LOCUS       NZ_CP043412             1934 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain NMBU-W13E19 plasmid pNMBU-W13E19_06,
            complete sequence.
ACCESSION   NZ_CP043412
VERSION     NZ_CP043412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12640810
            Assembly: GCF_013282335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:47:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,247
            CDSs (total)                      :: 5,126
            Genes (coding)                    :: 4,919
            CDSs (with protein)               :: 4,919
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 56 of 207
            Pseudo Genes (incomplete)         :: 158 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            Pseudo Genes (short protein)      :: 4 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043412.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W13E19"
                     /serotype="O25:H18"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pNMBU-W13E19_06"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Oda Ytreoy"
                     /genotype="ST69"
     gene            complement(419..1405)
                     /locus_tag="FZN32_RS25935"
                     /old_locus_tag="FZN32_26680"
     CDS             complement(419..1405)
                     /locus_tag="FZN32_RS25935"
                     /old_locus_tag="FZN32_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(1392..1694)
                     /locus_tag="FZN32_RS25940"
                     /old_locus_tag="FZN32_26685"
     CDS             complement(1392..1694)
                     /locus_tag="FZN32_RS25940"
                     /old_locus_tag="FZN32_26685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          502..693
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
ORIGIN
        1 ggcctcactt cgtgaatcca accacctcag ccaatttttc aatcagcaat gtcgtcaatc
       61 actaaatcgg aacccactgg caagccagcg ggaccccacg atttagagtt tgacgatttc
      121 ggaaacggcg agcgctggca gattttaatc tgactgcgtg aggtgttggc agggtgtcgg
      181 ggcgcagccc tgacgaatcg tttttggcac cgacatcgtg atggcggtgt accaaaaaca
      241 catcttgtcc caactgagtt cggaaaccga actcagtaat tgagtgacgt cactttgcgg
      301 cgttagccct atcctgcatc ggagtagaac caataggaca agatgtgttt ttggagtacc
      361 gccgacaagc ggcgccctct caaaaacgtc gtggtcaggg ctgcgccccg acaccctgtc
      421 agtctgattc agctttatct tttgacggtg agcgacggta ctttttggat tcggttttcc
      481 actcaaaagc aatgcgtgaa ccatcatcat cgccttcggg aatatcatcc ccgattacca
      541 tatcggcatc agtttcctga tcgagtttca gtatgtcttt gagtgcgcca ccggtagcca
      601 caaatcggcg cttatgtgtc tgcttagtca attcgaggaa ccattcagga tcagcaacca
      661 tgtcagccgg cttcgtggaa tatttcaggg tttcagccac tgcgccccgt acaagttccg
      721 cagttgcaga agccaatgat tctccgtcct taggcttgcg gggttttact gctcgaacat
      781 caacattcgg atcgtaattc acacgcaaac actctcgcca aagctctacc cagcggtcat
      841 gtttaacgta atctcgcgtg aacattgagg gaggaaccat catcagagtg tgaaagtgcg
      901 ggtgcgcact accgtcactt ccacgtgtaa cttccgtggt gcggatccag ccctgaacag
      961 gtcgaaactc tttgcgatcc ttaagtcgct ggaaggccat gttcattcgg ttaagtgtct
     1021 cacccaattc cccgatcgcg cagtttcgca ctgtgagggt caggaacatc cagcgtgcat
     1081 ctggataatc cgcaacaata cgcggcaggg actgataaaa acgggcttgc cacatcaggg
     1141 atcgcctcca ctggcagaca ggacagtgcc ggacacggca gaaatgcgcc tcacgtagcc
     1201 taaggcgagt ttctccagtt tctttgaggg tagaccaacc aaacctcagt aaaccgccac
     1261 aggaagccat acgcgccccg tagcgctcat attcggctgc gagaaggtaa atcccaccta
     1321 cgtcgtcaga aacgcttttg tgagcgtccc agggcgcgtc tgagggggag taatccgtga
     1381 ggttctggag tttagggttt tgcatgcaca ccgtcccatg ctgggcgatg gctcatttga
     1441 cgtgttatca ttgccgtatc acctgttggt tgtcgcttcg aatcgaattt ctgcaggtgg
     1501 tcacaccccc gaacgatggc cgtcgttgcg ggggtcgctt ttaaggcgac cactcccaac
     1561 agaatacttc caaaaccttc tacaacgcaa ataagccccg aagccttggt acgcaaggct
     1621 tgatgccatt ttttatcgcc agagttatcc cttaagtata aatacaagga tcgcgcgcgg
     1681 atcgaaccag ccattcaacg cccacccgcc accaaaaact gccacaacgc caggagcgaa
     1741 acagcaacgc tcaacgcact atcaacatga ttaagaacct caaaaaacat aacaacctca
     1801 ctcgaatgga attgggtaaa tctatttagt ctgtgatttg cgaacccata ctttttcgca
     1861 tcacgacaga accaactcca ttcgttctgg ttgtatccgg taccagacaa ttaaaaaccg
     1921 tcttccaggt ggtt
//
