LOCUS       NZ_CP043584            10060 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SCKP020018 plasmid p1_020018, complete
            sequence.
ACCESSION   NZ_CP043584
VERSION     NZ_CP043584.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08624691
            Assembly: GCF_003037185.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain SCKP020018
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Lin,J., Tang,G., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003037185.2-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:08:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,732
            CDSs (total)                      :: 5,608
            Genes (coding)                    :: 5,435
            CDSs (with protein)               :: 5,435
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 75 of 173
            Pseudo Genes (incomplete)         :: 101 of 173
            Pseudo Genes (internal stop)      :: 39 of 173
            Pseudo Genes (multiple problems)  :: 39 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043584.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCKP020018"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_020018"
                     /geo_loc_name="China: Meishan, Sichuan"
                     /collection_date="2016-10-15"
     gene            243..488
                     /locus_tag="C6M74_RS28515"
                     /old_locus_tag="C6M74_28950"
     CDS             243..488
                     /locus_tag="C6M74_RS28515"
                     /old_locus_tag="C6M74_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            762..1133
                     /gene="tolA"
                     /locus_tag="C6M74_RS28520"
                     /old_locus_tag="C6M74_28955"
     CDS             762..1133
                     /gene="tolA"
                     /locus_tag="C6M74_RS28520"
                     /old_locus_tag="C6M74_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1130..1495
                     /locus_tag="C6M74_RS28525"
     CDS             1130..1495
                     /locus_tag="C6M74_RS28525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1670..1948
                     /locus_tag="C6M74_RS28530"
                     /old_locus_tag="C6M74_28965"
     CDS             1670..1948
                     /locus_tag="C6M74_RS28530"
                     /old_locus_tag="C6M74_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1936..2250
                     /locus_tag="C6M74_RS28535"
                     /old_locus_tag="C6M74_28970"
     CDS             1936..2250
                     /locus_tag="C6M74_RS28535"
                     /old_locus_tag="C6M74_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2414..2842
                     /locus_tag="C6M74_RS28540"
                     /old_locus_tag="C6M74_28975"
     CDS             2414..2842
                     /locus_tag="C6M74_RS28540"
                     /old_locus_tag="C6M74_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2868..3047
                     /locus_tag="C6M74_RS28545"
                     /old_locus_tag="C6M74_28980"
     CDS             2868..3047
                     /locus_tag="C6M74_RS28545"
                     /old_locus_tag="C6M74_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3074..3604)
                     /locus_tag="C6M74_RS28550"
                     /old_locus_tag="C6M74_28985"
     CDS             complement(3074..3604)
                     /locus_tag="C6M74_RS28550"
                     /old_locus_tag="C6M74_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3611..4342)
                     /gene="mobC"
                     /locus_tag="C6M74_RS28555"
                     /old_locus_tag="C6M74_28990"
     CDS             complement(3611..4342)
                     /gene="mobC"
                     /locus_tag="C6M74_RS28555"
                     /old_locus_tag="C6M74_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4342..6306)
                     /locus_tag="C6M74_RS28560"
                     /old_locus_tag="C6M74_28995"
     CDS             complement(4342..6306)
                     /locus_tag="C6M74_RS28560"
                     /old_locus_tag="C6M74_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7790..7939)
                     /locus_tag="C6M74_RS28565"
                     /old_locus_tag="C6M74_29005"
     CDS             complement(7790..7939)
                     /locus_tag="C6M74_RS28565"
                     /old_locus_tag="C6M74_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8024..8281)
                     /locus_tag="C6M74_RS28570"
                     /old_locus_tag="C6M74_29010"
     CDS             complement(8024..8281)
                     /locus_tag="C6M74_RS28570"
                     /old_locus_tag="C6M74_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8291..9976)
                     /locus_tag="C6M74_RS28575"
                     /old_locus_tag="C6M74_29015"
     CDS             complement(8291..9976)
                     /locus_tag="C6M74_RS28575"
                     /old_locus_tag="C6M74_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3291..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7109..7687
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3615..4342)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cccgttttca ggtgtcaata tatgagatta gttctcatct tcaggcacaa aaaagcgccc
       61 ggagccacct gtaagcagat cgcgtctttt cccgaaaatt tctcatggca acaaatttta
      121 ttatttaaga cattcatcac acttacattt cagatggtat agacacatct gaaagctaac
      181 tggcatgagg agaggacctg cggttaaact gttcgggaaa cctgagacgc ccggaggcca
      241 ctatgacgca cctgaaactg actcccgctg aagaaaagcg cctggcggac gcctttgtcg
      301 ctcaggcact ggccatgggc ctgaaaggga acaaaacgct gccaaaggcg gtaaaacaac
      361 aggtgcggga aagggtgctt cgtgccattc aggacgaacg tgaagccagg cgggcgcagg
      421 aaatgcgtcg ggagaaatac gaggctacgg aaaccacctt cacctggcag ccacagcgcc
      481 gccggtaagg ggtatttacc ataacgtcgc attgcccgca ccgctcagac ggtctgaggg
      541 cgtttccggc ggctacggga taaaacaggc aacaaaccac ccggaaaacc gccggaaacg
      601 cgcccggtaa attgtaaccc gctgcatgcc tatgcacgca actgcatggc gggccgtttg
      661 gtgtaaaaac gatacgataa gccgtgttta tgtaaatgaa cgtggccgta aagagtgaca
      721 aatatacatg taacattttt tactgacagg tcatcaaccc gatgcgttta atacccttta
      781 tcctgctggc attgtgcagc cccttactcc tgacggcctg taagcattcc accacccagc
      841 cgccgaaaat ttatactctg gataagtcat ctaaaatcag tagctacgct gcggccatca
      901 agaccgctgt tgaaatgcag atgtttgaac cagaggccta tgcgggaaaa atgtgcaccg
      961 tgcggatatc catacaacgt gatggcactc tgaacagcgc cattgctgaa ggaggagatc
     1021 cgaaactctg taaagcagca atatcagccg ttacccacgc caaaattccg ccggcaccgg
     1081 atgaaaatac ctggcaaata ttcaaaaacg cccctctgga tttcagacca tgaaagcttt
     1141 tactctgaca gtgctggcgg ccatgttcct cactccggta catgcgatac cactaaaacc
     1201 cgtaaccaca ctgcagccaa cctacccggt acaggccgca gcaaaacgca tcgaaggtca
     1261 ggtgaccgcc acgtttgata ttctggacga tggacgagtg gacaacgtgt cgcttaccgg
     1321 ctgtcacctt ttttttcggg aaacgctgac ggcaatgagg cactggctat atcccgcagg
     1381 gcacccggca atgcgacagg ttataaattt tgaattcagt ctgaaaaatg acggcttaca
     1441 ggatccgcga ccagtcacca cccaaaagct caatgatgat ggttgccgcc agtaacaggt
     1501 cctttaaccc gcaggctgca atagtcctgg atgatgagtg agcctgtagc agccctgatt
     1561 agtggaagcg aagctgaaaa atgggcagca aaagccgtat ttctggcaaa tagcgccgcc
     1621 acggattaaa gtagtcatgt gtaatacata tgactacaaa ggggctttta tgtcatcagc
     1681 aacatccatc aggctcgatg aagaacttaa ggaccggctg aaaacgctgg ccgatgaccg
     1741 gcaccgctca gcgcacgcgc taatgctgga ggcaatcacc gaatacatcg accgggaaga
     1801 gaagcgcagc cagtacctgc gggacggaca ggccgcatgg cagcactatc aggaaacggg
     1861 tctgcacctt actgcggaag aagcggaagc ctggataagt acctggggaa ctgagaacga
     1921 gcaggatgct ccgccatgcc acaggtaacg atttccgcgc tggcacagcg cgatttacaa
     1981 cgccttcagg actttctcaa aaccaaaaat cggctggcag ccagaaaagg aggtgaggtg
     2041 atcgtccggg ctatccagca actgaaaaca ttgccagaca ttggccgccc ggtgccgttt
     2101 ctgccgctgg aatataagga actggtgatc gggtttggcg acagtggcta tgtgatgctc
     2161 taccgccacg accgggaaat ggaccagatt gtgatcgtca ccgtcaggca tcagaaagaa
     2221 tccgggtatc cgggggcaga cagcctctaa taaccgatga cgggggcgca attcattgcg
     2281 tggccgtcgc cggtttgaca agcgcagcgc gtcagaaaac cacatcctga acggaaaaac
     2341 gggtaaaatt cagcgataca tttaccgtga gaaattacac acctttcaca cccgataaaa
     2401 tcaggggtat acagtggcga aaacattgct caacctttca caggcagcac aagccgcggg
     2461 cattaccaga agaacgctat acaatcatgt aaaacaggga aaggttaccg tgtcacgtga
     2521 cgggaaaaac aaccctgtcg tggatgtgtc agaactgatc cgtgtatacg gaaatgtgaa
     2581 tatccctgag aaacaaattc ccggcatttc acaccgtgaa aatacacaaa aaaatttccc
     2641 acaggaacag cttctggcaa tgcaaaagga actggccgat ctccggcagg ctgtcaccct
     2701 gatgctggaa gataaaacat cccgggaaga agaacgccgc cagcacgatg atgagcgccg
     2761 gaaactccag gccgaagtgg acagattgac aacagaactg acacagaaga aaaaacgctt
     2821 ctggtccggc tggtttagct gagaacttac cgaagggaat ggagaacatg acgaagcagg
     2881 aaaaaactgc cctcaatatg gctaaattca ttaaggatca gtcgctgctg ttgctggaaa
     2941 agctgaacga gctggacatg gataccccgg ccgacatgtg cgaacggctt cacgaggatg
     3001 cggaaacact ctggcaggtg atgcgggata agctcgggga agaataacag caccggccat
     3061 gccggtgctg gcgttatttt ttgccgaacg gccaccaccg gcgggcgggt ttgtcctccg
     3121 gcagttcagg caccggcacg ttaaaatagc ggctgttata ttcccgcatc agctccgctt
     3181 tcagcttctc gttcaccgcg ctttccccgt gccgctcgct gaccacctca atcatcgcgt
     3241 taatctgctc cggtgagcac ttcccggcat caaccacctg cacaccgttc tcgatggcga
     3301 accgctccac ctttcgatag aggtaatcac acagcttcat tcctgtgggt ttgtcccgtt
     3361 cccattgcgg attttcaccg gtgaaaaagt acgtggcttt ttcgtcgctg ttgagccgct
     3421 cgtcatacat cccgatggat ttcatcatcg ccagatgctg ggcccggtcg acaaaacaga
     3481 aaacatacgc cccggtctgc caggcgagca ccaccatgtc ttccagatgc tcgctggttg
     3541 gcataatgca gcgctcgccg tctttgtctt ccacgtcctc aataaatacc atgtacagcg
     3601 ccataatccc tcactccgtt gtcataaatt taaagcacgc gaacagcctc tcccggtcca
     3661 gctccaccgt tttgcgggcc tcgccggtct gtggattcac gatttcatcc accgccagaa
     3721 tcgcccgctg tacccggaca agcgtcggcc ggtcagggca gatgtaccag atttcatcga
     3781 tgccgttcgc ccggcggttg aacagatacc cggacaccac ttcctgatac cgcctgcggc
     3841 tcttgacggt gcgctctacc tcaaatgcca ccgttctgcc gtccagctcc gccagcgcgt
     3901 ccggcacttt catccctttc agggccatac ggtgcaggta ccgccagccg gtggcccccc
     3961 ggccctccat ggagaggcgc agggactgca cggccagccg gtgcggcagc tgcgccgccg
     4021 acacccttga cggctgaaac ggaataacgt ccgtcagcgg ctcgtcctcg tcgaacgaga
     4081 acgccacccc gtgcggcgtc agcccccaca cgctgacctg acggccgtca accgggtatt
     4141 tcgcccggac cagcagccct ttcgccacca gactgtccag gaagcggtaa accggctgta
     4201 ccgccgcaat ccccagccgt cgcgcaacca gctccgccgt ggaacacagc tcttcccgca
     4261 gccagcgcag ggtcagccgt gttttctccg cgttccgcgc ctgccgcttc gcgtggctga
     4321 cgttgtactg ctccagtgtc atcagtaaag ccccgcaaaa tcatcatccg cttttggtcc
     4381 gccgccgtcg gccggaggca ggtactccgc cagcgggtcc gttttcgcca gcaccgggaa
     4441 ggttttcagc gaaatttccc ggcgctcaac ccgcagcggg ctgctgaacg ccagccgcgc
     4501 cacgcccagc ccggtcatca ccgcgaaccc gtcaggcagg tgttgcaggg tgttcacgtc
     4561 aaacagcggc cgcgcctcct tctggatgaa gctgtcgccg ctgatgtgct ccacgttccc
     4621 cgcctcccgg ctggcccggc ggcgctccac gtccacccgg atctccccgg tctggcccgc
     4681 cgcccactgc gcggactcct ggctggccgc gcgatagaac cagcggatcg gcgtgttatc
     4741 cagtaccgtg gttttcacaa aatcagcggg cagatcctgc ccgcactgac cgaagtcgcc
     4801 cagcgactgg tgcgcgagca gcaggttgca gtcccggctg cgcagcgtcc ccagcgcgtt
     4861 cagcacgtac ttcgacagca gatatttaat ctcatcgagc atgatgctcg cgtgcggcca
     4921 cctccggaac tcatcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcatcttctg
     4981 cacccggatc accgcctcat cgtccatcga gccgatgacg tacaggcagc cgccgccgtt
     5041 gattatcccc gccacatcga taccgctgtc cgtctgcagg acgctcaggt cggccacgtt
     5101 ctccagcttg gtgatcagcc ccttcacgtc ctttttcagg gcctccggca acgcgtacgc
     5161 cgccgccagc acctgctgaa tgtttgcccc ctgcgggaac tgctccgcaa tcagcttcgc
     5221 cgctttctgc tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa
     5281 cccggcaacc agcagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg
     5341 cgccggtttc cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac
     5401 gtgcggcgcc cacgcgtccc ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg
     5461 tacgcagcga atgagcgcgt tggtggccat cgcgctcttg ccgctcccgg gcaggccgag
     5521 gatctgaata ttggtcttgc gccaggtatc acgagggacc agcaccggct ggccctgctc
     5581 atccttaccc agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag
     5641 cgcgttaacc ggtaccggct ccccgaaatg caggtggcgg acgtctgtca tttctccggt
     5701 ccgctggctg ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc
     5761 cgcaaaatga ccatcaagca gcaggaacgc cagcagccgc gccagaagga cgccggcgac
     5821 aagccctccg gcacaccagg ctatcccgaa caggttagcc ggctgcagca ggtaataatc
     5881 tgcgttcgca tagaggtccg ggcgcaggac ggtttctccc cagctctcga taaacacggc
     5941 gacaaccagc ccccccagaa gggccagacc ggcagcgccg gtcagccagt acgcgtagtt
     6001 ccagacatgt cgccgtgccc tgaccatccg cccgggtgaa tccagcgagg ccagcaccga
     6061 aaatggcaca gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt
     6121 atggctcagc cccacaaaac gcattcggcc ggcccagagt tcagccagac cgaaaaacag
     6181 gccgataacc agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca
     6241 cgggtaaatc tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt
     6301 aaacatgcaa tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct
     6361 gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg
     6421 ttcagctttc accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg
     6481 gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt
     6541 ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat
     6601 caggagcagc tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc
     6661 aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag
     6721 agccagaaag aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct
     6781 tttcccctga agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa
     6841 aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat
     6901 cctgataatt gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag
     6961 cgcgtccggt gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg
     7021 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
     7081 ttccgtggat aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg
     7141 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca
     7201 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
     7261 ttaaagatcc ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct
     7321 gccgtttacc ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata
     7381 gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca
     7441 agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa
     7501 cccgggaaag cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     7561 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     7621 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     7681 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     7741 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     7801 cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccgccctga acatcccgga
     7861 tataatttgc ctgacatgcg gccagaagaa agccagcaac aatcaatata aaaagaaaaa
     7921 tagtctttac ctttttcata tactttccct gtatttaaag actccggcat tcatccctga
     7981 acaccggaag tcagtatttc aaatcatcct tgataatttt ttgttaccag ttaccgtccc
     8041 gataatcaaa agctatataa caattatcca catcaggttt tatttgattt ttaaaaaatg
     8101 gctgtagtaa aggaacccat tctttcttca aatcaaactc accattatta atattatcct
     8161 ttaaaggcat tcccaagctt tcaatgacag aaccatcgtc accaaagtct ttagaatact
     8221 cttccccttt aaactcttca gttttcttat caaaccatgt caaacgtaat ttaagcccca
     8281 tatttacctc tcaaagatat tttttaatgt tccgttttgg atctggccct ttaacctgct
     8341 tacccgtttt gggatcaaat gcgcctaggt gttcgccatc actggcacga tacccttcaa
     8401 gctcaccgtg ctgggaatcc cattcataaa ttttacgttt tttatcgcca taccatcggg
     8461 cccgcttacc accaccacct tgtttaggtg ttttaggatc acctcgcttt aactctccaa
     8521 gtccctttat gtcttctgtt ttaggtactg gatgataatc atggccgtag tctttagtcc
     8581 ccttgcgagg cttgttcttt tcctcattta acttattttc tgcatccttc gcttttttct
     8641 ccttttgttt ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt
     8701 cattcattgc gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat
     8761 ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat
     8821 aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa
     8881 cagcagccct tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
     8941 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
     9001 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
     9061 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
     9121 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
     9181 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
     9241 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
     9301 gacgttttgt cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg
     9361 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
     9421 cctggtccag tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg
     9481 tcataatttt tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt
     9541 agatcgcaat cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag
     9601 caacggcggc cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag
     9661 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
     9721 taccgctatg attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa
     9781 actcccccgg attatagtta tgaatatctc cattcggcgt atgcgtcgta ccccagcccg
     9841 gcccgctgtt tggatccgta ccaccacctg atgaagggcc acctttacca gacgtaccgt
     9901 tcacattccc actggcctga ccaccattat gtcccagacc tgaattaccc ggacctcgac
     9961 cgtctccacc actcataccg ttttcctctt tgacatttaa aacaataaat taaagccaac
    10021 atactgtaca tatacacagt ataattatta ccttactcct
//
