LOCUS       NZ_CP044260            10060 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP65 plasmid p3, complete sequence.
ACCESSION   NZ_CP044260
VERSION     NZ_CP044260.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12813532
            Assembly: GCF_008728415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   He,F.
  TITLE     Genome sequence of Klebsiella pneumoniae KP65
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2019) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 01:36:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 41 of 144
            Pseudo Genes (incomplete)         :: 101 of 144
            Pseudo Genes (internal stop)      :: 17 of 144
            Pseudo Genes (multiple problems)  :: 15 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP65"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2018-05-18"
                     /collected_by="Fang He"
     gene            54..332
                     /locus_tag="F6W81_RS28285"
                     /old_locus_tag="F6W81_28295"
     CDS             54..332
                     /locus_tag="F6W81_RS28285"
                     /old_locus_tag="F6W81_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            320..634
                     /locus_tag="F6W81_RS28290"
                     /old_locus_tag="F6W81_28300"
     CDS             320..634
                     /locus_tag="F6W81_RS28290"
                     /old_locus_tag="F6W81_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            798..1226
                     /locus_tag="F6W81_RS28295"
                     /old_locus_tag="F6W81_28305"
     CDS             798..1226
                     /locus_tag="F6W81_RS28295"
                     /old_locus_tag="F6W81_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1252..1431
                     /locus_tag="F6W81_RS28300"
                     /old_locus_tag="F6W81_28310"
     CDS             1252..1431
                     /locus_tag="F6W81_RS28300"
                     /old_locus_tag="F6W81_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1458..1988)
                     /locus_tag="F6W81_RS28305"
                     /old_locus_tag="F6W81_28315"
     CDS             complement(1458..1988)
                     /locus_tag="F6W81_RS28305"
                     /old_locus_tag="F6W81_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1995..2726)
                     /gene="mobC"
                     /locus_tag="F6W81_RS28310"
                     /old_locus_tag="F6W81_28320"
     CDS             complement(1995..2726)
                     /gene="mobC"
                     /locus_tag="F6W81_RS28310"
                     /old_locus_tag="F6W81_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2726..4690)
                     /locus_tag="F6W81_RS28315"
                     /old_locus_tag="F6W81_28325"
     CDS             complement(2726..4690)
                     /locus_tag="F6W81_RS28315"
                     /old_locus_tag="F6W81_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6174..6323)
                     /locus_tag="F6W81_RS28325"
                     /old_locus_tag="F6W81_28335"
     CDS             complement(6174..6323)
                     /locus_tag="F6W81_RS28325"
                     /old_locus_tag="F6W81_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6408..6665)
                     /locus_tag="F6W81_RS28330"
                     /old_locus_tag="F6W81_28340"
     CDS             complement(6408..6665)
                     /locus_tag="F6W81_RS28330"
                     /old_locus_tag="F6W81_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6675..8360)
                     /locus_tag="F6W81_RS28335"
                     /old_locus_tag="F6W81_28345"
     CDS             complement(6675..8360)
                     /locus_tag="F6W81_RS28335"
                     /old_locus_tag="F6W81_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8687..8932
                     /locus_tag="F6W81_RS28340"
                     /old_locus_tag="F6W81_28350"
     CDS             8687..8932
                     /locus_tag="F6W81_RS28340"
                     /old_locus_tag="F6W81_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9206..9577
                     /gene="tolA"
                     /locus_tag="F6W81_RS28345"
                     /old_locus_tag="F6W81_28355"
     CDS             9206..9577
                     /gene="tolA"
                     /locus_tag="F6W81_RS28345"
                     /old_locus_tag="F6W81_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            9574..9939
                     /locus_tag="F6W81_RS29270"
     CDS             9574..9939
                     /locus_tag="F6W81_RS29270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1675..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5493..6071
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1999..2726)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cgccacggat taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat
       61 cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg
      121 accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg
      181 aagagaagcg cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa
      241 cgggtctgca ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga
      301 acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt
      361 acaacgcctt caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga
      421 ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc
      481 gtttctgccg ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat
      541 gctctaccgc cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa
      601 agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat
      661 tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa
      721 aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat
      781 aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg
      841 cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac
      901 gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg
      961 tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt
     1021 tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca
     1081 ccctgatgct ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc
     1141 gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac
     1201 gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag
     1261 caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg atcagtcgct gctgttgctg
     1321 gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag
     1381 gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg
     1441 ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc
     1501 tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc
     1561 gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc
     1621 gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg
     1681 gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc
     1741 cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc
     1801 cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa
     1861 cagaaaacat acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg
     1921 gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac
     1981 agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg
     2041 tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc
     2101 agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca
     2161 tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg
     2221 cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc
     2281 gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc
     2341 ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc
     2401 gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac
     2461 gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg
     2521 tatttcgccc ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc
     2581 tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc
     2641 cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg
     2701 ctgacgttgt actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg
     2761 gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg
     2821 ggaaggtttt cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc
     2881 gcgccacgcc cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca
     2941 cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt
     3001 tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc
     3061 ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt
     3121 tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt
     3181 cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg
     3241 cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg
     3301 gccacctccg gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct
     3361 tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc
     3421 cgttgattat ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca
     3481 cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt
     3541 acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct
     3601 tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt
     3661 taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca
     3721 gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga
     3781 agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat
     3841 cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc
     3901 cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct
     3961 gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg
     4021 ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc
     4081 cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag
     4141 cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg
     4201 cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat
     4261 aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca
     4321 cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt
     4381 agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca
     4441 ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg
     4501 ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa
     4561 acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga
     4621 cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg
     4681 tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc
     4741 cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat
     4801 tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc
     4861 cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg
     4921 tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca
     4981 aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt
     5041 ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg
     5101 acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag
     5161 gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga
     5221 gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac
     5281 ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca
     5341 tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg
     5401 gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca
     5461 cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt
     5521 ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg
     5581 tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca
     5641 ggacttaaag atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga
     5701 ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct
     5761 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     5821 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     5881 ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc
     5941 agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac
     6001 actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt
     6061 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg
     6121 gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
     6181 accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc
     6241 cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga
     6301 aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc
     6361 ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg
     6421 tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa
     6481 aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta
     6541 tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa
     6601 tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc
     6661 cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc
     6721 tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct
     6781 tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc gccataccat
     6841 cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct
     6901 ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta
     6961 gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt
     7021 ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag
     7081 gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga
     7141 atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg
     7201 ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc
     7261 tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag
     7321 gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc
     7381 agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga
     7441 cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt
     7501 ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca
     7561 gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga
     7621 acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc
     7681 cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca
     7741 accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc
     7801 gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat
     7861 gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca
     7921 ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca
     7981 tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc
     8041 ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca
     8101 gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta
     8161 ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag
     8221 cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta
     8281 ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct
     8341 cgaccgtctc caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc
     8401 caacatactg tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc
     8461 aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc
     8521 agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca
     8581 tcacacttac atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga
     8641 cctgcggtta aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa
     8701 actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat
     8761 gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt
     8821 gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa
     8881 atacgaggct acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt
     8941 taccataacg tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac
     9001 gggataaaac aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta
     9061 acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg
     9121 ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat
     9181 tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg
     9241 cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac
     9301 tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat
     9361 gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca
     9421 acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc
     9481 agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca
     9541 aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg
     9601 gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag
     9661 ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt
     9721 gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt
     9781 cgggaaacgc tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga
     9841 caggttataa attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc
     9901 accacccaaa agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc
     9961 tgcaatagtc ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg
    10021 aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc
//
