgtt
     5821 tgcgataaga tttatcatat cagcgacatc agcaatatta ggatatactt caatgagtga
     5881 ctgctcaaaa aagattcctg ggtttgttaa taaattattc agtcccatca gtaaaccagc
     5941 tgtaacgatc gctggtaaaa tcggcacaaa tacatcaccc aatattttaa ctgcacgttg
     6001 aaaccaattc aaattcaact cagccgctac ttttttaacg tcttctttgt ttgccccact
     6061 cagcccagat tggcttataa cctcttcata gatatctgct actttaggac cgataacaac
     6121 ttgaaactgt ccattagcat tgaaggtacc cttcacatct tccaggtttt ccaatcctgc
     6181 cttatcaact ttagtgtcat ctcgcaaggc aaacctcaga cgtgtcacac agtgagtcag
     6241 tgctgcaata ttctcttttc caccaacaaa gttaatgatt gatttagctt cttctttatt
     6301 actcattaat tcttacctct atttcttctt tattagttaa tgcttgagat gaatcattgc
     6361 ctaccatgat ccaataagca ccatcatcta taatttcgtt gaactcttca tcataatact
     6421 taaaatcttt gtttgtaagt tcaaaagaaa cagtcacact atcccctgac ttaacaaaaa
     6481 ccttcttgta ggctaccaag tcttttgttg gtcttatata acgaccatat tgagctccaa
     6541 tatatagttg aacaacctca taaccatctc tacttccttt atttgaaacg gtagtagtca
     6601 ccttcaaaca gttcttaccc ttctctttaa tgctaattgg tgacatagaa aaagtggtat
     6661 aactcaaccc atacccaaag ggaaattgtg gttcattgga tacatctaag tatttggtcg
     6721 tattaaactt ttcacttgaa tacggtctac cagtattgaa ataattataa taaataggga
     6781 tttgtcctaa atgtctcgga attgttattg taagctttcc ggctgggttt attttgccac
     6841 aaaggacgtc caaaatagca ttgccagcct ctgctcctag atgccaagcc tcaactaatc
     6901 ccttacatat agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact
     6961 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
     7021 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
     7081 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
     7141 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat
     7201 cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga
     7261 gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg
     7321 agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca
     7381 aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa
     7441 accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata catttcactt
     7501 aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg
     7561 gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga
     7621 ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga
     7681 catctatccc tattttctac acttccactt tactactatc tttgaatcgt acaattttct
     7741 taactcttct catttacctc aattcatttt caagtggaat caactcatca tctaacattt
     7801 tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg tttcgcttct ggatgtttgg
     7861 ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagaa atggttctgt
     7921 tgcaaagttt tccaaaaaat ctattttagt gtaaaattga gaaaaaagac agagaggaca
     7981 gagtaatgaa tcattttaaa ggcaaacaat tcaaaaaaga cgtcattatt gtcgctgttg
     8041 gttactacct gcgttacaat ctaagctatc gtgaagttca ggaattgtta tatgatcgtg
     8101 gaataaatgt ttgtcatact acgatttatc gttgggtgca agagtacagc aaagtcctct
     8161 attatctttg gaagaagaaa aaatagacaa tccttctatt catggaaaat ggacgaaacc
     8221 tatatcaaaa ttaagggacg ttggcattat ctttatcgtg caattgatgc ggacggctta
     8281 accttagata tctggttacg aaagaaacgg gatacgcaag cagcctatgc tttcttaaaa
     8341 cgactccata aacagtttgg tgagccgaaa gcaattgtga ccgataaagc accttctctt
     8401 ggctccgcct ttagaaagtt acagagtgtg ggtttatata ctaagacaga gcaccgaact
     8461 gtgaagtatc ttaacaattt aatagaacaa gaccatcgac ctattaaacg acgaaataaa
     8521 ttttatcaaa gtctccgtac agcctcttcc acgattaagg gcatggagac cattcgagga
     8581 atatataaaa agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgaaatc
     8641 aaggtattaa tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt
     8701 ttcaaacttc gcaacagaac ccttttaagc aatttcccct cttccaaggg aaatacatga
     8761 cggagtcgga ggtgcttgat tacctactct ctatagataa ccaactacga caaagctatg
     8821 aagtctatca agagttatta gctgcttttg atgcaaaaga ttttactgaa tttttcaatc
     8881 tcattgaatc attgccacat tccttgaatg atgggtttaa aaaggcaatt tgttatctga
     8941 aaaagcacac agaagcaatc acaaactcct taaaatatcc ctattcaaac ggaaaattgg
     9001 agggaaagaa taacctcatc aaagtaatca aaagaattgc ttttggattt cggacttttc
     9061 gtcatctccg gatgcgtgtc ctgattcaac agaacatctg tgaaatcatt taataacgca
     9121 aaaaaggaaa tgccgaagca tttcctaaca tacgatttga gcaaaacaat agcaattgac
     9181 aaagaaccta aataatttcc ataaaaaacg cactcctttc caataatttt aggaaaggag
     9241 tgctttattt catataaaat caatttttta tacacatgct cattggctat atttcagggt
     9301 tctcgagaaa taaagcaagc cagtttgaca atcgacataa cgttcttttc caaattgctg
     9361 gcggcttttt ttcctttcca ccattagtat actacagttt ctgcctttca tccttcaggg
     9421 tgaatgagct tgcgaattca tgtatgtttg acaaagaacc tctttttcta aaaatagacc
     9481 gacaaagtac tgcctagaat caacccattt tttaaaataa cgtacgattt ttatttagtg
     9541 gcacgttatg agagtctctc gttcgttttt ctgatctttt tattgaccca actgcctata
     9601 aaacgacgga aaaagtattt catgaataat tcaggtactt ataatcagta caataacctg
     9661 ataaaacccc tagtttaact agggaatccg aagctaagcc ctcttttcca aacccagaga
     9721 gaagaattaa ccctgcgaaa gcaactaatt tcattagttc ttctgaatga ttcaattgac
     9781 ttcacttact cagtatatta attggattca tctcaatcat tttattaaaa gttggttcac
     9841 tcattacttt taatagttct tctttcaaac ttgttaaaat ataggtaagc ccaggcgttc
     9901 ctccataagt tggcaaatat ttttttcgtc ccatatcacc tgaaagacaa atctgatttt
     9961 cataacccgc atcaaccagt cgtttaatta gttggacgcg gtctgcatca tgattcgcca
    10021 actctcggcc aatatgatcc atacaaatac ttgctcccgt ttcacagatt tgagctaaat
    10081 agtgaaaatc tttagttaaa tctacatgac tcaaacaaat attacttgct ttcgcaccgt
    10141 tttttgttaa acaattaatt tgctccagtc ccatcgtccc cccatcacaa tgcgtaataa
    10201 tgggaatccc tgtttttttt tgtacttgac cggctatggc tagtattcgt tgttcagcca
    10261 atgtaacctc atttttactc gtacctactt tcattgctcc tggcaaaagg ttttgtgaag
    10321 tgactccgtg agttatttcg gaataaagca actcaaagat ctcatcatca ttgagcgttg
    10381 tcaaccattt tggctgaaac tcttccttat ggaatcccgt aacaaatgct acattcactc
    10441 cggtagcttc agatatcatt ttcaaagcta acggatttcg tccataatta attggtgtca
    10501 aatcaacgat agtttttcca ccagcttgtt taaaagccat aacttccgca atactatgat
    10561 aaatatcatc caaggtataa tcttcatact ttcttaactc attaggaatg acatataaat
    10621 gttcatgaat aaatgttgta cccaaagact cagcttcgat tgggcctgta accgtattga
    10681 tcatgcactc atctcctacc attcagcgat tgtaccatca taatttttcc acttaggatt
    10741 ggtccaaact aaaccttctt gtgcaatttc tttcactcga tcttcgtcca tttctaaccc
    10801 taaacctggt tttgtcggaa gatctacgaa gccatctttg tattggaata tctctttatt
    10861 ctttacaaaa tctaacaaat caaacccttt gttgtaatga atacctaagc tttgttcttg
    10921 aataaacaca ttaggtgtac aagaatcaac ttgtaatgta gctgctaacg caatcggtcc
    10981 atatggtgca tggggggcaa ctgctatatc ataagcttca gccattgcag caatttttct
    11041 tgtttctaaa atccctccac ataaagctac atctggttga atgatatcaa tcgctccctg
    11101 ttgaagaatt gatttaaatt gccatcttgt atacaaacgt tcacccgttg ctagtggtac
    11161 agcaactgca ttggctactt cactgaaatg ttcttcattc tccggcaaaa ccacttcttc
    11221 taagaacatt ggatgatagg gttccagtgc ttttgctaaa accttagcca ttggtttgtg
    11281 aacacgacca tgaaaatcga caccaatgct caatgtatct ccaaaacgtt ctctaattga
    11341 agcaacacgt tccactactt cagctacttt ttcataagaa tcaatgtagt gtaattcttc
    11401 tgtcgcgttc attttcacgg cggtaaaccc acgatctact cgatcttgtg cctgctctac
    11461 tacttcagaa ggacgatctc caccaatcca agaatatact ttgattttat cacgggctgc
    11521 tccacctagt aattcataaa ctggaacatt taaggctttc cctttaatat cccataaagc
    11581 catttcaagt ccagagacaa tcgttccatt aataggacca cctcggaaaa atgaacgatg
    11641 catttcctga aacagacgtt caattgcaaa tgggttttga ccaactaaga atcgatttcc
    11701 tatctcataa gctcctgcca caactgtttc agtcttagtt ccagaaatca tttcacccca
    11761 gccttcaata ccttcatctg tagatatttt gacaaaaatc catcttggtt ttactttata
    11821 gactgtaatt tttgaaattt tcactgtatg ccctcctgaa tattagaaaa tatttgaaat
    11881 aaactcagca atcttcatta aaatgaaacc aataatgtgt gtacctgcat cttcggtaga
    11941 tatttgtgtc attcctgctg gtaattggaa tcccccagcc aaagctaatt gtgtatgtat
    12001 gccggctaaa ctagtagcaa ttaaaagcac acaagctgtt ataatcgtgg tggaaagtat
    12061 tccgcgaata atatttcctt ttgaaaaggc gaccggccat gcagatagcc acattgcttg
    12121 gtaggcaata tctgaaatag gtaagagttt gttacctggt aaaatagcag ccaacactaa
    12181 ggtaatcgga accatgataa tacccgtagc aatggcactt gaattagcaa ctgtcaacgc
    12241 agcatccaca cccattgtaa aattgtttcc tggaaatttt acattcaaga atttcttaac
    12301 agaattagca aagggtaata atccttccat taaaatttgc atcattttag gtgttagaac
    12361 cattgttgca gaagtactca ttcccaatat caatacctta tcaacaggtt cttttgctaa
    12421 taacccaata accaagccga tgacaaatcc cataaacata ggctcaccaa caaaaccaaa
    12481 ccgctcttga attttttcag gacgagcatc taccttattt aatccaggaa tgcggtccca
    12541 caatttgtta agtagccaag ccaagggtgc ccaaccaact gagttcgcag tcgggaaatt
    12601 aaccccttct agttcaaagt aatcctctac taacggcgtt gtccaatcag cgagtttaaa
    12661 actaataatt gcatcaatcg ccccgaagat taccgccaat ataatatttt tatccgttgc
    12721 ataataaacc aaagcagcta caaaaatata gtgattataa ctccagaaat cgaccattac
    12781 tgtattggtc tttttaaaaa tcagcatcaa cacattaata cccaaaataa taaataccat
    12841 aactgctgct agaggaaaag accaagtggc tgctgcacga gctggccagc cgacatccat
    12901 aatacttgtt tgaatacccc aatttttgac cattaactga acagatggac ctacctgtga
    12961 aataaaccaa ttaataataa tatttacacc cgcaaaacca atccccacag tcaacgctga
    13021 ttttagtaat gtatttagct taattctaaa aaataatcct aaaatcaaga taatgatagg
    13081 catcataatc atcggaccca aacttgagat ccagtttaac gcatcaatga caatattcat
    13141 ttttcaccct cctagtcaag ctctttcaat gcttctaaca ccaaagattc agatttttct
    13201 tcattcactc cagtaacaaa tggtgttcca ggtataactt taattccttt ggttacattt
    13261 tcttgtaatt tccctgtagg aacgataaag tcaacttgat ttgattctaa agcacctgca
    13321 acttctgaaa attttagttg caaaattcga ggttctggta atccatttgt ttttgctatt
    13381 ttccgaattt tattagctgc cattgtagat gtaatcattg atgatgcaca tgcgacgata
    13441 attgtttttt tactcattgt atttccctcc aatttattga ttaaatgcag ttaaaacttc
    13501 tgttttactc tttgcttcca taattgcttg taagaaatcc tgattggtaa ctttactcat
    13561 caactccttc aataattgcg gttgcttatc gggactatcg aataaaaggt gaataataac
    13621 attcactgaa accatttctt cgggatcatc cattcgctga aaatgcaaac tatctttaag
    13681 aacggatact aaaatagcgg taa
//
