LOCUS       NZ_CP043411             2101 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain NMBU-W13E19 plasmid pNMBU-W13E19_05,
            complete sequence.
ACCESSION   NZ_CP043411
VERSION     NZ_CP043411.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12640810
            Assembly: GCF_013282335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:47:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,247
            CDSs (total)                      :: 5,126
            Genes (coding)                    :: 4,919
            CDSs (with protein)               :: 4,919
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 56 of 207
            Pseudo Genes (incomplete)         :: 158 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            Pseudo Genes (short protein)      :: 4 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043411.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W13E19"
                     /serotype="O25:H18"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pNMBU-W13E19_05"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Oda Ytreoy"
                     /genotype="ST69"
     gene            196..1161
                     /locus_tag="FZN32_RS25925"
                     /old_locus_tag="FZN32_26665"
     CDS             196..1161
                     /locus_tag="FZN32_RS25925"
                     /old_locus_tag="FZN32_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1165..1431
                     /locus_tag="FZN32_RS25930"
                     /old_locus_tag="FZN32_26670"
     CDS             1165..1431
                     /locus_tag="FZN32_RS25930"
                     /old_locus_tag="FZN32_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          590..821
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ttttgacagg tcaaagaaaa tggagcagaa ttgaggcgtt tttaatcggc gttggggagt
       61 gcgtcaacac tccccaacat ttcgaatgtg tcacctcagc ggcaaactct ggtgacatgt
      121 actggctcgc aatgcacagg tacgtgatga atataccaca tcaaatcaca gcctgcccag
      181 atcggagcag gcttaatgtc agaagataaa ttcctttcgg actacagccc ccgtgatgca
      241 gtttgggata cccagcgcac gcttaccgat tctgtcgggg gtatctacca gactgctgct
      301 gaattcgagc gctatgcact ccgtatggcc tcctgtagcg gtttgttacg ttttggttgg
      361 tctaccatca tggaaaccgg agaaacgcgc ctacggcttc gtagtgcgca attttgccgt
      421 gtccgtcatt gccctgtctg ccagtggaga agaaccctca tgtggcaagc ccgtttttat
      481 caggctctac cgaaaatcgt tgtggattac ccgtcttccc gatggttgtt tctgacgtta
      541 actgtcagga actgcgagat aggtgaactt ggaacagtcc ttacagcaat gaatgcggcg
      601 tttaagcgaa tggaaaagcg aaaggagcta tcacctgttc aggggtggat cagggctacg
      661 gaggtgacgc gaggtaagga tggcagcgca catccgcatt ttcactgtct gctgatggtg
      721 caaccttctt ggtttaaagg gaagaactac gttaagcacg aacgttgggt agaactctgg
      781 cgcgattgct tgcgggtgaa ctatgagccg aatatcgata ttcgggcagt aaaaactaag
      841 acaggtgagg ttgtggccaa cgttgccgag caactgcaaa gcgcggttgc tgaaacgctg
      901 aaatactccg ttaaaccgga agatatggca aacgatcctg agtggtttct tgagctgacg
      961 cggcagcttc acaagcgccg ttttatctcg accggtgggg cgctaaaaaa cgtcctccag
     1021 ttggatcgag aaaccaatga ggatcttgtc attgccgacg atgtagggga tggcactgat
     1081 gacgggaagc ggacggcgtt tgtctgggat tcaggtaaac ggcgttacaa acgcgcccct
     1141 gagaaggata aatcggatta acgtatgaat attaatattg aatacctgaa tggaaataag
     1201 actattggtt tatttttttt aagaagtgaa gcggtgattc ctgacaggtt taaaaacctt
     1261 attttgctta ttgatggatt aagttttggc acatttggtt ttcatccgca cgaaggtttt
     1321 gaggatgaat taattttata tattcagaaa acaaacgaga gggtaaaaac tctttttgtg
     1381 aaaatagatt taaacggtat caagtttgga tttttaagaa cgcattctta gttctggaaa
     1441 agagccagcg gcaggctgag gtgataggta cgagattgca tgcaatctct agtgctctgt
     1501 ctatcctgca ttatcctcag cattatcctc agcattatcc tcagccttgc caactcgaca
     1561 ccaatgcagg atagacaatc cgatgtcaaa tgttaacact ctgcgagtgg tacattttcc
     1621 ccggattatc gtcctgagcc tgccgctggc tctctttcta ccgcctcgct ttgctcgttg
     1681 ctcaacgcct cacagacacg gattaaaatc cgcatccgtt caccgttttt taaagtccgt
     1741 taaaagcatg atgccatctc cgagagttaa tctcgtcaaa tgctaaatcg tgggggtccc
     1801 ctttggggtt ccgatttagt gattgacgac accaccgatt aaaaaactta tgcggggtgg
     1861 atggtttcac gaagtgaggc catccacctg taagacaggg ttttgttttt attccctgtt
     1921 ttggtgatcg ggtgtgtgga aaaggttggg gtaagccgtt cgggggtgct tgttttgggg
     1981 ggttaaaatt gtggttattt tttgcgcaat tctcgcgcgt gatccttgta tttatactta
     2041 agggataaat ggcggatatg aaatagtggt ttagcccagt aatgacgagg ctttgagtgg
     2101 g
//
