LOCUS       NZ_CP044153            27094 bp    DNA     circular CON 09-JUL-2023
DEFINITION  Shigella flexneri strain AR-0425 plasmid pAR-0425-1, complete
            sequence.
ACCESSION   NZ_CP044153
VERSION     NZ_CP044153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12648975
            Assembly: GCF_008727235.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27094)
  AUTHORS   Chen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2019) EDLB, Centers for Disease Control and
            Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164.45x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/09/2023 02:56:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,901
            CDSs (total)                      :: 4,773
            Genes (coding)                    :: 3,978
            CDSs (with protein)               :: 3,978
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 795
            CDSs (without protein)            :: 795
            Pseudo Genes (ambiguous residues) :: 0 of 795
            Pseudo Genes (frameshifted)       :: 329 of 795
            Pseudo Genes (incomplete)         :: 417 of 795
            Pseudo Genes (internal stop)      :: 170 of 795
            Pseudo Genes (multiple problems)  :: 121 of 795
            Pseudo Genes (short protein)      :: 5 of 795
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="AR-0425"
                     /db_xref="taxon:623"
                     /plasmid="pAR-0425-1"
     gene            join(26928..27094,1..115)
                     /locus_tag="F4V37_RS23265"
     CDS             join(26928..27094,1..115)
                     /locus_tag="F4V37_RS23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            261..584
                     /locus_tag="F4V37_RS23270"
     CDS             261..584
                     /locus_tag="F4V37_RS23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001181903.1"
                     /translation="MQNYAKSVATEILRQLGGNRFIVMTGAKSFSYFDENGECGLTFRL
                     PSNFAMKGINLVKIKLDFTDTYQVKFSRVRGAEVKDISRFDNIYCDQLARLFTQETGLH
                     TVL"
                     /name="hypothetical protein"
     gene            629..874
                     /locus_tag="F4V37_RS23275"
     CDS             629..874
                     /locus_tag="F4V37_RS23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000356546.1"
                     /translation="MDKDKIIKKNRGNYSYVIRTMDEDGDTVFHVLKYVKTIDKTKSRK
                     TVRKLIMDEKLNLASLMLLDNGVLCDCLTKGDENAE"
                     /name="hypothetical protein"
     gene            864..1106
                     /locus_tag="F4V37_RS23280"
     CDS             864..1106
                     /locus_tag="F4V37_RS23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_075330262.1"
                     /translation="MQNKPTPEEVKNARVAAGLTLKEAADIFGYQLNSWQMKESAGKAS
                     RSLSIGEYQYLLLLANMHPSYRLVKNNLPYLQMQV"
                     /name="hypothetical protein"
     gene            1171..1500
                     /locus_tag="F4V37_RS23285"
     CDS             1171..1500
                     /locus_tag="F4V37_RS23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000866648.1"
                     /translation="MKVLTFKNDTVSVGDIFVSSWGYEQTNVTFYQVLSVHGKKTVTVR
                     EIRANSEYTDSMVGFKTPVLNDFTGECFKRQIKDFGDELAIKIEDFETAYKTLPEEKHR
                     FSSYY"
                     /name="hypothetical protein"
     gene            1786..1935
                     /locus_tag="F4V37_RS25175"
     CDS             1786..1935
                     /locus_tag="F4V37_RS25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000003880.1"
                     /translation="MSERILSAINDVEKGGRPVFPLMPFHVFPEYMALLRKALEKKTQK
                     RTDK"
                     /name="hypothetical protein"
     gene            1948..2220
                     /locus_tag="F4V37_RS23290"
     CDS             1948..2220
                     /locus_tag="F4V37_RS23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160399.1"
                     /translation="MTKHDFVSFVSGELRQGAVRFSLAFNSKGEIVLHWTNKAGIRVWR
                     ILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
                     /name="hypothetical protein"
     gene            2546..3091
                     /gene="ddp1"
                     /locus_tag="F4V37_RS23295"
     CDS             2546..3091
                     /gene="ddp1"
                     /locus_tag="F4V37_RS23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_015060502.1"
                     /translation="MKKVQFRIDENQHDDLLDCLKTLYPDEPALTVAKGMKLLANALLK
                     SKAVSKDINTFFDNNDFIKTTMYLTGKQRADIERAANRHGWTLSRECRYRIQTTLENEL
                     DFFDQELLMMNRCRNSIDKIGRNFHHIIVNDQTRVLDKDGFYQDAERLTTEIFNLKNQF
                     ENYIMLCKGRTVSNKVEM"
                     /name="ddp1"
     gene            3094..4259
                     /gene="mobP1"
                     /locus_tag="F4V37_RS23300"
                     /pseudo=""
     CDS             3094..4259
                     /gene="mobP1"
                     /locus_tag="F4V37_RS23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobP1 family relaxase"
                     /name="mobP1"
     gene            4605..5264
                     /locus_tag="F4V37_RS23305"
     CDS             4605..5264
                     /locus_tag="F4V37_RS23305"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_252997309.1"
                     /translation="MKGQKQFYLLQCSPHFHQSVLLQLSRSGIAYYNPVIRTFYKRRDC
                     SAYRGQIMPMFPGYVFVLLDFEVVHPSCFTRMKNVYGLVSFGEYPAEVPLSVINEVKEQ
                     EKIFSMNLSLMNNSCLAKILLLADASKRGQVFADYVCNRKYDRLKMTAIKGKRKPQRNV
                     LYLPTEVRVEVEKIAIEISFKRGRRISDSGFVQYLIKKYKSQAMNELIHGADIPDE"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            5297..5944
                     /locus_tag="F4V37_RS23315"
     CDS             5297..5944
                     /locus_tag="F4V37_RS23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000953529.1"
                     /translation="MLSTSTFLALAMQCAASVHPDTTHEVARVESGFNPYAIAEIIPKV
                     KRKPGDKGVVSYFPESKEAALKIVKNIELRNHRYSVGLMQITSTNFAKFGTTAEKMFDP
                     CENLKVSEKILVDCYKRGGDLVRGLSCYYSGNPETGVKPEPEFNNTSYVQRIGFSPPDN
                     KKSFIVPSVKEMIKKENKTTITPEEIIIYPQYAMRGTVSNEKETKDVEIKSE"
                     /name="lytic transglycosylase domain-containing protein"
     gene            5922..6218
                     /locus_tag="F4V37_RS23320"
     CDS             5922..6218
                     /locus_tag="F4V37_RS23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000916182.1"
                     /translation="MLKLNLNKRYLTLSLFMAALMLCVAEPAFADDVSTKTTGFLQKII
                     DFLTDIRKPAITIIALVIGYIAIFSRQHTSWIVPLVIGIIIFIVAPYIPDWLA"
                     /name="TrbC/VirB2 family protein"
     gene            6237..7178
                     /locus_tag="F4V37_RS23325"
     CDS             6237..7178
                     /locus_tag="F4V37_RS23325"
                     /inference="COORDINATES: protein motif:HMM:NF016957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_252997310.1"
                     /translation="MSTLFKGLTRPALIRGLGVPLYPFLGMCIICVLLGVWIHEAMYAL
                     ILPGWYAIRRVTQFDERFFDLLYLRTLVKGHPLSNKRFSAVHYAGSQYDEVDISKVDNF
                     MKLKDQSSVEELIPYSSHITDNIIVTKNRDLLATWQIDGAYFECVDSEDLSILTDQLNT
                     LIRSFEGKSVTLYPHRIRCKKDVRPVFNSKIPFVNRVMNDYYESFPQSEFFENKLFLTI
                     CFKPFTTEDKVTHFFSRSKKQKDIFKEPVNEMNEICDRLNTYLSRFHSRRLGLYEDHGV
                     VYSDQLSLFQYLLSGRWQKVRVSSSPFYGFVE"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(7220..8186)
                     /locus_tag="F4V37_RS23330"
                     /pseudo=""
     CDS             complement(7220..8186)
                     /locus_tag="F4V37_RS23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(8188..8481)
                     /locus_tag="F4V37_RS23335"
     CDS             complement(8188..8481)
                     /locus_tag="F4V37_RS23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015060510.1"
                     /translation="MDKNVVDALKTLLEALPEEVVTEVTSKLNPSASHIPEENSKQLTA
                     KARLLNFRLTEAYEEILEVEAIRTGQSKTTVLKAALAMYNSQEALLNKSNFC"
                     /name="hypothetical protein"
     gene            complement(8497..8726)
                     /locus_tag="F4V37_RS23340"
                     /pseudo=""
     CDS             complement(8497..8726)
                     /locus_tag="F4V37_RS23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /name="plasmid partition protein ParG"
     gene            complement(8778..9398)
                     /locus_tag="F4V37_RS23345"
     CDS             complement(8778..9398)
                     /locus_tag="F4V37_RS23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864791.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000864791.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKSFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVSTFE"
                     /name="ParA family protein"
     gene            complement(<9588..10757)
                     /locus_tag="F4V37_RS23350"
                     /pseudo=""
     CDS             complement(<9588..10757)
                     /locus_tag="F4V37_RS23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /name="DNA topoisomerase"
     gene            10892..11275
                     /locus_tag="F4V37_RS25735"
     CDS             10892..11275
                     /locus_tag="F4V37_RS25735"
                     /inference="COORDINATES: protein motif:HMM:NF033859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="WP_252997311.1"
                     /translation="MLHKKSAQENVAFGLAVLKYYLRFNSKENLQDANVSSEELISNLI
                     NILKDTNFVCTSEISATYPGIDALDKENKLGLQITHTKNTSKINKTIDKIRINKVNEEI
                     DTLYFSLHRKSRKNTLSKHNVQN"
                     /name="SMEK domain-containing protein"
     gene            11368..11814
                     /locus_tag="F4V37_RS25740"
     CDS             11368..11814
                     /locus_tag="F4V37_RS25740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231436499.1"
                     /translation="MKAMPRLYQEGKERYQAMLNNILETRNKLDRRVFTANKILEEPEE
                     MLLSLKEIRIDIQKDGIMNKANPAVSDSFNKIINIIDDVESKIAVQYPDEYKKYKAKIL
                     PSWNSPEKEECLDILMAIRKDVLEQIDNIDIEVNKLKSILDNNI"
                     /name="hypothetical protein"
     gene            complement(11928..12230)
                     /locus_tag="F4V37_RS23360"
     CDS             complement(11928..12230)
                     /locus_tag="F4V37_RS23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000717624.1"
                     /translation="MKKICKSLIVFFTVSWLTAPAYASDPCASVLCLYGKAVGQGGGSE
                     CRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(12227..12639)
                     /locus_tag="F4V37_RS23365"
                     /pseudo=""
     CDS             complement(12227..12639)
                     /locus_tag="F4V37_RS23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(12636..14468)
                     /locus_tag="F4V37_RS23370"
     CDS             complement(12636..14468)
                     /locus_tag="F4V37_RS23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_151090839.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNF
                     DSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFAS
                     DNDLRKSKLLKWEKENDTGILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLV
                     RKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKD
                     LLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIG
                     SVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIRESRTEDEQRSSIDGSF
                     RKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEV
                     TLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEI
                     YGIEGAKTLMSAHPCRIIYAVSEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESES
                     EARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTM
                     ELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(14471..15501)
                     /gene="virB11"
                     /locus_tag="F4V37_RS23375"
                     /pseudo=""
     CDS             complement(14471..15501)
                     /gene="virB11"
                     /locus_tag="F4V37_RS23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058463.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /name="virB11"
     gene            complement(15503..16708)
                     /gene="virB10"
                     /locus_tag="F4V37_RS23380"
     CDS             complement(15503..16708)
                     /gene="virB10"
                     /locus_tag="F4V37_RS23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000139139.1"
                     /translation="MTEQENKIPTATEIEQQLRERRQKELEQAGKTPEEEPGKPALQLG
                     IEKLKKSRKGMIILVVGFLLLAAGVSVYYIPSIIRSVSSGDEKPASQPLATGAAKRQTG
                     LSEDIDPFNTAQKKTEKPEEEKVISSEKTEPPENKQQSFSRALDVSLDGSQAGNSSSSA
                     GTSVSHTAASEPESDKKDEAKATAQATESAPLAKITKLPYDPNLFIPEGTSIPCSLDRR
                     FVSDLAGKLECTVNSDIYSASGNVKLIERGTAAKLMYKAGSLNHGQGRVFVMAYKLRTR
                     SKPFIDIPLVDSQAAGALGEAGASGWIDTHFSERFLGAMMVGMIPDLSQAASGIAQNNR
                     DSQTDYTANSRQAFADIAREAFSNSVNIPPTLYKNQGEIITLIVGQDLDFSGIYKLKMK
                     GG"
                     /name="virB10"
     gene            complement(16705..17634)
                     /locus_tag="F4V37_RS23385"
     CDS             complement(16705..17634)
                     /locus_tag="F4V37_RS23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="WP_000783379.1"
                     /translation="MKMNKGALIMALLMAAHVCNAAVLPSGSRFDPRNQIVSYNPNNTT
                     IINSAVGYTTTLVFDEDETVISARTGFPQGWAVNKEDNLVYLEVRPVKQTVQKNNTDAN
                     GNTSSESVSVALDPENELERWRTNLFVRTTKRNYSMELNARTFRQPEKIAFVVNYQYPQ
                     ERRKEQAEIEKKRTEALARRLEEQAINRSLENAKSPRNWQYWKRVAEGSQDISPDYAYD
                     DGRYTWFGFSPLKKIPSVFVMNGMQETLTNPVIKQSGSFTAVGVPVDKRFVLRLGEQVV
                     GIENLGFGKVRLPAGDTVSPDVKKEVIQ"
                     /name="TrbG/VirB9 family P-type conjugative transfer
                     protein"
     gene            complement(17639..18352)
                     /locus_tag="F4V37_RS23390"
     CDS             complement(17639..18352)
                     /locus_tag="F4V37_RS23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000394613.1"
                     /translation="MEFKLPGFKNKKDVTDSSVSFEEKNIALQERMNRIYKFGGIGGML
                     IGGLSLLALNAALPLKTTVVDAYLIDKVTGVAERLTSVKKENLSENEAIARYFITQYIK
                     HREGYNFFSLQHDYDYVMAYSAENVAADYNALFNSEQAPKLVYNKAEKTAMVQDNPSVI
                     ISPSSRTDDKDIGAYIRFRLTIRDVATGQTRQEFWNVRLTYRIEPQVEMVSGERNNNPL
                     KFVVTSYVRDKEARG"
                     /name="type IV secretion system protein"
     gene            complement(18342..18470)
                     /locus_tag="F4V37_RS25745"
     CDS             complement(18342..18470)
                     /locus_tag="F4V37_RS25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012769216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001328099.1"
                     /translation="MKLLIVAFVTLCLAGCQASHKLPPVSGKSEPVNSAEVMQNGI"
                     /name="hypothetical protein"
     gene            complement(18597..19658)
                     /locus_tag="F4V37_RS23395"
     CDS             complement(18597..19658)
                     /locus_tag="F4V37_RS23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000796673.1"
                     /translation="MAEASNFFVTAKEEILSVLDASVSGQLTSLSNIAGTLGRYGVSIY
                     VLWYAFTVLARKQQTPVPDFIWNLCRFSIILMFVQNAGGWLNTSLVAIDGLKDSLSGNN
                     DAWLWLDQLWTKVQQVAAHVMSKDTSTYVKTDGAIAALFTYFGGILALVACAIVFLAAE
                     VTLKLLTVTAPVFIFCLMFGFLRQMFNSWLQLIFSSCLIFMFGSLALRAGTSFLNIVLS
                     LAIDTVDNRNLIKIGATAAVAGIFMAWIIWQAKSYASQIAGVGVEGAMQGAAAMGIGAG
                     VFGASRMARGALGMGRNAGIGAWKGLRRQEGGFGQSPGITGKTANLAGQGVNIGAKKLR
                     QAAIERAKKMYGG"
                     /name="type IV secretion system protein"
     gene            complement(19651..19935)
                     /locus_tag="F4V37_RS23400"
     CDS             complement(19651..19935)
                     /locus_tag="F4V37_RS23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_151090840.1"
                     /translation="MIGALSIFSLVGCEETKSVDWWLEHHEESLKKEAECKKTGSDSQN
                     CRNVKEANFRYQQLHAKEPNFKEYFKKYKDASNHEEKVMCLKNKETRDG"
                     /name="EexN family lipoprotein"
     gene            complement(19967..20716)
                     /locus_tag="F4V37_RS23405"
     CDS             complement(19967..20716)
                     /locus_tag="F4V37_RS23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000744202.1"
                     /translation="MKKQIMAAFVASLIVISGAQAGIPVAIDANPEWAIEAGRWTERLK
                     QWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDYIK
                     NPEGALSEQAKSLLSDYKVTDTCQNLGYSGDLVRGCEATFLSQLASVEYGNKLESKLRQ
                     DNQTMKGLIDQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYRDKQRDLAEYKEKM
                     AQAAFRKQQLEAEEPSFKDAARRYTLN"
                     /name="type IV secretion system protein"
     gene            complement(20727..21212)
                     /locus_tag="F4V37_RS25750"
     CDS             complement(20727..21212)
                     /locus_tag="F4V37_RS25750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024196033.1"
                     /translation="MDEFWQWINNEAFRDFVYNKLKTGRKLDMVLVVATQSPDELIKSP
                     IAAAVREQCATHIYLANPKAKRSEYVDGLQVRELYFDKIKAIDPLSRQFLVVKNPQRKG
                     ESDDFAAFARLELGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLEQANLI"
                     /name="hypothetical protein"
     gene            complement(21149..22504)
                     /locus_tag="F4V37_RS23410"
     CDS             complement(21149..22504)
                     /locus_tag="F4V37_RS23410"
                     /inference="COORDINATES: protein motif:HMM:NF015118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252997312.1"
                     /translation="MTASDHARYFRCIEIKDYFQETDAGILDALMYLPVEYVVTSSFTA
                     MDKQSAIKALDDQIDKLEMTDDAAKSLLADLKVGLDMVSSGYISFGKSHQTLVVFADSP
                     ERLVKDTNIVTSTLEDLGLIVTYSTLSLGAAYFAQLPGNYTLRPRLSTLSSLNFAEMES
                     FHNFFSGKEKGNTWGEKLITLRGSGNDIYHLNYHMTTEHQNFFGKNPTLGHTEILGTSN
                     VGKTVLLMTKAFAAQQFGTPESFPANRKLKKLTTVFFDKDRAGEVGIRAMGGSYYRVKE
                     GEPTGWNPAALPPTKRNIAFMKDLVRLLCTLNSEPLDDYQNSLISDAVERLMQRSDRSY
                     PISKLRPLIQEPDDTETKRHGLKARLKPWTQGKSLAGCSTIGKTRLMSITWMFSVLMER
                     NSWIIRCWPVLLHSISSIGSLCWPMVAGFLSTWMSSGNGSITKRSGTLFTTS"
                     /name="hypothetical protein"
     gene            22594..23562
                     /locus_tag="F4V37_RS23415"
     CDS             22594..23562
                     /locus_tag="F4V37_RS23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_077783213.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWSGEYADRNRAVANQRMTGSDARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(23622..23975)
                     /gene="ddp1"
                     /locus_tag="F4V37_RS23420"
     CDS             complement(23622..23975)
                     /gene="ddp1"
                     /locus_tag="F4V37_RS23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000876434.1"
                     /translation="MKYLVKEFINEKYTKAVNILKDNLKEHYHVFYCVRLSEILFPASE
                     YGTEQFFSDFEKINSISLPVLVFDLKEGVPVIVISLDDVSYCDVLDEMDIDFMKCDSLA
                     ELLTSDKLENLLV"
                     /name="ddp1"
     gene            complement(24112..24557)
                     /locus_tag="F4V37_RS23425"
                     /pseudo=""
     CDS             complement(24112..24557)
                     /locus_tag="F4V37_RS23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(24740..25432)
                     /locus_tag="F4V37_RS23430"
     CDS             complement(24740..25432)
                     /locus_tag="F4V37_RS23430"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_252997308.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKILKK"
                     /name="replication initiation protein"
     gene            26687..26938
                     /locus_tag="F4V37_RS23445"
     CDS             26687..26938
                     /locus_tag="F4V37_RS23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     Typing          complement(24113..24557)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3095..3456
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.347"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3454..4256
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.881"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(12676..14468)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.831"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(16709..17634)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.382"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17643..18352)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.578"
                     /coverage="100.0"
                     /evalue="4.82e-137"
     Typing          complement(18601..19658)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.717"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(21378..22564)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.737"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcatctggc tacagacttg tataccaagt cattgatgag aaagtcgttt tactcgttat
       61 tgccgttgga agaagggaaa gcagcgaagt ctatcagatc gcagatttgc gctaagatta
      121 atcatcaaat gattgactgt ttacagtcag agcgagagca ttaaaaattt gcaatacctg
      181 caaatgcagg tataataaaa cacaagaaag ggaaacaggt tctctttcat ccagagccca
      241 aagtgggcgg gaggttaaaa atgcaaaact acgctaaatc tgtagccaca gagattttac
      301 gtcaacttgg tggtaatcgt tttattgtta tgactggtgc taaaagtttt tcttactttg
      361 atgaaaacgg tgagtgtggg ttaactttcc gtttgccgtc aaattttgca atgaaaggca
      421 tcaacttagt aaaaattaaa ctggatttta ctgatacgta ccaggtgaaa ttttctcgtg
      481 tacgtggtgc tgaagttaaa gatatttcaa gattcgataa tatctattgt gatcagttag
      541 cgcgtttatt tacacaggaa acagggttac ataccgtgtt atagataaaa aggggcatta
      601 agcccctttt ttagttatga ggaataatat ggataaagat aagataatta agaaaaacag
      661 aggtaattac tcctatgtaa tcagaacgat ggatgaagat ggggatacgg tttttcacgt
      721 cttaaaatat gttaagacga ttgataaaac taaaagcagg aaaacagtaa gaaaattgat
      781 aatggacgaa aaactcaacc tggcatcatt gatgcttctg gataatgggg ttttgtgtga
      841 ttgtctgaca aaaggggatg aaaatgcaga ataaacctac acctgaagaa gtaaagaatg
      901 cgcgggttgc ggcaggtctt actcttaaag aagctgctga tatttttggt tatcaactga
      961 attcctggca gatgaaagaa agtgcaggta aggccagtcg ttctttatct attggtgaat
     1021 atcagtattt attgttatta gcaaatatgc atccgtctta caggctggta aaaaataact
     1081 tgccatacct gcaaatgcag gtataataaa acacaggaaa gggaaacagg ttctctttca
     1141 tccagagccc aagctgggcg ggagctaaaa atgaaagtat taacttttaa aaatgatact
     1201 gtctctgttg gtgatatctt tgtatcatca tggggctatg agcaaaccaa tgtaaccttc
     1261 taccaggttc tttctgttca cggtaaaaaa accgtcaccg ttcgcgagat tcgcgccaat
     1321 tcagaatata ccgattcaat ggtcggcttt aaaactcccg ttttaaatga ttttactggt
     1381 gaatgtttta agcgtcagat aaaagatttt ggtgatgagc tggcaatcaa aatcgaagat
     1441 tttgaaactg cgtataaaac tctaccggaa gaaaaacatc gattttcttc ttactactaa
     1501 ttaatcaggg ggatattccc ccttttagta aaggtctgaa aatggaaaga gagtttagcg
     1561 caaaagcatc attaaaccga aaaattttgg tttgagcaat gtggattatc taaagaaaga
     1621 gttattcatt gtattgataa ctggtatgac cttgcatacc caccatcaga acaggagaaa
     1681 gcaaaaaaag aagcaattga gaagttaata aagtaaagat aaagatggta tcaggttgtg
     1741 gggcaacctg ataccgtgac acctgattct attgcgagga ataaaatgtc ggaacgcata
     1801 ttatcagcaa taaatgacgt tgaaaagggt gggcgtccgg ttttcccttt gatgccattc
     1861 catgtctttc ctgagtatat ggcattactc agaaaagcac tggaaaaaaa gacacaaaag
     1921 agaacagata aataaaccgg aggctttatg acaaaacatg attttgtgtc ctttgtcagt
     1981 ggagaattgc ggcaaggcgc tgtacgcttt tctcttgctt ttaacagcaa aggagaaatt
     2041 gttctgcact ggactaataa agcaggaata cgagtctggc ggatattaag tggtaatcgt
     2101 ggcaaaaaac caagcaaagc taaccttgaa agaatgagta acttccggcg ctggcttttt
     2161 gatgcccgtc agggcatgga aggctacact cagcaacctg aacagagcaa ccttagctga
     2221 atagccgaag ccctcccgta tttcgggagg tgctttctga tatattgcgc gggactgcgc
     2281 ttgcgcttcc tgccagaccg ttcgcggagc gaacctgacg gcactgtcct taccgcattg
     2341 atcgggtttc cggtcgcaga cgtacgtatg ttcagccatg acaacatcag cctttttgca
     2401 tttttgttga tgctcaagca caatttatga gcggcgccag attcactgcg atgcaggata
     2461 gggcaaacgc cgcaaaatga cgtctctgac gccattccgc aacattttcc cggtgaccta
     2521 cagtcggttc ttgttggaga attttatgaa aaaagttcaa ttcagaattg atgaaaatca
     2581 gcatgatgat ttgctggatt gccttaaaac tctttatccg gatgaaccag ctttaacagt
     2641 agctaaaggc atgaaacttt tagcaaatgc tttattaaaa agtaaagctg tcagtaagga
     2701 tataaatacg ttttttgata ataatgattt tatcaaaaca acgatgtact taacaggtaa
     2761 acaaagggct gatattgaaa gagctgctaa tcgtcacgga tggacgttat cacgagaatg
     2821 tcgttaccgc atacagacga cacttgaaaa tgaactggat ttctttgacc aggaactgct
     2881 gatgatgaat cgttgccgta attcaattga taagatcggt cgtaatttcc atcatatcat
     2941 tgttaatgat cagaccaggg ttcttgataa agatggtttc tatcaggatg cggagcgtct
     3001 tacaacagaa atttttaatc ttaagaatca gtttgagaat tacattatgt tatgtaaagg
     3061 gagaactgtt tcaaataaag tggagatgtg attatgggcg tttacgttga taaagaacat
     3121 cgtgttaaac gaaagtcatc agaaaatggt cgtaagtcag ctttcgctca caaagtcaaa
     3181 aatggtggaa agaactatag ccgcaatgtt caggaacgta tcaaccgcaa gggtgccagt
     3241 aaggaggttg ttgtcaaaat atctggaggt gctgttactc gtcaggggat tcggaacagt
     3301 attgattata tgagccgtga gtcagagcta ccagtgatga gtgaaagcgg tcgggtatgg
     3361 acgggtgacg aaattctgga agctaaagag cacatgatag atcgtgctaa tgatcctcag
     3421 catgtgatga atgataaagg taaagaaaat aaaaaatcac acagaatatt gtcttctcgc
     3481 ctccagtttc agcgaaagta aagcctgaag atttgctgga gtctgtcagg aaaacgatgc
     3541 agaaaaaata tcctaatcac cgttttgttc ttggatacca ctgtgataag aaagaacatc
     3601 ctcacgttca tgttgttttt cgtatccgag ataatgacgg taaacgcgct gatatcagga
     3661 aaaaagattt acgggaaatt cgtacaggtt tttgtgaaga gttgaagtta aaaggttatg
     3721 acgttaaagc gacccataag caacagcatg gacttaatca gtctgttaaa gatgcacata
     3781 atacagcacc aaaaagacag aaaggtgttt atgaggttgt tgatattggc tatgaccatt
     3841 atcagaacga taaaacaaag tctaagcaac attttataaa gctaaagact cttaacaagg
     3901 gggttgagaa aacatactgg ggggctgatt ttggggactt atgttcgcgg gaaagcgtta
     3961 aagcaggtga tcttgtcaga ctgaagaaac ttggtcagaa agaagtaaaa atcccggcgc
     4021 tcgataaaaa tggtgttcag catggctgga aaacggttca cagaaatgag tggcagttag
     4081 aaaatctggg ggttaagggc gtagacagaa caccttcagc cagcaaagag ctggtactga
     4141 acagccctga tatgctgctg aagcaacaac agcgaatggc gcagtttacg cagcagaaag
     4201 catccacgtt acagtcagaa cagaagctga aaacagggat taagtttttt aatatttaat
     4261 gtattgatta tgcgagaaaa atatttattt tagttcgctt gctcatcttt tcgccttaaa
     4321 aaaagatcga aattctgcgt tttagctatc attcgatcta tcgatctaaa aaacagatct
     4381 tcaaaagatg aatatactgt ttttcgtttc ttatgtggga taaatgaaag taaaattgat
     4441 ttatcaagat cgaaagatct gttttgtgtg ttgaacttag cataagccac ttgtagtata
     4501 aacttcgcgc aatagtaaaa agtctctctt cataggggcg tgatttctca caagcaacca
     4561 cggttggctt gtgttttgtc acatctgtat atcggggatt gagtatgaaa ggacagaaac
     4621 agttttatct gcttcagtgt tctccgcatt ttcatcagtc ggttctgttg cagttatccc
     4681 ggagcggtat cgcttactac aatccagtta tacgcacttt ctataagagg cgtgactgta
     4741 gtgcatacag gggacaaatc atgccaatgt ttcccggtta tgtattcgtt cttctggatt
     4801 ttgaggttgt acatccatcc tgctttaccc ggatgaaaaa tgtttatgga ctggtcagtt
     4861 ttggtgaata tcctgctgaa gttcctctaa gtgtcataaa tgaagtaaaa gagcaggaga
     4921 agattttttc gatgaattta agcctgatga ataactcctg tctggcaaaa atacttctcc
     4981 ttgcagacgc ttcaaagcgt ggacaagtct ttgccgacta tgtgtgtaac agaaaatatg
     5041 acaggttaaa aatgacagct ataaaaggaa agcggaaacc gcaacgcaat gtactctacc
     5101 ttcctacaga agttcgtgtt gaagttgaaa aaattgcaat tgaaataagt ttcaagaggg
     5161 ggagacgtat ctctgattct ggttttgttc agtatcttat taaaaaatac aaatctcagg
     5221 cgatgaacga acttattcat ggggctgata ttcctgacga gtgatagcgc cccggtgttc
     5281 tgaatgtgga atatttatgt tatctacttc tacttttctt gcgcttgcca tgcaatgcgc
     5341 tgccagcgtt catcctgaca caacgcacga agtcgccagg gttgaatcag gttttaaccc
     5401 atatgcgatt gcggaaataa taccaaaggt taaacgcaaa cctggtgata aaggcgtagt
     5461 atcttacttt cctgaatcaa aggaggcagc acttaagatc gttaaaaaca ttgaattacg
     5521 gaatcatcgt tactctgtag gacttatgca aataacgagt accaattttg caaagtttgg
     5581 tacaacagca gagaaaatgt ttgatccatg cgaaaatctt aaggtatcag aaaaaatact
     5641 ggttgactgt tataaacgag gtggcgactt agtgcgtggg ctgagttgct attattccgg
     5701 caatccagaa acaggagtaa agccagaacc tgaatttaat aatacaagtt atgtacaacg
     5761 tataggattt agccctcctg ataataaaaa aagttttatt gttccctctg taaaggaaat
     5821 gattaaaaag gagaataaga cgactatcac acctgaagaa atcattatat atcctcaata
     5881 cgccatgcgt ggcactgtat caaatgaaaa ggaaacaaaa gatgttgaaa ttaaatctga
     5941 ataaacgtta tttaacgctt tctctattta tggctgcgtt gatgctttgt gttgcagaac
     6001 ctgcttttgc ggatgatgtg tctacgaaga caactggttt tttacagaaa ataattgatt
     6061 ttttaacaga tattcgtaaa cctgcaatta caattattgc tcttgttatt gggtatattg
     6121 caatattttc tcgtcaacat acctcatgga tagtcccact tgttatcgga attatcatct
     6181 ttattgttgc accatatatt cctgactggc ttgcgtaatt aaaggatggg cgcattatga
     6241 gtactctttt taaaggtctt acgcgccctg ctttaataag ggggctgggc gttccgctct
     6301 acccctttct tggaatgtgc attatttgtg ttctgcttgg tgtctggatt catgaggcta
     6361 tgtatgccct tattcttcct ggctggtatg ccatcaggcg tgtaacacag tttgatgagc
     6421 gcttttttga ccttctgtat ctgagaactc ttgtcaaagg gcatccttta tcaaacaagc
     6481 gattcagcgc agtccattat gcggggagcc agtacgacga agttgatatt tcaaaagtgg
     6541 ataactttat gaagctgaaa gaccagtctt ctgttgaaga gttaattccg tactcttcac
     6601 atatcactga taatattatc gttacaaaaa accgggattt gctggcaacc tggcagatag
     6661 acggtgctta ctttgagtgt gttgattctg aagatttgtc aattctgaca gatcagctta
     6721 atacgcttat acgtagcttt gaagggaaat ctgttacgct ttatcctcat cgtatcaggt
     6781 gtaaaaagga cgtcagacca gtatttaaca gtaaaattcc ttttgtgaac agagtaatga
     6841 atgattatta cgagtcattc cctcagtctg aatttttcga gaataaatta tttctgacga
     6901 tttgttttaa accttttact acggaagata aagtaacaca tttcttttca cgcagtaaaa
     6961 aacaaaaaga tatctttaaa gagcctgtta atgaaatgaa tgaaatttgc gacaggttga
     7021 atacctatct gtcccgtttt cattcccgac gtcttggact ttatgaagat catggggttg
     7081 tttattcaga tcagttatct ctgttccagt atctgctttc tggtcgatgg caaaaggtca
     7141 gggttagcag tagtccgttt tatggctttg ttgaataaat cagatttcgg gtaagtctcc
     7201 cccgtagcgg gttgtgtttt caggcaatac gcacgctttc aggcatacct gctttcgtca
     7261 ttttgttcag cgctcgtacc agggccatag cctccgcaac ctgaccatcg tagtcacgca
     7321 gcgtcagtga acccccgaac agctgtttta cccggtacat cgccgtttcc gctatcgagc
     7381 gacggttgta atctgttgtc catttccacc gcgcatcact cccggtcatt cgctgattag
     7441 ccactgcacg gttacggtct gcatattcac cggaccagta acccgcacct tttcggggag
     7501 ggataagcgc gctgattttc ttacgccgca gttcatcgtg acagagccgg gtatcgtaag
     7561 cgccatcggc ggcggctgac ctgattttcc ggtgggtttg ccggattaac ccggggaagg
     7621 cctctgagtc cgtaacgttg ttcagcgaca ggtctgcaca gatgatttca tgtgttttac
     7681 tgtcaacggc gagatgcagc ttacgccaga tacggcggcg ttcctggcca tgctttttga
     7741 ctttccactc gccttcaccg aagaccttca gcccggtgga atcaatcacc agatgtgcga
     7801 tttcacccgg gtgggcgttt tgaaactgac attaaccgac tttgcccgcc tgctgacaca
     7861 gctgtaatcc gggcagcgta gcggaacgtt catcagagaa aaatggaatc aataaagccc
     7921 tgcgcagcgc gcagggtcag cctgaatacg cgtttaatga ccagaacggt ggtgattgca
     7981 aggtcagaat agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
     8041 tgaatagctt catcatccag ccagaaagtt atggagccac ggttgatgag ggctttattg
     8101 taggtgggcc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
     8161 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagcctc
     8221 ctggctgttg tacatcgcca gtgctgcctt tagaacggta gttttgctct ggcctgttct
     8281 gatagcttcg acttccagga tttcttcata ggcttcggtt agccggaaat tcagtagtct
     8341 tgcttttgct gtcaattgct tactgttttc ttcaggaata tggcttgccg aaggatttag
     8401 ttttgatgtg acttctgtta ccacctcttc cggtaacgct tctaatagcg ttttcagcgc
     8461 atcaacgacg tttttatcca tgatattcct cagatattat tcgttctctt tgagccattt
     8521 atctacaagc tggttcacca catctgtgat cgatgtacct tttttggcac atgcagcctt
     8581 aaaccgggtg tgtttttctt cgtcaaaatt gacgttaaca cgtttgattt ttccagatga
     8641 tactggtgct gttactactc gttccagatc tctgttttca ccaaaggtca tttttttact
     8701 gccgtatgcg ctttttcaag tgacattaag gttactccgg ttgttatact tatttgatta
     8761 tatgagtgaa tgagtaatta ctcaaatgta ctaactatct cttttgtaag gatttctatt
     8821 tcaccttttg ctgcgccatc actggattca aacacgctgt caccatccag aattgatttc
     8881 acgtaaacct gacgttgtgt aatggccgta cggaaagatt taacaccagt gtctttgata
     8941 ctttctttca gcacattgag catggttgcc atttctatct tacgggtgat cagaaagcgg
     9001 gcttcaactt tgcggctgta agcctgtgct tccagaacag taacgacgct tcctgctgcg
     9061 gagaaatcca gggggctggg tgtaacaggg ataattacca gatcgcttac catgacggct
     9121 gcggaggtga ttactgagag cgaacctgcc ccgtccacaa tagcaaagtc atagtccgcc
     9181 agatcttttc ggattccata gacgtctttt tcagatgcag ctgtaaatac gtcaaatgct
     9241 gccttgcccg ctttgctcca gttcgtcagg ctcatttgtg gatctgtatc taccacagca
     9301 atgttgtatc cgcttctgct caacgcagtg gctatgttga ttacggcagt tgttttacct
     9361 gaaccccctt tcggattcag aaatgagatt actttcatac tcaaaccctc aattgtgtat
     9421 ctactcaatt actcttgtga gtttttgagt ttaccatact ctttgcatga gtaaaggtta
     9481 atatgagttt atgagttaaa gagcatatac tcatttgagt ttgttgtgta tatgagcatg
     9541 tgagtttatg agtatgggtg acgatgtcac atttactcat tatttgtttt ttcggttctt
     9601 ttagagtgta catcaggcac actggcggtc gggattatag cagtatgcgc agtcaccttt
     9661 tcactgttaa acgctttgct tttacgtgca gaatcaatat ccagcgactg aggaaagact
     9721 gatttcaggg catcgataac ctgcggcgct tcgctgaatt gttcatcaga aagatatgag
     9781 caatcagagc ggttataagt aatcgcttta tatttttcac gtaattgttg cgtaatatcc
     9841 agcgtttttt gtgccgtcat tttgaacttt ttgttcatgt attgctggag tctgaccagg
     9901 ttaaacggca acggcgcagc cgttttttta tcatcagttg ctgccgcttc aactgtagcc
     9961 ggttttcctg caagggatgc tgccgttccg tctgcccacg cgttatcaag taatttacgg
    10021 tctgtcagcg gagcaaattc ccctggcttc cagttcgccc tgataacatc agcaccacgc
    10081 tgaaagactc cggtcatggt gtagtaaaaa ctggatttat ggttctggtt agcacgggta
    10141 cgattcacaa tcaggccaag aacgggcgtc tggacgcgcc cgacagacag aacgccctga
    10201 tatcctctgg cttttgcagg aatggtgtaa gcacgcgtca tggagaaacc atagacggca
    10261 tcagcaactg aacgcgccag cgccttaagg taaagccctt tgaaatcacg attatcttta
    10321 agatttgcca gtgccttttt cactgccgga agcgtgttgt cgttaatcag aacgcgcttt
    10381 acgggttttg tgtttcctgc atattccagg acttcatcaa caagtagctg tccctcatca
    10441 tcaggatcgc cagcgtgaac aatttcagtc acgtctccac gtctgataag ttcgagaatc
    10501 gttttaacct gttttgctgc actttcaaca ggctgatact tcacgggata aagacgtaaa
    10561 ggaagcgttt caaccttcca ggctttgtat tcaggattat agttttccgg cggttgtgat
    10621 tcgataatat gaccaaaaca gttagtcaca atggcgttat cactttcaaa ccagccatca
    10681 tggcgggtaa aattgccacc aagtgcctta acaatatcat ttgctactgc gggtttttct
    10741 gcaataaaaa gtctcattta cttatccttt aatattacac tatagataac ttaaaacaac
    10801 cacaaaaaga tcttgcctac gaaagatctt tttgtttata tttacgcatt atgattttaa
    10861 gtaagtattt cactctaata aaaaggtggg catgttacat aaaaaatcag cacaagaaaa
    10921 tgtagccttc gggcttgctg tacttaaata ctatcttcgt ttcaactcca aagagaattt
    10981 acaagatgct aatgtgagtt cggaggaatt gataagcaat ttaataaaca ttttgaaaga
    11041 tactaatttt gtttgcacgt cagaaatttc agccacatat ccaggaatag atgccttaga
    11101 caaagaaaat aaactaggcc ttcagataac acatacaaaa aatacttcaa aaataaataa
    11161 aacaatagat aaaatacgca ttaataaagt taacgaagaa attgacacct tatatttttc
    11221 attacatcgg aaaagcagaa aaaatacact atcaaagcac aatgtccaaa actaaatgtc
    11281 acaacaaata atatattaga ctttgatgac ctagctaaac tgttatcgaa aagtcaggat
    11341 aaactgagta aagcagaatc cgaacttatg aaagcaatgc ctcgtctcta ccaagaagga
    11401 aaagaacggt atcaagcaat gcttaataac attcttgaaa ccagaaataa actagatcga
    11461 agagtattca ctgctaacaa aatcttagaa gaacctgaag aaatgctgtt gtccctcaaa
    11521 gagatcagga tagatataca aaaagatggc ataatgaaca aagccaatcc tgctgtctct
    11581 gattcattta ataaaataat aaatattatt gatgatgtag agagtaaaat tgctgtacaa
    11641 tatcccgatg aatacaaaaa atataaagca aagatccttc catcgtggaa ttctccagaa
    11701 aaagaagaat gcttagatat tttaatggct ataagaaaag atgttttgga acaaatagac
    11761 aatattgata ttgaggtaaa taagctaaaa agcattcttg ataataacat ttaggttaat
    11821 taatagctta tttaccgtcc attagcatga gataaagtat aattagcggg caagatagat
    11881 gcccgcttct aaaataatat tcctttggcg tgggcatcag gttttaatta acctctgaca
    11941 cgaccaaact tactcatgat tttcgatata gcagcaggat cagccgttga acactgattc
    12001 agaaatgctt tacgggcatc aaaagtttta ctccagcgaa tgcttccttt tttctttttc
    12061 agaatattaa aaaaatcctt ttcagcactt ctgcactcac tccctccacc ttgcccgact
    12121 gcttttccat ataagcaaag taccgaagcg caagggtcag atgcatacgc aggtgctgtc
    12181 agccaggaca cagtaaaaaa tacgattaga cttttgcata tctttttcat ggtaacctct
    12241 gaacatttat tctcgaaaga tagatttcaa catagtcatt attcaaccat cttttagtgg
    12301 ctgatattca ataacacctg ttgcaccatt ttttcttagc cagtctttcg cagaccagta
    12361 actatcaaaa gaagatgtcc ttacaactat tttttcagag tggacaactg cataaaaaac
    12421 agaatcatcg taaagacgat tttctggttt aaaaccaggt aacaccttac tccatgagga
    12481 atttatatta tctgatttaa taacgttgga tgttggtgtc cagttaatta tttgtgtatt
    12541 cataacttca ggccgttttt cccattcaac ctgtggcggt tcaggtggag aggaacaggc
    12601 tgtgaggaaa agaatactga tcataagtat ttttttcata ataaaacctc agactccagt
    12661 tctcctacgg agagcatttt ttcttttgac ggatatttaa gccctttcac accaaatatt
    12721 tttttcgtct tatttagttc catcgtcaat gatgccagtt taggactgac cttcattaat
    12781 ctgtccataa aatacggatc aaggtaataa agtgcctttt ctgctttaac agggttctcc
    12841 cccttcagga tgataaactc ttctttaaag tccagcgttc ccagctcctg tggaagcacc
    12901 agtgctcttc gggcttcact ttctgattcg ccctgtgatg ttgaccgtcc acggctcttg
    12961 cttgtgctct ttgatgtagt ggtaatatat ccaagttttt ctgatatctt cgcggcatca
    13021 tcctcttcgc tgacagcata gataatacgg catggatgag cactcatcag cgttttggct
    13081 ccctcaatac catagatttc atttagctga ctgatattct gataaatcgt cagcagttta
    13141 agtttaaaac ctgcaatata ccctgatccc tttttaataa ttggcatata accaatcgaa
    13201 gggaactcat caagaaacat caggcagtca tgtttcaggg tgggatcaaa atcaggattt
    13261 tcacgcaatg tcacttcaac aacgaagttg aaaaacaggt tcagaaaatc gtaagccaga
    13321 gatatatctt ccgcattaac accgacataa acagtgatat cttcccgtcg caactgacgg
    13381 agatcgaaat cattaccatc agtacattta cgaacggttg ggagataaaa caggctcatt
    13441 ttcttacgga aagaaccatc aatgcttgaa cgctgttcat cttccgttcg tgattccctg
    13501 attttggtca gggcatcgcg caaatgatac aacgcgtttt catcaagccc gtttgttccc
    13561 tccagttctt cccgcttact gagtatcagt tcccggtcaa tattgctgta caagtcgacg
    13621 acagaaccga ttgagaaaac gggtttaagc ccgaactcat taagccagga cggctcatag
    13681 ttaataaaga aatgaagcaa cttagccagt cctgtccagt attgacccgc aagattatta
    13741 aagtgcgctt ctgcccctgt catgccataa gacggaaaca gaatttcaat cagtttaagc
    13801 agatccttag ccccactctc cgcttttaaa tcaatataga aaaggggatt aaactggtgt
    13861 gttttactgt tgaaagggtc gagcagataa actttattac ccagcagtat ttcacgcacc
    13921 ttactggtga ttttccacaa ttcctgtttt ggatctaacg caatcagaga gtgttttctg
    13981 accagaagat tagggatacc aatggcggca cctttacctg cgcgggttcc tgcgccaagt
    14041 gatacaaaat ctggtgcggt ataccacagg tatttacctt tatatgcccc gacgagaata
    14101 ccagtatcgt tttctttctc ccatttcaga agtttcgatt ttcttaaatc attatcactg
    14161 gcaaacttcg cgtcaccata aagagcaaca tctgttttat tcagttgcca gataataaac
    14221 acaggcacga tgagagacga aaccatacct gataaaagag aggggatagc ggttaacctg
    14281 atatctgtcc gtatattact ctctgttatt attcgccata ggagcatgga atcaaaattt
    14341 ttccatatat aaagcggcgt ttttccgttc aggaagtaaa cagcaacaga accagtataa
    14401 tatgtcacga gacacataac cagaccgata aaagcccact ggcctttatc tgggagtttt
    14461 aaagacataa tcactccttc agttttctga aatagtcatc aaaatgaagt tgtttgtagt
    14521 aaataccgga agcgaaccga aaatcttcat catcaaggta tttaatactc atgataatat
    14581 caatattact cagtacacgt cttaaaatga cattgaatgg tagattctga cattcaggat
    14641 tcatataaca tcgctggaca atcccaagca cggcagattc aggactactt tcgtgaacgg
    14701 tggtgatgct tcctgcatgg cctgaactcg atgctttcag aaatcccatg cctcagcccc
    14761 cctgatttct gtcatcagaa tcctgtccgg attcatacga aaacaggaac gcagaagact
    14821 cgctgaggta ataaccttac tctcaccctc tgccggatag taaagtttta catggttttt
    14881 atggaatctg aattttgctt catcagtatc ttcaatagaa atacaccgca gatgatgcgg
    14941 tatatattcc agacaggcat ttgcaaaggt agttttacca gaccccgtcc ctccacagaa
    15001 aactatcgtt ttcccctgac tcagtgattc aggtataacc cgttcaaaat attcggaagt
    15061 gattaaagca gaaatattat cttccataat ggaatcacca cttaaaccac cgacgcgtga
    15121 ataaaacccc tgttttataa atttgtcatg actgataaaa atacctgacg gcttacggat
    15181 tgttatagaa acagtatctt tttctgttgc cggtgggatc acaacctgaa cacgttctcc
    15241 acccggaagc gtggcggaac gcaggggata ttcaggagtc acagaaccac cgtcatgaaa
    15301 atcagccagt gtggatgcaa aagaaagaca atcactgtaa ttcatctttt gttcatacca
    15361 ttgccacttt cccctgacct ttataaatat ttctcctggt cggttaacag cgatttccgt
    15421 gacaccatca atctcattca gccagtgata aaaataatcg ttgaccacat cataaatcag
    15481 atgtctgttt tcgttattca cattaacccc ctttcatttt cagtttataa atacctgaaa
    15541 aatccagatc ctgaccgaca atcagagtaa taatttcgcc ctgattttta taaagcgttg
    15601 gcggaatatt cacactatta gaaaatgctt cgcgtgctat atcagcaaaa gcctggcgac
    15661 tgtttgccgt atagtcggtc tggctgtccc tgttgttctg tgcaatacca ctggcggcct
    15721 gacttaaatc cggtatcatc ccgaccatca ttgcaccaag aaaacgttca ctgaaatgag
    15781 tgtcaatcca accagaagca ccggcttcgc ctaacgcgcc tgccgcctgt gagtcaacca
    15841 ggggaatatc aataaaaggc ttactgcgag tacgtagctt gtaagccata acaaacacac
    15901 gcccttgtcc atgatttaaa gacccggcct tatacatcag ttttgcggcg gttcctcttt
    15961 cgataagttt tacattaccg ctggcgctgt atatatcgct gttgaccgta cattccagtt
    16021 tccccgccag gtcagaaaca aaacgcctgt ccagtgaaca gggaattgat gtcccttccg
    16081 ggataaacaa atttgggtca tatggaagtt tcgttatttt cgccagtggc gcagattctg
    16141 tagcctgtgc ggttgctttt gcttcatcct ttttatcgct ttctggctca ctggccgcag
    16201 tatgtgaaac tgacgttccc gctgaactgc tgctgtttcc tgcctggctg ccatcaagag
    16261 aaacatcaag tgcacggctg aagctctgct gtttattttc cggcggttca gtcttttcag
    16321 aagaaatgac tttttcttcc tctggtttct ctgttttttt ctgtgcggta ttaaaaggat
    16381 cgatatcttc gctcagtccg gtctgacgtt tagccgctcc ggttgcaagc ggctgacttg
    16441 cgggtttctc atcccctgac gacacagaac ggataatgga cgggatataa taaacagaaa
    16501 caccggcagc aagcagaaga aaacccacga cgaggataat catcccttta cgtgactttt
    16561 taagtttttc aataccaagc tgcaatgctg gcttgccagg ctcttcttcc ggagtcttcc
    16621 cggcctgttc cagttctttc tgtctgcgtt cccgtagctg ctgttcaatt tcggttgcag
    16681 tcgggatttt attttcctgt tcagtcactg gatcacctct ttcttaacat ccggggatac
    16741 cgtatccccg gctggtaaac gtacttttcc gaagcccagg ttctcaatcc ccaccacctg
    16801 ctcaccaaga cgtaaaacaa aacgcttatc aaccggtacg ccaacagccg taaaactccc
    16861 gctctgttta atcacaggat tggtaagagt ctcctgcata ccgttcatca caaagacgct
    16921 gggaattttc tttaacggac tgaagccgaa ccaggtataa cgaccatcgt catatgcata
    16981 atcagggctg atatcctggc tgccttcagc aacccgcttc cagtactgcc agttacgggg
    17041 cgatttcgca ttttccaggg aacggttgat tgcctgttcc tccaggcgtc tggcaagagc
    17101 ctctgtgcgt ttcttctcaa tttcggcctg ttccttccgg cgttcctgcg gatactggta
    17161 attcaccaca aacgcaattt tctccggctg tcggaacgtt ctggcgttca gctccatgct
    17221 gtaattacgt ttcgtggtgc gaacaaacag attcgttcgc cagcgttcaa gctcattttc
    17281 cgggtcaaga gcaacactga cggattcaga agaggtgtta ccgtttgcat ccgtattatt
    17341 tttctgaaca gtttgtttaa caggacgaac ttccaggtat accaggttat cttctttatt
    17401 aaccgcccat ccctgcggaa aaccggttct ggcactgata actgtttcat cttcatcaaa
    17461 taccagtgtg gtggtgtatc caacggcact gttaattatg gtggtattat tggggttata
    17521 actgactatc tgattgcgtg ggtcaaagcg actgcctgaa ggaagaacag cagcattaca
    17581 gacgtgcgcc gccatcagaa gcgccataat taacgctcct ttattcattt tcatattatt
    17641 aacctctggc ttctttatcg cgaacgtagc ttgttacaac gaacttaaga ggattgttat
    17701 tacgttcccc tgacaccatt tcaacctgcg gttcgatacg ataagtcagg cgaacattcc
    17761 agaactcctg gcgggtttgt ccggtagcaa catccctgat ggtcagacga aaacgaatat
    17821 acgcaccgat atctttatca tccgtccgtg acgaaggtga aattatgaca gatggattat
    17881 cctgaaccat tgccgttttt tctgctttgt tataaacaag ttttggtgcc tgttcactgt
    17941 taaataatgc gttataatct gccgcgacat tctccgcgct gtaagccatt acataatcat
    18001 aatcatgctg gagactgaaa aaattataac cttcacgatg ttttatatac tgggtgataa
    18061 aatatcgggc aatggcttcg ttttcagaaa gattttcttt tttaacagaa gtcagacgtt
    18121 cagccacacc tgtaacctta tcgataaggt aggcatcaac aactgttgtt ttcagtggta
    18181 atgctgcatt taatgcaagt aaagacagcc caccaattaa catgcctccg ataccaccga
    18241 atttataaat acgattcatt ctctcctgta gtgcaatgtt tttttcttca aacgaaactg
    18301 atgagtcagt aacgtctttt ttatttttaa atccgggaag tttaaattcc attttgcatt
    18361 acctcagcag aattaacagg ttcgcttttc ccggaaacgg gcggtagttt atgtgacgcc
    18421 tgacaaccag cgagacaaag cgtcacgaaa gccacaataa gtagtttcat aatatgaaca
    18481 cctgtataac agattaatat ttacagaaga ctcagtctga acaaagaaaa tagatcgcga
    18541 caagcccgtt aattatgaaa agaacattta aaatggtatc aacactcata attaaatcac
    18601 cctccataca ttttctttgc cctctcaata gctgcctgac gaagcttttt ggcaccaata
    18661 ttaacgccct gtccggccag gttagcggtc ttacccgtta ttcccggaga ctgaccaaac
    18721 ccgccttcct gacgacgcag acctttccat gcaccaatac cggcatttct gcccatacca
    18781 agtgcgccac gcgccatacg ggatgcaccg aaaacgccag caccaatccc catagcggct
    18841 gcgccctgca tggcaccttc aacacccaca cctgcaatct gtgaagcata acttttcgcc
    18901 tgccagataa tccaggccat gaaaatgcca gcaacagccg cagtagcgcc aatcttaatt
    18961 agattacgat tgtctaccgt atctattgcc agagataaaa ctatattgag aaatgatgtt
    19021 ccggctctta aagctaatga accgaacata aatatcaagc atgaactgaa aataagctgc
    19081 aaccagctat taaacatttg tctcaaaaaa ccaaacatca ggcaaaagat aaaaacaggt
    19141 gcagtgacag tcaataattt cagggttact tccgcagcaa gaaaaactat agcacatgca
    19201 actaatgcta atattccacc aaaataagta aaaagagcag ctatagcgcc atctgttttt
    19261 acataagttg aagtatcctt agacataaca tgcgcagcaa cctgttgtac tttcgtccat
    19321 aactgatcta accataacca agcatcatta ttaccagata acgaatcctt taaaccatct
    19381 attgctacca gggaagtatt tagccaccca cctgcatttt gtacaaacat taaaattata
    19441 gaaaacctgc acaagttcca gataaaatca ggcacaggtg tttgctgttt tctagccaga
    19501 acagtaaaag cataccataa cacatagatg gacaccccgt agcgtcctag ggtgcctgct
    19561 atattactta aacttgttaa ctgaccagaa accgaagcat cgagaaccga gaggatctct
    19621 tcttttgctg tgacaaaaaa attacttgct tcagccatct cttgtttcct tattttttag
    19681 gcacatcacc ttttcttcat gattagaagc atctttatat tttttaaaat actctttaaa
    19741 atttggttct ttggcatgaa gttgttgata acggaaatta gcttctttta catttcgaca
    19801 attctggctg tcacttccgg ttttcttaca ctcagcttct tttttcaatg attcttcatg
    19861 atgctccaac caccaatcaa cagatttggt ttcctcacat ccgacaaggg aaaaaatact
    19921 gagtgcacca atcaaacaaa aaactttttc ataagacaac ctctcttcag tttaaagtgt
    19981 atcttctcgc tgcatcctta aatgaaggtt cttcagcctc tagttgttgt tttctgaaag
    20041 cagcctgagc catcttctct ttatattctg caagatctcg ttgcttatcg cgatacattt
    20101 gatactgaaa tttgagcttc tcgaacttca gttgttcaag tgcaacagcg tttgttgcat
    20161 cctgcgtggc cttcgtatcc ttcgcatttt tgacttgatc aataaggcct ttcatcgtct
    20221 ggttgtcctg acgaagcttg ctctccagct tgttaccgta ttccacgctt gccagttgag
    20281 aaaggaacgt cgcttcacat ccccgtacca ggtcgccgga atatcccagg ttctggcagg
    20341 tatccgttac tttgtaatct gacaataacg atttggcctg ttccgataac gccccttcag
    20401 gatttttaat gtaatcgtca ataaaagcat tcccctgatc ataaatctgc atcagttcct
    20461 gactcactga ctgtgcggac tgcaccagtc cctgcacatc acggataccc gtttttgaca
    20521 gcagctcctg tttgtacgca tttatctgat tttcgtaatg ttttaccgtt tccgcccatt
    20581 gcttaaggcg ttctgtccat cgtccggctt caatcgccca ttcagggttg gcgtcgatgg
    20641 caacaggtat ccctgcctga gcgccggaaa taacaatcag tgaagcgacg aatgccgcca
    20701 taatttgctt tttcattggt gattcctcaa atcaggttcg cctgttccag ataggtatca
    20761 agccactctt ccggcttcat tccttctttc cagatttcat cgaacagttc taactggggt
    20821 tttgatgcac tgagaaccgg taagtaatac gctgcttttc ccagctccag tctggcaaaa
    20881 gcagcaaaat catcactttc acctttcctc tgtgggttct taacaacaag gaactggcgg
    20941 gacagcggat cgatagcttt aattttgtca aaataaagct ccctgacctg caaaccatca
    21001 acatattcac tacgtttggc tttcgggttt gccagataga tatgagtggc gcattgctca
    21061 cgaaccgctg ccgcaatggg tgatttaatc agttcatccg gtgactgtgt ggctacgaca
    21121 agcaccatat cgagtttacg tccggttttc agcttgttgt aaacaaagtc cctgaacgct
    21181 tcgttattga tccattgcca gaactcatcc atgtagataa gaagcctgcg accatcggcc
    21241 agcatagtga cccgatagat gagatagaat gaagcagcac tggccagcac cttattatcc
    21301 aggaattccg ttccatcaat accgaaaaca tccaggttat cgacatcaaa cgtgtcttcc
    21361 cgattgtcga acacccagcc aaactcttcc cctgcgtcca cggcttaaga cgggctttaa
    21421 gtccatgacg tttggtttca gtatcatccg gctcctggat aagaggccgt agtttactga
    21481 taggataaga gcgatctgac cgttgcataa gacgttcaac cgcatctgaa atcaggctgt
    21541 tctggtaatc atcgagcggc tcactgttga gagtacaaag cagcctcacc aggtccttca
    21601 taaaagcgat attacgtttt gttggcggca gtgcggcggg attccagcct gtcggctctc
    21661 cctccttcac ccggtaataa gatcccccca ttgcccgtat accgacttca cctgcccggt
    21721 ctttatcgaa aaaaaccgtg gtcagttttt tcagttttct gtttgcaggg aatgattccg
    21781 gcgtaccgaa ctgctgggcg gcaaatgctt ttgtcatcag taatacggtt ttacccacgt
    21841 tagacgtacc gagaatttcg gtatgcccca gcgtcgggtt cttaccgaag aaattctgat
    21901 gttcagtcgt catatggtaa ttcagatggt agatatcatt acctgacccc cgaagagtaa
    21961 tcagtttttc cccccaggta tttccttttt cttttcctga aaagaaatta tgaaaacttt
    22021 ccatttcagc aaaattaaga ctactgaggg tactcagacg agggcgaagc gtataatttc
    22081 ctggtagctg agcaaaataa gctgcgccaa gactcagtgt tgaataagtg acaatcagcc
    22141 ccaaatcttc cagagtggaa gtcacgatat tggtgtcttt caccagacgc tccggtgaat
    22201 ccgcaaagac aaccagggtc tgatgcgatt tcccgaaaga aatatatcca ctggaaacca
    22261 tatccagtcc gactttcaga tctgccagca aagatttggc agcatcatct gtcatttcca
    22321 gcttatcgat ctgatcatcc agcgccttaa tcgctgactg cttatccatc gcagtaaagg
    22381 acgatgtcac gacatactca acggggagat acatcagagc atccagaata ccggcatccg
    22441 tctcctgaaa ataatcctta atctctatgc aacgaaaata ccgggcatgg tctgacgcgg
    22501 taatttgtcc ggcatcatta ccaaagaaca ggtcttttcc tcccagatat gtataaaacg
    22561 gggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
    22621 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gcccacctac
    22681 aataaagccc tcatcaaccg tggctccata actttctggc tggatgatga agctattcag
    22741 gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgcta ttctgacctt
    22801 gcaatcacca ccgttctggt cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag
    22861 ggctttattg attccatttt ttctctgatg aacgttccgc tacgctgccc ggattacagc
    22921 tgtgtcagca ggcgggcaaa gtcggttaat gtcagtttca aaacgcccac ccggggtgaa
    22981 atcgcacatc tggtgattga ttccaccggg ctgaaggtct tcggtgaagg cgagtggaaa
    23041 gtcaaaaagc atggccagga acgccgccgt atctggcgta agctgcatct cgccgttgac
    23101 agtaaaacac atgaaatcat ctgtgcagac ctgtcgctga acaacgttac ggactcagag
    23161 gccttccccg ggttaatccg gcaaacccac cggaaaatca ggtcagccgc cgccgatggc
    23221 gcttacgata cccggctctg tcacgatgaa ctgcggcgta agaaaatcag cgcgcttatc
    23281 cctccccgaa aaggtgcggg ttactggtcc ggtgaatatg cagaccgtaa ccgtgcagtg
    23341 gctaatcagc gaatgaccgg gagtgatgcg cggtggaaat ggacaacaga ttacaaccgt
    23401 cgctcgatag cggaaacggc gatgtaccgg gtaaaacagc tgttcggggg ttcactgacg
    23461 ctgcgtgact acgatggtca ggttgcggag gctatggccc tggtacgagc gctgaacaaa
    23521 atgacgaaag caggtatgcc tgaaagcgtg cgtattgcct gaaaacacaa cccgctacgg
    23581 gggagactta cccgaaatct gatttattca acaaagccga atcatactaa taaattttcc
    23641 agcttatcgc tggtaagcaa ttctgccagc gaatcacatt tcataaaatc aatatccatt
    23701 tcgtccagaa catcacagta gctgacatca tcaagactaa tgacaatcac aggaacaccc
    23761 tctttgagat caaacactaa gacaggtaat gaaatggagt ttatcttttc aaaatcacta
    23821 aaaaactgtt cagttccata ttcactggca ggaaaaagaa tctcacttaa tctcacacaa
    23881 taaaaaacat gatagtgttc tttaaggtta tcctttaaaa tattaacagc cttagtatat
    23941 ttttcgttaa taaactcttt gaccaaatat ttcataaaat tctccaacaa gaaccgactg
    24001 taggtcaccg ggcaaacgtt gcggaatggc gtcagagacg tcattttgcg gcgtttgccc
    24061 tatcctgcat cgcagtgaaa aggcagggac tggcataact gctgagtagc atcattgctg
    24121 gcttatctcg ctactgttct cggtgctttc gtccgcaggt tcgggaacaa gtaacttgtg
    24181 ccaaggcata gttttgttga gcagctccat cagaggctca gccagcagtc ccaaaatcca
    24241 ggttgccgta atcaccggta tagtcagatt ttgtggcgtc cataactggt gatcgagatg
    24301 ataccaagtg atgacacaca aaataccacc cagccagaat acagcccatt taaacgcacc
    24361 aagaatggct aacacaaaaa caaagataaa atgtaaaaca ctcgcgacaa gaagacgaag
    24421 ataaaaccat ccccagccaa gccacttaac aaaagtgctc cctgttttca ctgcaaaacg
    24481 tttcttacgc tccctggcaa tttctttcct gaatttagca cgttcttccc ctgagggaat
    24541 tgatagattt tagccatgag ctacctcatt tcgatctcaa aacgaagttt atgcaattat
    24601 cctctgaaac cgtatggtat tctgatatct aagccctcac tttctttatc tattttataa
    24661 gaaaacctta gctttgatgc tcttcttgcg actctctcaa cgattttaac ttcgagatga
    24721 ttaatctctg tatattgatt tattttttca atattttttg cagaaaaacc ttattaaaat
    24781 ccttaaaatt ctgataaagg taaacttttt cgccattatc tattctgaat aattcaagtt
    24841 catcctttaa tacatctact tcaatgtcga aaaatgtctt tttaccactg ctgtattgtt
    24901 tcctgataaa ctgataaagc atatttgaat tttgatcagc gagtcttaca gacgatatca
    24961 aaacctgagt agtgtaagaa tcctttaaca tcgtgatata tggctcaatg acatgtgaaa
    25021 aagcaatatc aatatatcct tcactttcaa cataatcaca aaacgcagtc aggttcataa
    25081 gcctgactgc atcagaaggt aatttacctc tcttcttcgt atgtccagag ctttgattgc
    25141 cctgaggtgt gagaagttga tttcgaggaa tgcgaataac actggcctga agttcttctg
    25201 ccccctcttt caactgccgg taagcacctg ttacatcaat atcagcgata aatgcatatt
    25261 cccgagccgt aatacgaaag gttgccccct cagataactc ttcacgtgaa tcaatctgcg
    25321 ccatagccat aaacaaaatt cttctggcag acaaaggcaa cgatgagaat gtgctgttaa
    25381 tttcatttct gtggcgaatt tttgttttct ttgtaacgct cagtaagttc atacattacc
    25441 tgtggttttt aagttctgat ggtgaaatca taaccacaaa ggcggaaata tgtaaagtgg
    25501 tttatccacc ttatgtattt ccaccaaaac acggatcaca tgaaagaatt tttatcattt
    25561 atattcaata tgatagaaat gcgcaggtat ccacctttta acgtcatacg ttaaattaga
    25621 gcactataaa gaactaaaga aatgaagata caaagatacg aatatctgaa taaaaacaga
    25681 aaatgcgtac ccatccacct ttcagtgcgt acccatccac ctttcagtgc gtacccatcc
    25741 acctttcagt gcgcagccat ccacctttca tgaaaattac agtgcgtacc tatccacctt
    25801 tcagtgcgta cccatccacc tttcattttg aggcgacaaa aaacaacacc tatcttaatg
    25861 aaattattga taaaaaagct ggcacaatgt ttgcaaaata agttaaagta agttaaagat
    25921 cttaaagtag atctaccgat ctacatttaa gatcttaaaa acaagaagtt taataagcaa
    25981 aaaaacacaa aaaatctgtg gataactctg ctcaaaacaa aaaattcaca gcgcctgtgg
    26041 ataactttgc gtaaacccgg aggacagatc actctgaacc aagacaacca cacataaaaa
    26101 caatatgctc attttttaac cactgtgcgt aagtgcctaa tgtaatccct gtgctattcc
    26161 gcttcctcac tcactaactc gctacgctcg gtcgttcggc tgcggcgagc ggtgtctcct
    26221 ttctcaaaac cagaagagtt cagctcatac gatccgttga ttgatccttt ttggatcttt
    26281 cactgagaag ccctgaatcg ctttctgccg cttcgttcga tacaaccatg gtgaaaccat
    26341 gcgaaatcga aaaaatcgct cagaatgcga tacagcgcgt tttagagggt atttacaaaa
    26401 cagtcgttgc cttgaggaaa ttttgatcaa gattgaactc ctatccggat catggcaaca
    26461 gatcacagca acaaggccgc taaaaagcgt tgagcggcgt taaaaaccgt taaaccgtag
    26521 ctgactacaa cgaaacggga aaaaacgctc agaacgcgtt acagagcgtt ttaaggcgat
    26581 gtcggaaaga gccatatctc acctgttctg atgtttttct caacataagc acttttaaga
    26641 gtgcttttaa agatgctatt atgccaatga gacaagcgga gggcttatga gctatcaaat
    26701 tctgacaacc acagcggcca gtattactga cctgaaaaaa aatcctatgg gaaccgtagc
    26761 tgaaggtgaa ggggacgctg ttgcgatcct gaaccgaaac gaaccggcgt tctattgcgt
    26821 tccaccaaaa ctttacgcct actatcggga actcgctgaa gatgctgagt taaacgctgt
    26881 tgctgatgag cgcatgaaaa acccggaaat tgtgaaggtt aacctggatg acctatgaac
    26941 tggcttttga ccgtagagca ctgaaggaat ggcagaaact cggccacacc atccgtgaac
    27001 aattcaaaaa gaaactggca gaacggctgg aaaatccacg cgtacccgca gcccggttac
    27061 atggtcatgc tgatcgctat aaaatcaaac ttcg
//
