LOCUS       NZ_CP044044             7819 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_629 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP044044
VERSION     NZ_CP044044.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056344
            Assembly: GCF_008693445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3
            Genome Coverage                   :: 814.56x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:40:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,493
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 65 of 145
            Pseudo Genes (incomplete)         :: 81 of 145
            Pseudo Genes (internal stop)      :: 23 of 145
            Pseudo Genes (multiple problems)  :: 22 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044044.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_629"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_629"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MI"
                     /collected_by="University of Michigan Health System"
     gene            complement(67..177)
                     /locus_tag="FOB37_RS00475"
                     /old_locus_tag="FOB37_00475"
     CDS             complement(67..177)
                     /locus_tag="FOB37_RS00475"
                     /old_locus_tag="FOB37_00475"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            461..1158
                     /locus_tag="FOB37_RS00480"
                     /old_locus_tag="FOB37_00480"
     CDS             join(461..710,710..1158)
                     /locus_tag="FOB37_RS00480"
                     /old_locus_tag="FOB37_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            1245..2237
                     /locus_tag="FOB37_RS00485"
                     /old_locus_tag="FOB37_00485"
     CDS             1245..2237
                     /locus_tag="FOB37_RS00485"
                     /old_locus_tag="FOB37_00485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            2426..3546
                     /locus_tag="FOB37_RS00490"
                     /old_locus_tag="FOB37_00490"
                     /pseudo=""
     CDS             2426..3546
                     /locus_tag="FOB37_RS00490"
                     /old_locus_tag="FOB37_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            4440..4631
                     /locus_tag="FOB37_RS00505"
                     /old_locus_tag="FOB37_00505"
     CDS             4440..4631
                     /locus_tag="FOB37_RS00505"
                     /old_locus_tag="FOB37_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4631..5029
                     /locus_tag="FOB37_RS00510"
                     /old_locus_tag="FOB37_00510"
     CDS             4631..5029
                     /locus_tag="FOB37_RS00510"
                     /old_locus_tag="FOB37_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5036..5734)
                     /locus_tag="FOB37_RS00515"
                     /old_locus_tag="FOB37_00515"
     CDS             complement(5036..5734)
                     /locus_tag="FOB37_RS00515"
                     /old_locus_tag="FOB37_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(5731..6618)
                     /locus_tag="FOB37_RS00520"
                     /old_locus_tag="FOB37_00520"
     CDS             complement(5731..6618)
                     /locus_tag="FOB37_RS00520"
                     /old_locus_tag="FOB37_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            complement(6611..6832)
                     /locus_tag="FOB37_RS00525"
                     /old_locus_tag="FOB37_00525"
     CDS             complement(6611..6832)
                     /locus_tag="FOB37_RS00525"
                     /old_locus_tag="FOB37_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          complement(3916..4392)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 accaccagaa acgccctgag gccgttttcg cggtgcgcgt aagaagggat tatggtaaat
       61 agggcgctat cgcctaaata cacggctgat tgtttttttt tctctgaagt atgagattcc
      121 tgagtagaca gctacaacaa gtagagcaat ggcgatagca agtgaaatcc agtccataaa
      181 tgcctgatta atttaataaa aacagcctgt tatgatttca taaagataat gtcatctttt
      241 tttagcatga tactgtcgtt acttattgtg gtgagatgaa acataaccgc tgtttttgta
      301 tgtgtttgtt aaggctgcta agattattcc taaccactga ttttgtacat atcatcaaaa
      361 aaataaaaaa actaatgccc ccgtggtggg tatataacaa atcacggtaa tggtgccaac
      421 ttactgattt agtgtacgat ggtgtttttg aggtgctccc gtggcttcca tctccatcag
      481 ttgtccttcc tgctcagcta ctgaaggcgt ggtgcgtaac ggtaaaagta ctgccggaca
      541 tcagcgctat ctctgctctc actgccgtaa aacatggcag ctacagttca cttacaccgc
      601 ctctcagccc ggtacacacc agaaaattat tgatatggcc atgaatggcg tcggatgtcg
      661 cgccagtgca cgcattatgg gcgttggcct caacacgatt ttacgacact taaaaaactc
      721 aggccgcagt cggtaaactc acgcatacaa ccgggcagtg acgtcattgt ttgcgcggaa
      781 atggacgaac agtggggtta cgtcggcgct aaatcacgcc agcgctggtt gttttacgcg
      841 tatgacagga tacggaggac ggttgtggcg cacgtgttcg gtgaacgcac tctggccaca
      901 ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatggat gacggatggc
      961 tggccgctgt atgaatcacg cctgaaggga gaactgcacg ttatcagcaa gcgatatacg
     1021 cagcgcattg agcgacataa cctgaatctg aggcagtatc tggcaaggct gggcaggaag
     1081 tcactgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
     1141 ataaaacact atcagtaagt tgaagtcatc accacaagta attagtgata catccagttt
     1201 tttccagtaa aatacctcaa taacccattt cagtatgtaa attcatgaat aaaagtatcg
     1261 agtcactaca agttattcga gccatagcag cctgttcagt agttcttaca cacatttatg
     1321 gcgcaagcgt acctgtaatt ggctcttcgg gtatctttgg tgttgatctg ttttttgtgc
     1381 taagcggtta tttaatgact attacatacc gcaaaaacag aacagggtat caatttataa
     1441 aaagcagaat attaagaata tatccacctt accttgtgtt atcactgcct cttattattt
     1501 tttcatattt ctataaaaac ataagtgcat ctgatatttt tcataacata accctcctcc
     1561 caactattca tggtaactat gataggataa actttgtttg ctggacatta acgtatgaat
     1621 tatattttta catagtattt tcattgtgca tgtacatatt caaaggggca aggaatgtta
     1681 tcttatccac ttcattcacc atgcttgttt tatttttttt agcgggtgga gttaacggcg
     1741 gattagcggg ctggaaagat aattctattc atcacatgat tggtaactat ctaatattag
     1801 acttcatact tggctcctta attggattgg cgtacaatgg gtacacatac aaaaaaaatg
     1861 cattccctat attgctatca atagcaataa taatcacgag ttgcaccatt ataggccaca
     1921 ttcacttacc tgagccatat aataactacc aactcattac atttctaaca tctgggattc
     1981 cagcatgcgt agtcatatac ataacatcaa aggtaagtgt agataaaata cctctaaaga
     2041 aagcattgat ttatatagga acagcttcat actccattta tctaacacac ctttacgctg
     2101 tgtttgcatt ttctaaacta actcataaca acgcacctga aaagtttcta tgttttgtta
     2161 cttcgatagt aataggctgc gttgcatatt acacaataga aaggaagata ataataaaaa
     2221 aaaagaacca tgtttgataa catgagcatt taataattgg ttaatgaacc tactgcgagc
     2281 ccatcccagt aggcgtaaat ttttacagcc agtttagaca gcgacagcgc gaaaaaaccg
     2341 gggcatacac ggagtgacct gctccccgtt ggttaataca ccgtgatgtt agtaatgtct
     2401 tcataagcca catgaggaca tcctcatgaa gaagcgtttt tccgacgaac agatcatcag
     2461 tattctccgc gaggccgaag ccggggtttc tgcccgtgag ctctgccgta agcacgccat
     2521 ttccgacgcc atcttttaca cctggcgtaa gaagtatggc ggtatggagg tgcccgaggt
     2581 taagcgcctt aagtcgcttg aggaagagaa tgccagactc aagaaactgc ttgccgaagc
     2641 catgctggat aaggaggcac ttcaggtggc tctggggcga aagtactgac gacagaccag
     2701 aaacgggaag ccgttgagtt tatgtgtgat gcgaccggtc tgtcgcaacg tcgtgcctgc
     2761 aggcttacag gtttgtccct gtcgacctgc cgctatgagg ctcagcgtcc cgcggctgat
     2821 gcgcatttat cagggcacat cactgagctg gcactggagc gcaggcgttt tggctaccga
     2881 cgcatctggc agttactgcg ccgtgaaggc cttcatgtta atcacaagcg cgtgtaccgt
     2941 ctttaccatc ttaacgggct gggtgtaaaa cgcagacgac gtcgtaaagg tctggcaaca
     3001 gaacgtctgc cgctgctccg cccggaggcg cccaacctga cctggccgat ggattttgtc
     3061 atggacgcgc tggccaccgg tcgcaggatc aagtgcctga cctgcgtgga cgacttcacg
     3121 aaggagtgtc tgacaattac cgccgccttt gggatttcag gcgttcaggt cacgcgaatg
     3181 ctggacagca ttgcactgtt tcgcggctat ccggcgacga taagaactga ccagggggcg
     3241 gagtttacct gcagagcact cgaccagtgg gcttatgagc atggggtgga gctgcggctt
     3301 atccagccgg gcaggccaac acagaacgga tttattgaaa gttttaacgg acgattcagg
     3361 gatgagtgcc tcaatgagca ctggttccgc gatatcgttc acgcgaggaa aacgattaat
     3421 gactggccgc aggattagag cgagtgtcgt ccacattcat cgctgaacta ccagactccg
     3481 gctgaatttg cggcgggctg gcgaaacggg aaatatgaag aaaaacgaac cgacattact
     3541 aactgaaggt tgtatctaat cctgggggca ggtcaaccgc gttggatgat tatggatcat
     3601 gattatatat gtttttttct tatcaaaaac aacgagttaa atagttttat tttgatggtt
     3661 ggtgtcgcgg agtggtgctg gtttcacttc aggggtaggc atatggaaca ggatcctctt
     3721 gagatccttt ctttagcatc gtaatctctt gctcagtaaa cgaaaaaacc gccttgcagg
     3781 gcggtttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
     3841 ggagcacagt caccaaaact gttcgtttag tttagcctta accggcgcat aacttcaaga
     3901 ctatctcctc taaatcagtt accagtggct gctgccagtg gcgcttttgc atgtcttacc
     3961 gggttggact caagacgata gttaccggat aaggcgcagc agtcggactg aacggggggt
     4021 tcgtgcatac agtccagctt ggagcgaact gccttcccgg aactgagtgt caggcgtgga
     4081 atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     4141 agagcgcacg agggagccac cagggggaaa cgcctggtat ctttaaagtc ctgtcgggtt
     4201 tcgccaccgc tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
     4261 gaaaaacggc aacgccgtgg ccttttccac aggctcttct cctcctggtg atagcgatga
     4321 ctcttctgtg tgtcccgtcc gctcgccgca gccgaacggc aggagcgtag cgactgagtg
     4381 agcgaggaag cggaaaagag aacatcccta cactgagcac tgacgccctg aggcccctta
     4441 tgacgaaaca acagaaaact gccctgaaca tggctaaatt catccagaat cagtccctgc
     4501 tcctgctgga gaagcttaac gagcttgatc tcgatgcggg ggctgacctt tgtgagaaac
     4561 tccacgatga tgcggagcat cttttccgta cactgtccct ccgactggat gcactacagg
     4621 agggtgactg atggcgaacg tgtcgatcag tgaggcagca cgcctgacgg gtaagagcag
     4681 gacaaccctg cacaggttaa ttaaagccgg tgaactgtcc acctgtcggg gtgaacggaa
     4741 tgcgaaaatg ctcgatactt cagagctttt gcggtttttt ggtgcctttg cgcccgtatc
     4801 cgttgaacag gaggaagaac aggtcagtgg acagcgtgac acagtcactt ctgaccataa
     4861 tgaacaggcc atttacattc tgaaacagga aattgaacac ctgaacaccc ttcttgcctc
     4921 aaaagattca catatcgaca gcctgaaaca ggcgatgttg ttgctggaga accgggaaga
     4981 gaagcggcag aacacgaacc gcccctggtg gcgtttctgg cagtcctgaa atgcctcagt
     5041 gtttttgccc tgctgttccg tcagtggtct ggagtttttc cagtttccgt ctgcgccgtt
     5101 ccgccgtcgc gcaggcattt ctccgtcttt cctctgcttt ctctgcttcc agacgggcca
     5161 gcacggtgtt atgccgctcc ccggccacat atttccgggc cttctccagg gttgtttcca
     5221 gcgccagctc ctttttctgc aggatggatt cgcgctgatt cagtgcctcc ctctgttctt
     5281 ccagctcctg cttggcttct ttccgggcat tctgcaccgc ctgaagcaag aggctttctt
     5341 tttgtgcaaa cgcttcccgc agtacatcct cagcaccact gtagtggctt cgcagggccg
     5401 ctgtctccag gttgtgattc tgaataaggg tattttgtct ggctctgtga gcctcttctg
     5461 cctccctcag agcctgctta tacctgtctg tctcgctctg tatccggcgg gactctgagc
     5521 ctctgaaaaa caaaatgaac agccacagcg agaacgctgt ggctgcgatc cccgtagcca
     5581 ggattgcggg accgagttcg gtgaccgcct tccagccagt gaacagctgc ggattgctgg
     5641 catccggata gtaaacgccc atatgtttca gctcatacag ggcgtacgcg atggcgaaaa
     5701 caccggtaca caataaaata aacgttcttg tcatcgcagg tatgtctccg tgatttccgg
     5761 acgaaaatgg cccatttcct ggctcacggt acgcagggcc atatcacgaa ccagaccgag
     5821 tttttgcagc tcctgcatcc gttgctgggc gtagctgtga cgcacgccgt gtgccccccg
     5881 tgaccagccc agggtacgca tggaggcggc actgaaggcg tttgaccagc gctggcctcc
     5941 gccaatggcg tactgactct ggtaaaacac gccgcgatcg gtcacccgga ccgggctggc
     6001 cagcctgaca tgctcgagac gttcagccag gcgtgacggc agcagaactt ccctgaccag
     6061 gccgcctttg ccctgaacgg tgtaaatcac accctcccga ccggcaaatt tactgtccgc
     6121 tgccggacgg ggatccggtg cacgctcctg gtggggcgcg agggtcagca gttcatgggc
     6181 gcgtaatccg gcggccaggg cgatcgcggt ggccagggcg ttggcgggct gttgtgctgc
     6241 ggcaatcagg gccacctggt ccgggctgta ggcccggctg ctcaggatct gtgcctgctc
     6301 cgatttcacc accggcaggt gttcgccggc agggagggtc tgcgtcacgt ggatcatcat
     6361 ggcctggatg gcctggcgtt ccatatcgag cgttttctgg ccgacctgct ccccgcgctg
     6421 ctccaggtag gtgatggcct gcgccggtgt cagtgtgcgt aaatcaccgt ggttgatgcg
     6481 gttaaatttc atccactctg cgacgcgggt cagtccctgc tcgtaattcc ggaccgtacc
     6541 gacagagcgc agaatatttc cctggagtgg ttttatcacg ccagccgcct ggcgggcagc
     6601 gctggcgaat ttagccattt ttttccagcc agcgggtaac cgtggacaac acgactttga
     6661 ttctccgggc ccggagccag cgttgcagct ccgctgcact ggccccggcc tggtacaggc
     6721 tcagcagttc gccggtatag cggtcaaggc gggagggacg gtagcggacc tggcggatac
     6781 ggcgggtctg cgcctgcagg gcggccagtt cggcggcggt atcaaattcc acaggaactc
     6841 cggtgttgag ttgtaagaac gccagtggaa atccactgcg gcctgcgccg atcgccctcg
     6901 tttaaaccgt aacgtggtta agcccggact gctcgcggct gtaaaaacgt cccttaagcc
     6961 agggaatggc ctgacctgat tgccggtctc gcagattttg gctgttttct caggattctc
     7021 ggattgggct gtccggtaca gtatccccgg ggggacccgg ctgttttacc tgctgccggt
     7081 aggcaggtgc gcgtcagagt gcgcctcctc atggtttgac taaatactag ccaggttttt
     7141 cgtgcgtgtg aatgttgttt tttaaatgtt gcgcctgact cagtttaata gcgacacccg
     7201 ctatgtacag gcgcatttta tgcgtcgtac atagcgggtg tcgcaggtgc tgatttggcg
     7261 gaataaaaaa caccgggact gaccgccccg gaccccgcca acggtgaccg gacagacaaa
     7321 aaatttgcct gcccggtcac cgtttcacat catggcgagg aggcgggatc tgaaacggtg
     7381 tgatgaaagt gcagatgagg tcagacgatg caggtcggtg agtccgtggt gaacgcgata
     7441 gtggaatacc acgcacaggg cagtgaggag gcgcatgcag aggaaaacag gatgttcctg
     7501 cgttatctgc gcgagttaac gggaacacag cgtgtggcgg ttgaggtggc caggaaatat
     7561 ttttctccgg tgaggggctg atatgagcta tctttttctt gtcgccggca ccgcaccagg
     7621 tgcgacggtc cttttacaga gttgtcctca tttgacgggc ctggatggcc cgttctttta
     7681 tgcttttaac gtccagaacc cttttctcgg ccgaaatcac cggctatgcg gtggtgtgca
     7741 tagccatgca tatgcgtaaa ataaatcagg cgcgtttctg gcggttttcg gtgtacttgc
     7801 caccaccttt tgcccgtta
//
