LOCUS       NZ_CP044048             1750 bp    DNA     linear   CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_629 plasmid unnamed5.
ACCESSION   NZ_CP044048
VERSION     NZ_CP044048.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056344
            Assembly: GCF_008693445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3
            Genome Coverage                   :: 814.56x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:40:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,493
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 65 of 145
            Pseudo Genes (incomplete)         :: 81 of 145
            Pseudo Genes (internal stop)      :: 23 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044048.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_629"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_629"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MI"
                     /collected_by="University of Michigan Health System"
     gene            258..1229
                     /gene="mobQ"
                     /locus_tag="FOB37_RS28515"
                     /old_locus_tag="FOB37_28520"
     CDS             258..1229
                     /gene="mobQ"
                     /locus_tag="FOB37_RS28515"
                     /old_locus_tag="FOB37_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_150340979.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRAGEKLEDERTGLTHDFTRK
                     DGVAHSEIISNLDIEIDRGELWNLAEKTENRKDARTAREWVIALPDELDADQRKDLAKD
                     FARSLVDRYDVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDADKKLTLTTKTDIELS
                     NAKRKSLGMGTTQEDIKQIRETWAELANKALERAGYREKIDHRSYADQNNGLQATIHEG
                     TKVTQLRRQGIDTEISRFNDNVKQQNSQQLHQQKQQKESVLQRGLSRVDQGFDQWQKNQ
                     ETKRLELERQAEMKRQQELDKQRAEQALRKASQKLSRGGMSL"
                     /name="mobQ"
     gene            1226..1726
                     /locus_tag="FOB37_RS28520"
                     /old_locus_tag="FOB37_28525"
     CDS             1226..1726
                     /locus_tag="FOB37_RS28520"
                     /old_locus_tag="FOB37_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_150340980.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLEQFYNHVASGVLD
                     KAEKSLENANKKHTGALNNSIQALNEATHRLNLKLIIIFASTFVAVMMVFILAIFLYIP
                     SKDEIDARRADMAVLKKYPLQIEEHDGKTLVRIMTKKPCYSFEPNNVKEKTYDWCQIDP
                     KKY"
                     /name="hypothetical protein"
     Typing          259..1226
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaattgggc ttcgattttc ttttcaagag tttcagcagc ttttgtcatg attcagattt
       61 cgggatatag aaaaccactc aagcataagg ccctatgaat tgaaattcaa gcgggctata
      121 tgctatcgtg tttttcatga aatgcgcact tacgacttgg atttcatcca agtcaaagtt
      181 tgcgtaaggg gtatcccctt aaccccaaaa aactgctacg caatttttta aaagcaaaag
      241 catctcaagg cgactgcatg gcaatctatc attgttcaac taaaacagtt aaccgaagtt
      301 caggacgaac tgcggttgca tcaagtgctt atcgtgcagg tgaaaaacta gaagatgaac
      361 gcacagggct gacccatgac tttacgagaa aagacggtgt cgcccattct gaaattatct
      421 ctaatttaga tattgaaatt gaccgtggcg aactttggaa cttggcagaa aaaaccgaga
      481 accgcaaaga tgcccgaacc gctagagaat gggtaattgc cctgcctgat gaattagacg
      541 ctgatcaacg taaggacttg gcaaaagact ttgccaggtc cttagttgat cgctatgacg
      601 taatcgcaga tttagccatc catgaaccga gtaaaggcgg taatgataaa aaccatcacg
      661 cccatatcat gctcacaacc cgaaaagccg aattggacgc agacaaaaaa ctcactctga
      721 ccaccaaaac cgatattgaa ctcagcaacg ccaaacgaaa aagccttggt atggggacaa
      781 cccaagaaga cattaagcaa attagagaaa catgggcaga gttagccaat aaagcattgg
      841 aacgtgcagg ctaccgagaa aaaatagatc accgcagcta tgccgatcag aacaacggac
      901 tacaagccac catccatgaa ggcaccaaag tcactcaatt acgcagacaa ggcatagaca
      961 ctgaaattag ccgtttcaat gacaacgtca aacagcaaaa tagccaacag cttcaccaac
     1021 agaaacaaca aaaagagagc gttttacagc gtggtttaag ccgtgtcgat caaggttttg
     1081 atcaatggca gaagaaccaa gaaaccaaac gtctagaact ggaacgccaa gcggaaatga
     1141 agcgacagca agaattagat aaacaacgag ccgagcaagc actccgcaag gcatctcaaa
     1201 aactaagtag aggcggaatg tcgttatgag caaccaaaat gatttagatg accaattata
     1261 tattttactg gcatccatga aggaatatcg agaagccata gcagacgata acaagcgatt
     1321 agagcagttt tacaaccatg tcgctagcgg agtgctagac aaggctgaaa aatcgctaga
     1381 aaacgcaaat aagaagcata caggcgcatt aaacaacagt attcaggcat taaacgaagc
     1441 tacacacagg ctcaacttaa aacttatcat tatttttgct agcacgtttg ttgctgtgat
     1501 gatggtattc attttggcta tcttcttata cataccaagc aaagacgaaa ttgatgcacg
     1561 tagagcagat atggctgtac taaaaaaata tccgttacaa atcgaggagc atgatgggaa
     1621 aacattagtc agaattatga ctaaaaagcc gtgttactca tttgaaccga ataatgtcaa
     1681 agaaaaaacg tatgactggt gtcagatcga ccctaaaaaa tactagttga tcggtacttt
     1741 gcttggtttt
//
