LOCUS       NZ_CP043464            10879 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Acinetobacter baumannii strain 18WIARLN0023 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP043464
VERSION     NZ_CP043464.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12287002
            Assembly: GCF_030585285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Florek,K.R.
  TITLE     Carbapenem Resistant Acinetobacter baumannii harboring the
            OXA-24/40-like resistance mechanism OXA-72 finished assemblies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Florek,K.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Comunicable Disease Division, Wisconsin
            State Laboratory of Hygiene, 2601 Agriculture Dr, Madison, WI 53718,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 460x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/02/2023 17:57:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 101
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 26 of 89
            Pseudo Genes (incomplete)         :: 59 of 89
            Pseudo Genes (internal stop)      :: 16 of 89
            Pseudo Genes (multiple problems)  :: 11 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043464.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="18WIARLN0023"
                     /isolation_source="Skin/Wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WI"
                     /lat_lon="43.78 N 88.78 W"
                     /collection_date="2018"
                     /collected_by="Wisconsin State Laboratory of Hygiene"
     gene            1..951
                     /gene="repM"
                     /locus_tag="FZ922_RS19745"
                     /old_locus_tag="FZ922_19930"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="FZ922_RS19745"
                     /old_locus_tag="FZ922_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="FZ922_RS19750"
                     /old_locus_tag="FZ922_19935"
     CDS             944..1519
                     /locus_tag="FZ922_RS19750"
                     /old_locus_tag="FZ922_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="FZ922_RS19755"
     CDS             1539..1682
                     /locus_tag="FZ922_RS19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1865..2692)
                     /locus_tag="FZ922_RS19760"
                     /old_locus_tag="FZ922_19940"
     CDS             complement(1865..2692)
                     /locus_tag="FZ922_RS19760"
                     /old_locus_tag="FZ922_19940"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            3301..3657
                     /locus_tag="FZ922_RS19765"
                     /old_locus_tag="FZ922_19945"
     CDS             3301..3657
                     /locus_tag="FZ922_RS19765"
                     /old_locus_tag="FZ922_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269902.1"
                     /translation="MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKGGKKRFYKEMLDIADQQ
                     YELHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3650..3952
                     /locus_tag="FZ922_RS19770"
                     /old_locus_tag="FZ922_19950"
     CDS             3650..3952
                     /locus_tag="FZ922_RS19770"
                     /old_locus_tag="FZ922_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3945..4154
                     /locus_tag="FZ922_RS19775"
                     /old_locus_tag="FZ922_19955"
     CDS             3945..4154
                     /locus_tag="FZ922_RS19775"
                     /old_locus_tag="FZ922_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(4480..4941)
                     /locus_tag="FZ922_RS19780"
                     /old_locus_tag="FZ922_19960"
     CDS             complement(4480..4941)
                     /locus_tag="FZ922_RS19780"
                     /old_locus_tag="FZ922_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(5246..7657)
                     /gene="znuD2"
                     /locus_tag="FZ922_RS19785"
                     /old_locus_tag="FZ922_19965"
     CDS             complement(5246..7657)
                     /gene="znuD2"
                     /locus_tag="FZ922_RS19785"
                     /old_locus_tag="FZ922_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(7785..8099)
                     /locus_tag="FZ922_RS19790"
                     /old_locus_tag="FZ922_19970"
     CDS             complement(7785..8099)
                     /locus_tag="FZ922_RS19790"
                     /old_locus_tag="FZ922_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(8086..8373)
                     /locus_tag="FZ922_RS19795"
                     /old_locus_tag="FZ922_19975"
     CDS             complement(8086..8373)
                     /locus_tag="FZ922_RS19795"
                     /old_locus_tag="FZ922_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            8682..9509
                     /locus_tag="FZ922_RS19800"
                     /old_locus_tag="FZ922_19980"
     CDS             8682..9509
                     /locus_tag="FZ922_RS19800"
                     /old_locus_tag="FZ922_19980"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(9553..9744)
                     /locus_tag="FZ922_RS19805"
                     /old_locus_tag="FZ922_19985"
     CDS             complement(9553..9744)
                     /locus_tag="FZ922_RS19805"
                     /old_locus_tag="FZ922_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     ARG             complement(1869..2692)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8683..9506
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa ggacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa cccgactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga
      661 ttttctttta cctttaaaca gaagaagaag gatagcccat caatagaaag agatccgaac
      721 actttggagc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaacag aaagctacca acagtttgct
      841 gtacgtattg ctgagatgct acaagatccc gctcaattca aagagctata cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggt gtattatgtt aattttagaa
     1861 attattaaat gattccaaga ttttctagcg acttataagt aatttcatta cgaatagaac
     1921 cagacattcc ttctttcatt tctaagttga gcgaaaaggg gatttttttt ccattagctt
     1981 gctccaccca accagtcaac caacctacct gtggagtaac atccattccc catccacttt
     2041 ttgcataaat cttactacca tttacttctt taattagaag cattttttta acttcttctt
     2101 gagtttctaa tttaaaaggt aatcggttat gtgcaaggtc atcggcaaaa ttaacttctt
     2161 gtactggtgt aattttaagg gggccaacta accaaaaatt atcgacctgt gttccaatat
     2221 ttgtatttcc aaaattaacc cgctttactt ctttctgcat tagctctagg ccagtccgtc
     2281 ttgcaagctc ttgatatact ggaactgctg acaatgccat tgcctcacct aaagtcatat
     2341 ctttctccca cataggataa gttctttttt taccatccca tttgaaaatc tcatttgttg
     2401 ttgctttatg attttctagc ccgattaaag catttagcat cttaaatgtt gatgcaggga
     2461 catattcttt atttgctcgt gcaagagcat taccataggt gctaagattt ttaccctctt
     2521 taataataat tacaccctgt gtttgagctt catcaaaata gcttttaata gctttttcat
     2581 gttgctgaga agaaatatga aaattatctt cagatttagt tttaatagat gaacatgcac
     2641 tgagagaaac tagaatagaa atgctgaata taggaagtat aaattttttc attacaaatt
     2701 catgttaggg gaaattttgg ggcttagagc ataatatttt tattaaaaaa gttaaaactc
     2761 ttttaattta acataatggg cgttatgcga aatcaattta aattatttta attaaatcaa
     2821 atatttatgt attctacata acgccaaaaa ataccagaat gccgactcgg aaacaagttg
     2881 ggtatttttt aagcttcttt gtcactttta gccaataaaa tagattaaaa aatattcaaa
     2941 aattcagttt tctttgattt ctcaacagtc agcattttga aataatccac aaaatttgcg
     3001 gataaatctt gggataaata tacgccaatt atgtacgcct aagcgcacac cgattgaggg
     3061 tattaaccaa tagttagact cagcttaatt ttctatcatg gacacataga gagcaggatg
     3121 ctccagcaca caaaacaact atcataagtg agcgcactag gatcgtgaag caaagtagaa
     3181 gttacacttg agatgcaaaa acgaaaaagc cagacaggat gtcgggcttt tttattgtca
     3241 tttgaataat aattcaatta agcataaaat atagactgta atctatatta tggttgtaat
     3301 atgtggacag tcattacaac ggatctgttt aacgaatggc ttgcacagca agatgaatct
     3361 acacaagaga aagtactcgc agcgttggtc gttctacagc aacagggacc aagtttaggt
     3421 cgtcctttag tagataccgt ctatgactcc aaatttacca atatgaaaga gctacgtgtt
     3481 cagcatcgag gtaaaccact gagagccttt ttcgcatttg atcctttacg acaagctatt
     3541 gtgctgtgta tcggtgataa aggcggtaaa aagcgttttt ataaagagat gttagatatt
     3601 gctgatcagc aatacgaact tcatctctca actttaggag atcaatctaa tggctaagac
     3661 cctacaagaa ttattggcta gtcgctctcc agagagtcaa gctcgtattc aacaaatggc
     3721 tgatgaatta ctgttggaaa ctcagcttca ccttattcgt gaagagttag agatttctca
     3781 aaaagaactg gctgcaaccc ttggaatcaa acagccgtca ttgtcagcaa tcgaaaatcg
     3841 tggtcatgat cttaaaattt caaccatgaa aaagtatgtt gaagcaatgg gcggaaaact
     3901 tcgtattgat gtagagcttc caacaggaaa acacatagga tttaatgtct aataataaac
     3961 ctttgaaaaa tagtactaaa ttacttgtta acttggacaa gttcatattt ttagtcaatg
     4021 ctgccgatag cctagaagaa attgaaatta ttagagattt atgctgtgac tatttttcaa
     4081 attgtaagag gtcaagctat tacattgata tttttgacga tgcttattgg ataaaatatt
     4141 acgaaatagc ttaatccttc cagattcgca taagagattt tatgttaaat gccgaactga
     4201 attaaaaata gaaatgtagt tgagctgatg cttgttcatt tctttatttt tagtaattga
     4261 ggtgaacata ctgtcagtac atggtagtta taagtaacgt gtaactttgt ttgcacaaag
     4321 atttgagtta tgccaagtga tggattgatt tgtacttaaa attttaagct tatccacttg
     4381 ctttgcaagc cactacactt tatttacaat cctatgttgt ggaagtaagg gatcttgtac
     4441 tcgcccagta tgttcaactt gattatatct aaaggactat taggcttcaa tcaaatcaat
     4501 tttccaatta gtcgactgaa ttaacacgtt taaatcacgt aatttaagac taatatcatc
     4561 agcttgtttt tgtaatgatg aaactggaat aactttatgc cactttattt cccgcgtact
     4621 ataacgatca gattcacgag atgtattact tattgctgca gttagggttt tgtgctgttc
     4681 tacaaattca tcacgcaaag tcaataaagc aagtagagaa cggccatcat ttaattttgt
     4741 taaagcatta gtttcatgaa ttgcataact taatttttga aattcttgaa ttaaagaaaa
     4801 tacttgtttt agtagttcat ttggatcttc agatggttga tcaccatctt gtaccaatac
     4861 atttgcattg atacgttgtt ttagtgaaat aatttttttt tgttgatcac ttcgaagtaa
     4921 gagagcttct gctaatttca tttttcacct attaaccaat tagaattaag ttttaaacag
     4981 cttatatcct cattctttaa agatcaaaaa aattgctttt tatctagcta aaaggtgtct
     5041 acaaaatcgg tggctattca gtgctgaaaa ggctgccgac acataaagtg acccagattg
     5101 aagagttact gccacactgc tggaaaccta aatcgaatta aaaaataggt atgggattca
     5161 gcggacgctt accttaatta ttaatctaat cattttataa gaaaaatctt ctaagaaatt
     5221 ttcttagaag attacagtat cttatctaga aattaagatt taagcctaaa ctaaaatttc
     5281 ttccaatttg gggaatatat ggcaaaaatg tttcatgggc atagactttc tgatcaagta
     5341 aattattcgc tttaaagaat aaatcatatt ctgtgtgaga tagcttattt ttatatgcta
     5401 aagtcatatt aagcatatta taacctgatg taacctgctc aaatttagaa atattatctt
     5461 gtttaaagac tcgataatat tctatttcac ctttaagcga ttcatcaaaa tcagctttta
     5521 gtcgtgtacc caaacgagca ggaggtaatc taggagtgta acgatccttt tggggagcta
     5581 aagtcggttc tctattccag atatcgtatg caattacagc atcaggtaaa tttgttaaac
     5641 ggcctttcac ataatctcca aaaagagagc cgtgataaac agaattaaat tgatacccta
     5701 tgttgccctc taatccataa aaattagcag cagactggct ataatgatta atacgtagtg
     5761 tatgctgatc tctaactttt gtagttccta aaacttcatt gagtgtctgt aaatatatat
     5821 agttatcaaa atcataatag taagtactga tttgataatc aagtaaatca tccttatagg
     5881 ccagactgat ttctaaatta ttggatttct cttttcttag aaagcggtta cctatttcaa
     5941 aagaattagt agcgagatgc ataccatgag tatacatttc ttgtgcatta ggtaatcttt
     6001 cttgatgtgt acctgttaat gataagatat aatttggagc aaatctccag tggactgtac
     6061 ctgcataaga aaaagctgtt tctttgttag gttgaacagc ttctaaaata gattttaaat
     6121 tttgagccct gattttatta tttttttcta cataaggact gttatattta ttaggccaag
     6181 gtttcattga atctttaatt ttttctatat cataatccat actaactttc tgcttttcta
     6241 tacgagcacc tagttcgaaa gtaaagtcgt tccagttata tttttctagg ccaaatacac
     6301 taaagttttt agtgacattt cgatttaata gctgctgatt atctagatag gtggtatggc
     6361 cttcttgaga atggactggt gataaagcac tattatcctg ttccaaatat tgtagcccca
     6421 agataccggt taattcccct aatggctgat gtgataactc tatgcggcct acttttcctg
     6481 tatttttaaa aaagttagta atcttatctc cctcgagttc attgtgaaaa taatctatat
     6541 aactcaatga ggtacgaatt ttgtcaatac cggtaaatgg ttgagtgaat tctccacgca
     6601 catcataacg tcgagtattt aaatcaatat aaggagcatt atgagagaaa ttagtttcat
     6661 gaatatgatt atgctgatga caatcaggac gtggatttat gtaattaata tcttgctcat
     6721 ccatcaattg aggataatat aaaagataat tttttaatcc actaattcgt gaatcgatac
     6781 cgattgcaga ggcaccacaa ccttcatata aatggctatg ggctggtaaa ccatattcat
     6841 catgacgatg tgtataagaa actcctaaat aagtattatc gtctatccag gataatccaa
     6901 gcgtacctac tctcgactct gcccagcttt ctggtaaata ttttaaattt ttataaatac
     6961 tgttttggtt ttcaagatct tctaaagaat tataagagct agactggtaa tgaggagttt
     7021 tatagttacc agcattacga tataaccctt ctgcccgtaa agctacatgt gggcttaagc
     7081 caactgttac acctgctgtg gtcaacttct cattactgcc agtattaaaa cgtaatgtag
     7141 tattaccctc taatccgtct tgaggcattt gggttggaat tttatagtca atgacattga
     7201 ctacccctgc cgcactattt gaactataca aaagggtact tgcaccacgt ataatttcaa
     7261 tagacttagc taaacttggt tcaactgtga cagcgtgatc tggggacata ttagacatat
     7321 ccaacacgtc agcattattc tgtaaaactt taatacgttt tccttcctgc ccacgaataa
     7381 taggagtaga tgctccacct ccatattgat tagaatgaat tcctaattcc gtacccaagg
     7441 catcccctag agttgtcgaa cgctgtttaa gtttactagt atcaataaac tgattactaa
     7501 catttagact tttgcctaac agctcagacc ctaactcatc agattgtgca gttaatacta
     7561 ttgtatttaa ggtagtaaca ggcccttcag ctgccaataa aattttcgag gcccctaaag
     7621 taattaaagc taaaaatagt ttacttttat ttagcataaa acaaccaaat ttaatgttat
     7681 attataatat aacacttaaa aatttataaa atctactatt taaatttttg aatggtaatc
     7741 ccccctaaaa atgaagtcaa aaatgggagc ctaagctccc attcttattg atgatttttt
     7801 aattctttta gcatagcatc tcgtaaaatt tcattcatac gtgtttgata acctttgcct
     7861 tgtgctttga accaagcaag tacatcagca tctaaccgaa ttgaagtttg ttgctttact
     7921 gggcggtaaa attgattttg gcgtacagca ttgctccagt ctgtaatttc aggaatatct
     7981 gatagatcta gctgatcatc aggaaccgtg cctttagcaa gcaagcgttg aatttcagca
     8041 tcttgcttct caccaaattt ctcattcagc tctttgtgtg agtatctaac catgctcata
     8101 tttgtttcgc tccgctttag tgacttgcct tgcactaatg attcggatga tttcacagtc
     8161 atcttcatca aaaatggtgt gagccaccaa taacattagt acgcctttaa ctcgtccaat
     8221 ggtttgccaa cgttcttcac cattggtatg tctgtcttgg attgagatcc gtaatggatc
     8281 ttcaaaaaca aggcttgcag tttcgaaaga gatatcatgc tttttctgat tctttcgatt
     8341 cttagcctca tcccattcaa aatactgttc cataaaaaac attcaaattt gtatatacaa
     8401 taatgtatca caaaattgta tcactcaacc agattcgtgt aatttttagt caaaattatt
     8461 ggcaagaagt atcaaaactg ctcataaaaa agccgacttg tttcaaagtc ggctttttgg
     8521 gcattttggg atttttctat ttttattaag tagttgatat ttatattaat atacaagtgt
     8581 acttcgtata atcgccatta tgttaaatta aaagagtttt aactttttta ataaaaatat
     8641 tatgctctaa gccccaaaat ttcccctaac atgaatttgt aatgaaaaaa tttatacttc
     8701 ctatattcag catttctatt ctagtttctc tcagtgcatg ttcatctatt aaaactaaat
     8761 ctgaagataa ttttcatatt tcttctcagc aacatgaaaa agctattaaa agctattttg
     8821 atgaagctca aacacagggt gtaattatta ttaaagaggg taaaaatctt agcacctatg
     8881 gtaatgctct tgcacgagca aataaagaat atgtccctgc atcaacattt aagatgctaa
     8941 atgctttaat cgggctagaa aatcataaag caacaacaaa tgagattttc aaatgggatg
     9001 gtaaaaaaag aacttatcct atgtgggaga aagatatgac tttaggtgag gcaatggcat
     9061 tgtcagcagt tccagtatat caagagcttg caagacggac tggcctagag ctaatgcaga
     9121 aagaagtaaa gcgggttaat tttggaaata caaatattgg aacacaggtc gataattttt
     9181 ggttagttgg cccccttaaa attacaccag tacaagaagt taattttgcc gatgaccttg
     9241 cacataaccg attacctttt aaattagaaa ctcaagaaga agttaaaaaa atgcttctaa
     9301 ttaaagaagt aaatggtagt aagatttatg caaaaagtgg atggggaatg gatgttactc
     9361 cacaggtagg ttggttgact ggttgggtgg agcaagctaa tggaaaaaaa atcccctttt
     9421 cgctcaactt agaaatgaaa gaaggaatgt ctggttctat tcgtaatgaa attacttata
     9481 agtcgctaga aaatcttgga atcatttaat aatttctaaa attaacataa tacaccttat
     9541 gcgaagccca gattaatcct gggctttttt ttcaatttgc ttattagctg taaacattcc
     9601 aggattagtg gttcggatgt tttcctgaca gctactacaa agcattaacc ctgcaaaaag
     9661 ttgtacattt tcctttgatt cacagaatcc acatgattta ggaaaaggaa cgatatttgc
     9721 taagttgact aattcttttg acataatgtt tatcctcaac ttgtacatat taatttcaat
     9781 atttttcagt atgttatacc taatttcacg ttaaactttg tacattcttg tataatccat
     9841 gtgtacaaac tcttttttat aaatttcata tatttaagcc taaaaaacca ttaatttatg
     9901 tacaacacta tgtacatact aaaaactata taaatcaaaa aattagtatt aaaaagatca
     9961 attattttgt acacaattca gaacctataa aaacaaccaa aaaccttatg aaataaagct
    10021 ttgaaggtca tcatctttag atgcgtggtg tgtgacaaaa aaatcccggc atgtgccgga
    10081 tcttggattg gaaaattggc taaagtgacg tagggccggt gcttggtttt gcatggaaaa
    10141 aagtatttat ttccttgttt agaaaaacgt aaaaaatcaa ttttttgtcc attcatccag
    10201 gttaaaaatt tcaacccaaa actttaatta tgaaaagctt catagaacgc gttcaaatgc
    10261 gatttaagag cctttaccta gaaaacatag atctcatagt gaaaaacaca aaacagctca
    10321 aaaaacgcaa atgagagcga aataaagagg tattttgact ttagatagat gcatagagcg
    10381 agtgtctacg agcgaactat caaaatttgc gcctagaccc tctgaaaaac atttttttgt
    10441 cctctttagc ctaagaaagc gtaagtttcg tgcagaaatt tgctcctgga tcgagcgtag
    10501 cgagtaaaaa agttttatga gcgaagcgaa ttccaagttt cttttgcttt tttttaaagt
    10561 cacgccagta ttaaccaaaa aattgcccca acgaactgag cgaaagcgaa gttcaataga
    10621 gtttgagcga agcgaaaacc aagggcaatt tttcattccc tgggctttta attattttaa
    10681 agtttttaaa tacttttaaa tggcttgaaa gccttatgaa taaagggttt tagctcttat
    10741 atgtccacgt ttaccttgca atatgtccac gtttaccttg caatatgtcc acgtttacct
    10801 tgcaatatgt ccacgtttac cttgcattag tacacaaata atattaacgt gtacttatac
    10861 acaataaaaa atagtggct
//
