LOCUS       NZ_CP043457            10879 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Acinetobacter baumannii strain 18WIARLN0025 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP043457
VERSION     NZ_CP043457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12287006
            Assembly: GCF_030585245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Florek,K.R.
  TITLE     Carbapenem Resistant Acinetobacter baumannii harboring the
            OXA-24/40-like resistance mechanism OXA-72 finished assemblies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Florek,K.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Comunicable Disease Division, Wisconsin
            State Laboratory of Hygiene, 2601 Agriculture Dr, Madison, WI 53718,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2019
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/02/2023 17:55:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,945
            CDSs (total)                      :: 3,851
            Genes (coding)                    :: 3,733
            CDSs (with protein)               :: 3,733
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 53 of 118
            Pseudo Genes (incomplete)         :: 60 of 118
            Pseudo Genes (internal stop)      :: 17 of 118
            Pseudo Genes (multiple problems)  :: 11 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043457.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="18WIARLN0025"
                     /isolation_source="Skin/Wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WI"
                     /lat_lon="43.78 N 88.78 W"
                     /collection_date="2018"
                     /collected_by="Wisconsin State Laboratory of Hygiene"
     gene            1..951
                     /gene="repM"
                     /locus_tag="FZ920_RS19665"
                     /old_locus_tag="FZ920_19830"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="FZ920_RS19665"
                     /old_locus_tag="FZ920_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="FZ920_RS19670"
                     /old_locus_tag="FZ920_19835"
     CDS             944..1519
                     /locus_tag="FZ920_RS19670"
                     /old_locus_tag="FZ920_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="FZ920_RS19675"
     CDS             1539..1682
                     /locus_tag="FZ920_RS19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1865..2692)
                     /locus_tag="FZ920_RS19680"
                     /old_locus_tag="FZ920_19840"
     CDS             complement(1865..2692)
                     /locus_tag="FZ920_RS19680"
                     /old_locus_tag="FZ920_19840"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            3001..3288
                     /locus_tag="FZ920_RS19685"
                     /old_locus_tag="FZ920_19845"
     CDS             3001..3288
                     /locus_tag="FZ920_RS19685"
                     /old_locus_tag="FZ920_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3275..3589
                     /locus_tag="FZ920_RS19690"
                     /old_locus_tag="FZ920_19850"
     CDS             3275..3589
                     /locus_tag="FZ920_RS19690"
                     /old_locus_tag="FZ920_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3717..6128
                     /gene="znuD2"
                     /locus_tag="FZ920_RS19695"
                     /old_locus_tag="FZ920_19855"
     CDS             3717..6128
                     /gene="znuD2"
                     /locus_tag="FZ920_RS19695"
                     /old_locus_tag="FZ920_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6433..6894
                     /locus_tag="FZ920_RS19700"
                     /old_locus_tag="FZ920_19860"
     CDS             6433..6894
                     /locus_tag="FZ920_RS19700"
                     /old_locus_tag="FZ920_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7220..7429)
                     /locus_tag="FZ920_RS19705"
                     /old_locus_tag="FZ920_19865"
     CDS             complement(7220..7429)
                     /locus_tag="FZ920_RS19705"
                     /old_locus_tag="FZ920_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(7422..7724)
                     /locus_tag="FZ920_RS19710"
                     /old_locus_tag="FZ920_19870"
     CDS             complement(7422..7724)
                     /locus_tag="FZ920_RS19710"
                     /old_locus_tag="FZ920_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7717..8073)
                     /locus_tag="FZ920_RS19715"
                     /old_locus_tag="FZ920_19875"
     CDS             complement(7717..8073)
                     /locus_tag="FZ920_RS19715"
                     /old_locus_tag="FZ920_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269902.1"
                     /translation="MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKGGKKRFYKEMLDIADQQ
                     YELHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8682..9509
                     /locus_tag="FZ920_RS19720"
                     /old_locus_tag="FZ920_19880"
     CDS             8682..9509
                     /locus_tag="FZ920_RS19720"
                     /old_locus_tag="FZ920_19880"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(9553..9744)
                     /locus_tag="FZ920_RS19725"
                     /old_locus_tag="FZ920_19885"
     CDS             complement(9553..9744)
                     /locus_tag="FZ920_RS19725"
                     /old_locus_tag="FZ920_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     ARG             complement(1869..2692)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8683..9506
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa ggacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa cccgactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga
      661 ttttctttta cctttaaaca gaagaagaag gatagcccat caatagaaag agatccgaac
      721 actttggagc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaacag aaagctacca acagtttgct
      841 gtacgtattg ctgagatgct acaagatccc gctcaattca aagagctata cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggt gtattatgtt aattttagaa
     1861 attattaaat gattccaaga ttttctagcg acttataagt aatttcatta cgaatagaac
     1921 cagacattcc ttctttcatt tctaagttga gcgaaaaggg gatttttttt ccattagctt
     1981 gctccaccca accagtcaac caacctacct gtggagtaac atccattccc catccacttt
     2041 ttgcataaat cttactacca tttacttctt taattagaag cattttttta acttcttctt
     2101 gagtttctaa tttaaaaggt aatcggttat gtgcaaggtc atcggcaaaa ttaacttctt
     2161 gtactggtgt aattttaagg gggccaacta accaaaaatt atcgacctgt gttccaatat
     2221 ttgtatttcc aaaattaacc cgctttactt ctttctgcat tagctctagg ccagtccgtc
     2281 ttgcaagctc ttgatatact ggaactgctg acaatgccat tgcctcacct aaagtcatat
     2341 ctttctccca cataggataa gttctttttt taccatccca tttgaaaatc tcatttgttg
     2401 ttgctttatg attttctagc ccgattaaag catttagcat cttaaatgtt gatgcaggga
     2461 catattcttt atttgctcgt gcaagagcat taccataggt gctaagattt ttaccctctt
     2521 taataataat tacaccctgt gtttgagctt catcaaaata gcttttaata gctttttcat
     2581 gttgctgaga agaaatatga aaattatctt cagatttagt tttaatagat gaacatgcac
     2641 tgagagaaac tagaatagaa atgctgaata taggaagtat aaattttttc attacaaatt
     2701 catgttaggg gaaattttgg ggcttagagc ataatatttt tattaaaaaa gttaaaactc
     2761 ttttaattta acataatggc gattatacga agtacacttg tatattaata taaatatcaa
     2821 ctacttaata aaaatagaaa aatcccaaaa tgcccaaaaa gccgactttg aaacaagtcg
     2881 gcttttttat gagcagtttt gatacttctt gccaataatt ttgactaaaa attacacgaa
     2941 tctggttgag tgatacaatt ttgtgataca ttattgtata tacaaatttg aatgtttttt
     3001 atggaacagt attttgaatg ggatgaggct aagaatcgaa agaatcagaa aaagcatgat
     3061 atctctttcg aaactgcaag ccttgttttt gaagatccat tacggatctc aatccaagac
     3121 agacatacca atggtgaaga acgttggcaa accattggac gagttaaagg cgtactaatg
     3181 ttattggtgg ctcacaccat ttttgatgaa gatgactgtg aaatcatccg aatcattagt
     3241 gcaaggcaag tcactaaagc ggagcgaaac aaatatgagc atggttagat actcacacaa
     3301 agagctgaat gagaaatttg gtgagaagca agatgctgaa attcaacgct tgcttgctaa
     3361 aggcacggtt cctgatgatc agctagatct atcagatatt cctgaaatta cagactggag
     3421 caatgctgta cgccaaaatc aattttaccg cccagtaaag caacaaactt caattcggtt
     3481 agatgctgat gtacttgctt ggttcaaagc acaaggcaaa ggttatcaaa cacgtatgaa
     3541 tgaaatttta cgagatgcta tgctaaaaga attaaaaaat catcaataag aatgggagct
     3601 taggctccca tttttgactt catttttagg ggggattacc attcaaaaat ttaaatagta
     3661 gattttataa atttttaagt gttatattat aatataacat taaatttggt tgttttatgc
     3721 taaataaaag taaactattt ttagctttaa ttactttagg ggcctcgaaa attttattgg
     3781 cagctgaagg gcctgttact accttaaata caatagtatt aactgcacaa tctgatgagt
     3841 tagggtctga gctgttaggc aaaagtctaa atgttagtaa tcagtttatt gatactagta
     3901 aacttaaaca gcgttcgaca actctagggg atgccttggg tacggaatta ggaattcatt
     3961 ctaatcaata tggaggtgga gcatctactc ctattattcg tgggcaggaa ggaaaacgta
     4021 ttaaagtttt acagaataat gctgacgtgt tggatatgtc taatatgtcc ccagatcacg
     4081 ctgtcacagt tgaaccaagt ttagctaagt ctattgaaat tatacgtggt gcaagtaccc
     4141 ttttgtatag ttcaaatagt gcggcagggg tagtcaatgt cattgactat aaaattccaa
     4201 cccaaatgcc tcaagacgga ttagagggta atactacatt acgttttaat actggcagta
     4261 atgagaagtt gaccacagca ggtgtaacag ttggcttaag cccacatgta gctttacggg
     4321 cagaagggtt atatcgtaat gctggtaact ataaaactcc tcattaccag tctagctctt
     4381 ataattcttt agaagatctt gaaaaccaaa acagtattta taaaaattta aaatatttac
     4441 cagaaagctg ggcagagtcg agagtaggta cgcttggatt atcctggata gacgataata
     4501 cttatttagg agtttcttat acacatcgtc atgatgaata tggtttacca gcccatagcc
     4561 atttatatga aggttgtggt gcctctgcaa tcggtatcga ttcacgaatt agtggattaa
     4621 aaaattatct tttatattat cctcaattga tggatgagca agatattaat tacataaatc
     4681 cacgtcctga ttgtcatcag cataatcata ttcatgaaac taatttctct cataatgctc
     4741 cttatattga tttaaatact cgacgttatg atgtgcgtgg agaattcact caaccattta
     4801 ccggtattga caaaattcgt acctcattga gttatataga ttattttcac aatgaactcg
     4861 agggagataa gattactaac ttttttaaaa atacaggaaa agtaggccgc atagagttat
     4921 cacatcagcc attaggggaa ttaaccggta tcttggggct acaatatttg gaacaggata
     4981 atagtgcttt atcaccagtc cattctcaag aaggccatac cacctatcta gataatcagc
     5041 agctattaaa tcgaaatgtc actaaaaact ttagtgtatt tggcctagaa aaatataact
     5101 ggaacgactt tactttcgaa ctaggtgctc gtatagaaaa gcagaaagtt agtatggatt
     5161 atgatataga aaaaattaaa gattcaatga aaccttggcc taataaatat aacagtcctt
     5221 atgtagaaaa aaataataaa atcagggctc aaaatttaaa atctatttta gaagctgttc
     5281 aacctaacaa agaaacagct ttttcttatg caggtacagt ccactggaga tttgctccaa
     5341 attatatctt atcattaaca ggtacacatc aagaaagatt acctaatgca caagaaatgt
     5401 atactcatgg tatgcatctc gctactaatt cttttgaaat aggtaaccgc tttctaagaa
     5461 aagagaaatc caataattta gaaatcagtc tggcctataa ggatgattta cttgattatc
     5521 aaatcagtac ttactattat gattttgata actatatata tttacagaca ctcaatgaag
     5581 ttttaggaac tacaaaagtt agagatcagc atacactacg tattaatcat tatagccagt
     5641 ctgctgctaa tttttatgga ttagagggca acatagggta tcaatttaat tctgtttatc
     5701 acggctctct ttttggagat tatgtgaaag gccgtttaac aaatttacct gatgctgtaa
     5761 ttgcatacga tatctggaat agagaaccga ctttagctcc ccaaaaggat cgttacactc
     5821 ctagattacc tcctgctcgt ttgggtacac gactaaaagc tgattttgat gaatcgctta
     5881 aaggtgaaat agaatattat cgagtcttta aacaagataa tatttctaaa tttgagcagg
     5941 ttacatcagg ttataatatg cttaatatga ctttagcata taaaaataag ctatctcaca
     6001 cagaatatga tttattcttt aaagcgaata atttacttga tcagaaagtc tatgcccatg
     6061 aaacattttt gccatatatt ccccaaattg gaagaaattt tagtttaggc ttaaatctta
     6121 atttctagat aagatactgt aatcttctaa gaaaatttct tagaagattt ttcttataaa
     6181 atgattagat taataattaa ggtaagcgtc cgctgaatcc catacctatt ttttaattcg
     6241 atttaggttt ccagcagtgt ggcagtaact cttcaatctg ggtcacttta tgtgtcggca
     6301 gccttttcag cactgaatag ccaccgattt tgtagacacc ttttagctag ataaaaagca
     6361 atttttttga tctttaaaga atgaggatat aagctgttta aaacttaatt ctaattggtt
     6421 aataggtgaa aaatgaaatt agcagaagct ctcttacttc gaagtgatca acaaaaaaaa
     6481 attatttcac taaaacaacg tatcaatgca aatgtattgg tacaagatgg tgatcaacca
     6541 tctgaagatc caaatgaact actaaaacaa gtattttctt taattcaaga atttcaaaaa
     6601 ttaagttatg caattcatga aactaatgct ttaacaaaat taaatgatgg ccgttctcta
     6661 cttgctttat tgactttgcg tgatgaattt gtagaacagc acaaaaccct aactgcagca
     6721 ataagtaata catctcgtga atctgatcgt tatagtacgc gggaaataaa gtggcataaa
     6781 gttattccag tttcatcatt acaaaaacaa gctgatgata ttagtcttaa attacgtgat
     6841 ttaaacgtgt taattcagtc gactaattgg aaaattgatt tgattgaagc ctaatagtcc
     6901 tttagatata atcaagttga acatactggg cgagtacaag atcccttact tccacaacat
     6961 aggattgtaa ataaagtgta gtggcttgca aagcaagtgg ataagcttaa aattttaagt
     7021 acaaatcaat ccatcacttg gcataactca aatctttgtg caaacaaagt tacacgttac
     7081 ttataactac catgtactga cagtatgttc acctcaatta ctaaaaataa agaaatgaac
     7141 aagcatcagc tcaactacat ttctattttt aattcagttc ggcatttaac ataaaatctc
     7201 ttatgcgaat ctggaaggat taagctattt cgtaatattt tatccaataa gcatcgtcaa
     7261 aaatatcaat gtaatagctt gacctcttac aatttgaaaa atagtcacag cataaatctc
     7321 taataatttc aatttcttct aggctatcgg cagcattgac taaaaatatg aacttgtcca
     7381 agttaacaag taatttagta ctatttttca aaggtttatt attagacatt aaatcctatg
     7441 tgttttcctg ttggaagctc tacatcaata cgaagttttc cgcccattgc ttcaacatac
     7501 tttttcatgg ttgaaatttt aagatcatga ccacgatttt cgattgctga caatgacggc
     7561 tgtttgattc caagggttgc agccagttct ttttgagaaa tctctaactc ttcacgaata
     7621 aggtgaagct gagtttccaa cagtaattca tcagccattt gttgaatacg agcttgactc
     7681 tctggagagc gactagccaa taattcttgt agggtcttag ccattagatt gatctcctaa
     7741 agttgagaga tgaagttcgt attgctgatc agcaatatct aacatctctt tataaaaacg
     7801 ctttttaccg cctttatcac cgatacacag cacaatagct tgtcgtaaag gatcaaatgc
     7861 gaaaaaggct ctcagtggtt tacctcgatg ctgaacacgt agctctttca tattggtaaa
     7921 tttggagtca tagacggtat ctactaaagg acgacctaaa cttggtccct gttgctgtag
     7981 aacgaccaac gctgcgagta ctttctcttg tgtagattca tcttgctgtg caagccattc
     8041 gttaaacaga tccgttgtaa tgactgtcca catattacaa ccataatata gattacagtc
     8101 tatattttat gcttaattga attattattc aaatgacaat aaaaaagccc gacatcctgt
     8161 ctggcttttt cgtttttgca tctcaagtgt aacttctact ttgcttcacg atcctagtgc
     8221 gctcacttat gatagttgtt ttgtgtgctg gagcatcctg ctctctatgt gtccatgata
     8281 gaaaattaag ctgagtctaa ctattggtta ataccctcaa tcggtgtgcg cttaggcgta
     8341 cataattggc gtatatttat cccaagattt atccgcaaat tttgtggatt atttcaaaat
     8401 gctgactgtt gagaaatcaa agaaaactga atttttgaat attttttaat ctattttatt
     8461 ggctaaaagt gacaaagaag cttaaaaaat acccaacttg tttccgagtc ggcattctgg
     8521 tattttttgg cgttatgtag aatacataaa tatttgattt aattaaaata atttaaattg
     8581 atttcgcata acgcccatta tgttaaatta aaagagtttt aactttttta ataaaaatat
     8641 tatgctctaa gccccaaaat ttcccctaac atgaatttgt aatgaaaaaa tttatacttc
     8701 ctatattcag catttctatt ctagtttctc tcagtgcatg ttcatctatt aaaactaaat
     8761 ctgaagataa ttttcatatt tcttctcagc aacatgaaaa agctattaaa agctattttg
     8821 atgaagctca aacacagggt gtaattatta ttaaagaggg taaaaatctt agcacctatg
     8881 gtaatgctct tgcacgagca aataaagaat atgtccctgc atcaacattt aagatgctaa
     8941 atgctttaat cgggctagaa aatcataaag caacaacaaa tgagattttc aaatgggatg
     9001 gtaaaaaaag aacttatcct atgtgggaga aagatatgac tttaggtgag gcaatggcat
     9061 tgtcagcagt tccagtatat caagagcttg caagacggac tggcctagag ctaatgcaga
     9121 aagaagtaaa gcgggttaat tttggaaata caaatattgg aacacaggtc gataattttt
     9181 ggttagttgg cccccttaaa attacaccag tacaagaagt taattttgcc gatgaccttg
     9241 cacataaccg attacctttt aaattagaaa ctcaagaaga agttaaaaaa atgcttctaa
     9301 ttaaagaagt aaatggtagt aagatttatg caaaaagtgg atggggaatg gatgttactc
     9361 cacaggtagg ttggttgact ggttgggtgg agcaagctaa tggaaaaaaa atcccctttt
     9421 cgctcaactt agaaatgaaa gaaggaatgt ctggttctat tcgtaatgaa attacttata
     9481 agtcgctaga aaatcttgga atcatttaat aatttctaaa attaacataa tacaccttat
     9541 gcgaagccca gattaatcct gggctttttt ttcaatttgc ttattagctg taaacattcc
     9601 aggattagtg gttcggatgt tttcctgaca gctactacaa agcattaacc ctgcaaaaag
     9661 ttgtacattt tcctttgatt cacagaatcc acatgattta ggaaaaggaa cgatatttgc
     9721 taagttgact aattcttttg acataatgtt tatcctcaac ttgtacatat taatttcaat
     9781 atttttcagt atgttatacc taatttcacg ttaaactttg tacattcttg tataatccat
     9841 gtgtacaaac tcttttttat aaatttcata tatttaagcc taaaaaacca ttaatttatg
     9901 tacaacacta tgtacatact aaaaactata taaatcaaaa aattagtatt aaaaagatca
     9961 attattttgt acacaattca gaacctataa aaacaaccaa aaaccttatg aaataaagct
    10021 ttgaaggtca tcatctttag atgcgtggtg tgtgacaaaa aaatcccggc atgtgccgga
    10081 tcttggattg gaaaattggc taaagtgacg tagggccggt gcttggtttt gcatggaaaa
    10141 aagtatttat ttccttgttt agaaaaacgt aaaaaatcaa ttttttgtcc attcatccag
    10201 gttaaaaatt tcaacccaaa actttaatta tgaaaagctt catagaacgc gttcaaatgc
    10261 gatttaagag cctttaccta gaaaacatag atctcatagt gaaaaacaca aaacagctca
    10321 aaaaacgcaa atgagagcga aataaagagg tattttgact ttagatagat gcatagagcg
    10381 agtgtctacg agcgaactat caaaatttgc gcctagaccc tctgaaaaac atttttttgt
    10441 cctctttagc ctaagaaagc gtaagtttcg tgcagaaatt tgctcctgga tcgagcgtag
    10501 cgagtaaaaa agttttatga gcgaagcgaa ttccaagttt cttttgcttt tttttaaagt
    10561 cacgccagta ttaaccaaaa aattgcccca acgaactgag cgaaagcgaa gttcaataga
    10621 gtttgagcga agcgaaaacc aagggcaatt tttcattccc tgggctttta attattttaa
    10681 agtttttaaa tacttttaaa tggcttgaaa gccttatgaa taaagggttt tagctcttat
    10741 atgtccacgt ttaccttgca atatgtccac gtttaccttg caatatgtcc acgtttacct
    10801 tgcaatatgt ccacgtttac cttgcattag tacacaaata atattaacgt gtacttatac
    10861 acaataaaaa atagtggct
//
