LOCUS       NZ_CP044040             3223 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_630 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP044040
VERSION     NZ_CP044040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11056345
            Assembly: GCF_008693405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Bachman,M., Young,C., Tallon,L., Sadzewicz,L., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Nandy,P., Geyer,C., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2019) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Coverage                   :: 695.31x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:59:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,547
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 59 of 140
            Pseudo Genes (incomplete)         :: 86 of 140
            Pseudo Genes (internal stop)      :: 22 of 140
            Pseudo Genes (multiple problems)  :: 24 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP044040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_630"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_630"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MI"
                     /collected_by="University of Michigan Health System"
     gene            300..785
                     /locus_tag="FOB38_RS27955"
                     /old_locus_tag="FOB38_27955"
     CDS             300..785
                     /locus_tag="FOB38_RS27955"
                     /old_locus_tag="FOB38_27955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            789..1004
                     /locus_tag="FOB38_RS27960"
                     /old_locus_tag="FOB38_27960"
     CDS             789..1004
                     /locus_tag="FOB38_RS27960"
                     /old_locus_tag="FOB38_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1172..1504
                     /locus_tag="FOB38_RS27965"
                     /old_locus_tag="FOB38_27965"
     CDS             1172..1504
                     /locus_tag="FOB38_RS27965"
                     /old_locus_tag="FOB38_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            2522..2845
                     /locus_tag="FOB38_RS27985"
                     /old_locus_tag="FOB38_27985"
     CDS             2522..2845
                     /locus_tag="FOB38_RS27985"
                     /old_locus_tag="FOB38_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2235..2426
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1772..2252)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          3..1102
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.244"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2836..3221
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.899"
                     /coverage="99.0"
                     /evalue="1.91e-75"
ORIGIN
        1 gcgcatcgat gcctggcaga ccatagtgaa cggcaggctg gggctgcatg acccgaacgc
       61 gccggagaac cggcgggtgc tggtctcgcc ttccgcgctg ccggaagcga agcaggaagc
      121 cgcccaggcg attacgagcg gcctgcttgc ccttgcctca tccggggagc tgaaaacgcg
      181 tcaggacgtc actgaggcgc tggaaagcgc aggttttgag gtggtgcgca ccacaaaaag
      241 cagcatcagc attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccatcta
      301 tgaacagtct tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga
      361 ctaccggcga gatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac
      421 tgagcgaaag cgagaagaga atcagcgccg ccatcctcga ccacgaccgg aagctgtcct
      481 cagccatgag ccagcgcacg aaagggatgc tgcgcatggt cagccagacg tggctgacca
      541 tcgttctggt ctccgcgctg ctgatagcgt cgagcgcggg cattctgtgg tggcaggggc
      601 agcagatact cgagaactat acgaccatcc gggagcagaa gagcacgcag gccatgctgt
      661 cagagaggaa cagcggcgta cagctctcga cctgcggcga gcagagacgc cgctgcgtga
      721 gggtgaaccc ggaagcggga cagttcggag aggactcgag ctggatgata ctggcgggga
      781 aatagcacat gacggagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg
      841 actactcgaa aaggctggac gagtgggaga acgccttcgc ggaatggcgg acgatgtctg
      901 gtcttattca acgggagaac gcggcgctga acgagcgcgt cacggtcttg agcaggcagg
      961 tgcagagttt gagcgagcag ctgcgccggt tgtcgaaagg ctgaacgcga tagaaactca
     1021 ccggcagcag gagcgggcgg tgcagcacca gaaagcgctg gagctggaac gatcgcagtg
     1081 gcagcatcat ggtccttcgc tatgataagc cgggttctat taattagttg ttatgtaaag
     1141 atatttaaat ttttcaacat gaggtatttt tatgaataca tatattttcc tgggaggggc
     1201 tattgtggct gaagtcatag gcaccacctt tatgaaatat tcagaaggat ttacacgcct
     1261 gtggccgtct gtaggaactg caataagtta ttgcgctgcc ttttatctgc tttcccaaac
     1321 attggaaaat atacctaccg gtattgctta cgcaatttgg tctggtgcgg gaattgtact
     1381 aataagccta gctggttggt tggttagcgg tcaaaaactt gattggcccg caattgcagg
     1441 aatgttcctt atttgcgttg gagttttggt tattaatatt ttttcaaaat cttctgcgca
     1501 ttaacttttt accgaagcat attggccaaa gcaaaaggat ttccttgaga tcctttcttt
     1561 ctgcgcgtaa tctttttccc tgtaaacaaa aaaaccaccg ctaccaacgg tggttcgttt
     1621 gccggaattc agagctacca actctttgtt ccgatggtaa ctggctttca cggagcgcag
     1681 atacctaaac tgtcctttca gtgtagccgc actcaggctt tcacttcaag aactctgtag
     1741 gcattctcta caatctctgc tccgtgaacc cagttaccaa tggctaccgc cactggcgat
     1801 ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac cggaacgggc gtggtagtcg
     1861 gactgaacgg ggggttcgtg catacagtcc atcctggagc gaactgccta cccggaaccg
     1921 agtttcaggc gtggaatgag aaaccgcggc cataacagcg gaatgacacc ggtaaaccga
     1981 aaggcaggaa tgcgggggag cacgagggag ccaccagggg gaaacgcctg gtatctttaa
     2041 gccgcatcgg gtttcgccac cgctgatttg agcgtcattt tcgtgatact cgtcaggggg
     2101 gcggagccta tggaaaaacg gcaactccgc atccttttct tggcctttcc tgcttcgagc
     2161 ggcatttgct tatcccgtaa tctgtgagct ggtaacgccc gccgcagtct cacggcagga
     2221 gcgtagcgac cgagtgagcg aggaggcgtc atttcatcac cacctgcatt ttgcacttac
     2281 gcccggcagc cgcatttttc ccctgtcaca tgaagcactt tcataaattg ccactccgtc
     2341 ataacatgtt tagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     2401 ccgaaacccg ctaaaaccac tgccgcgcct gcggcttgtc caacaccgtg ccgaccggga
     2461 aaagatttcc cgtccgtccc ggcgttatca ggaggccgga ttggcagaca aacgcagcaa
     2521 aatgctcacc atgtgggtga ctgaggacga gcaccggcgt ctgctggagc gctgcgaggg
     2581 taagcaactc gcggcctgga tgcggcagac gtgcctggac gagaagcccg cacgcgccgg
     2641 caaacttccg tcgatctcgc cggcgctgct tcgtcagctt gccggcatgg ggaacaacct
     2701 taaccagatt gcccgccagg ttaacgccgg tggtggaagc ggacacgacc gcgtgcagat
     2761 tgtcgccgcg ctgatggcca tcgatgccgg actcgagcgg ttgcggcatg ccgtactgga
     2821 aaagggtgct gatgatgatc gttaagtttc atcctcgggg gcgcggcggc ggcggcgggc
     2881 cggtcgatta tctgctgggt aaagatcgcc agcgcgacgg cgccagcgtt ctgcagggga
     2941 agccggatga agtccgggag cttatcgacg cctcgcccta cgccaaaaag tacacttccg
     3001 gcgttctgtc ttttgccgaa caggatttac cgcccggcca gcgcgaaaag ctgatggcga
     3061 gtttcgagcg ggttctgatg cccggactcg ataaagacca gtacagcgtg ctgtgggttg
     3121 agcaccggga caaggggcgg ctggagctga acttcctgat cccgaacacg gagcttctga
     3181 ccggcaagcg gctacagccg tattacgacc gcgccgaccg tcc
//
